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P38757 (NEM1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 113. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Nuclear envelope morphology protein 1

EC=3.1.3.16
Gene names
Name:NEM1
Ordered Locus Names:YHR004C
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length446 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Essential for the formation of a spherical nucleus and meiotic division. Catalytic component of the NEM1-SPO7 complex which acts as a phosphatase and dephosphorylates SMP2. Ref.4 Ref.6

Catalytic activity

[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

Subunit structure

Component of the NEM1-SPO7 complex. Ref.4

Subcellular location

Endoplasmic reticulum membrane; Single-pass membrane protein. Nucleus membrane; Single-pass membrane protein Ref.4.

Miscellaneous

Present with 377 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the Dullard family.

Contains 1 FCP1 homology domain.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

SPO7P184104EBI-24435,EBI-17857

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 446446Nuclear envelope morphology protein 1
PRO_0000212579

Regions

Transmembrane87 – 10317Helical; Potential
Domain247 – 424178FCP1 homology

Experimental info

Mutagenesis2571D → A: Abolishes phosphatase activity. Ref.6

Sequences

Sequence LengthMass (Da)Tools
P38757 [UniParc].

Last modified February 1, 1995. Version 1.
Checksum: 4260748606E17062

FASTA44650,642
        10         20         30         40         50         60 
MNALKYFSNH LITTKKQKKI NVEVTKNQDL LGPSKEVSNK YTSHSENDCV SEVDQQYDHS 

        70         80         90        100        110        120 
SSHLKESDQN QERKNSVPKK PKALRSILIE KIASILWALL LFLPYYLIIK PLMSLWFVFT 

       130        140        150        160        170        180 
FPLSVIERRV KHTDKRNRGS NASENELPVS SSNINDSSEK TNPKNCNLNT IPEAVEDDLN 

       190        200        210        220        230        240 
ASDEIILQRD NVKGSLLRAQ SVKSRPRSYS KSELSLSNHS SSNTVFGTKR MGRFLFPKKL 

       250        260        270        280        290        300 
IPKSVLNTQK KKKLVIDLDE TLIHSASRST THSNSSQGHL VEVKFGLSGI RTLYFIHKRP 

       310        320        330        340        350        360 
YCDLFLTKVS KWYDLIIFTA SMKEYADPVI DWLESSFPSS FSKRYYRSDC VLRDGVGYIK 

       370        380        390        400        410        420 
DLSIVKDSEE NGKGSSSSLD DVIIIDNSPV SYAMNVDNAI QVEGWISDPT DTDLLNLLPF 

       430        440 
LEAMRYSTDV RNILALKHGE KAFNIN 

« Hide

References

« Hide 'large scale' references
[1]"Complete nucleotide sequence of Saccharomyces cerevisiae chromosome VIII."
Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Dover J., Du Z., Favello A., Fulton L., Gattung S., Geisel C., Kirsten J., Kucaba T., Hillier L.W., Jier M., Johnston L., Langston Y., Latreille P. expand/collapse author list , Louis E.J., Macri C., Mardis E., Menezes S., Mouser L., Nhan M., Rifkin L., Riles L., St Peter H., Trevaskis E., Vaughan K., Vignati D., Wilcox L., Wohldman P., Waterston R., Wilson R., Vaudin M.
Science 265:2077-2082(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[2]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[3]"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae."
Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. expand/collapse author list , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J.
Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[4]"A novel complex of membrane proteins required for formation of a spherical nucleus."
Siniossoglou S., Santos-Rosa H., Rappsilber J., Mann M., Hurt E.
EMBO J. 17:6449-6464(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, IDENTIFICATION IN THE NEM1-SPO7 COMPLEX, SUBCELLULAR LOCATION.
[5]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[6]"The yeast lipin Smp2 couples phospholipid biosynthesis to nuclear membrane growth."
Santos-Rosa H., Leung J., Grimsey N., Peak-Chew S., Siniossoglou S.
EMBO J. 24:1931-1941(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION OF THE NEM1-SPO7 COMPLEX, MUTAGENESIS OF ASP-257.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U10555 Genomic DNA. Translation: AAB68431.1.
AY692950 Genomic DNA. Translation: AAT92969.1.
BK006934 Genomic DNA. Translation: DAA06691.1.
PIRS46802.
RefSeqNP_011867.1. NM_001179134.1.

3D structure databases

ProteinModelPortalP38757.
SMRP38757. Positions 238-424.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid36429. 101 interactions.
DIPDIP-2440N.
IntActP38757. 3 interactions.
MINTMINT-511205.
STRING4932.YHR004C.

Proteomic databases

PaxDbP38757.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYHR004C; YHR004C; YHR004C.
GeneID856393.
KEGGsce:YHR004C.

Organism-specific databases

CYGDYHR004c.
SGDS000001046. NEM1.

Phylogenomic databases

eggNOGCOG5190.
GeneTreeENSGT00550000075053.
HOGENOMHOG000246647.
KOK17617.
OMAAIQVEGW.
OrthoDBEOG7VDZ0W.

Enzyme and pathway databases

BioCycYEAST:G3O-31069-MONOMER.

Gene expression databases

GenevestigatorP38757.

Family and domain databases

Gene3D3.40.50.1000. 1 hit.
InterProIPR011948. Dullard_phosphatase.
IPR023214. HAD-like_dom.
IPR004274. NIF.
[Graphical view]
PfamPF03031. NIF. 1 hit.
[Graphical view]
SMARTSM00577. CPDc. 1 hit.
[Graphical view]
SUPFAMSSF56784. SSF56784. 1 hit.
TIGRFAMsTIGR02251. HIF-SF_euk. 1 hit.
PROSITEPS50969. FCP1. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio981911.

Entry information

Entry nameNEM1_YEAST
AccessionPrimary (citable) accession number: P38757
Secondary accession number(s): D3DKU7
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: February 19, 2014
This is version 113 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome VIII

Yeast (Saccharomyces cerevisiae) chromosome VIII: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families