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Protein

Class E vacuolar protein-sorting machinery protein HSE1

Gene

HSE1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Component of the ESCRT-0 complex which is the sorting receptor for ubiquitinated cargo proteins at the multivesicular body (MVB) and recruits ESCRT-I to the MVB outer membrane.4 Publications

Miscellaneous

Present with 358 molecules/cell in log phase SD medium.1 Publication

GO - Molecular functioni

  • protein domain specific binding Source: CAFA
  • protein heterodimerization activity Source: CAFA
  • ubiquitin binding Source: SGD

GO - Biological processi

  • ATP export Source: SGD
  • late endosome to vacuole transport Source: SGD
  • multivesicular body sorting pathway Source: CAFA
  • positive regulation of protein maturation Source: CAFA
  • protein secretion Source: CAFA
  • protein targeting to vacuole Source: SGD

Keywordsi

Biological processProtein transport, Transport

Enzyme and pathway databases

BioCyciYEAST:G3O-31026-MONOMER
ReactomeiR-SCE-5689880 Ub-specific processing proteases
R-SCE-6798695 Neutrophil degranulation

Names & Taxonomyi

Protein namesi
Recommended name:
Class E vacuolar protein-sorting machinery protein HSE1
Gene namesi
Name:HSE1
Ordered Locus Names:YHL002W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VIII

Organism-specific databases

EuPathDBiFungiDB:YHL002W
SGDiS000000994 HSE1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Endosome, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources
ChainiPRO_00002028852 – 452Class E vacuolar protein-sorting machinery protein HSE1Add BLAST451

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserineCombined sources1
Modified residuei162PhosphoserineCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiP38753
PaxDbiP38753
PRIDEiP38753

PTM databases

iPTMnetiP38753

Interactioni

Subunit structurei

Component of the ESCRT-0 complex composed of HSE1 and VPS27. Interacts with the ESCRT-I subunit VPS23, the UBP7 deubiquitinase and the E3 ligase RSP5.3 Publications

Binary interactionsi

Show more details

GO - Molecular functioni

  • protein domain specific binding Source: CAFA
  • protein heterodimerization activity Source: CAFA
  • ubiquitin binding Source: SGD

Protein-protein interaction databases

BioGridi36423, 214 interactors
DIPiDIP-1742N
IntActiP38753, 46 interactors
MINTiP38753
STRINGi4932.YHL002W

Structurei

Secondary structure

1452
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi289 – 294Combined sources6
Helixi296 – 310Combined sources15
Helixi316 – 319Combined sources4
Helixi322 – 331Combined sources10
Helixi332 – 334Combined sources3
Helixi335 – 371Combined sources37
Turni372 – 374Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2PJWX-ray3.01H288-375[»]
ProteinModelPortaliP38753
SMRiP38753
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP38753

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini15 – 145VHSPROSITE-ProRule annotationAdd BLAST131
Domaini162 – 181UIMPROSITE-ProRule annotationAdd BLAST20
Domaini217 – 276SH3PROSITE-ProRule annotationAdd BLAST60

Sequence similaritiesi

Belongs to the STAM family.Curated

Keywords - Domaini

SH3 domain

Phylogenomic databases

GeneTreeiENSGT00870000136415
HOGENOMiHOG000094361
InParanoidiP38753
KOiK04705
OMAiHGGNYMG
OrthoDBiEOG092C454D

Family and domain databases

Gene3Di1.25.40.90, 1 hit
InterProiView protein in InterPro
IPR008942 ENTH_VHS
IPR036028 SH3-like_dom_sf
IPR001452 SH3_domain
IPR003903 UIM_dom
IPR002014 VHS_dom
PfamiView protein in Pfam
PF00018 SH3_1, 1 hit
PF00790 VHS, 1 hit
PRINTSiPR00452 SH3DOMAIN
SMARTiView protein in SMART
SM00326 SH3, 1 hit
SM00726 UIM, 1 hit
SM00288 VHS, 1 hit
SUPFAMiSSF48464 SSF48464, 1 hit
SSF50044 SSF50044, 1 hit
PROSITEiView protein in PROSITE
PS50002 SH3, 1 hit
PS50330 UIM, 1 hit
PS50179 VHS, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P38753-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSSAIKIRN ALLKATDPKL RSDNWQYILD VCDLVKEDPE DNGQEVMSLI
60 70 80 90 100
EKRLEQQDAN VILRTLSLTV SLAENCGSRL RQEISSKNFT SLLYALIESH
110 120 130 140 150
SVHITLKKAV TDVVKQLSDS FKDDPSLRAM GDLYDKIKRK APYLVQPNVP
160 170 180 190 200
EKHNMSTQAD NSDDEELQKA LKMSLFEYEK QKKLQEQEKE SAEVLPQQQQ
210 220 230 240 250
QHQQQNQAPA HKIPAQTVVR RVRALYDLTT NEPDELSFRK GDVITVLEQV
260 270 280 290 300
YRDWWKGALR GNMGIFPLNY VTPIVEPSKE EIEKEKNKEA IVFSQKTTID
310 320 330 340 350
QLHNSLNAAS KTGNSNEVLQ DPHIGDMYGS VTPLRPQVTR MLGKYAKEKE
360 370 380 390 400
DMLSLRQVLA NAERSYNQLM DRAANAHISP PVPGPALYAG MTHANNTPVM
410 420 430 440 450
PPQRQSYQSN EYSPYPSNLP IQHPTNSANN TPQYGYDLGY SVVSQPPPGY

EQ
Length:452
Mass (Da):51,161
Last modified:February 1, 1995 - v1
Checksum:i026267836BBADF69
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U10555 Genomic DNA Translation: AAB68427.1
BK006934 Genomic DNA Translation: DAA06685.1
PIRiS46798
RefSeqiNP_011861.1, NM_001179082.1

Genome annotation databases

EnsemblFungiiYHL002W; YHL002W; YHL002W
GeneIDi856387
KEGGisce:YHL002W

Similar proteinsi

Entry informationi

Entry nameiHSE1_YEAST
AccessioniPrimary (citable) accession number: P38753
Secondary accession number(s): D3DKR2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: April 25, 2018
This is version 158 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome
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Main funding by: National Institutes of Health