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Protein

Nuclear envelope morphology protein 1

Gene

NEM1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalytic component of the NEM1-SPO7 complex which acts as a phosphatase and dephosphorylates the phosphatidic acid phosphohydrolase PAH1 (PubMed:15889145). Essential for the formation of a spherical nucleus and meiotic division (PubMed:9822591). The NEM1-SPOo7 protein phosphatase is required for efficient mitophagy under prolonged respiration, as well as for reticulophagy and pexophagy (PubMed:29305265).3 Publications

Miscellaneous

Present with 377 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.1 Publication

GO - Molecular functioni

GO - Biological processi

  • negative regulation of phospholipid biosynthetic process Source: SGD
  • nuclear envelope organization Source: SGD
  • positive regulation of phosphatidate phosphatase activity Source: SGD
  • protein dephosphorylation Source: SGD

Keywordsi

Molecular functionHydrolase, Protein phosphatase

Enzyme and pathway databases

BioCyciYEAST:G3O-31069-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Nuclear envelope morphology protein 1 (EC:3.1.3.16)
Gene namesi
Name:NEM1
Ordered Locus Names:YHR004C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VIII

Organism-specific databases

EuPathDBiFungiDB:YHR004C
SGDiS000001046 NEM1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei87 – 103HelicalSequence analysisAdd BLAST17

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane, Nucleus

Pathology & Biotechi

Disruption phenotypei

Leads to inefficient mitochondrial sequestration and degradation, as well as to defective reticulophagy and pexophagy (PubMed:29305265).1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi257D → A: Abolishes phosphatase activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002125791 – 446Nuclear envelope morphology protein 1Add BLAST446

Proteomic databases

MaxQBiP38757
PaxDbiP38757
PRIDEiP38757

PTM databases

iPTMnetiP38757

Interactioni

Subunit structurei

Component of the NEM1-SPO7 complex.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
SPO7P184103EBI-24435,EBI-17857

Protein-protein interaction databases

BioGridi36429, 222 interactors
DIPiDIP-2440N
IntActiP38757, 4 interactors
MINTiP38757
STRINGi4932.YHR004C

Structurei

3D structure databases

ProteinModelPortaliP38757
SMRiP38757
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini247 – 424FCP1 homologyPROSITE-ProRule annotationAdd BLAST178

Sequence similaritiesi

Belongs to the Dullard family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

GeneTreeiENSGT00910000146475
HOGENOMiHOG000246647
InParanoidiP38757
KOiK17617
OMAiHTDANTI
OrthoDBiEOG092C5CYS

Family and domain databases

Gene3Di3.40.50.1000, 1 hit
InterProiView protein in InterPro
IPR011948 Dullard_phosphatase
IPR004274 FCP1_dom
IPR036412 HAD-like_sf
IPR023214 HAD_sf
PfamiView protein in Pfam
PF03031 NIF, 1 hit
SMARTiView protein in SMART
SM00577 CPDc, 1 hit
SUPFAMiSSF56784 SSF56784, 1 hit
TIGRFAMsiTIGR02251 HIF-SF_euk, 1 hit
PROSITEiView protein in PROSITE
PS50969 FCP1, 1 hit

Sequencei

Sequence statusi: Complete.

P38757-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNALKYFSNH LITTKKQKKI NVEVTKNQDL LGPSKEVSNK YTSHSENDCV
60 70 80 90 100
SEVDQQYDHS SSHLKESDQN QERKNSVPKK PKALRSILIE KIASILWALL
110 120 130 140 150
LFLPYYLIIK PLMSLWFVFT FPLSVIERRV KHTDKRNRGS NASENELPVS
160 170 180 190 200
SSNINDSSEK TNPKNCNLNT IPEAVEDDLN ASDEIILQRD NVKGSLLRAQ
210 220 230 240 250
SVKSRPRSYS KSELSLSNHS SSNTVFGTKR MGRFLFPKKL IPKSVLNTQK
260 270 280 290 300
KKKLVIDLDE TLIHSASRST THSNSSQGHL VEVKFGLSGI RTLYFIHKRP
310 320 330 340 350
YCDLFLTKVS KWYDLIIFTA SMKEYADPVI DWLESSFPSS FSKRYYRSDC
360 370 380 390 400
VLRDGVGYIK DLSIVKDSEE NGKGSSSSLD DVIIIDNSPV SYAMNVDNAI
410 420 430 440
QVEGWISDPT DTDLLNLLPF LEAMRYSTDV RNILALKHGE KAFNIN
Length:446
Mass (Da):50,642
Last modified:February 1, 1995 - v1
Checksum:i4260748606E17062
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U10555 Genomic DNA Translation: AAB68431.1
AY692950 Genomic DNA Translation: AAT92969.1
BK006934 Genomic DNA Translation: DAA06691.1
PIRiS46802
RefSeqiNP_011867.1, NM_001179134.1

Genome annotation databases

EnsemblFungiiYHR004C; YHR004C; YHR004C
GeneIDi856393
KEGGisce:YHR004C

Similar proteinsi

Entry informationi

Entry nameiNEM1_YEAST
AccessioniPrimary (citable) accession number: P38757
Secondary accession number(s): D3DKU7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: April 25, 2018
This is version 142 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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