P38742 (NPR3_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Nitrogen permease regulator 3

Alternative name(s):
Required for meiotic nuclear division protein 11

Gene names

Name:	NPR3
Synonyms:	RMD11
Ordered Locus Names:	YHL023C

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	1146 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Mediates inactivation of the TORC1 complex in response to amino acid starvation. Required for meiotic nuclear division. Ref.3 Ref.9
Subunit structure	Forms a heterodimer with NPR2.
Sequence similarities	Belongs to the NPR3 family.

Ontologies

Keywords
Biological process	Meiosis
Domain	Signal
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	cellular response to amino acid starvation Inferred from mutant phenotype Ref.9. Source: SGD meiosis Inferred from mutant phenotype. Source: SGD regulation of TOR signaling cascade Inferred from mutant phenotype Ref.9. Source: SGD
Cellular component	Seh1-associated complex Inferred from direct assay. Source: SGD extrinsic to fungal-type vacuolar membrane Inferred from direct assay. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
NPR2	P39923	1	EBI-24336,EBI-12212

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 25	25	Potential
	Chain	26 – 1146	1121	Nitrogen permease regulator 3		PRO_0000202882
Regions
	Compositional bias	149 – 315	167	Ser-rich
Amino acid modifications
	Modified residue	76	1	Phosphoserine Ref.4 Ref.8
	Modified residue	117	1	Phosphoserine Ref.7
	Modified residue	120	1	Phosphoserine Ref.7
	Modified residue	456	1	Phosphoserine Ref.8
	Modified residue	477	1	Phosphoserine Ref.8
	Modified residue	480	1	Phosphothreonine Ref.8
	Modified residue	486	1	Phosphoserine Ref.5 Ref.7 Ref.8
	Modified residue	885	1	Phosphoserine Ref.8
	Modified residue	892	1	Phosphotyrosine Ref.8
	Modified residue	987	1	Phosphoserine Ref.6

Sequences

	Sequence		Length	Mass (Da)
	P38742 [UniParc]. Last modified February 1, 1995. Version 1. Checksum: 8580F0C58982D51E	FASTA	1,146	129,973
	10 20 30 40 50 60 MDECLPNSCL LGVHLVISTH SGPQIVYHYP PSNTAFLTNN PTKHQHLYGN HANLNKNTST 70 80 90 100 110 120 NKEEKLFNSG STKTASQIAL NESAKSYNTA ITPSMTNTNT NNVTLPPTRS HANTVGSQSS 130 140 150 160 170 180 IPAATNGVGY RKTDIEDTSR TFQYQETESE TSSSGLSDSE LSTDYLDISS DSFSISSSLS 190 200 210 220 230 240 SSSLSSSPSS SSSSSPPQDG LSRTNSSFQS TDSMSPTSPQ MIMENDSISV AESYLDSGTN 250 260 270 280 290 300 NKSRAASKRS QNFFHKLSTK KSTDSKTHSP VRKLKSKPSQ STKKGNKLLK NTSNETDGNA 310 320 330 340 350 360 FTGSCSISSK KSLSSTGEHN QELRNSSLND TPGQSPHHYH HRYHHYHKNA ATSQRNSHTQ 370 380 390 400 410 420 YDVEEEDMEV SAMLQDGKIS MNEIFFEEEN FQDINKILEF DNDFVAEFCS PEREMCNTRF 430 440 450 460 470 480 EFTVDNFCFL GLPIHVDSQG RWRKSKHKNK TRSKRSSSTT TNISRKKSIA SKISSLSENT 490 500 510 520 530 540 LKKVNSGEAD TVYDSNIGHE ASTDTPNLRI NTDVSGNEFE REKEDLGKNM NMFHVCFVMN 550 560 570 580 590 600 PHLIEYNKRI DDMYQFVVTR LSLLLRYVQS KTSYISSECH IILKEKERVL KHSKTYQSIR 610 620 630 640 650 660 GAGNKGKYLY QRILAKSSLA RALTECVDKI QRNEIACLEI NDDKVISLQI PIQNEFEKMP 670 680 690 700 710 720 NFKLQPVLRG SYLTSILNMK FLEKSSLRIE SQNRQNDQAQ FSDTNNNIYR FGNNINSTGH 730 740 750 760 770 780 CGAANVDDGD DNESNYYCDD NDDLLNYALL LLDEPNNIIS SLETFSYQDD IGTIILKHLV 790 800 810 820 830 840 RNIQPNIPLR SYRYLITDLL DNPSSLDDLT TETNSLESSI LRSCALHLMY WRHARIVIPL 850 860 870 880 890 900 SSKYTYIVSP LAPIQGYTID DYKSTSQNDG NVKKMDDREN NKSGSDRVPL IYQNSMLFRS 910 920 930 940 950 960 KFPSLPSLPI FLSLLSTDKP QAYSNIIPSR EHKPVYLNAL AWLIQYGYVT QLLTFINIRV 970 980 990 1000 1010 1020 DKHIKMAVDE DLEKEGFRKT NTARRPSMDY KKTDKKLDDE DGQSRDANAS EACSGKNEGM 1030 1040 1050 1060 1070 1080 QSNDNNKDVD EKDNENDSRV DDRDDNEIAI ADEEEILHFE YDDPEMQHDY TIILEPERAT 1090 1100 1110 1120 1130 1140 AIEKRWLYRC IYGQPSDIQI LFNKLLKYFN GKVPMELVII KEEISRHDLK KLLNALDKYL IEIHHW

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete nucleotide sequence of Saccharomyces cerevisiae chromosome VIII." Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Dover J., Du Z., Favello A., Fulton L., Gattung S., Geisel C., Kirsten J., Kucaba T., Hillier L.W., Jier M., Johnston L., Langston Y., Latreille P. , Louis E.J., Macri C., Mardis E., Menezes S., Mouser L., Nhan M., Rifkin L., Riles L., St Peter H., Trevaskis E., Vaughan K., Vignati D., Wilcox L., Wohldman P., Waterston R., Wilson R., Vaudin M. Science 265:2077-2082(1994) [PubMed: 8091229] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[3]	"Large-scale functional genomic analysis of sporulation and meiosis in Saccharomyces cerevisiae." Enyenihi A.H., Saunders W.S. Genetics 163:47-54(2003) [PubMed: 12586695] [Abstract] Cited for: FUNCTION.
[4]	"Quantitative phosphoproteomics applied to the yeast pheromone signaling pathway." Gruhler A., Olsen J.V., Mohammed S., Mortensen P., Faergeman N.J., Mann M., Jensen O.N. Mol. Cell. Proteomics 4:310-327(2005) [PubMed: 15665377] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-76, MASS SPECTROMETRY. Strain: YAL6B.
[5]	"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae." Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P. J. Proteome Res. 6:1190-1197(2007) [PubMed: 17330950] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-486, MASS SPECTROMETRY. Strain: ADR376.
[6]	"Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry." Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F. Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed: 17287358] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-987, MASS SPECTROMETRY.
[7]	"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H. Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed: 17563356] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-117; SER-120 AND SER-486.
[8]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-76; SER-456; SER-477; THR-480; SER-486; SER-885 AND TYR-892, MASS SPECTROMETRY.
[9]	"A genome-wide screen for regulators of TORC1 in response to amino acid starvation reveals a conserved Npr2/3 complex." Neklesa T.K., Davis R.W. PLoS Genet. 5:E1000515-E1000515(2009) [PubMed: 19521502] [Abstract] Cited for: FUNCTION, INTERACTION WITH NPR2.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U11582 Genomic DNA. Translation: AAB65076.1. BK006934 Genomic DNA. Translation: DAA06662.1.
PIR	S46837.
RefSeq	NP_011840.1. NM_001179103.1.
3D structure databases
ProteinModelPortal	P38742.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-2087N.
IntAct	P38742. 4 interactions.
MINT	MINT-538335.
STRING	P38742.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YHL023C; YHL023C; YHL023C.
GeneID	856362.
KEGG	sce:YHL023C.
NMPDR	fig|4932.3.peg.2978.
Organism-specific databases
CYGD	YHL023c.
SGD	S000001015. NPR3.
Phylogenomic databases
eggNOG	fuNOG08592.
GeneTree	EFGT00050000002956.
HOGENOM	HBG203824.
OMA	ARALTEC.
OrthoDB	EOG42858X.
Gene expression databases
ArrayExpress	P38742.
Genevestigator	P38742.
GermOnline	YHL023C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR005365. NPR3. [Graphical view]
PANTHER	PTHR13153. UPF0171. 1 hit.
Pfam	PF03666. NPR3. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	981823.

Entry information

Entry name

NPR3_YEAST

Accession

Primary (citable) accession number: P38742
Secondary accession number(s): D3DKU5

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	February 1, 1995
Last modified:	December 14, 2011
This is version 80 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program