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Protein

Class E vacuolar protein-sorting machinery protein HSE1

Gene

HSE1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Component of the ESCRT-0 complex which is the sorting receptor for ubiquitinated cargo proteins at the multivesicular body (MVB) and recruits ESCRT-I to the MVB outer membrane.4 Publications

GO - Molecular functioni

  • ubiquitin binding Source: SGD

GO - Biological processi

  • late endosome to vacuole transport Source: SGD
  • protein targeting to vacuole Source: SGD
Complete GO annotation...

Keywords - Biological processi

Protein transport, Transport

Enzyme and pathway databases

BioCyciYEAST:G3O-31026-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Class E vacuolar protein-sorting machinery protein HSE1
Gene namesi
Name:HSE1
Ordered Locus Names:YHL002W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311 Componenti: Chromosome VIII

Organism-specific databases

CYGDiYHL002w.
EuPathDBiFungiDB:YHL002W.
SGDiS000000994. HSE1.

Subcellular locationi

GO - Cellular componenti

  • endosome Source: SGD
  • endosome membrane Source: UniProtKB-SubCell
  • ESCRT-0 complex Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Endosome, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed1 Publication
Chaini2 – 452451Class E vacuolar protein-sorting machinery protein HSE1PRO_0000202885Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylserine1 Publication
Modified residuei162 – 1621Phosphoserine3 Publications

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiP38753.
PaxDbiP38753.

Interactioni

Subunit structurei

Component of the ESCRT-0 complex composed of HSE1 and VPS27. Interacts with the ESCRT-I subunit VPS23, the UBP7 deubiquitinase and the E3 ligase RSP5.3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
ACF2Q121682EBI-1382,EBI-32973
AIM21P405634EBI-1382,EBI-25376
DOA1P360373EBI-1382,EBI-6017
STP22P256043EBI-1382,EBI-411625
UBBP0CG532EBI-1382,EBI-5333021From a different organism.
UBP7P404534EBI-1382,EBI-19857
VPS27P4034310EBI-1382,EBI-20380

Protein-protein interaction databases

BioGridi36423. 99 interactions.
DIPiDIP-1742N.
IntActiP38753. 44 interactions.
MINTiMINT-386551.
STRINGi4932.YHL002W.

Structurei

Secondary structure

1
452
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi289 – 2946Combined sources
Helixi296 – 31015Combined sources
Helixi316 – 3194Combined sources
Helixi322 – 33110Combined sources
Helixi332 – 3343Combined sources
Helixi335 – 37137Combined sources
Turni372 – 3743Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2PJWX-ray3.01H288-375[»]
DisProtiDP00635.
ProteinModelPortaliP38753.
SMRiP38753. Positions 14-127, 224-271, 288-375.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP38753.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini15 – 145131VHSPROSITE-ProRule annotationAdd
BLAST
Domaini162 – 18120UIMPROSITE-ProRule annotationAdd
BLAST
Domaini217 – 27660SH3PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the STAM family.Curated
Contains 1 SH3 domain.PROSITE-ProRule annotation
Contains 1 UIM (ubiquitin-interacting motif) domain.PROSITE-ProRule annotation
Contains 1 VHS domain.PROSITE-ProRule annotation

Keywords - Domaini

SH3 domain

Phylogenomic databases

eggNOGiNOG309509.
GeneTreeiENSGT00700000104139.
HOGENOMiHOG000094361.
InParanoidiP38753.
KOiK04705.
OMAiPIVNKSP.
OrthoDBiEOG75MW70.

Family and domain databases

Gene3Di1.25.40.90. 1 hit.
InterProiIPR008942. ENTH_VHS.
IPR001452. SH3_domain.
IPR003903. UIM_dom.
IPR002014. VHS.
IPR018205. VHS_subgr.
[Graphical view]
PfamiPF00018. SH3_1. 1 hit.
PF00790. VHS. 1 hit.
[Graphical view]
PRINTSiPR00452. SH3DOMAIN.
SMARTiSM00326. SH3. 1 hit.
SM00726. UIM. 1 hit.
SM00288. VHS. 1 hit.
[Graphical view]
SUPFAMiSSF48464. SSF48464. 1 hit.
SSF50044. SSF50044. 1 hit.
PROSITEiPS50002. SH3. 1 hit.
PS50330. UIM. 1 hit.
PS50179. VHS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P38753-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSSAIKIRN ALLKATDPKL RSDNWQYILD VCDLVKEDPE DNGQEVMSLI
60 70 80 90 100
EKRLEQQDAN VILRTLSLTV SLAENCGSRL RQEISSKNFT SLLYALIESH
110 120 130 140 150
SVHITLKKAV TDVVKQLSDS FKDDPSLRAM GDLYDKIKRK APYLVQPNVP
160 170 180 190 200
EKHNMSTQAD NSDDEELQKA LKMSLFEYEK QKKLQEQEKE SAEVLPQQQQ
210 220 230 240 250
QHQQQNQAPA HKIPAQTVVR RVRALYDLTT NEPDELSFRK GDVITVLEQV
260 270 280 290 300
YRDWWKGALR GNMGIFPLNY VTPIVEPSKE EIEKEKNKEA IVFSQKTTID
310 320 330 340 350
QLHNSLNAAS KTGNSNEVLQ DPHIGDMYGS VTPLRPQVTR MLGKYAKEKE
360 370 380 390 400
DMLSLRQVLA NAERSYNQLM DRAANAHISP PVPGPALYAG MTHANNTPVM
410 420 430 440 450
PPQRQSYQSN EYSPYPSNLP IQHPTNSANN TPQYGYDLGY SVVSQPPPGY

EQ
Length:452
Mass (Da):51,161
Last modified:February 1, 1995 - v1
Checksum:i026267836BBADF69
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U10555 Genomic DNA. Translation: AAB68427.1.
BK006934 Genomic DNA. Translation: DAA06685.1.
PIRiS46798.
RefSeqiNP_011861.1. NM_001179082.1.

Genome annotation databases

EnsemblFungiiYHL002W; YHL002W; YHL002W.
GeneIDi856387.
KEGGisce:YHL002W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U10555 Genomic DNA. Translation: AAB68427.1.
BK006934 Genomic DNA. Translation: DAA06685.1.
PIRiS46798.
RefSeqiNP_011861.1. NM_001179082.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2PJWX-ray3.01H288-375[»]
DisProtiDP00635.
ProteinModelPortaliP38753.
SMRiP38753. Positions 14-127, 224-271, 288-375.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi36423. 99 interactions.
DIPiDIP-1742N.
IntActiP38753. 44 interactions.
MINTiMINT-386551.
STRINGi4932.YHL002W.

Proteomic databases

MaxQBiP38753.
PaxDbiP38753.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYHL002W; YHL002W; YHL002W.
GeneIDi856387.
KEGGisce:YHL002W.

Organism-specific databases

CYGDiYHL002w.
EuPathDBiFungiDB:YHL002W.
SGDiS000000994. HSE1.

Phylogenomic databases

eggNOGiNOG309509.
GeneTreeiENSGT00700000104139.
HOGENOMiHOG000094361.
InParanoidiP38753.
KOiK04705.
OMAiPIVNKSP.
OrthoDBiEOG75MW70.

Enzyme and pathway databases

BioCyciYEAST:G3O-31026-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP38753.
NextBioi981893.
PROiP38753.

Family and domain databases

Gene3Di1.25.40.90. 1 hit.
InterProiIPR008942. ENTH_VHS.
IPR001452. SH3_domain.
IPR003903. UIM_dom.
IPR002014. VHS.
IPR018205. VHS_subgr.
[Graphical view]
PfamiPF00018. SH3_1. 1 hit.
PF00790. VHS. 1 hit.
[Graphical view]
PRINTSiPR00452. SH3DOMAIN.
SMARTiSM00326. SH3. 1 hit.
SM00726. UIM. 1 hit.
SM00288. VHS. 1 hit.
[Graphical view]
SUPFAMiSSF48464. SSF48464. 1 hit.
SSF50044. SSF50044. 1 hit.
PROSITEiPS50002. SH3. 1 hit.
PS50330. UIM. 1 hit.
PS50179. VHS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. "The Vps27p-Hse1p complex binds ubiquitin and mediates endosomal protein sorting."
    Bilodeau P.S., Urbanowski J.L., Winistorfer S.C., Piper R.C.
    Nat. Cell Biol. 4:534-539(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION IN THE ESCRT-0 COMPLEX, FUNCTION OF THE ESCRT-0 COMPLEX, SUBCELLULAR LOCATION.
  4. "Vps27-Hse1 and ESCRT-I complexes cooperate to increase efficiency of sorting ubiquitinated proteins at the endosome."
    Bilodeau P.S., Winistorfer S.C., Kearney W.R., Robertson A.D., Piper R.C.
    J. Cell Biol. 163:237-243(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION OF THE ESCRT-0 COMPLEX.
  5. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  7. "Protein-protein interactions of ESCRT complexes in the yeast Saccharomyces cerevisiae."
    Bowers K., Lottridge J., Helliwell S.B., Goldthwaite L.M., Luzio J.P., Stevens T.H.
    Traffic 5:194-210(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH RSP5; VPS23 AND VPS27, FUNCTION OF THE ESCRT-0 COMPLEX.
  8. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
    Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
    J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-162, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: ADR376.
  9. "Hse1, a component of the yeast Hrs-STAM ubiquitin-sorting complex, associates with ubiquitin peptidases and a ligase to control sorting efficiency into multivesicular bodies."
    Ren J., Kee Y., Huibregtse J.M., Piper R.C.
    Mol. Biol. Cell 18:324-335(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH RSP5 AND UBP7.
  10. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-162, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  11. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-162, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  12. Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT SER-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiHSE1_YEAST
AccessioniPrimary (citable) accession number: P38753
Secondary accession number(s): D3DKR2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: June 24, 2015
This is version 133 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 358 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome VIII
    Yeast (Saccharomyces cerevisiae) chromosome VIII: entries and gene names

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.