Reviewed, UniProtKB/Swiss-Prot P38757 (NEM1_YEAST)

Names and origin

Protein names

Recommended name:
    Nuclear envelope morphology protein 1
    EC=3.1.3.16

Gene names

Name:	NEM1
Ordered Locus Names:	YHR004C

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	446 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Essential for the formation of a spherical nucleus and meiotic division. Catalytic component of the NEM1-SPO7 complex which acts as a phosphatase and dephosphorylates SMP2. Ref.3 Ref.5
Catalytic activity	A phosphoprotein + H2O = a protein + phosphate.
Subunit structure	Component of the NEM1-SPO7 complex.
Subcellular location	Endoplasmic reticulum membrane; Single-pass membrane protein. Nucleus membrane; Single-pass membrane protein.
Miscellaneous	Present with 377 molecules/cell in log phase SD medium. Ref.4
Sequence similarities	Belongs to the Dullard family. Contains 1 FCP1 homology domain.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum Membrane Nucleus
Domain	Transmembrane
Molecular function	Hydrolase Protein phosphatase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	ascospore formation Ref.3 Inferred from mutant phenotype. Source: SGD nuclear envelope organization Ref.3 Inferred from mutant phenotype. Source: SGD regulation of lipid biosynthetic process Ref.5 Inferred from genetic interaction. Source: SGD
Cellular component	endoplasmic reticulum Inferred from electronic annotation. Source: UniProtKB-KW integral to membrane Ref.3 Inferred from direct assay. Source: SGD mitochondrion Inferred from direct assay. Source: SGD nuclear envelope-endoplasmic reticulum network Ref.3 Inferred from direct assay. Source: SGD nucleus Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	phosphoprotein phosphatase activity Ref.5 Inferred from electronic annotation. Source: UniProtKB-KW protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
SMB1	P40018	1	EBI-24435,EBI-432

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 446	446	Nuclear envelope morphology protein 1		PRO_0000212579
Regions
	Transmembrane	87 – 103	17	Potential
	Domain	247 – 424	178	FCP1 homology
Experimental info
	Mutagenesis	257	1	D → A: Abolishes phosphatase activity. Ref.5

Sequences

	Sequence		Length	Mass (Da)
	P38757-1 [UniParc]. Last modified February 1, 1995. Version 1. Checksum: 4260748606E17062	FASTA	446	50,642
	10 20 30 40 50 60 MNALKYFSNH LITTKKQKKI NVEVTKNQDL LGPSKEVSNK YTSHSENDCV SEVDQQYDHS 70 80 90 100 110 120 SSHLKESDQN QERKNSVPKK PKALRSILIE KIASILWALL LFLPYYLIIK PLMSLWFVFT 130 140 150 160 170 180 FPLSVIERRV KHTDKRNRGS NASENELPVS SSNINDSSEK TNPKNCNLNT IPEAVEDDLN 190 200 210 220 230 240 ASDEIILQRD NVKGSLLRAQ SVKSRPRSYS KSELSLSNHS SSNTVFGTKR MGRFLFPKKL 250 260 270 280 290 300 IPKSVLNTQK KKKLVIDLDE TLIHSASRST THSNSSQGHL VEVKFGLSGI RTLYFIHKRP 310 320 330 340 350 360 YCDLFLTKVS KWYDLIIFTA SMKEYADPVI DWLESSFPSS FSKRYYRSDC VLRDGVGYIK 370 380 390 400 410 420 DLSIVKDSEE NGKGSSSSLD DVIIIDNSPV SYAMNVDNAI QVEGWISDPT DTDLLNLLPF 430 440 LEAMRYSTDV RNILALKHGE KAFNIN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete nucleotide sequence of Saccharomyces cerevisiae chromosome VIII." Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Dover J., Du Z., Favello A., Fulton L., Gattung S., Geisel C., Kirsten J., Kucaba T., Hillier L.W., Jier M., Johnston L., Langston Y., Latreille P. , Louis E.J., Macri C., Mardis E., Menezes S., Mouser L., Nhan M., Rifkin L., Riles L., St Peter H., Trevaskis E., Vaughan K., Vignati D., Wilcox L., Wohldman P., Waterston R., Wilson R., Vaudin M. Science 265:2077-2082(1994) [PubMed: 8091229] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed: 17322287] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[3]	"A novel complex of membrane proteins required for formation of a spherical nucleus." Siniossoglou S., Santos-Rosa H., Rappsilber J., Mann M., Hurt E. EMBO J. 17:6449-6464(1998) [PubMed: 9822591] [Abstract] Cited for: FUNCTION, IDENTIFICATION IN THE NEM1-SPO7 COMPLEX, SUBCELLULAR LOCATION.
[4]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[5]	"The yeast lipin Smp2 couples phospholipid biosynthesis to nuclear membrane growth." Santos-Rosa H., Leung J., Grimsey N., Peak-Chew S., Siniossoglou S. EMBO J. 24:1931-1941(2005) [PubMed: 15889145] [Abstract] Cited for: FUNCTION OF THE NEM1-SPO7 COMPLEX, MUTAGENESIS OF ASP-257.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U10555 Genomic DNA. Translation: AAB68431.1. AY692950 Genomic DNA. Translation: AAT92969.1.
PIR	S46802.
RefSeq	NP_011867.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:2440N.
IntAct	P38757. 3 interactions.
STRING	P38757.
Genome annotation databases
Ensembl	YHR004C; YHR004C; YHR004C; Saccharomyces cerevisiae. [Genome view]
GeneID	856393.
KEGG	sce:YHR004C.
NMPDR	fig|4932.3.peg.3011.
Organism-specific databases
CYGD	YHR004c.
SGD	S000001046. NEM1.
Phylogenomic databases
HOGENOM	P38757.
OMA	IQVEGWI
OrthoDB	EOG9T79KP
Enzyme and pathway databases
BRENDA	3.1.3.16. 250.
Gene expression databases
ArrayExpress	P38757.
Genevestigator	P38757.
GermOnline	YHR004C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR011948. Dullard. IPR004274. NIF. [Graphical view]
Pfam	PF03031. NIF. 1 hit. [Graphical view]
SMART	SM00577. CPDc. 1 hit. [Graphical view]
TIGRFAMs	TIGR02251. HIF-SF_euk. 1 hit.
PROSITE	PS50969. FCP1. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	981911.

Entry information

Entry name

NEM1_YEAST

Accession

Primary (citable) accession number: P38757

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	February 1, 1995
Last modified:	November 24, 2009
This is version 73 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)