Putative oxidoreductase TDA3 - Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

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P38758 (TDA3_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified December 14, 2011. Version 84. History...

Clusters with 100%, 90%, 50% identity |

Documents (4) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Putative oxidoreductase TDA3
EC=1.-.-.-

Alternative name(s):
Batten disease protein 3
Topoisomerase I damage affected protein 3

Gene names

Name:	TDA3
Synonyms:	BTN3
Ordered Locus Names:	YHR009C

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	523 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Putative oxidoreductase that negatively regulates the retrieval of cargo from late endosomes to the Golgi. Regulates YIF1 and KEX2 localization. Required for fast DNA replication. Ref.10 Ref.12
Subunit structure	Interacts with BTN2. Ref.12
Subcellular location	Cytoplasm. Late endosome. Note: The recruitment from the cytoplasm to endosomal structures is facilitated by BTN2. Ref.3 Ref.5 Ref.12
Disruption phenotype	Leads to cell death when overexpressing the camptothecin mimetic TOP1-T(722)A mutant. Ref.11
Miscellaneous	Present with 5240 molecules/cell in log phase SD medium. Ref.6
Sequence similarities	Belongs to the TDA3 family.
Sequence caution	The sequence AAB68938.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

Ontologies

Keywords
Biological process	Transport
Cellular component	Cytoplasm Endosome
Molecular function	Oxidoreductase
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	retrograde transport, endosome to Golgi Inferred from mutant phenotype Ref.12. Source: SGD
Cellular component	late endosome Inferred from direct assay Ref.12. Source: SGD
Molecular function	oxidoreductase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct
ESS1	P22696	1	EBI-24443,EBI-6679
PRP40	P33203	1	EBI-24443,EBI-701
RSP5	P39940	1	EBI-24443,EBI-16219
SSM4	P40318	1	EBI-24443,EBI-18208
URN1	Q06525	1	EBI-24443,EBI-35138
VTS1	Q08831	1	EBI-24443,EBI-36342

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 523

523

Putative oxidoreductase TDA3

PRO_0000202886

Regions

Compositional bias

156 – 212

Ser-rich

Amino acid modifications

Modified residue

158

Phosphoserine Ref.9

Modified residue

159

Phosphoserine Ref.9

Modified residue

184

Phosphoserine Ref.9

Modified residue

200

Phosphoserine Ref.8 Ref.9

Modified residue

202

Phosphothreonine Ref.8 Ref.9

Modified residue

204

Phosphoserine Ref.8 Ref.9

Modified residue

206

Phosphoserine Ref.8 Ref.9

Modified residue

210

Phosphoserine Ref.9

Modified residue

212

Phosphoserine Ref.8 Ref.9

Modified residue

306

Phosphoserine Ref.7

Sequences

Sequence

Length

Mass (Da)

Tools

P38758 [UniParc].

Last modified May 16, 2006. Version 2.
Checksum: 7D02A4C731758B6D
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FASTA

523

57,614

        10         20         30         40         50         60 
MGEDFMHPPF QTYPSKNSEG KKHIVIVGGG IIGCCTAYYL TQHPSFSPST HHITIIESRR 

        70         80         90        100        110        120 
IAGGASGKAG GLLASWAFPH QIVPLSFQLH QELSDEYDGE NNWDYRRLTT VSLEADVREE 

       130        140        150        160        170        180 
VIENYERLSK KAYNLNVPPP KKRPGYISNK FNIGDSNSSL SSSGSSLKND SASNEEEGSD 

       190        200        210        220        230        240 
IHVSSSVPSL HSLTNERMRS HTNSASDLDS VSPVEQLRET NIHNPLPADL DWIRRELVND 

       250        260        270        280        290        300 
WSSLGGTDTT AQLHPYKFTH FILSKAMETG AVDLLLGKVV GLKCDEMDCV HSLKYLPSVV 

       310        320        330        340        350        360 
KNRRNSRGHA ENPDIKLGTI FNDENAKPIE INDIQQIVLS MGPWTSKILK DCPISGLRAH 

       370        380        390        400        410        420 
SVTIKPSEKT VSPYAILAEL KVNDREFFSP EMYARKDEVY VCGEGDTLVN IPESSDDVEV 

       430        440        450        460        470        480 
VSEKCDELYH YVSKLSPTLS KGHLLRKQAC FLPVLNVPTS SGPLIGETNV KDLYIASGHS 

       490        500        510        520 
CWGINNAPAT GKLMAEILLD GEATSAEISS LDPKLYFDAT ILS

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete nucleotide sequence of Saccharomyces cerevisiae chromosome VIII." Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Dover J., Du Z., Favello A., Fulton L., Gattung S., Geisel C., Kirsten J., Kucaba T., Hillier L.W., Jier M., Johnston L., Langston Y., Latreille P. Vaudin M. Science 265:2077-2082(1994) [PubMed: 8091229] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[3]	"The yeast nuclear pore complex: composition, architecture, and transport mechanism." Rout M.P., Aitchison J.D., Suprapto A., Hjertaas K., Zhao Y., Chait B.T. J. Cell Biol. 148:635-651(2000) [PubMed: 10684247] [Abstract] Cited for: SUBCELLULAR LOCATION, IDENTIFICATION BY MASS SPECTROMETRY.
[4]	"Sequencing and comparison of yeast species to identify genes and regulatory elements." Kellis M., Patterson N., Endrizzi M., Birren B.W., Lander E.S. Nature 423:241-254(2003) [PubMed: 12748633] [Abstract] Cited for: IDENTIFICATION OF PROBABLE INITIATION SITE.
[5]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed: 14562095] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[6]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[7]	"Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry." Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F. Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed: 17287358] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-306, MASS SPECTROMETRY.
[8]	"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H. Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed: 17563356] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-200; THR-202; SER-204; SER-206 AND SER-212, MASS SPECTROMETRY.
[9]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-158; SER-159; SER-184; SER-200; THR-202; SER-204; SER-206; SER-210 AND SER-212, MASS SPECTROMETRY.
[10]	"MRC1-dependent scaling of the budding yeast DNA replication timing program." Koren A., Soifer I., Barkai N. Genome Res. 20:781-790(2010) [PubMed: 20219942] [Abstract] Cited for: FUNCTION.
[11]	"Selective ploidy ablation, a high-throughput plasmid transfer protocol, identifies new genes affecting topoisomerase I-induced DNA damage." Reid R.J., Gonzalez-Barrera S., Sunjevaric I., Alvaro D., Ciccone S., Wagner M., Rothstein R. Genome Res. 21:477-486(2011) [PubMed: 21173034] [Abstract] Cited for: DISRUPTION PHENOTYPE.
[12]	"Btn3 is a negative regulator of Btn2-mediated endosomal protein trafficking and prion curing in yeast." Kanneganti V., Kama R., Gerst J.E. Mol. Biol. Cell 22:1648-1663(2011) [PubMed: 21441304] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH BTN2.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U10400 Genomic DNA. Translation: AAB68938.1. Different initiation. BK006934 Genomic DNA. Translation: DAA06698.1.
PIR	S46784.
RefSeq	NP_011873.1. NM_001179139.1.
3D structure databases
ProteinModelPortal	P38758.
SMR	P38758. Positions 21-63.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-4617N.
IntAct	P38758. 12 interactions.
MINT	MINT-479933.
STRING	P38758.
Proteomic databases
PeptideAtlas	P38758.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YHR009C; YHR009C; YHR009C.
GeneID	856400.
KEGG	sce:YHR009C.
NMPDR	fig\|4932.3.peg.3018.
Organism-specific databases
CYGD	YHR009c.
SGD	S000001051. TDA3.
Phylogenomic databases
eggNOG	fuNOG07931.
GeneTree	EFGT00050000004163.
HOGENOM	HBG603845.
OMA	GPLIGET.
OrthoDB	EOG4JMBZP.
Gene expression databases
ArrayExpress	P38758.
Genevestigator	P38758.
GermOnline	YHR009C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR006076. FAD-dep_OxRdtase. [Graphical view]
Pfam	PF01266. DAO. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	981931.

Entry information

Entry name

TDA3_YEAST

Accession

Primary (citable) accession number: P38758
Secondary accession number(s): D3DKV4

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	May 16, 2006
Last modified:	December 14, 2011
This is version 84 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome VIII

Yeast (Saccharomyces cerevisiae) chromosome VIII: entries and gene names

Uncharacterized protein families (UPF)

List of uncharacterized protein family (UPF) entries

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents