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P38753

- HSE1_YEAST

UniProt

P38753 - HSE1_YEAST

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Protein

Class E vacuolar protein-sorting machinery protein HSE1

Gene

HSE1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Component of the ESCRT-0 complex which is the sorting receptor for ubiquitinated cargo proteins at the multivesicular body (MVB) and recruits ESCRT-I to the MVB outer membrane.4 Publications

GO - Molecular functioni

  1. ubiquitin binding Source: SGD

GO - Biological processi

  1. late endosome to vacuole transport Source: SGD
  2. protein targeting to vacuole Source: SGD
Complete GO annotation...

Keywords - Biological processi

Protein transport, Transport

Enzyme and pathway databases

BioCyciYEAST:G3O-31026-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Class E vacuolar protein-sorting machinery protein HSE1
Gene namesi
Name:HSE1
Ordered Locus Names:YHL002W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome VIII

Organism-specific databases

CYGDiYHL002w.
SGDiS000000994. HSE1.

Subcellular locationi

Endosome membrane 2 Publications; Peripheral membrane protein 2 Publications; Cytoplasmic side 2 Publications

GO - Cellular componenti

  1. endosome Source: SGD
  2. ESCRT-0 complex Source: SGD
  3. membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Endosome, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed1 Publication
Chaini2 – 452451Class E vacuolar protein-sorting machinery protein HSE1PRO_0000202885Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylserine1 Publication
Modified residuei162 – 1621Phosphoserine3 Publications

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiP38753.
PaxDbiP38753.

Expressioni

Gene expression databases

GenevestigatoriP38753.

Interactioni

Subunit structurei

Component of the ESCRT-0 complex composed of HSE1 and VPS27. Interacts with the ESCRT-I subunit VPS23, the UBP7 deubiquitinase and the E3 ligase RSP5.3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
ACF2Q121682EBI-1382,EBI-32973
AIM21P405634EBI-1382,EBI-25376
DOA1P360373EBI-1382,EBI-6017
STP22P256043EBI-1382,EBI-411625
UBBP0CG532EBI-1382,EBI-5333021From a different organism.
UBP7P404534EBI-1382,EBI-19857
VPS27P4034310EBI-1382,EBI-20380

Protein-protein interaction databases

BioGridi36423. 98 interactions.
DIPiDIP-1742N.
IntActiP38753. 44 interactions.
MINTiMINT-386551.
STRINGi4932.YHL002W.

Structurei

Secondary structure

1
452
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi289 – 2946
Helixi296 – 31015
Helixi316 – 3194
Helixi322 – 33110
Helixi332 – 3343
Helixi335 – 37137
Turni372 – 3743

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2PJWX-ray3.01H288-375[»]
DisProtiDP00635.
ProteinModelPortaliP38753.
SMRiP38753. Positions 14-127, 224-271, 288-375.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP38753.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini15 – 145131VHSPROSITE-ProRule annotationAdd
BLAST
Repeati162 – 18120UIMAdd
BLAST
Domaini217 – 27660SH3PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the STAM family.Curated
Contains 1 SH3 domain.PROSITE-ProRule annotation
Contains 1 UIM (ubiquitin-interacting motif) repeat.PROSITE-ProRule annotation
Contains 1 VHS domain.PROSITE-ProRule annotation

Keywords - Domaini

SH3 domain

Phylogenomic databases

eggNOGiNOG309509.
GeneTreeiENSGT00700000104139.
HOGENOMiHOG000094361.
InParanoidiP38753.
KOiK04705.
OMAiPEPEQAY.
OrthoDBiEOG75MW70.

Family and domain databases

Gene3Di1.25.40.90. 1 hit.
InterProiIPR008942. ENTH_VHS.
IPR001452. SH3_domain.
IPR003903. Ubiquitin-int_motif.
IPR002014. VHS.
IPR018205. VHS_subgr.
[Graphical view]
PfamiPF00018. SH3_1. 1 hit.
PF00790. VHS. 1 hit.
[Graphical view]
PRINTSiPR00452. SH3DOMAIN.
SMARTiSM00326. SH3. 1 hit.
SM00726. UIM. 1 hit.
SM00288. VHS. 1 hit.
[Graphical view]
SUPFAMiSSF48464. SSF48464. 1 hit.
SSF50044. SSF50044. 1 hit.
PROSITEiPS50002. SH3. 1 hit.
PS50330. UIM. 1 hit.
PS50179. VHS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P38753-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSSSAIKIRN ALLKATDPKL RSDNWQYILD VCDLVKEDPE DNGQEVMSLI
60 70 80 90 100
EKRLEQQDAN VILRTLSLTV SLAENCGSRL RQEISSKNFT SLLYALIESH
110 120 130 140 150
SVHITLKKAV TDVVKQLSDS FKDDPSLRAM GDLYDKIKRK APYLVQPNVP
160 170 180 190 200
EKHNMSTQAD NSDDEELQKA LKMSLFEYEK QKKLQEQEKE SAEVLPQQQQ
210 220 230 240 250
QHQQQNQAPA HKIPAQTVVR RVRALYDLTT NEPDELSFRK GDVITVLEQV
260 270 280 290 300
YRDWWKGALR GNMGIFPLNY VTPIVEPSKE EIEKEKNKEA IVFSQKTTID
310 320 330 340 350
QLHNSLNAAS KTGNSNEVLQ DPHIGDMYGS VTPLRPQVTR MLGKYAKEKE
360 370 380 390 400
DMLSLRQVLA NAERSYNQLM DRAANAHISP PVPGPALYAG MTHANNTPVM
410 420 430 440 450
PPQRQSYQSN EYSPYPSNLP IQHPTNSANN TPQYGYDLGY SVVSQPPPGY

EQ
Length:452
Mass (Da):51,161
Last modified:February 1, 1995 - v1
Checksum:i026267836BBADF69
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U10555 Genomic DNA. Translation: AAB68427.1.
BK006934 Genomic DNA. Translation: DAA06685.1.
PIRiS46798.
RefSeqiNP_011861.1. NM_001179082.1.

Genome annotation databases

EnsemblFungiiYHL002W; YHL002W; YHL002W.
GeneIDi856387.
KEGGisce:YHL002W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U10555 Genomic DNA. Translation: AAB68427.1 .
BK006934 Genomic DNA. Translation: DAA06685.1 .
PIRi S46798.
RefSeqi NP_011861.1. NM_001179082.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
2PJW X-ray 3.01 H 288-375 [» ]
DisProti DP00635.
ProteinModelPortali P38753.
SMRi P38753. Positions 14-127, 224-271, 288-375.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 36423. 98 interactions.
DIPi DIP-1742N.
IntActi P38753. 44 interactions.
MINTi MINT-386551.
STRINGi 4932.YHL002W.

Proteomic databases

MaxQBi P38753.
PaxDbi P38753.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YHL002W ; YHL002W ; YHL002W .
GeneIDi 856387.
KEGGi sce:YHL002W.

Organism-specific databases

CYGDi YHL002w.
SGDi S000000994. HSE1.

Phylogenomic databases

eggNOGi NOG309509.
GeneTreei ENSGT00700000104139.
HOGENOMi HOG000094361.
InParanoidi P38753.
KOi K04705.
OMAi PEPEQAY.
OrthoDBi EOG75MW70.

Enzyme and pathway databases

BioCyci YEAST:G3O-31026-MONOMER.

Miscellaneous databases

EvolutionaryTracei P38753.
NextBioi 981893.

Gene expression databases

Genevestigatori P38753.

Family and domain databases

Gene3Di 1.25.40.90. 1 hit.
InterProi IPR008942. ENTH_VHS.
IPR001452. SH3_domain.
IPR003903. Ubiquitin-int_motif.
IPR002014. VHS.
IPR018205. VHS_subgr.
[Graphical view ]
Pfami PF00018. SH3_1. 1 hit.
PF00790. VHS. 1 hit.
[Graphical view ]
PRINTSi PR00452. SH3DOMAIN.
SMARTi SM00326. SH3. 1 hit.
SM00726. UIM. 1 hit.
SM00288. VHS. 1 hit.
[Graphical view ]
SUPFAMi SSF48464. SSF48464. 1 hit.
SSF50044. SSF50044. 1 hit.
PROSITEi PS50002. SH3. 1 hit.
PS50330. UIM. 1 hit.
PS50179. VHS. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. "The Vps27p-Hse1p complex binds ubiquitin and mediates endosomal protein sorting."
    Bilodeau P.S., Urbanowski J.L., Winistorfer S.C., Piper R.C.
    Nat. Cell Biol. 4:534-539(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION IN THE ESCRT-0 COMPLEX, FUNCTION OF THE ESCRT-0 COMPLEX, SUBCELLULAR LOCATION.
  4. "Vps27-Hse1 and ESCRT-I complexes cooperate to increase efficiency of sorting ubiquitinated proteins at the endosome."
    Bilodeau P.S., Winistorfer S.C., Kearney W.R., Robertson A.D., Piper R.C.
    J. Cell Biol. 163:237-243(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION OF THE ESCRT-0 COMPLEX.
  5. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  7. "Protein-protein interactions of ESCRT complexes in the yeast Saccharomyces cerevisiae."
    Bowers K., Lottridge J., Helliwell S.B., Goldthwaite L.M., Luzio J.P., Stevens T.H.
    Traffic 5:194-210(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH RSP5; VPS23 AND VPS27, FUNCTION OF THE ESCRT-0 COMPLEX.
  8. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
    Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
    J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-162, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: ADR376.
  9. "Hse1, a component of the yeast Hrs-STAM ubiquitin-sorting complex, associates with ubiquitin peptidases and a ligase to control sorting efficiency into multivesicular bodies."
    Ren J., Kee Y., Huibregtse J.M., Piper R.C.
    Mol. Biol. Cell 18:324-335(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH RSP5 AND UBP7.
  10. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-162, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  11. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-162, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  12. Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT SER-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiHSE1_YEAST
AccessioniPrimary (citable) accession number: P38753
Secondary accession number(s): D3DKR2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: October 29, 2014
This is version 128 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 358 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome VIII
    Yeast (Saccharomyces cerevisiae) chromosome VIII: entries and gene names

External Data

Dasty 3