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P38749 (YAP3_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 111. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
AP-1-like transcription factor YAP3
Gene names
Name:YAP3
Ordered Locus Names:YHL009C
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length330 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Transcription activator involved in the regulation of genes expressed in response to environmental changes. When overexpressed it activates transcription of the multidrug resistance ABC transporter PDR5, thus conferring resistance to the fungicide fluconazole (FCZ) and cycloheximide. When overexpressed, it also confers, independent of PDR5, increased resistance to 4-nitroquinoline-N-oxide (4-NQO) By similarity. Preferentially binds 5'-TTACTAA-3'. Ref.3 Ref.4 Ref.5 Ref.6

Subunit structure

Homodimer By similarity. Interacts with the C-terminal, cytoplasmic tail of the multidrug resistance ABC transporter PDR5. Ref.6

Subcellular location

Cytoplasm. Nucleus Ref.7.

Miscellaneous

One of 8 closely related fungi-specific YAP proteins (YAP1 to YAP8), which all seem to be transcription activators of the environmental stress response and metabolism control pathways and to have similar but not identical DNA binding specificities.

Present with 1700 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the bZIP family. YAP subfamily.

Contains 1 bZIP (basic-leucine zipper) domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 330330AP-1-like transcription factor YAP3
PRO_0000076523

Regions

Domain144 – 20764bZIP
Region147 – 16822Basic motif By similarity
Region172 – 20736Leucine-zipper By similarity
Compositional bias107 – 1126Poly-Asn

Amino acid modifications

Modified residue1351Phosphoserine Ref.10

Sequences

Sequence LengthMass (Da)Tools
P38749 [UniParc].

Last modified February 1, 1995. Version 1.
Checksum: 9868389698623A27

FASTA33037,955
        10         20         30         40         50         60 
MTPSNMDDNT SGFMKFINPQ CQEEDCCIRN SLFQEDSKCI KQQPDLLSEQ TAPFPILEDQ 

        70         80         90        100        110        120 
CPALNLDRSN NDLLLQNNIS FPKGSDLQAI QLTPISGDYS TYVMADNNNN DNDSYSNTNY 

       130        140        150        160        170        180 
FSKNNGISPS SRSPSVAHNE NVPDDSKAKK KAQNRAAQKA FRERKEARMK ELQDKLLESE 

       190        200        210        220        230        240 
RNRQSLLKEI EELRKANTEI NAENRLLLRS GNENFSKDIE DDTNYKYSFP TKDEFFTSMV 

       250        260        270        280        290        300 
LESKLNHKGK YSLKDNEIMK RNTQYTDEAG RHVLTVPATW EYLYKLSEER DFDVTYVMSK 

       310        320        330 
LQGQECCHTH GPAYPRSLID FLVEEATLNE 

« Hide

References

« Hide 'large scale' references
[1]"Complete nucleotide sequence of Saccharomyces cerevisiae chromosome VIII."
Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Dover J., Du Z., Favello A., Fulton L., Gattung S., Geisel C., Kirsten J., Kucaba T., Hillier L.W., Jier M., Johnston L., Langston Y., Latreille P. expand/collapse author list , Louis E.J., Macri C., Mardis E., Menezes S., Mouser L., Nhan M., Rifkin L., Riles L., St Peter H., Trevaskis E., Vaughan K., Vignati D., Wilcox L., Wohldman P., Waterston R., Wilson R., Vaudin M.
Science 265:2077-2082(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[2]"The reference genome sequence of Saccharomyces cerevisiae: Then and now."
Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.
G3 (Bethesda) 4:389-398(2014) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[3]"Yap, a novel family of eight bZIP proteins in Saccharomyces cerevisiae with distinct biological functions."
Fernandes L., Rodrigues-Pousada C., Struhl K.
Mol. Cell. Biol. 17:6982-6993(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, ISOLATION OF YAP FAMILY PROTEINS.
[4]"Isolation of a putative Candida albicans transcriptional regulator involved in pleiotropic drug resistance by functional complementation of a pdr1 pdr3 mutation in Saccharomyces cerevisiae."
Talibi D., Raymond M.
J. Bacteriol. 181:231-240(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, MULTIDRUG RESISTANCE.
[5]"Functional isolation of the Candida albicans FCR3 gene encoding a bZip transcription factor homologous to Saccharomyces cerevisiae Yap3p."
Yang X., Talibi D., Weber S., Poisson G., Raymond M.
Yeast 18:1217-1225(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, MULTIDRUG RESISTANCE.
[6]"Two-hybrid-based analysis of protein-protein interactions of the yeast multidrug resistance protein, Pdr5p."
Subba Rao G., Bachhawat A.K., Gupta C.M.
Funct. Integr. Genomics 1:357-366(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INTERACTION WITH PDR5.
[7]"Global analysis of protein localization in budding yeast."
Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K.
Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[8]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[9]"A multidimensional chromatography technology for in-depth phosphoproteome analysis."
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[10]"Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-135, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U11581 Genomic DNA. Translation: AAB69745.1.
BK006934 Genomic DNA. Translation: DAA06677.1.
PIRS46819.
RefSeqNP_011854.1. NM_001179089.1.

3D structure databases

ProteinModelPortalP38749.
SMRP38749. Positions 149-195.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid36414. 44 interactions.
DIPDIP-1668N.
IntActP38749. 10 interactions.
MINTMINT-411090.
STRING4932.YHL009C.

Proteomic databases

MaxQBP38749.
PaxDbP38749.
PRIDEP38749.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYHL009C; YHL009C; YHL009C.
GeneID856377.
KEGGsce:YHL009C.

Organism-specific databases

CYGDYHL009c.
SGDS000001001. YAP3.

Phylogenomic databases

eggNOGNOG40061.
OMAQECCHTH.
OrthoDBEOG7ZGXCV.

Enzyme and pathway databases

BioCycYEAST:G3O-31031-MONOMER.

Gene expression databases

GenevestigatorP38749.

Family and domain databases

InterProIPR004827. bZIP.
[Graphical view]
PfamPF00170. bZIP_1. 1 hit.
[Graphical view]
SMARTSM00338. BRLZ. 1 hit.
[Graphical view]
PROSITEPS50217. BZIP. 1 hit.
PS00036. BZIP_BASIC. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio981867.

Entry information

Entry nameYAP3_YEAST
AccessionPrimary (citable) accession number: P38749
Secondary accession number(s): D3DKQ4
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: June 11, 2014
This is version 111 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome VIII

Yeast (Saccharomyces cerevisiae) chromosome VIII: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families