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P38753

- HSE1_YEAST

UniProt

P38753 - HSE1_YEAST

Protein

Class E vacuolar protein-sorting machinery protein HSE1

Gene

HSE1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 127 (01 Oct 2014)
      Sequence version 1 (01 Feb 1995)
      Previous versions | rss
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    Functioni

    Component of the ESCRT-0 complex which is the sorting receptor for ubiquitinated cargo proteins at the multivesicular body (MVB) and recruits ESCRT-I to the MVB outer membrane.4 Publications

    GO - Molecular functioni

    1. protein binding Source: IntAct
    2. ubiquitin binding Source: SGD

    GO - Biological processi

    1. late endosome to vacuole transport Source: SGD
    2. protein targeting to vacuole Source: SGD

    Keywords - Biological processi

    Protein transport, Transport

    Enzyme and pathway databases

    BioCyciYEAST:G3O-31026-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Class E vacuolar protein-sorting machinery protein HSE1
    Gene namesi
    Name:HSE1
    Ordered Locus Names:YHL002W
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome VIII

    Organism-specific databases

    CYGDiYHL002w.
    SGDiS000000994. HSE1.

    Subcellular locationi

    Endosome membrane 2 Publications; Peripheral membrane protein 2 Publications; Cytoplasmic side 2 Publications

    GO - Cellular componenti

    1. endosome Source: SGD
    2. endosome membrane Source: UniProtKB-SubCell
    3. ESCRT-0 complex Source: SGD

    Keywords - Cellular componenti

    Endosome, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11Removed1 Publication
    Chaini2 – 452451Class E vacuolar protein-sorting machinery protein HSE1PRO_0000202885Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21N-acetylserine1 Publication
    Modified residuei162 – 1621Phosphoserine3 Publications

    Keywords - PTMi

    Acetylation, Phosphoprotein

    Proteomic databases

    MaxQBiP38753.
    PaxDbiP38753.

    Expressioni

    Gene expression databases

    GenevestigatoriP38753.

    Interactioni

    Subunit structurei

    Component of the ESCRT-0 complex composed of HSE1 and VPS27. Interacts with the ESCRT-I subunit VPS23, the UBP7 deubiquitinase and the E3 ligase RSP5.3 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    ACF2Q121682EBI-1382,EBI-32973
    AIM21P405634EBI-1382,EBI-25376
    DOA1P360373EBI-1382,EBI-6017
    STP22P256043EBI-1382,EBI-411625
    UBBP0CG532EBI-1382,EBI-5333021From a different organism.
    UBP7P404534EBI-1382,EBI-19857
    VPS27P4034310EBI-1382,EBI-20380

    Protein-protein interaction databases

    BioGridi36423. 97 interactions.
    DIPiDIP-1742N.
    IntActiP38753. 44 interactions.
    MINTiMINT-386551.
    STRINGi4932.YHL002W.

    Structurei

    Secondary structure

    1
    452
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi289 – 2946
    Helixi296 – 31015
    Helixi316 – 3194
    Helixi322 – 33110
    Helixi332 – 3343
    Helixi335 – 37137
    Turni372 – 3743

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2PJWX-ray3.01H288-375[»]
    DisProtiDP00635.
    ProteinModelPortaliP38753.
    SMRiP38753. Positions 14-127, 224-271, 288-375.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP38753.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini15 – 145131VHSPROSITE-ProRule annotationAdd
    BLAST
    Repeati162 – 18120UIMAdd
    BLAST
    Domaini217 – 27660SH3PROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the STAM family.Curated
    Contains 1 SH3 domain.PROSITE-ProRule annotation
    Contains 1 UIM (ubiquitin-interacting motif) repeat.PROSITE-ProRule annotation
    Contains 1 VHS domain.PROSITE-ProRule annotation

    Keywords - Domaini

    SH3 domain

    Phylogenomic databases

    eggNOGiNOG309509.
    GeneTreeiENSGT00700000104139.
    HOGENOMiHOG000094361.
    KOiK04705.
    OMAiPEPEQAY.
    OrthoDBiEOG75MW70.

    Family and domain databases

    Gene3Di1.25.40.90. 1 hit.
    InterProiIPR008942. ENTH_VHS.
    IPR001452. SH3_domain.
    IPR003903. Ubiquitin-int_motif.
    IPR002014. VHS.
    IPR018205. VHS_subgr.
    [Graphical view]
    PfamiPF00018. SH3_1. 1 hit.
    PF00790. VHS. 1 hit.
    [Graphical view]
    PRINTSiPR00452. SH3DOMAIN.
    SMARTiSM00326. SH3. 1 hit.
    SM00726. UIM. 1 hit.
    SM00288. VHS. 1 hit.
    [Graphical view]
    SUPFAMiSSF48464. SSF48464. 1 hit.
    SSF50044. SSF50044. 1 hit.
    PROSITEiPS50002. SH3. 1 hit.
    PS50330. UIM. 1 hit.
    PS50179. VHS. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P38753-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSSSAIKIRN ALLKATDPKL RSDNWQYILD VCDLVKEDPE DNGQEVMSLI    50
    EKRLEQQDAN VILRTLSLTV SLAENCGSRL RQEISSKNFT SLLYALIESH 100
    SVHITLKKAV TDVVKQLSDS FKDDPSLRAM GDLYDKIKRK APYLVQPNVP 150
    EKHNMSTQAD NSDDEELQKA LKMSLFEYEK QKKLQEQEKE SAEVLPQQQQ 200
    QHQQQNQAPA HKIPAQTVVR RVRALYDLTT NEPDELSFRK GDVITVLEQV 250
    YRDWWKGALR GNMGIFPLNY VTPIVEPSKE EIEKEKNKEA IVFSQKTTID 300
    QLHNSLNAAS KTGNSNEVLQ DPHIGDMYGS VTPLRPQVTR MLGKYAKEKE 350
    DMLSLRQVLA NAERSYNQLM DRAANAHISP PVPGPALYAG MTHANNTPVM 400
    PPQRQSYQSN EYSPYPSNLP IQHPTNSANN TPQYGYDLGY SVVSQPPPGY 450
    EQ 452
    Length:452
    Mass (Da):51,161
    Last modified:February 1, 1995 - v1
    Checksum:i026267836BBADF69
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U10555 Genomic DNA. Translation: AAB68427.1.
    BK006934 Genomic DNA. Translation: DAA06685.1.
    PIRiS46798.
    RefSeqiNP_011861.1. NM_001179082.1.

    Genome annotation databases

    EnsemblFungiiYHL002W; YHL002W; YHL002W.
    GeneIDi856387.
    KEGGisce:YHL002W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U10555 Genomic DNA. Translation: AAB68427.1 .
    BK006934 Genomic DNA. Translation: DAA06685.1 .
    PIRi S46798.
    RefSeqi NP_011861.1. NM_001179082.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2PJW X-ray 3.01 H 288-375 [» ]
    DisProti DP00635.
    ProteinModelPortali P38753.
    SMRi P38753. Positions 14-127, 224-271, 288-375.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 36423. 97 interactions.
    DIPi DIP-1742N.
    IntActi P38753. 44 interactions.
    MINTi MINT-386551.
    STRINGi 4932.YHL002W.

    Proteomic databases

    MaxQBi P38753.
    PaxDbi P38753.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YHL002W ; YHL002W ; YHL002W .
    GeneIDi 856387.
    KEGGi sce:YHL002W.

    Organism-specific databases

    CYGDi YHL002w.
    SGDi S000000994. HSE1.

    Phylogenomic databases

    eggNOGi NOG309509.
    GeneTreei ENSGT00700000104139.
    HOGENOMi HOG000094361.
    KOi K04705.
    OMAi PEPEQAY.
    OrthoDBi EOG75MW70.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-31026-MONOMER.

    Miscellaneous databases

    EvolutionaryTracei P38753.
    NextBioi 981893.

    Gene expression databases

    Genevestigatori P38753.

    Family and domain databases

    Gene3Di 1.25.40.90. 1 hit.
    InterProi IPR008942. ENTH_VHS.
    IPR001452. SH3_domain.
    IPR003903. Ubiquitin-int_motif.
    IPR002014. VHS.
    IPR018205. VHS_subgr.
    [Graphical view ]
    Pfami PF00018. SH3_1. 1 hit.
    PF00790. VHS. 1 hit.
    [Graphical view ]
    PRINTSi PR00452. SH3DOMAIN.
    SMARTi SM00326. SH3. 1 hit.
    SM00726. UIM. 1 hit.
    SM00288. VHS. 1 hit.
    [Graphical view ]
    SUPFAMi SSF48464. SSF48464. 1 hit.
    SSF50044. SSF50044. 1 hit.
    PROSITEi PS50002. SH3. 1 hit.
    PS50330. UIM. 1 hit.
    PS50179. VHS. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    2. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    3. "The Vps27p-Hse1p complex binds ubiquitin and mediates endosomal protein sorting."
      Bilodeau P.S., Urbanowski J.L., Winistorfer S.C., Piper R.C.
      Nat. Cell Biol. 4:534-539(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION IN THE ESCRT-0 COMPLEX, FUNCTION OF THE ESCRT-0 COMPLEX, SUBCELLULAR LOCATION.
    4. "Vps27-Hse1 and ESCRT-I complexes cooperate to increase efficiency of sorting ubiquitinated proteins at the endosome."
      Bilodeau P.S., Winistorfer S.C., Kearney W.R., Robertson A.D., Piper R.C.
      J. Cell Biol. 163:237-243(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION OF THE ESCRT-0 COMPLEX.
    5. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    7. "Protein-protein interactions of ESCRT complexes in the yeast Saccharomyces cerevisiae."
      Bowers K., Lottridge J., Helliwell S.B., Goldthwaite L.M., Luzio J.P., Stevens T.H.
      Traffic 5:194-210(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH RSP5; VPS23 AND VPS27, FUNCTION OF THE ESCRT-0 COMPLEX.
    8. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
      Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
      J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-162, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Strain: ADR376.
    9. "Hse1, a component of the yeast Hrs-STAM ubiquitin-sorting complex, associates with ubiquitin peptidases and a ligase to control sorting efficiency into multivesicular bodies."
      Ren J., Kee Y., Huibregtse J.M., Piper R.C.
      Mol. Biol. Cell 18:324-335(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH RSP5 AND UBP7.
    10. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
      Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
      Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-162, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    11. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
      Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
      Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-162, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    12. Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT SER-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiHSE1_YEAST
    AccessioniPrimary (citable) accession number: P38753
    Secondary accession number(s): D3DKR2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1995
    Last sequence update: February 1, 1995
    Last modified: October 1, 2014
    This is version 127 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 358 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families
    3. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    4. Yeast chromosome VIII
      Yeast (Saccharomyces cerevisiae) chromosome VIII: entries and gene names

    External Data

    Dasty 3