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Protein

RING finger protein ETP1

Gene

ETP1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

May act as a cytoplasmic retention protein with a role in regulating nuclear transport. Binds nuclear localization sequences in vitro. Needed to adapt efficiently to ethanol, either as sole carbon source or as cell stressor. Involved in ethanol-dependent transcriptional activation of several genes and ethanol-induced protein turnover of some proteins.1 Publication

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri240 – 28041RING-typePROSITE-ProRule annotationAdd
BLAST
Zinc fingeri299 – 36062UBP-typePROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  • nuclear localization sequence binding Source: SGD
  • ubiquitin binding Source: SGD
  • ubiquitin-protein transferase activity Source: GO_Central
  • zinc ion binding Source: InterPro

GO - Biological processi

  • protein ubiquitination Source: GO_Central
  • response to ethanol Source: SGD
Complete GO annotation...

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

BioCyciYEAST:G3O-31032-MONOMER.
ReactomeiR-SCE-5675221. Negative regulation of MAPK pathway.

Names & Taxonomyi

Protein namesi
Recommended name:
RING finger protein ETP1
Alternative name(s):
BRAP2 homolog
Ethanol tolerance protein 1
Gene namesi
Name:ETP1
Ordered Locus Names:YHL010C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VIII

Organism-specific databases

EuPathDBiFungiDB:YHL010C.
SGDiS000001002. ETP1.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 585585RING finger protein ETP1PRO_0000056337Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Cross-linki369 – 369Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)Combined sources1 Publication
Modified residuei376 – 3761PhosphoserineCombined sources
Cross-linki410 – 410Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)2 Publications
Cross-linki450 – 450Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)Combined sources

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

MaxQBiP38748.

PTM databases

iPTMnetiP38748.

Interactioni

GO - Molecular functioni

  • ubiquitin binding Source: SGD

Protein-protein interaction databases

BioGridi36413. 53 interactions.
DIPiDIP-2987N.
IntActiP38748. 1 interaction.
MINTiMINT-436947.

Structurei

3D structure databases

ProteinModelPortaliP38748.
SMRiP38748. Positions 238-282, 301-359.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Contains 1 RING-type zinc finger.PROSITE-ProRule annotation
Contains 1 UBP-type zinc finger.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri240 – 28041RING-typePROSITE-ProRule annotationAdd
BLAST
Zinc fingeri299 – 36062UBP-typePROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Zinc-finger

Phylogenomic databases

GeneTreeiENSGT00500000044909.
HOGENOMiHOG000190616.
InParanoidiP38748.
KOiK10632.
OMAiDISHCRM.
OrthoDBiEOG7RJQ12.

Family and domain databases

Gene3Di3.30.40.10. 2 hits.
InterProiIPR011422. BRAP2.
IPR001841. Znf_RING.
IPR013083. Znf_RING/FYVE/PHD.
IPR001607. Znf_UBP.
[Graphical view]
PfamiPF07576. BRAP2. 1 hit.
PF13639. zf-RING_2. 1 hit.
PF02148. zf-UBP. 1 hit.
[Graphical view]
ProDomiPD017029. BRAP2. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SMARTiSM00184. RING. 1 hit.
SM00290. ZnF_UBP. 1 hit.
[Graphical view]
PROSITEiPS50089. ZF_RING_2. 1 hit.
PS50271. ZF_UBP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P38748-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDQFEYIITL EFGNQNQVES AYQIFKSIPK KLKAKSIGEE SIKSNNQDWQ
60 70 80 90 100
DWRVCDLEID MITDFKNQTS KEEESDLITS QYLGHGIIRL FKLSNANNTL
110 120 130 140 150
NEKEILTIPG DDTMICILFV PTYFTVHDLL HFYIGDDIVN KQVSNFRILR
160 170 180 190 200
NQQKGMGFNF TVLIKFRNAL DAKNFKEEFN GKSFSRMDPE TCHVISVKEI
210 220 230 240 250
VFQKKLFQRP AANEDFPYLL TDPFTVKKKK ELVKVELPTC PVCLERMDSE
260 270 280 290 300
TTGLVTIPCQ HTFHCQCLNK WKNSRCPVCR HSSLRLSRES LLKQAGDSAH
310 320 330 340 350
CATCGSTDNL WICLICGNVG CGRYNSKHAI KHYEETLHCF AMDIRTQRVW
360 370 380 390 400
DYAGDNYVHR LVQNEVDGKL VEVGGSGDDD NNDIGNSDEL QNVVYGNRSK
410 420 430 440 450
NGEKSNSNKK DGELAANFLR HREYHLEYVQ VLISQLESQR EYYELKLQEK
460 470 480 490 500
DQTASDSSNV ESLKKSMEDL KLQFQVTQKE WQKREMAQKS KLEEDMLVIE
510 520 530 540 550
GLQANLDHLS KKQEQLEREN KALEESKQDL EEQVKDLMFY LDSQEKFKDA
560 570 580
DESVKEGTIL IQQPHGAAQA SKSKKKRNKN KKAGK
Length:585
Mass (Da):67,503
Last modified:February 1, 1995 - v1
Checksum:i88FF670CC73A1263
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti558 – 5581T → I in AAS56441 (PubMed:17322287).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U11582 Genomic DNA. Translation: AAB65064.1.
AY558115 Genomic DNA. Translation: AAS56441.1.
BK006934 Genomic DNA. Translation: DAA06676.1.
PIRiS46825.
RefSeqiNP_011853.1. NM_001179090.1.

Genome annotation databases

EnsemblFungiiYHL010C; YHL010C; YHL010C.
GeneIDi856376.
KEGGisce:YHL010C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U11582 Genomic DNA. Translation: AAB65064.1.
AY558115 Genomic DNA. Translation: AAS56441.1.
BK006934 Genomic DNA. Translation: DAA06676.1.
PIRiS46825.
RefSeqiNP_011853.1. NM_001179090.1.

3D structure databases

ProteinModelPortaliP38748.
SMRiP38748. Positions 238-282, 301-359.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi36413. 53 interactions.
DIPiDIP-2987N.
IntActiP38748. 1 interaction.
MINTiMINT-436947.

PTM databases

iPTMnetiP38748.

Proteomic databases

MaxQBiP38748.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYHL010C; YHL010C; YHL010C.
GeneIDi856376.
KEGGisce:YHL010C.

Organism-specific databases

EuPathDBiFungiDB:YHL010C.
SGDiS000001002. ETP1.

Phylogenomic databases

GeneTreeiENSGT00500000044909.
HOGENOMiHOG000190616.
InParanoidiP38748.
KOiK10632.
OMAiDISHCRM.
OrthoDBiEOG7RJQ12.

Enzyme and pathway databases

BioCyciYEAST:G3O-31032-MONOMER.
ReactomeiR-SCE-5675221. Negative regulation of MAPK pathway.

Miscellaneous databases

PROiP38748.

Family and domain databases

Gene3Di3.30.40.10. 2 hits.
InterProiIPR011422. BRAP2.
IPR001841. Znf_RING.
IPR013083. Znf_RING/FYVE/PHD.
IPR001607. Znf_UBP.
[Graphical view]
PfamiPF07576. BRAP2. 1 hit.
PF13639. zf-RING_2. 1 hit.
PF02148. zf-UBP. 1 hit.
[Graphical view]
ProDomiPD017029. BRAP2. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SMARTiSM00184. RING. 1 hit.
SM00290. ZnF_UBP. 1 hit.
[Graphical view]
PROSITEiPS50089. ZF_RING_2. 1 hit.
PS50271. ZF_UBP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. Cited for: UBIQUITINATION [LARGE SCALE ANALYSIS] AT LYS-369 AND LYS-410.
    Strain: SUB592.
  5. "A subset of membrane-associated proteins is ubiquitinated in response to mutations in the endoplasmic reticulum degradation machinery."
    Hitchcock A.L., Auld K., Gygi S.P., Silver P.A.
    Proc. Natl. Acad. Sci. U.S.A. 100:12735-12740(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: UBIQUITINATION [LARGE SCALE ANALYSIS] AT LYS-410.
  6. "ETP1/YHL010c is a novel gene needed for the adaptation of Saccharomyces cerevisiae to ethanol."
    Snowdon C., Schierholtz R., Poliszczuk P., Hughes S., van der Merwe G.
    FEMS Yeast Res. 9:372-380(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  7. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-376, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  8. "Sites of ubiquitin attachment in Saccharomyces cerevisiae."
    Starita L.M., Lo R.S., Eng J.K., von Haller P.D., Fields S.
    Proteomics 12:236-240(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: UBIQUITINATION [LARGE SCALE ANALYSIS] AT LYS-369 AND LYS-450, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiETP1_YEAST
AccessioniPrimary (citable) accession number: P38748
Secondary accession number(s): D3DKQ3, Q6Q5F9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: July 6, 2016
This is version 130 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome VIII
    Yeast (Saccharomyces cerevisiae) chromosome VIII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.