P38770 (BRL1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Nucleus export protein BRL1

Alternative name(s):
BRR6-like protein 1

Gene names

Name:	BRL1
Ordered Locus Names:	YHR036W

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	471 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Involved in mRNA and protein export from nucleus. Ref.4
Subunit structure	Interacts with BRR6. Ref.4
Subcellular location	Nucleus membrane; Multi-pass membrane protein Ref.4.
Miscellaneous	Present with 1280 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the BRL1/BRR6 family.

Ontologies

Keywords
Biological process	Protein transport Transport mRNA transport
Cellular component	Membrane Nucleus
Domain	Transmembrane Transmembrane helix
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	mRNA export from nucleus Inferred from mutant phenotype Ref.4. Source: SGD nuclear envelope organization Inferred from mutant phenotype Ref.4. Source: SGD protein export from nucleus Inferred from mutant phenotype Ref.4. Source: SGD
Cellular_component	endoplasmic reticulum Inferred from direct assay Ref.4. Source: SGD integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW nuclear envelope Inferred from direct assay Ref.4. Source: SGD nuclear membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 471	471	Nucleus export protein BRL1		PRO_0000202892
Regions
	Topological domain	1 – 299	299	Cytoplasmic Potential
	Transmembrane	300 – 320	21	Helical; Potential
	Topological domain	321 – 407	87	Perinuclear space Potential
	Transmembrane	408 – 428	21	Helical; Potential
	Topological domain	429 – 471	43	Cytoplasmic Potential

Sequences

	Sequence		Length	Mass (Da)
	P38770 [UniParc]. Last modified February 1, 1995. Version 1. Checksum: 732209EC74DE3463	FASTA	471	53,385
	10 20 30 40 50 60 MESFENLSIR DSFTSGMEHV DEELGGLSDL SISKQGPTLS PQLINRFMPH FPSSPSPLRN 70 80 90 100 110 120 TLDFSAAKAD EEEDDRMEID EVDDTSFEEE YNNEPIETHT EATENAVVEE IEATPEERQK 130 140 150 160 170 180 QEKNESQDQS VEEVENIVSP HRSTVIKALL SPTDLGVAAA TKVEGVVPLP PSANQDDNES 190 200 210 220 230 240 SNNNAEGEDI IRNEEVEDEI KSSLGNHKSS QYANAFDSEI IKRELRSRSK YQPIQVSFNT 250 260 270 280 290 300 HNYFYSDKDG IKTYSLTKPN HNKIDEFYDQ NEAFKLPKPW SPNSHPASRA SYALMSYLQL 310 320 330 340 350 360 FLNAITTVVI FSFILSFIIA LQKDLKSTWE QRKHELQYES RICQEQYLTN RCNQTPGLPA 370 380 390 400 410 420 LGEQCAIWKQ CMDRNNDIFF RARSTLSAKL FGDIINSFID PLNWKTLFVI FCGVITWCFS 430 440 450 460 470 SNFLLGFVRA KSYYGNGIKT YPLPSSPKSP TSEETHSSMT ASGEDSHLLK Q

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete nucleotide sequence of Saccharomyces cerevisiae chromosome VIII." Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Dover J., Du Z., Favello A., Fulton L., Gattung S., Geisel C., Kirsten J., Kucaba T., Hillier L.W., Jier M., Johnston L., Langston Y., Latreille P. , Louis E.J., Macri C., Mardis E., Menezes S., Mouser L., Nhan M., Rifkin L., Riles L., St Peter H., Trevaskis E., Vaughan K., Vignati D., Wilcox L., Wohldman P., Waterston R., Wilson R., Vaudin M. Science 265:2077-2082(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[3]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[4]	"Brl1p - a novel nuclear envelope protein required for nuclear transport." Saitoh Y.-H., Ogawa K., Nishimoto T. Traffic 6:502-517(2005) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH BRR6.
[5]	"A global topology map of the Saccharomyces cerevisiae membrane proteome." Kim H., Melen K., Oesterberg M., von Heijne G. Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed] [Europe PMC] [Abstract] Cited for: TOPOLOGY [LARGE SCALE ANALYSIS]. Strain: ATCC 208353 / W303-1A.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U00062 Genomic DNA. Translation: AAB68908.1. BK006934 Genomic DNA. Translation: DAA06727.1.
PIR	S46739.
RefSeq	NP_011901.1. NM_001179166.1.
3D structure databases
ProteinModelPortal	P38770.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-5580N.
IntAct	P38770. 12 interactions.
MINT	MINT-526264.
STRING	4932.YHR036W.
Proteomic databases
PaxDb	P38770.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YHR036W; YHR036W; YHR036W.
GeneID	856431.
KEGG	sce:YHR036W.
Organism-specific databases
CYGD	YHR036w.
SGD	S000001078. BRL1.
Phylogenomic databases
eggNOG	COG5085.
GeneTree	ENSGT00530000066593.
HOGENOM	HOG000095263.
OMA	IEPLGWK.
OrthoDB	EOG4WM845.
Gene expression databases
Genevestigator	P38770.
GermOnline	YHR036W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR018767. Brl1/Brr6_dom. [Graphical view]
Pfam	PF10104. Brr6_like_C_C. 1 hit. [Graphical view]
SMART	SM01042. Brr6_like_C_C. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	982021.

Entry information

Entry name

BRL1_YEAST

Accession

Primary (citable) accession number: P38770
Secondary accession number(s): D3DKY3

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	February 1, 1995
Last modified:	April 3, 2013
This is version 83 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program