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Protein

ATP-dependent DNA helicase RRM3

Gene

RRM3

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

5' to 3' DNA replicative helicase recruited to paused replisomes to promote fork progression throughout nonhistone protein-DNA complexes, naturally occurring impediments that are encountered in each S phase where replication forks pauses. Needed for normal fork progression through over 1000 discrete sites scattered throughout the genome, like rDNA, tRNA genes, centromeres, active replication origins, or transcriptional silencers. Required for timely replication of the telomere and subtelomeric DNA and for wild-type levels of telomeric silencing. Involved in regulation of Ty1 transposition and protects the genome from instability at nascent sites of retrotransposition. Involved in DNA repair during stalled replication fork, regulation of fragile sites expression and essential for genome stability. Plays also a role in mtDNA replication. Has G-quadruplex (G4) unwinding activity and can suppress G4-induced genome instability when PIF1 levels are low.24 Publications

Miscellaneous

Present with 656 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

ATP + H2O = ADP + phosphate.UniRule annotation

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi254 – 261ATPUniRule annotation8
DNA bindingi682 – 701UniRule annotationAdd BLAST20

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • ATP-dependent 5'-3' DNA helicase activity Source: GO_Central
  • ATP-dependent DNA helicase activity Source: SGD
  • DNA binding Source: UniProtKB-KW
  • DNA helicase activity Source: SGD
  • telomerase inhibitor activity Source: GO_Central

GO - Biological processi

  • DNA repair Source: UniProtKB-KW
  • DNA replication Source: SGD
  • G-quadruplex DNA unwinding Source: SGD
  • mitochondrial genome maintenance Source: SGD
  • negative regulation of telomerase activity Source: GO_Central
  • negative regulation of telomere maintenance via telomerase Source: GO_Central
  • replication fork progression beyond termination site Source: SGD
  • replication fork reversal Source: SGD
  • telomere maintenance Source: InterPro

Keywordsi

Molecular functionDNA-binding, Helicase, Hydrolase
Biological processDNA damage, DNA repair
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-31091-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent DNA helicase RRM3UniRule annotation (EC:3.6.4.12UniRule annotation)
Alternative name(s):
Regulation of Ty1 transposition protein 104
rDNA recombination mutation protein 3UniRule annotation
Gene namesi
Name:RRM3UniRule annotation
Synonyms:RTT104
Ordered Locus Names:YHR031C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VIII

Organism-specific databases

EuPathDBiFungiDB:YHR031C.
SGDiS000001073. RRM3.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Chromosome, Nucleus, Telomere

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi41F → A or D: Reduces the interaction with POL30; when associated with A-42 or D-42. 1 Publication1
Mutagenesisi42F → A or D: Reduces the interaction with POL30; when associated with A-42 or D-42. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001019861 – 723ATP-dependent DNA helicase RRM3Add BLAST723

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei64PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP38766.
PRIDEiP38766.

PTM databases

iPTMnetiP38766.

Interactioni

Subunit structurei

Interacts with DEF1 and POL30.2 Publications

Protein-protein interaction databases

BioGridi36462. 295 interactors.
DIPiDIP-2858N.
ELMiP38766.
IntActiP38766. 5 interactors.
MINTiMINT-1556551.
STRINGi4932.YHR031C.

Structurei

3D structure databases

ProteinModelPortaliP38766.
SMRiP38766.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domaini

The N-terminal part (residues 1 to 249) is essential for function and confers locus specificity.1 Publication

Sequence similaritiesi

Belongs to the helicase family.Curated

Phylogenomic databases

GeneTreeiENSGT00530000063561.
HOGENOMiHOG000132960.
InParanoidiP38766.
KOiK15255.
OMAiNETRIGC.
OrthoDBiEOG092C600K.

Family and domain databases

HAMAPiMF_03176. PIF1. 1 hit.
MF_03177. RRM3. 1 hit.
InterProiView protein in InterPro
IPR003593. AAA+_ATPase.
IPR010285. DNA_helicase_pif1-like.
IPR027417. P-loop_NTPase.
IPR028880. Rrm3.
PfamiView protein in Pfam
PF05970. PIF1. 1 hit.
SMARTiView protein in SMART
SM00382. AAA. 1 hit.
SUPFAMiSSF52540. SSF52540. 3 hits.

Sequencei

Sequence statusi: Complete.

P38766-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFRSHASGNK KQWSKRSSNG STPAASASGS HAYRQQTLSS FFMGCGKKSA
60 70 80 90 100
AASKNSTTII DLESGDEGNR NITAPPRPRL IRNNSSSLFS QSQGSFGDDD
110 120 130 140 150
PDAEFKKLVD VPRLNSYKKS SRSLSMTSSL HKTASASTTQ KTYHFDEDET
160 170 180 190 200
LREVTSVKSN SRQLSFTSTI NIEDSSMKLS TDSERPAKRS KPSMEFQGLK
210 220 230 240 250
LTVPKKIKPL LRKTVSNMDS MNHRSASSPV VLTMEQERVV NLIVKKRTNV
260 270 280 290 300
FYTGSAGTGK SVILQTIIRQ LSSLYGKESI AITASTGLAA VTIGGSTLHK
310 320 330 340 350
WSGIGIGNKT IDQLVKKIQS QKDLLAAWRY TKVLIIDEIS MVDGNLLDKL
360 370 380 390 400
EQIARRIRKN DDPFGGIQLV LTGDFFQLPP VAKKDEHNVV KFCFESEMWK
410 420 430 440 450
RCIQKTILLT KVFRQQDNKL IDILNAIRYG ELTVDIAKTI RNLNRDIDYA
460 470 480 490 500
DGIAPTELYA TRREVELSNV KKLQSLPGDL YEFKAVDNAP ERYQAILDSS
510 520 530 540 550
LMVEKVVALK EDAQVMMLKN KPDVELVNGS LGKVLFFVTE SLVVKMKEIY
560 570 580 590 600
KIVDDEVVMD MRLVSRVIGN PLLKESKEFR QDLNARPLAR LERLKILINY
610 620 630 640 650
AVKISPHKEK FPYVRWTVGK NKYIHELMVP ERFPIDIPRE NVGLERTQIP
660 670 680 690 700
LMLCWALSIH KAQGQTIQRL KVDLRRIFEA GQVYVALSRA VTMDTLQVLN
710 720
FDPGKIRTNE RVKDFYKRLE TLK
Length:723
Mass (Da):81,581
Last modified:February 1, 1995 - v1
Checksum:i8E0010ABB870CAB6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00062 Genomic DNA. Translation: AAB68913.1.
BK006934 Genomic DNA. Translation: DAA06722.1.
PIRiS46744.
RefSeqiNP_011896.1. NM_001179161.1.

Genome annotation databases

EnsemblFungiiYHR031C; YHR031C; YHR031C.
GeneIDi856426.
KEGGisce:YHR031C.

Similar proteinsi

Entry informationi

Entry nameiRRM3_YEAST
AccessioniPrimary (citable) accession number: P38766
Secondary accession number(s): D3DKX8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: October 25, 2017
This is version 147 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome VIII
    Yeast (Saccharomyces cerevisiae) chromosome VIII: entries and gene names