P38759 (VPS29_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Vacuolar protein sorting-associated protein 29

Alternative name(s):
Carboxypeptidase Y-deficient protein 11
Vesicle protein sorting 29

Gene names

Name:	VPS29
Synonyms:	PEP11, VTP6
Ordered Locus Names:	YHR012W

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	282 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Plays a role in vesicular protein sorting. Required for the endosome-to-Golgi retrieval of the vacuolar protein sorting receptor VPS10. Component of the membrane-associated retromer complex which is essential in endosome-to-Golgi retrograde transport. The VPS29-VPS26-VPS35 subcomplex may be involved in cargo selection.
Subunit structure	Component of the retromer complex which consists of VPS29, VPS26, VPS35, VPS5 and VPS17. Component of a retromer subcomplex consisting of VPS29, VPS26 and VPS35.
Miscellaneous	Present with 259 molecules/cell in log phase SD medium. Ref.5
Sequence similarities	Belongs to the VPS29 family.

Ontologies

Keywords
Biological process	Protein transport Transport
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	protein transport Inferred from mutant phenotype Ref.4. Source: GOC retrograde transport, endosome to Golgi Inferred from physical interaction Ref.4. Source: SGD
Cellular component	endosome Inferred from physical interaction Ref.4. Source: SGD retromer complex, inner shell Inferred from physical interaction Ref.4. Source: SGD
Molecular function	hydrolase activity, acting on ester bonds Inferred from electronic annotation. Source: InterPro protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
VPS35	P34110	3	EBI-13092,EBI-20415

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 282	282	Vacuolar protein sorting-associated protein 29		PRO_0000058305
Amino acid modifications
	Modified residue	207	1	Phosphothreonine Ref.6

Sequences

	Sequence		Length	Mass (Da)
	P38759 [UniParc]. Last modified February 1, 1995. Version 1. Checksum: D190DBA2377E0653	FASTA	282	31,013
	10 20 30 40 50 60 MLLLALSDAH IPDRATDLPV KFKKLLSVPD KISQVALLGN STKSYDFLKF VNQISNNITI 70 80 90 100 110 120 VRGEFDNGHL PSTKKDKASD NSRPMEEIPM NSIIRQGALK IGCCSGYTVV PKNDPLSLLA 130 140 150 160 170 180 LARQLDVDIL LWGGTHNVEA YTLEGKFFVN PGSCTGAFNT DWPIVFDVED SDEAVTSEVD 190 200 210 220 230 240 KPTKENQSED DDAKGGSTGK EQPGSYTPKE GTAGERENEN ESNVKPENQF KEDEVDMSDS 250 260 270 280 DINGSNSPSF CLLDIQGNTC TLYIYLYVNG EVKVDKVVYE KE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete nucleotide sequence of Saccharomyces cerevisiae chromosome VIII." Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Dover J., Du Z., Favello A., Fulton L., Gattung S., Geisel C., Kirsten J., Kucaba T., Hillier L.W., Jier M., Johnston L., Langston Y., Latreille P. , Louis E.J., Macri C., Mardis E., Menezes S., Mouser L., Nhan M., Rifkin L., Riles L., St Peter H., Trevaskis E., Vaughan K., Vignati D., Wilcox L., Wohldman P., Waterston R., Wilson R., Vaudin M. Science 265:2077-2082(1994) [PubMed: 8091229] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[3]	"Analysis of the PEP11 locus in Saccharomyces cerevisiae." Friedrichsen D.M., Brands A., Hiller M.A., Bachhawat A., Jones E.W. Unpublished observations (JAN-1995) Cited for: IDENTIFICATION.
[4]	"A membrane coat complex essential for endosome-to-Golgi retrograde transport in yeast." Seaman M.N., McCaffery J.M., Emr S.D. J. Cell Biol. 142:665-681(1998) [PubMed: 9700157] [Abstract] Cited for: IDENTIFICATION IN THE RETROMER COMPLEX.
[5]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[6]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-207, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U10400 Genomic DNA. Translation: AAB68947.1. BK006934 Genomic DNA. Translation: DAA06701.1.
PIR	S46793.
RefSeq	NP_011876.1. NM_001179142.1.
3D structure databases
ProteinModelPortal	P38759.
SMR	P38759. Positions 1-281.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-4205N.
IntAct	P38759. 8 interactions.
MINT	MINT-483798.
STRING	P38759.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YHR012W; YHR012W; YHR012W.
GeneID	856403.
KEGG	sce:YHR012W.
NMPDR	fig|4932.3.peg.3021.
Organism-specific databases
CYGD	YHR012w.
SGD	S000001054. VPS29.
Phylogenomic databases
eggNOG	fuNOG08134.
GeneTree	EFGT00050000003471.
HOGENOM	HBG317909.
OMA	THNVEAY.
OrthoDB	EOG4MGWHW.
Gene expression databases
ArrayExpress	P38759.
Genevestigator	P38759.
GermOnline	YHR012W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR024654. Calcineurin-like_PEstase_dom. IPR000979. Phosphodiesterase_MJ0936. [Graphical view]
KO	K07095.
PANTHER	PTHR11124. PTHR11124. 1 hit.
Pfam	PF12850. Metallophos_2. 1 hit. [Graphical view]
TIGRFAMs	TIGR00040. YfcE. 1 hit.
ProtoNet	Search...
Other
NextBio	981940.

Entry information

Entry name

VPS29_YEAST

Accession

Primary (citable) accession number: P38759
Secondary accession number(s): D3DKV7

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	February 1, 1995
Last modified:	December 14, 2011
This is version 94 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program