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P38757

- NEM1_YEAST

UniProt

P38757 - NEM1_YEAST

Protein

Nuclear envelope morphology protein 1

Gene

NEM1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 116 (01 Oct 2014)
      Sequence version 1 (01 Feb 1995)
      Previous versions | rss
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    Functioni

    Essential for the formation of a spherical nucleus and meiotic division. Catalytic component of the NEM1-SPO7 complex which acts as a phosphatase and dephosphorylates SMP2.2 Publications

    Catalytic activityi

    [a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

    GO - Molecular functioni

    1. phosphoprotein phosphatase activity Source: UniProtKB-KW
    2. protein binding Source: IntAct

    GO - Biological processi

    1. dephosphorylation Source: GOC
    2. negative regulation of phospholipid biosynthetic process Source: SGD
    3. nuclear envelope organization Source: SGD

    Keywords - Molecular functioni

    Hydrolase, Protein phosphatase

    Enzyme and pathway databases

    BioCyciYEAST:G3O-31069-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Nuclear envelope morphology protein 1 (EC:3.1.3.16)
    Gene namesi
    Name:NEM1
    Ordered Locus Names:YHR004C
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome VIII

    Organism-specific databases

    CYGDiYHR004c.
    SGDiS000001046. NEM1.

    Subcellular locationi

    Endoplasmic reticulum membrane 1 Publication; Single-pass membrane protein 1 Publication. Nucleus membrane 1 Publication; Single-pass membrane protein 1 Publication

    GO - Cellular componenti

    1. endoplasmic reticulum membrane Source: UniProtKB-SubCell
    2. integral component of membrane Source: SGD
    3. Nem1-Spo7 phosphatase complex Source: SGD
    4. nuclear membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Endoplasmic reticulum, Membrane, Nucleus

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi257 – 2571D → A: Abolishes phosphatase activity. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 446446Nuclear envelope morphology protein 1PRO_0000212579Add
    BLAST

    Proteomic databases

    MaxQBiP38757.
    PaxDbiP38757.

    Expressioni

    Gene expression databases

    GenevestigatoriP38757.

    Interactioni

    Subunit structurei

    Component of the NEM1-SPO7 complex.1 Publication

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    SPO7P184104EBI-24435,EBI-17857

    Protein-protein interaction databases

    BioGridi36429. 101 interactions.
    DIPiDIP-2440N.
    IntActiP38757. 3 interactions.
    MINTiMINT-511205.
    STRINGi4932.YHR004C.

    Structurei

    3D structure databases

    ProteinModelPortaliP38757.
    SMRiP38757. Positions 238-424.
    ModBaseiSearch...
    MobiDBiSearch...

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei87 – 10317HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini247 – 424178FCP1 homologyPROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the Dullard family.Curated
    Contains 1 FCP1 homology domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG5190.
    GeneTreeiENSGT00550000075053.
    HOGENOMiHOG000246647.
    KOiK17617.
    OMAiCANIVTI.
    OrthoDBiEOG7VDZ0W.

    Family and domain databases

    Gene3Di3.40.50.1000. 1 hit.
    InterProiIPR011948. Dullard_phosphatase.
    IPR023214. HAD-like_dom.
    IPR004274. NIF.
    [Graphical view]
    PfamiPF03031. NIF. 1 hit.
    [Graphical view]
    SMARTiSM00577. CPDc. 1 hit.
    [Graphical view]
    SUPFAMiSSF56784. SSF56784. 1 hit.
    TIGRFAMsiTIGR02251. HIF-SF_euk. 1 hit.
    PROSITEiPS50969. FCP1. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P38757-1 [UniParc]FASTAAdd to Basket

    « Hide

    MNALKYFSNH LITTKKQKKI NVEVTKNQDL LGPSKEVSNK YTSHSENDCV    50
    SEVDQQYDHS SSHLKESDQN QERKNSVPKK PKALRSILIE KIASILWALL 100
    LFLPYYLIIK PLMSLWFVFT FPLSVIERRV KHTDKRNRGS NASENELPVS 150
    SSNINDSSEK TNPKNCNLNT IPEAVEDDLN ASDEIILQRD NVKGSLLRAQ 200
    SVKSRPRSYS KSELSLSNHS SSNTVFGTKR MGRFLFPKKL IPKSVLNTQK 250
    KKKLVIDLDE TLIHSASRST THSNSSQGHL VEVKFGLSGI RTLYFIHKRP 300
    YCDLFLTKVS KWYDLIIFTA SMKEYADPVI DWLESSFPSS FSKRYYRSDC 350
    VLRDGVGYIK DLSIVKDSEE NGKGSSSSLD DVIIIDNSPV SYAMNVDNAI 400
    QVEGWISDPT DTDLLNLLPF LEAMRYSTDV RNILALKHGE KAFNIN 446
    Length:446
    Mass (Da):50,642
    Last modified:February 1, 1995 - v1
    Checksum:i4260748606E17062
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U10555 Genomic DNA. Translation: AAB68431.1.
    AY692950 Genomic DNA. Translation: AAT92969.1.
    BK006934 Genomic DNA. Translation: DAA06691.1.
    PIRiS46802.
    RefSeqiNP_011867.1. NM_001179134.1.

    Genome annotation databases

    EnsemblFungiiYHR004C; YHR004C; YHR004C.
    GeneIDi856393.
    KEGGisce:YHR004C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U10555 Genomic DNA. Translation: AAB68431.1 .
    AY692950 Genomic DNA. Translation: AAT92969.1 .
    BK006934 Genomic DNA. Translation: DAA06691.1 .
    PIRi S46802.
    RefSeqi NP_011867.1. NM_001179134.1.

    3D structure databases

    ProteinModelPortali P38757.
    SMRi P38757. Positions 238-424.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 36429. 101 interactions.
    DIPi DIP-2440N.
    IntActi P38757. 3 interactions.
    MINTi MINT-511205.
    STRINGi 4932.YHR004C.

    Proteomic databases

    MaxQBi P38757.
    PaxDbi P38757.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YHR004C ; YHR004C ; YHR004C .
    GeneIDi 856393.
    KEGGi sce:YHR004C.

    Organism-specific databases

    CYGDi YHR004c.
    SGDi S000001046. NEM1.

    Phylogenomic databases

    eggNOGi COG5190.
    GeneTreei ENSGT00550000075053.
    HOGENOMi HOG000246647.
    KOi K17617.
    OMAi CANIVTI.
    OrthoDBi EOG7VDZ0W.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-31069-MONOMER.

    Miscellaneous databases

    NextBioi 981911.

    Gene expression databases

    Genevestigatori P38757.

    Family and domain databases

    Gene3Di 3.40.50.1000. 1 hit.
    InterProi IPR011948. Dullard_phosphatase.
    IPR023214. HAD-like_dom.
    IPR004274. NIF.
    [Graphical view ]
    Pfami PF03031. NIF. 1 hit.
    [Graphical view ]
    SMARTi SM00577. CPDc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56784. SSF56784. 1 hit.
    TIGRFAMsi TIGR02251. HIF-SF_euk. 1 hit.
    PROSITEi PS50969. FCP1. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    2. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    3. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    4. "A novel complex of membrane proteins required for formation of a spherical nucleus."
      Siniossoglou S., Santos-Rosa H., Rappsilber J., Mann M., Hurt E.
      EMBO J. 17:6449-6464(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, IDENTIFICATION IN THE NEM1-SPO7 COMPLEX, SUBCELLULAR LOCATION.
    5. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    6. "The yeast lipin Smp2 couples phospholipid biosynthesis to nuclear membrane growth."
      Santos-Rosa H., Leung J., Grimsey N., Peak-Chew S., Siniossoglou S.
      EMBO J. 24:1931-1941(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION OF THE NEM1-SPO7 COMPLEX, MUTAGENESIS OF ASP-257.

    Entry informationi

    Entry nameiNEM1_YEAST
    AccessioniPrimary (citable) accession number: P38757
    Secondary accession number(s): D3DKU7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1995
    Last sequence update: February 1, 1995
    Last modified: October 1, 2014
    This is version 116 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 377 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome VIII
      Yeast (Saccharomyces cerevisiae) chromosome VIII: entries and gene names

    External Data

    Dasty 3