Nuclear envelope morphology protein 1 - Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

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P38757 (NEM1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified May 29, 2013. Version 106. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Nuclear envelope morphology protein 1
EC=3.1.3.16

Gene names

Name:	NEM1
Ordered Locus Names:	YHR004C

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces ›

Protein attributes

Sequence length	446 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Essential for the formation of a spherical nucleus and meiotic division. Catalytic component of the NEM1-SPO7 complex which acts as a phosphatase and dephosphorylates SMP2. Ref.4 Ref.6
Catalytic activity	A phosphoprotein + H₂O = a protein + phosphate.
Subunit structure	Component of the NEM1-SPO7 complex. Ref.4
Subcellular location	Endoplasmic reticulum membrane; Single-pass membrane protein. Nucleus membrane; Single-pass membrane protein Ref.4.
Miscellaneous	Present with 377 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the Dullard family. Contains 1 FCP1 homology domain.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum Membrane Nucleus
Domain	Transmembrane Transmembrane helix
Molecular function	Hydrolase Protein phosphatase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	dephosphorylation Inferred from electronic annotation. Source: GOC negative regulation of phospholipid biosynthetic process Inferred from genetic interaction Ref.6. Source: SGD nuclear envelope organization Inferred from mutant phenotype Ref.4. Source: SGD
Cellular_component	Nem1-Spo7 phosphatase complex Inferred from physical interaction Ref.4. Source: SGD endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from sequence model Ref.4. Source: SGD mitochondrion Inferred from direct assay PubMed 14576278 PubMed 16823961. Source: SGD nuclear membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	phosphoprotein phosphatase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
SPO7	P18410	4	EBI-24435,EBI-17857

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 446

446

Nuclear envelope morphology protein 1

PRO_0000212579

Regions

Transmembrane

87 – 103

Helical; Potential

Domain

247 – 424

178

FCP1 homology

Experimental info

Mutagenesis

257

D → A: Abolishes phosphatase activity. Ref.6

Sequences

Sequence

Length

Mass (Da)

Tools

P38757 [UniParc].

Last modified February 1, 1995. Version 1.
Checksum: 4260748606E17062
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FASTA

446

50,642

        10         20         30         40         50         60 
MNALKYFSNH LITTKKQKKI NVEVTKNQDL LGPSKEVSNK YTSHSENDCV SEVDQQYDHS 

        70         80         90        100        110        120 
SSHLKESDQN QERKNSVPKK PKALRSILIE KIASILWALL LFLPYYLIIK PLMSLWFVFT 

       130        140        150        160        170        180 
FPLSVIERRV KHTDKRNRGS NASENELPVS SSNINDSSEK TNPKNCNLNT IPEAVEDDLN 

       190        200        210        220        230        240 
ASDEIILQRD NVKGSLLRAQ SVKSRPRSYS KSELSLSNHS SSNTVFGTKR MGRFLFPKKL 

       250        260        270        280        290        300 
IPKSVLNTQK KKKLVIDLDE TLIHSASRST THSNSSQGHL VEVKFGLSGI RTLYFIHKRP 

       310        320        330        340        350        360 
YCDLFLTKVS KWYDLIIFTA SMKEYADPVI DWLESSFPSS FSKRYYRSDC VLRDGVGYIK 

       370        380        390        400        410        420 
DLSIVKDSEE NGKGSSSSLD DVIIIDNSPV SYAMNVDNAI QVEGWISDPT DTDLLNLLPF 

       430        440 
LEAMRYSTDV RNILALKHGE KAFNIN

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete nucleotide sequence of Saccharomyces cerevisiae chromosome VIII." Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Dover J., Du Z., Favello A., Fulton L., Gattung S., Geisel C., Kirsten J., Kucaba T., Hillier L.W., Jier M., Johnston L., Langston Y., Latreille P. Vaudin M. Science 265:2077-2082(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[3]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. LaBaer J. Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[4]	"A novel complex of membrane proteins required for formation of a spherical nucleus." Siniossoglou S., Santos-Rosa H., Rappsilber J., Mann M., Hurt E. EMBO J. 17:6449-6464(1998) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, IDENTIFICATION IN THE NEM1-SPO7 COMPLEX, SUBCELLULAR LOCATION.
[5]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[6]	"The yeast lipin Smp2 couples phospholipid biosynthesis to nuclear membrane growth." Santos-Rosa H., Leung J., Grimsey N., Peak-Chew S., Siniossoglou S. EMBO J. 24:1931-1941(2005) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION OF THE NEM1-SPO7 COMPLEX, MUTAGENESIS OF ASP-257.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U10555 Genomic DNA. Translation: AAB68431.1. AY692950 Genomic DNA. Translation: AAT92969.1. BK006934 Genomic DNA. Translation: DAA06691.1.
PIR	S46802.
RefSeq	NP_011867.1. NM_001179134.1.
3D structure databases
ProteinModelPortal	P38757.
SMR	P38757. Positions 238-424.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-2440N.
IntAct	P38757. 2 interactions.
MINT	MINT-511205.
STRING	4932.YHR004C.
Proteomic databases
PaxDb	P38757.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YHR004C; YHR004C; YHR004C.
GeneID	856393.
KEGG	sce:YHR004C.
Organism-specific databases
CYGD	YHR004c.
SGD	S000001046. NEM1.
Phylogenomic databases
eggNOG	COG5190.
GeneTree	ENSGT00550000075053.
HOGENOM	HOG000246647.
OMA	AIQVEGW.
OrthoDB	EOG4KH63R.
Enzyme and pathway databases
BioCyc	YEAST:G3O-31069-MONOMER.
Gene expression databases
Genevestigator	P38757.
GermOnline	YHR004C. Saccharomyces cerevisiae.
Family and domain databases
Gene3D	3.40.50.1000. 1 hit.
InterPro	IPR011948. Dullard_phosphatase. IPR023214. HAD-like_dom. IPR004274. NIF. [Graphical view]
Pfam	PF03031. NIF. 1 hit. [Graphical view]
SMART	SM00577. CPDc. 1 hit. [Graphical view]
SUPFAM	SSF56784. HAD-like_dom. 1 hit.
TIGRFAMs	TIGR02251. HIF-SF_euk. 1 hit.
PROSITE	PS50969. FCP1. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	981911.

Entry information

Entry name

NEM1_YEAST

Accession

Primary (citable) accession number: P38757
Secondary accession number(s): D3DKU7

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	February 1, 1995
Last modified:	May 29, 2013
This is version 106 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome VIII

Yeast (Saccharomyces cerevisiae) chromosome VIII: entries and gene names

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Regions

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents