Reviewed, UniProtKB/Swiss-Prot P38754 (RL14B_YEAST)

Names and origin

Protein names

Recommended name:
    60S ribosomal protein L14-B

Gene names

Name:	RPL14B
Ordered Locus Names:	YHL001W

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	138 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Subunit structure	Component of the large ribosomal subunit. Mature ribosomes consist of a small (40S) and a large (60S) subunit. The 40S subunit contains 32 different proteins (encoded by 56 genes) and 1 molecule of RNA (18S). The 60S subunit contains 46 different proteins (encoded by 81 genes) and 3 molecules of RNA (25S, 5.8S and 5S). Ref.2
Subcellular location	Cytoplasm Ref.3.
Post-translational modification	N-terminally acetylated by acetyltransferase NatA By similarity.
Miscellaneous	Present with 35100 molecules/cell in log phase SD medium. Ref.4 There are 2 genes for L14 in yeast.
Sequence similarities	Belongs to the ribosomal protein L14e family.

Ontologies

Keywords
Cellular component	Cytoplasm
Molecular function	Ribonucleoprotein Ribosomal protein
PTM	Acetylation Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	translation Ref.2 Traceable author statement. Source: SGD
Cellular component	cytosolic large ribosomal subunit Ref.2 Traceable author statement. Source: SGD
Molecular function	RNA binding Inferred from direct assay. Source: SGD structural constituent of ribosome Ref.2 Traceable author statement. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 138	137	60S ribosomal protein L14-B		PRO_0000132044
Amino acid modifications
	Modified residue	2	1	N-acetylserine By similarity
	Modified residue	10	1	Phosphoserine Ref.5

Sequences

	Sequence		Length	Mass (Da)
	P38754-1 [UniParc]. Last modified February 1, 1995. Version 1. Checksum: F8F6359DC29395BC	FASTA	138	15,153
	10 20 30 40 50 60 MSTDSIVKAS NWRLVEVGRV VLIKKGQSAG KLAAIVEIID QKKVLIDGPK AGVPRQAINL 70 80 90 100 110 120 GQVVLTPLTF ALPRGARTAT VSKKWAAAGV CEKWAASSWA KKIAQRERRA ALTDFERFQV 130 MVLRKQKRYT VKKALAKA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete nucleotide sequence of Saccharomyces cerevisiae chromosome VIII." Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Dover J., Du Z., Favello A., Fulton L., Gattung S., Geisel C., Kirsten J., Kucaba T., Hillier L.W., Jier M., Johnston L., Langston Y., Latreille P. , Louis E.J., Macri C., Mardis E., Menezes S., Mouser L., Nhan M., Rifkin L., Riles L., St Peter H., Trevaskis E., Vaughan K., Vignati D., Wilcox L., Wohldman P., Waterston R., Wilson R., Vaudin M. Science 265:2077-2082(1994) [PubMed: 8091229] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	"The list of cytoplasmic ribosomal proteins of Saccharomyces cerevisiae." Planta R.J., Mager W.H. Yeast 14:471-477(1998) [PubMed: 9559554] [Abstract] Cited for: NOMENCLATURE, SUBUNIT.
[3]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed: 14562095] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[4]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[5]	"Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry." Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F. Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed: 17287358] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-10, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U10555 Genomic DNA. Translation: AAB68426.1.
PIR	S46797.
RefSeq	NP_011862.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-5568N.
IntAct	P38754. 19 interactions.
STRING	P38754.
Proteomic databases
PeptideAtlas	P38754.
PRIDE	P38754.
Genome annotation databases
Ensembl	YHL001W; YHL001W; YHL001W; Saccharomyces cerevisiae. [Genome view]
GeneID	856388.
KEGG	sce:YHL001W.
Organism-specific databases
CYGD	YHL001w.
SGD	S000000993. RPL14B.
Phylogenomic databases
HOGENOM	HBG499844.
OMA	QRAIRAK.
OrthoDB	EOG9PCC8W.
Gene expression databases
ArrayExpress	P38754.
Genevestigator	P38754.
GermOnline	YHL001W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR002784. Ribosomal_L14e. [Graphical view]
Pfam	PF01929. Ribosomal_L14e. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	981896.

Entry information

Entry name

RL14B_YEAST

Accession

Primary (citable) accession number: P38754

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	February 1, 1995
Last modified:	December 15, 2009
This is version 70 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)