ID   SHU1_YEAST              Reviewed;         150 AA.
AC   P38751; D3DKQ9; Q07190;
DT   01-FEB-1995, integrated into UniProtKB/Swiss-Prot.
DT   30-JUL-2004, sequence version 2.
DT   27-MAR-2024, entry version 131.
DE   RecName: Full=Suppressor of HU sensitivity involved in recombination protein 1;
GN   Name=SHU1; OrderedLocusNames=YHL006C;
OS   Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX   NCBI_TaxID=559292;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=8091229; DOI=10.1126/science.8091229;
RA   Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Dover J., Du Z.,
RA   Favello A., Fulton L., Gattung S., Geisel C., Kirsten J., Kucaba T.,
RA   Hillier L.W., Jier M., Johnston L., Langston Y., Latreille P., Louis E.J.,
RA   Macri C., Mardis E., Menezes S., Mouser L., Nhan M., Rifkin L., Riles L.,
RA   St Peter H., Trevaskis E., Vaughan K., Vignati D., Wilcox L., Wohldman P.,
RA   Waterston R., Wilson R., Vaudin M.;
RT   "Complete nucleotide sequence of Saccharomyces cerevisiae chromosome
RT   VIII.";
RL   Science 265:2077-2082(1994).
RN   [2]
RP   SEQUENCE REVISION TO C-TERMINUS.
RA   Fisk D., Cherry J.M.;
RL   Submitted (FEB-2004) to the EMBL/GenBank/DDBJ databases.
RN   [3]
RP   GENOME REANNOTATION.
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=24374639; DOI=10.1534/g3.113.008995;
RA   Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA   Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA   Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT   "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL   G3 (Bethesda) 4:389-398(2014).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RA   Dignard D.;
RL   Submitted (MAY-1994) to the EMBL/GenBank/DDBJ databases.
RN   [5]
RP   IDENTIFICATION IN THE SHU COMPLEX, SUBCELLULAR LOCATION, AND FUNCTION.
RX   PubMed=15654096; DOI=10.1534/genetics.104.036764;
RA   Shor E., Weinstein J., Rothstein R.;
RT   "A genetic screen for top3 suppressors in Saccharomyces cerevisiae
RT   identifies SHU1, SHU2, PSY3 and CSM2: four genes involved in error-free DNA
RT   repair.";
RL   Genetics 169:1275-1289(2005).
RN   [6]
RP   IDENTIFICATION IN THE SHU COMPLEX, AND FUNCTION.
RX   PubMed=19496932; DOI=10.1111/j.1365-2958.2009.06748.x;
RA   Ball L.G., Zhang K., Cobb J.A., Boone C., Xiao W.;
RT   "The yeast Shu complex couples error-free post-replication repair to
RT   homologous recombination.";
RL   Mol. Microbiol. 73:89-102(2009).
CC   -!- FUNCTION: Plays a role in a RAD51/RAD54-dependent homologous
CC       recombination repair (HRR) pathway to repair MMS-induced lesions during
CC       S-phase. {ECO:0000269|PubMed:15654096, ECO:0000269|PubMed:19496932}.
CC   -!- SUBUNIT: Component of the SHU complex composed of at least CSM2, PSY3,
CC       SHU1 and SHU2. {ECO:0000269|PubMed:15654096,
CC       ECO:0000269|PubMed:19496932}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:15654096}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAA35196.1; Type=Frameshift; Evidence={ECO:0000305};
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DR   EMBL; U11581; AAB69748.2; -; Genomic_DNA.
DR   EMBL; L09261; AAA35196.1; ALT_FRAME; Genomic_DNA.
DR   EMBL; BK006934; DAA06682.1; -; Genomic_DNA.
DR   PIR; S46822; S46822.
DR   RefSeq; NP_011857.2; NM_001179086.1.
DR   PDB; 5XYN; X-ray; 3.30 A; C=1-150.
DR   PDBsum; 5XYN; -.
DR   AlphaFoldDB; P38751; -.
DR   SMR; P38751; -.
DR   BioGRID; 36420; 80.
DR   ComplexPortal; CPX-3087; Shu complex.
DR   DIP; DIP-1874N; -.
DR   IntAct; P38751; 7.
DR   MINT; P38751; -.
DR   STRING; 4932.YHL006C; -.
DR   PaxDb; 4932-YHL006C; -.
DR   PeptideAtlas; P38751; -.
DR   EnsemblFungi; YHL006C_mRNA; YHL006C; YHL006C.
DR   GeneID; 856383; -.
DR   KEGG; sce:YHL006C; -.
DR   AGR; SGD:S000000998; -.
DR   SGD; S000000998; SHU1.
DR   VEuPathDB; FungiDB:YHL006C; -.
DR   HOGENOM; CLU_1741621_0_0_1; -.
DR   InParanoid; P38751; -.
DR   OMA; FRIKRKH; -.
DR   OrthoDB; 2026244at2759; -.
DR   BioCyc; YEAST:G3O-31028-MONOMER; -.
DR   BioGRID-ORCS; 856383; 6 hits in 10 CRISPR screens.
DR   ChiTaRS; SHU1; yeast.
DR   PRO; PR:P38751; -.
DR   Proteomes; UP000002311; Chromosome VIII.
DR   RNAct; P38751; Protein.
DR   GO; GO:0005730; C:nucleolus; IDA:SGD.
DR   GO; GO:0097196; C:Shu complex; IDA:SGD.
DR   GO; GO:0035861; C:site of double-strand break; IMP:SGD.
DR   GO; GO:0000730; P:DNA recombinase assembly; IMP:SGD.
DR   GO; GO:0042275; P:error-free postreplication DNA repair; NAS:ComplexPortal.
DR   GO; GO:0043007; P:maintenance of rDNA; IGI:SGD.
DR   GO; GO:1903112; P:positive regulation of single-strand break repair via homologous recombination; NAS:ComplexPortal.
DR   GO; GO:0000725; P:recombinational repair; IMP:SGD.
PE   1: Evidence at protein level;
KW   3D-structure; DNA damage; DNA recombination; DNA repair; Nucleus;
KW   Reference proteome.
FT   CHAIN           1..150
FT                   /note="Suppressor of HU sensitivity involved in
FT                   recombination protein 1"
FT                   /id="PRO_0000097742"
FT   CONFLICT        138
FT                   /note="P -> A (in Ref. 4; AAA35196)"
FT                   /evidence="ECO:0000305"
FT   HELIX           3..11
FT                   /evidence="ECO:0007829|PDB:5XYN"
FT   STRAND          24..30
FT                   /evidence="ECO:0007829|PDB:5XYN"
FT   HELIX           31..38
FT                   /evidence="ECO:0007829|PDB:5XYN"
FT   TURN            39..45
FT                   /evidence="ECO:0007829|PDB:5XYN"
FT   HELIX           51..59
FT                   /evidence="ECO:0007829|PDB:5XYN"
FT   STRAND          61..66
FT                   /evidence="ECO:0007829|PDB:5XYN"
FT   HELIX           69..81
FT                   /evidence="ECO:0007829|PDB:5XYN"
FT   STRAND          83..85
FT                   /evidence="ECO:0007829|PDB:5XYN"
FT   STRAND          89..95
FT                   /evidence="ECO:0007829|PDB:5XYN"
FT   HELIX           96..99
FT                   /evidence="ECO:0007829|PDB:5XYN"
FT   HELIX           105..119
FT                   /evidence="ECO:0007829|PDB:5XYN"
FT   STRAND          122..130
FT                   /evidence="ECO:0007829|PDB:5XYN"
FT   HELIX           137..149
FT                   /evidence="ECO:0007829|PDB:5XYN"
SQ   SEQUENCE   150 AA;  17121 MW;  8B17700BDAA2367F CRC64;
     MQFEERLQQL VESDWSLDQS SPNVLVIVLG DTARKYVELG GLKEHVTTNT VAGHVASRER
     VSVVFLGRVK YLYMYLTRMQ AQANGPQYSN VLVYGLWDLT ATQDGPQQLR LLSLVLRQCL
     SLPSKVEFYP EPPSSSVPAR LLRFWDHIIR
//