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Protein

26S proteasome regulatory subunit RPN1

Gene

RPN1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Acts as a regulatory subunit of the 26S proteasome which is involved in the ATP-dependent degradation of ubiquitinated proteins.1 Publication

Miscellaneous

Present with 306000 molecules/cell in log phase SD medium.1 Publication

GO - Molecular functioni

  • enzyme regulator activity Source: InterPro
  • protein binding, bridging Source: SGD

GO - Biological processi

Enzyme and pathway databases

BioCyciYEAST:G3O-31087-MONOMER.
ReactomeiR-SCE-1236978. Cross-presentation of soluble exogenous antigens (endosomes).
R-SCE-174113. SCF-beta-TrCP mediated degradation of Emi1.
R-SCE-349425. Autodegradation of the E3 ubiquitin ligase COP1.
R-SCE-382556. ABC-family proteins mediated transport.
R-SCE-450408. AUF1 (hnRNP D0) binds and destabilizes mRNA.
R-SCE-5668541. TNFR2 non-canonical NF-kB pathway.
R-SCE-5687128. MAPK6/MAPK4 signaling.
R-SCE-5689880. Ub-specific processing proteases.
R-SCE-6798695. Neutrophil degranulation.
R-SCE-68949. Orc1 removal from chromatin.
R-SCE-69017. CDK-mediated phosphorylation and removal of Cdc6.
R-SCE-69229. Ubiquitin-dependent degradation of Cyclin D1.
R-SCE-69601. Ubiquitin Mediated Degradation of Phosphorylated Cdc25A.
R-SCE-8854050. FBXL7 down-regulates AURKA during mitotic entry and in early mitosis.
R-SCE-983168. Antigen processing: Ubiquitination & Proteasome degradation.

Names & Taxonomyi

Protein namesi
Recommended name:
26S proteasome regulatory subunit RPN1
Alternative name(s):
HMG-CoA reductase degradation protein 2
Proteasome non-ATPase subunit 1
Gene namesi
Name:RPN1
Synonyms:HRD2, NAS1, RPD1
Ordered Locus Names:YHR027C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VIII

Organism-specific databases

EuPathDBiFungiDB:YHR027C.
SGDiS000001069. RPN1.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Proteasome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00001738152 – 99326S proteasome regulatory subunit RPN1Add BLAST992

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei16PhosphoserineCombined sources1
Modified residuei19PhosphoserineCombined sources1
Modified residuei24PhosphothreonineCombined sources1
Modified residuei178PhosphoserineCombined sources1
Modified residuei187PhosphoserineCombined sources1
Modified residuei695PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP38764.
PRIDEiP38764.

PTM databases

iPTMnetiP38764.

Interactioni

Binary interactionsi

Show more details

GO - Molecular functioni

  • protein binding, bridging Source: SGD

Protein-protein interaction databases

BioGridi36458. 666 interactors.
DIPiDIP-2876N.
IntActiP38764. 130 interactors.
MINTiMINT-707867.
STRINGi4932.YHR027C.

Structurei

Secondary structure

1993
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi485 – 496Combined sources12
Helixi497 – 500Combined sources4
Helixi503 – 513Combined sources11
Beta strandi516 – 518Combined sources3
Helixi520 – 534Combined sources15
Helixi540 – 552Combined sources13
Helixi555 – 558Combined sources4
Helixi562 – 572Combined sources11
Turni573 – 576Combined sources4
Helixi579 – 590Combined sources12
Turni594 – 596Combined sources3
Helixi597 – 605Combined sources9
Turni608 – 610Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2N3TNMR-A482-612[»]
2N3UNMR-A482-612[»]
2N3VNMR-A482-612[»]
2N3WNMR-A482-612[»]
2NBWNMR-A482-612[»]
3JCOelectron microscopy4.80Z1-993[»]
3JCPelectron microscopy4.60Z1-993[»]
4CR2electron microscopy7.70Z1-993[»]
4CR3electron microscopy9.30Z1-993[»]
4CR4electron microscopy8.80Z1-993[»]
5A5Belectron microscopy9.50Z1-993[»]
5MPBelectron microscopy7.80Z1-993[»]
5MPCelectron microscopy7.70Z1-993[»]
5MPDelectron microscopy4.10Z1-993[»]
5MPEelectron microscopy4.50Z1-993[»]
5WVIelectron microscopy6.30Z1-993[»]
5WVKelectron microscopy4.20Z1-993[»]
ProteinModelPortaliP38764.
SMRiP38764.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati417 – 449PC 1Add BLAST33
Repeati450 – 487PC 2Add BLAST38
Repeati488 – 522PC 3Add BLAST35
Repeati523 – 563PC 4Add BLAST41
Repeati566 – 598PC 5Add BLAST33
Repeati778 – 809PC 6Add BLAST32
Repeati810 – 844PC 7Add BLAST35

Sequence similaritiesi

Belongs to the proteasome subunit S2 family.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

GeneTreeiENSGT00550000074983.
HOGENOMiHOG000176022.
InParanoidiP38764.
KOiK03028.
OMAiMVDICAY.
OrthoDBiEOG092C0RGR.

Family and domain databases

Gene3Di1.25.10.10. 1 hit.
InterProiView protein in InterPro
IPR016643. 26S_Psome_Rpn1.
IPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR002015. Proteasome/cyclosome_rpt.
PANTHERiPTHR10943:SF1. PTHR10943:SF1. 1 hit.
PfamiView protein in Pfam
PF01851. PC_rep. 2 hits.
PIRSFiPIRSF015965. 26S_Psome_Rpn1. 1 hit.
SUPFAMiSSF48371. SSF48371. 3 hits.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P38764-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVDESDKKQQ TIDEQSQISP EKQTPNKKDK KKEEEEQLSE EDAKLKTDLE
60 70 80 90 100
LLVERLKEDD SSLYEASLNA LKESIKNSTS SMTAVPKPLK FLRPTYPDLC
110 120 130 140 150
SIYDKWTDPN LKSSLADVLS ILAMTYSENG KHDSLRYRLL SDVSDFEGWG
160 170 180 190 200
HEYIRHLALE IGEVYNDQVE KDAEDETSSD GSKSDGSAAT SGFEFSKEDT
210 220 230 240 250
LRLCLDIVPY FLKHNGEEDA VDLLLEIESI DKLPQFVDEN TFQRVCQYMV
260 270 280 290 300
ACVPLLPPPE DVAFLKTAYS IYLSQNELTD AIALAVRLGE EDMIRSVFDA
310 320 330 340 350
TSDPVMHKQL AYILAAQKTS FEYEGVQDII GNGKLSEHFL YLAKELNLTG
360 370 380 390 400
PKVPEDIYKS HLDNSKSVFS SAGLDSAQQN LASSFVNGFL NLGYCNDKLI
410 420 430 440 450
VDNDNWVYKT KGDGMTSAVA SIGSIYQWNL DGLQQLDKYL YVDEPEVKAG
460 470 480 490 500
ALLGIGISAS GVHDGEVEPA LLLLQDYVTN PDTKISSAAI LGLGIAFAGS
510 520 530 540 550
KNDEVLGLLL PIAASTDLPI ETAAMASLAL AHVFVGTCNG DITTSIMDNF
560 570 580 590 600
LERTAIELKT DWVRFLALAL GILYMGQGEQ VDDVLETISA IEHPMTSAIE
610 620 630 640 650
VLVGSCAYTG TGDVLLIQDL LHRLTPKNVK GEEDADEEET AEGQTNSISD
660 670 680 690 700
FLGEQVNEPT KNEEAEIEVD EMEVDAEGEE VEVKAEITEK KNGESLEGEE
710 720 730 740 750
IKSEEKKGKS SDKDATTDGK NDDEEEEKEA GIVDELAYAV LGIALIALGE
760 770 780 790 800
DIGKEMSLRH FGHLMHYGNE HIRRMVPLAM GIVSVSDPQM KVFDTLTRFS
810 820 830 840 850
HDADLEVSMN SIFAMGLCGA GTNNARLAQL LRQLASYYSR EQDALFITRL
860 870 880 890 900
AQGLLHLGKG TMTMDVFNDA HVLNKVTLAS ILTTAVGLVS PSFMLKHHQL
910 920 930 940 950
FYMLNAGIRP KFILALNDEG EPIKVNVRVG QAVETVGQAG RPKKITGWIT
960 970 980 990
QSTPVLLNHG ERAELETDEY ISYTSHIEGV VILKKNPDYR EEE
Length:993
Mass (Da):109,492
Last modified:January 23, 2007 - v3
Checksum:iB77954F4A998BA7C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U10399 Genomic DNA. Translation: AAB68878.1.
BK006934 Genomic DNA. Translation: DAA06718.1.
PIRiS46779.
RefSeqiNP_011892.1. NM_001179157.1.

Genome annotation databases

EnsemblFungiiYHR027C; YHR027C; YHR027C.
GeneIDi856422.
KEGGisce:YHR027C.

Similar proteinsi

Entry informationi

Entry nameiRPN1_YEAST
AccessioniPrimary (citable) accession number: P38764
Secondary accession number(s): D3DKX4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: January 23, 2007
Last modified: November 22, 2017
This is version 163 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome VIII
    Yeast (Saccharomyces cerevisiae) chromosome VIII: entries and gene names