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P38757

- NEM1_YEAST

UniProt

P38757 - NEM1_YEAST

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Protein

Nuclear envelope morphology protein 1

Gene
NEM1, YHR004C
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Essential for the formation of a spherical nucleus and meiotic division. Catalytic component of the NEM1-SPO7 complex which acts as a phosphatase and dephosphorylates SMP2.2 Publications

Catalytic activityi

[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

GO - Molecular functioni

  1. phosphoprotein phosphatase activity Source: UniProtKB-KW
  2. protein binding Source: IntAct

GO - Biological processi

  1. dephosphorylation Source: GOC
  2. negative regulation of phospholipid biosynthetic process Source: SGD
  3. nuclear envelope organization Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protein phosphatase

Enzyme and pathway databases

BioCyciYEAST:G3O-31069-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Nuclear envelope morphology protein 1 (EC:3.1.3.16)
Gene namesi
Name:NEM1
Ordered Locus Names:YHR004C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome VIII

Organism-specific databases

CYGDiYHR004c.
SGDiS000001046. NEM1.

Subcellular locationi

Endoplasmic reticulum membrane; Single-pass membrane protein. Nucleus membrane; Single-pass membrane protein 1 Publication

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei87 – 10317Helical; Reviewed predictionAdd
BLAST

GO - Cellular componenti

  1. endoplasmic reticulum membrane Source: UniProtKB-SubCell
  2. integral component of membrane Source: SGD
  3. Nem1-Spo7 phosphatase complex Source: SGD
  4. nuclear membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi257 – 2571D → A: Abolishes phosphatase activity. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 446446Nuclear envelope morphology protein 1PRO_0000212579Add
BLAST

Proteomic databases

MaxQBiP38757.
PaxDbiP38757.

Expressioni

Gene expression databases

GenevestigatoriP38757.

Interactioni

Subunit structurei

Component of the NEM1-SPO7 complex.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
SPO7P184104EBI-24435,EBI-17857

Protein-protein interaction databases

BioGridi36429. 101 interactions.
DIPiDIP-2440N.
IntActiP38757. 3 interactions.
MINTiMINT-511205.
STRINGi4932.YHR004C.

Structurei

3D structure databases

ProteinModelPortaliP38757.
SMRiP38757. Positions 238-424.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini247 – 424178FCP1 homologyAdd
BLAST

Sequence similaritiesi

Belongs to the Dullard family.

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG5190.
GeneTreeiENSGT00550000075053.
HOGENOMiHOG000246647.
KOiK17617.
OMAiCANIVTI.
OrthoDBiEOG7VDZ0W.

Family and domain databases

Gene3Di3.40.50.1000. 1 hit.
InterProiIPR011948. Dullard_phosphatase.
IPR023214. HAD-like_dom.
IPR004274. NIF.
[Graphical view]
PfamiPF03031. NIF. 1 hit.
[Graphical view]
SMARTiSM00577. CPDc. 1 hit.
[Graphical view]
SUPFAMiSSF56784. SSF56784. 1 hit.
TIGRFAMsiTIGR02251. HIF-SF_euk. 1 hit.
PROSITEiPS50969. FCP1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P38757-1 [UniParc]FASTAAdd to Basket

« Hide

MNALKYFSNH LITTKKQKKI NVEVTKNQDL LGPSKEVSNK YTSHSENDCV    50
SEVDQQYDHS SSHLKESDQN QERKNSVPKK PKALRSILIE KIASILWALL 100
LFLPYYLIIK PLMSLWFVFT FPLSVIERRV KHTDKRNRGS NASENELPVS 150
SSNINDSSEK TNPKNCNLNT IPEAVEDDLN ASDEIILQRD NVKGSLLRAQ 200
SVKSRPRSYS KSELSLSNHS SSNTVFGTKR MGRFLFPKKL IPKSVLNTQK 250
KKKLVIDLDE TLIHSASRST THSNSSQGHL VEVKFGLSGI RTLYFIHKRP 300
YCDLFLTKVS KWYDLIIFTA SMKEYADPVI DWLESSFPSS FSKRYYRSDC 350
VLRDGVGYIK DLSIVKDSEE NGKGSSSSLD DVIIIDNSPV SYAMNVDNAI 400
QVEGWISDPT DTDLLNLLPF LEAMRYSTDV RNILALKHGE KAFNIN 446
Length:446
Mass (Da):50,642
Last modified:February 1, 1995 - v1
Checksum:i4260748606E17062
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U10555 Genomic DNA. Translation: AAB68431.1.
AY692950 Genomic DNA. Translation: AAT92969.1.
BK006934 Genomic DNA. Translation: DAA06691.1.
PIRiS46802.
RefSeqiNP_011867.1. NM_001179134.1.

Genome annotation databases

EnsemblFungiiYHR004C; YHR004C; YHR004C.
GeneIDi856393.
KEGGisce:YHR004C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U10555 Genomic DNA. Translation: AAB68431.1 .
AY692950 Genomic DNA. Translation: AAT92969.1 .
BK006934 Genomic DNA. Translation: DAA06691.1 .
PIRi S46802.
RefSeqi NP_011867.1. NM_001179134.1.

3D structure databases

ProteinModelPortali P38757.
SMRi P38757. Positions 238-424.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 36429. 101 interactions.
DIPi DIP-2440N.
IntActi P38757. 3 interactions.
MINTi MINT-511205.
STRINGi 4932.YHR004C.

Proteomic databases

MaxQBi P38757.
PaxDbi P38757.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YHR004C ; YHR004C ; YHR004C .
GeneIDi 856393.
KEGGi sce:YHR004C.

Organism-specific databases

CYGDi YHR004c.
SGDi S000001046. NEM1.

Phylogenomic databases

eggNOGi COG5190.
GeneTreei ENSGT00550000075053.
HOGENOMi HOG000246647.
KOi K17617.
OMAi CANIVTI.
OrthoDBi EOG7VDZ0W.

Enzyme and pathway databases

BioCyci YEAST:G3O-31069-MONOMER.

Miscellaneous databases

NextBioi 981911.

Gene expression databases

Genevestigatori P38757.

Family and domain databases

Gene3Di 3.40.50.1000. 1 hit.
InterProi IPR011948. Dullard_phosphatase.
IPR023214. HAD-like_dom.
IPR004274. NIF.
[Graphical view ]
Pfami PF03031. NIF. 1 hit.
[Graphical view ]
SMARTi SM00577. CPDc. 1 hit.
[Graphical view ]
SUPFAMi SSF56784. SSF56784. 1 hit.
TIGRFAMsi TIGR02251. HIF-SF_euk. 1 hit.
PROSITEi PS50969. FCP1. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. "A novel complex of membrane proteins required for formation of a spherical nucleus."
    Siniossoglou S., Santos-Rosa H., Rappsilber J., Mann M., Hurt E.
    EMBO J. 17:6449-6464(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, IDENTIFICATION IN THE NEM1-SPO7 COMPLEX, SUBCELLULAR LOCATION.
  5. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  6. "The yeast lipin Smp2 couples phospholipid biosynthesis to nuclear membrane growth."
    Santos-Rosa H., Leung J., Grimsey N., Peak-Chew S., Siniossoglou S.
    EMBO J. 24:1931-1941(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION OF THE NEM1-SPO7 COMPLEX, MUTAGENESIS OF ASP-257.

Entry informationi

Entry nameiNEM1_YEAST
AccessioniPrimary (citable) accession number: P38757
Secondary accession number(s): D3DKU7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: June 11, 2014
This is version 115 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 377 molecules/cell in log phase SD medium.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome VIII
    Yeast (Saccharomyces cerevisiae) chromosome VIII: entries and gene names

External Data

Dasty 3

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