Reviewed, UniProtKB/Swiss-Prot P38748 (ETP1_YEAST)

Names and origin

Protein names

Recommended name:
    RING finger protein ETP1
Alternative name(s):
    Ethanol tolerance protein 1
    BRAP2 homolog

Gene names

Name:	ETP1
Ordered Locus Names:	YHL010C

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	585 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	May act as a cytoplasmic retention protein with a role in regulating nuclear transport. Binds nuclear localization sequences in vitro. Needed to adapt efficiently to ethanol, either as sole carbon source or as cell stressor. Involved in ethanol-dependent transcriptional activation of several genes and ethanol-induced protein turnover of some proteins. Ref.5
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Contains 1 RING-type zinc finger. Contains 1 UBP-type zinc finger.

Ontologies

Keywords
Cellular component	Cytoplasm
Domain	Zinc-finger
Ligand	Metal-binding Zinc
PTM	Isopeptide bond Ubl conjugation
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	response to ethanol Ref.5 Inferred from mutant phenotype. Source: SGD
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	nuclear localization sequence binding Inferred from direct assay. Source: SGD protein binding Inferred from electronic annotation. Source: InterPro zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 585	585	RING finger protein ETP1		PRO_0000056337
Regions
	Zinc finger	240 – 280	41	RING-type
	Zinc finger	299 – 360	62	UBP-type
Amino acid modifications
	Cross-link	369		Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) Ref.3
	Cross-link	410		Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) Ref.3 Ref.4
Experimental info
	Sequence conflict	558	1	T → I in AAS56441. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P38748-1 [UniParc]. Last modified February 1, 1995. Version 1. Checksum: 88FF670CC73A1263	FASTA	585	67,503
	10 20 30 40 50 60 MDQFEYIITL EFGNQNQVES AYQIFKSIPK KLKAKSIGEE SIKSNNQDWQ DWRVCDLEID 70 80 90 100 110 120 MITDFKNQTS KEEESDLITS QYLGHGIIRL FKLSNANNTL NEKEILTIPG DDTMICILFV 130 140 150 160 170 180 PTYFTVHDLL HFYIGDDIVN KQVSNFRILR NQQKGMGFNF TVLIKFRNAL DAKNFKEEFN 190 200 210 220 230 240 GKSFSRMDPE TCHVISVKEI VFQKKLFQRP AANEDFPYLL TDPFTVKKKK ELVKVELPTC 250 260 270 280 290 300 PVCLERMDSE TTGLVTIPCQ HTFHCQCLNK WKNSRCPVCR HSSLRLSRES LLKQAGDSAH 310 320 330 340 350 360 CATCGSTDNL WICLICGNVG CGRYNSKHAI KHYEETLHCF AMDIRTQRVW DYAGDNYVHR 370 380 390 400 410 420 LVQNEVDGKL VEVGGSGDDD NNDIGNSDEL QNVVYGNRSK NGEKSNSNKK DGELAANFLR 430 440 450 460 470 480 HREYHLEYVQ VLISQLESQR EYYELKLQEK DQTASDSSNV ESLKKSMEDL KLQFQVTQKE 490 500 510 520 530 540 WQKREMAQKS KLEEDMLVIE GLQANLDHLS KKQEQLEREN KALEESKQDL EEQVKDLMFY 550 560 570 580 LDSQEKFKDA DESVKEGTIL IQQPHGAAQA SKSKKKRNKN KKAGK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete nucleotide sequence of Saccharomyces cerevisiae chromosome VIII." Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Dover J., Du Z., Favello A., Fulton L., Gattung S., Geisel C., Kirsten J., Kucaba T., Hillier L.W., Jier M., Johnston L., Langston Y., Latreille P. , Louis E.J., Macri C., Mardis E., Menezes S., Mouser L., Nhan M., Rifkin L., Riles L., St Peter H., Trevaskis E., Vaughan K., Vignati D., Wilcox L., Wohldman P., Waterston R., Wilson R., Vaudin M. Science 265:2077-2082(1994) [PubMed: 8091229] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed: 17322287] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[3]	"A proteomics approach to understanding protein ubiquitination." Peng J., Schwartz D., Elias J.E., Thoreen C.C., Cheng D., Marsischky G., Roelofs J., Finley D., Gygi S.P. Nat. Biotechnol. 21:921-926(2003) [PubMed: 12872131] [Abstract] Cited for: UBIQUITINATION [LARGE SCALE ANALYSIS] AT LYS-369 AND LYS-410, MASS SPECTROMETRY.
[4]	"A subset of membrane-associated proteins is ubiquitinated in response to mutations in the endoplasmic reticulum degradation machinery." Hitchcock A.L., Auld K., Gygi S.P., Silver P.A. Proc. Natl. Acad. Sci. U.S.A. 100:12735-12740(2003) [PubMed: 14557538] [Abstract] Cited for: UBIQUITINATION [LARGE SCALE ANALYSIS] AT LYS-410, MASS SPECTROMETRY.
[5]	"ETP1/YHL010c is a novel gene needed for the adaptation of Saccharomyces cerevisiae to ethanol." Snowdon C., Schierholtz R., Poliszczuk P., Hughes S., van der Merwe G. FEMS Yeast Res. 9:372-380(2009) [PubMed: 19416103] [Abstract] Cited for: FUNCTION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U11582 Genomic DNA. Translation: AAB65064.1. AY558115 Genomic DNA. Translation: AAS56441.1.
PIR	S46825.
RefSeq	NP_011853.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:2987N.
IntAct	P38748. 4 interactions.
STRING	P38748.
Genome annotation databases
Ensembl	YHL010C; YHL010C; YHL010C; Saccharomyces cerevisiae. [Genome view]
GeneID	856376.
KEGG	sce:YHL010C.
NMPDR	fig|4932.3.peg.2993.
Organism-specific databases
CYGD	YHL010c.
SGD	S000001002. YHL010C.
Phylogenomic databases
HOGENOM	P38748.
OMA	ETIVDEY
OrthoDB	EOG92V9Z9
Gene expression databases
ArrayExpress	P38748.
Genevestigator	P38748.
GermOnline	YHL010C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR011422. BRAP2. IPR018957. Znf_C3HC4_RING-type. IPR001841. Znf_RING. IPR001607. Znf_UBP. [Graphical view]
Pfam	PF07576. BRAP2. 1 hit. PF00097. zf-C3HC4. 1 hit. PF02148. zf-UBP. 1 hit. [Graphical view]
ProDom	PD017029. BRAP2. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00184. RING. 1 hit. SM00290. ZnF_UBP. 1 hit. [Graphical view]
PROSITE	PS00518. ZF_RING_1. False negative. PS50089. ZF_RING_2. 1 hit. PS50271. ZF_UBP. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	981864.

Entry information

Entry name

ETP1_YEAST

Accession

Primary (citable) accession number: P38748
Secondary accession number(s): Q6Q5F9

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	February 1, 1995
Last modified:	November 24, 2009
This is version 71 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)