Reviewed, UniProtKB/Swiss-Prot P38746 (YLF2_YEAST)

Names and origin

Protein names

Recommended name:
    Putative GTP-binding protein YLF2

Gene names

Name:	YLF2
Ordered Locus Names:	YHL014C

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	405 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Miscellaneous	Present with 2360 molecules/cell in log phase SD medium. Ref.3
Sequence similarities	Belongs to the GTP1/OBG family.

Ontologies

Keywords
Ligand	GTP-binding Nucleotide-binding
Technical term	Complete proteome
Gene Ontology (GO)
Cellular component	mitochondrion Inferred from direct assay. Source: SGD
Molecular function	GTP binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 405	405	Putative GTP-binding protein YLF2		PRO_0000122455
Regions
	Nucleotide binding	23 – 30	8	GTP By similarity
	Nucleotide binding	87 – 91	5	GTP By similarity
Experimental info
	Sequence conflict	63	1	S → T in CAA82332. Ref.1
	Sequence conflict	149	1	L → F in CAA82332. Ref.1
	Sequence conflict	323	1	E → A in CAA82332. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P38746-1 [UniParc]. Last modified February 1, 1995. Version 1. Checksum: DF25FBCB6BE80AEC	FASTA	405	45,715
	10 20 30 40 50 60 MNIGGGKFLL GRISNNPTSG IVGLANVGKS TFFQAITNSK LGNPANYPFA TIDAECAKVN 70 80 90 100 110 120 IPSVPLSNLL RIYQSAKCVP GTLTIYDIAG LTRGASQGHG LGNKFLNDIR HVEGIFQVVR 130 140 150 160 170 180 GFLKEDITHI EGNVDPVRDL SVVQDELILK DLEFLENIRE RLSKKMRMVS KNSKEHQEMK 190 200 210 220 230 240 IETELLDALE EHLFNGKKIR HFKDHWNLDE VKILNKHNFL TSKPTLILLN VSPQDYVRNE 250 260 270 280 290 300 NKFVRNIIEW INEFSPGDKF LLFSAEFESQ LMECKGIASE YFDKIKEDTN VSDQQLVSAI 310 320 330 340 350 360 PQIILEMRKL LNLISFFTCG PQEVHQWNIR EGTTAQEAAG VIHSDLRETF ISADVIKYDD 370 380 390 400 LKKMEPPLNE SLLKSKGLIK RAGKQYIMQD NDIALFKAAG GKIKK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Suppression of yeast RNA polymerase III mutations by the URP2 gene encoding a protein homologous to the mammalian ribosomal protein S20." Hermann-Le Denmat S., Sipickzki M., Thuriaux P. J. Mol. Biol. 240:1-7(1994) [PubMed: 8021936] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 28383 / FL100 / VTT C-80102.
[2]	"Complete nucleotide sequence of Saccharomyces cerevisiae chromosome VIII." Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Dover J., Du Z., Favello A., Fulton L., Gattung S., Geisel C., Kirsten J., Kucaba T., Hillier L.W., Jier M., Johnston L., Langston Y., Latreille P. , Louis E.J., Macri C., Mardis E., Menezes S., Mouser L., Nhan M., Rifkin L., Riles L., St Peter H., Trevaskis E., Vaughan K., Vignati D., Wilcox L., Wohldman P., Waterston R., Wilson R., Vaudin M. Science 265:2077-2082(1994) [PubMed: 8091229] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[3]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	Z29089 Genomic DNA. Translation: CAA82332.1. U11582 Genomic DNA. Translation: AAB65067.1.
PIR	S46828.
RefSeq	NP_011849.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
IntAct	P38746. 1 interaction.
STRING	P38746.
Genome annotation databases
Ensembl	YHL014C; YHL014C; YHL014C; Saccharomyces cerevisiae. [Genome view]
GeneID	856372.
KEGG	sce:YHL014C.
NMPDR	fig|4932.3.peg.2989.
Organism-specific databases
CYGD	YHL014c.
SGD	S000001006. YLF2.
Phylogenomic databases
HOGENOM	P38746.
OMA	ISFYTCG
OrthoDB	EOG9PCC83
Gene expression databases
ArrayExpress	P38746.
Genevestigator	P38746.
GermOnline	YHL014C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR012675. b-grasp_ferredoxin-like. IPR004396. CHP00092. IPR013029. DUF933. IPR006073. GTP1_OBG. IPR002917. MMR_HSR1_GTP_bd. IPR012676. TGS-like. [Graphical view]
Gene3D	G3DSA:3.10.20.30. Ferredoxin_fold. 1 hit.
Pfam	PF01926. MMR_HSR1. 1 hit. PF06071. YchF-GTPase_C. 1 hit. [Graphical view]
PIRSF	PIRSF006641. CHP00092. 1 hit.
PRINTS	PR00326. GTP1OBG.
TIGRFAMs	TIGR00092. CHP92. 1 hit.
ProtoNet	Search...
Other Resources
NextBio	981852.

Entry information

Entry name

YLF2_YEAST

Accession

Primary (citable) accession number: P38746

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	February 1, 1995
Last modified:	November 24, 2009
This is version 70 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)