P38749 (YAP3_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
AP-1-like transcription factor YAP3

Gene names

Name:	YAP3
Ordered Locus Names:	YHL009C

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	330 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Transcription activator involved in the regulation of genes expressed in response to environmental changes. When overexpressed it activates transcription of the multidrug resistance ABC transporter PDR5, thus conferring resistance to the fungicide fluconazole (FCZ) and cycloheximide. When overexpressed, it also confers, independent of PDR5, increased resistance to 4-nitroquinoline-N-oxide (4-NQO) By similarity. Preferentially binds 5'-TTACTAA-3'. Ref.3 Ref.4 Ref.5 Ref.6
Subunit structure	Homodimer By similarity. Interacts with the C-terminal, cytoplasmic tail of the multidrug resistance ABC transporter PDR5. Ref.6
Subcellular location	Cytoplasm. Nucleus Ref.7.
Miscellaneous	One of 8 closely related fungi-specific YAP proteins (YAP1 to YAP8), which all seem to be transcription activators of the environmental stress response and metabolism control pathways and to have similar but not identical DNA binding specificities. Present with 1700 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the bZIP family. YAP subfamily. Contains 1 bZIP (basic-leucine zipper) domain.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Cytoplasm Nucleus
Ligand	DNA-binding
Molecular function	Activator
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	regulation of transcription from RNA polymerase II promoter Inferred from direct assay Ref.3. Source: SGD transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell nucleus Inferred from physical interaction Ref.3. Source: SGD
Molecular_function	sequence-specific DNA binding Inferred from direct assay PubMed 19111667. Source: SGD sequence-specific DNA binding transcription factor activity Inferred from direct assay Ref.3. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 330	330	AP-1-like transcription factor YAP3		PRO_0000076523
Regions
	Domain	144 – 207	64	bZIP
	Region	147 – 168	22	Basic motif By similarity
	Region	172 – 207	36	Leucine-zipper By similarity
	Compositional bias	107 – 112	6	Poly-Asn
Amino acid modifications
	Modified residue	2	1	Phosphothreonine Ref.9
	Modified residue	128	1	Phosphoserine Ref.9
	Modified residue	133	1	Phosphoserine Ref.9

Sequences

	Sequence		Length	Mass (Da)
	P38749 [UniParc]. Last modified February 1, 1995. Version 1. Checksum: 9868389698623A27	FASTA	330	37,955
	10 20 30 40 50 60 MTPSNMDDNT SGFMKFINPQ CQEEDCCIRN SLFQEDSKCI KQQPDLLSEQ TAPFPILEDQ 70 80 90 100 110 120 CPALNLDRSN NDLLLQNNIS FPKGSDLQAI QLTPISGDYS TYVMADNNNN DNDSYSNTNY 130 140 150 160 170 180 FSKNNGISPS SRSPSVAHNE NVPDDSKAKK KAQNRAAQKA FRERKEARMK ELQDKLLESE 190 200 210 220 230 240 RNRQSLLKEI EELRKANTEI NAENRLLLRS GNENFSKDIE DDTNYKYSFP TKDEFFTSMV 250 260 270 280 290 300 LESKLNHKGK YSLKDNEIMK RNTQYTDEAG RHVLTVPATW EYLYKLSEER DFDVTYVMSK 310 320 330 LQGQECCHTH GPAYPRSLID FLVEEATLNE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete nucleotide sequence of Saccharomyces cerevisiae chromosome VIII." Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Dover J., Du Z., Favello A., Fulton L., Gattung S., Geisel C., Kirsten J., Kucaba T., Hillier L.W., Jier M., Johnston L., Langston Y., Latreille P. , Louis E.J., Macri C., Mardis E., Menezes S., Mouser L., Nhan M., Rifkin L., Riles L., St Peter H., Trevaskis E., Vaughan K., Vignati D., Wilcox L., Wohldman P., Waterston R., Wilson R., Vaudin M. Science 265:2077-2082(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[3]	"Yap, a novel family of eight bZIP proteins in Saccharomyces cerevisiae with distinct biological functions." Fernandes L., Rodrigues-Pousada C., Struhl K. Mol. Cell. Biol. 17:6982-6993(1997) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, ISOLATION OF YAP FAMILY PROTEINS.
[4]	"Isolation of a putative Candida albicans transcriptional regulator involved in pleiotropic drug resistance by functional complementation of a pdr1 pdr3 mutation in Saccharomyces cerevisiae." Talibi D., Raymond M. J. Bacteriol. 181:231-240(1999) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, MULTIDRUG RESISTANCE.
[5]	"Functional isolation of the Candida albicans FCR3 gene encoding a bZip transcription factor homologous to Saccharomyces cerevisiae Yap3p." Yang X., Talibi D., Weber S., Poisson G., Raymond M. Yeast 18:1217-1225(2001) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, MULTIDRUG RESISTANCE.
[6]	"Two-hybrid-based analysis of protein-protein interactions of the yeast multidrug resistance protein, Pdr5p." Subba Rao G., Bachhawat A.K., Gupta C.M. Funct. Integr. Genomics 1:357-366(2002) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INTERACTION WITH PDR5.
[7]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[8]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[9]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-2; SER-128 AND SER-133, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U11581 Genomic DNA. Translation: AAB69745.1. BK006934 Genomic DNA. Translation: DAA06677.1.
PIR	S46819.
RefSeq	NP_011854.1. NM_001179089.1.
3D structure databases
ProteinModelPortal	P38749.
SMR	P38749. Positions 149-195.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-1668N.
IntAct	P38749. 9 interactions.
MINT	MINT-411090.
STRING	4932.YHL009C.
Proteomic databases
PaxDb	P38749.
PRIDE	P38749.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YHL009C; YHL009C; YHL009C.
GeneID	856377.
KEGG	sce:YHL009C.
Organism-specific databases
CYGD	YHL009c.
SGD	S000001001. YAP3.
Phylogenomic databases
eggNOG	NOG40061.
OMA	KANTEIN.
OrthoDB	EOG4XH38Z.
Gene expression databases
Genevestigator	P38749.
GermOnline	YHL009C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR004827. bZIP. [Graphical view]
Pfam	PF00170. bZIP_1. 1 hit. [Graphical view]
SMART	SM00338. BRLZ. 1 hit. [Graphical view]
PROSITE	PS50217. BZIP. 1 hit. PS00036. BZIP_BASIC. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	981867.

Entry information

Entry name

YAP3_YEAST

Accession

Primary (citable) accession number: P38749
Secondary accession number(s): D3DKQ4

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	February 1, 1995
Last modified:	May 1, 2013
This is version 101 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program