P38741 (RIM4_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Meiotic activator RIM4

Alternative name(s):
Regulator of IME2 protein 4

Gene names

Name:	RIM4
Ordered Locus Names:	YHL024W

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	713 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Positive regulator of sporulation-specific genes and of sporulation. Required for premeiotic DNA synthesis and meiotic chromosomal segregation. May act in a nutritional signaling pathway. Ref.4 Ref.5 Ref.6
Induction	Expressed at elevated levels early in meiosis. Expression depends on IME1. Ref.5 Ref.6
Post-translational modification	Polyubiquitinated by RSP5.
Sequence similarities	Contains 2 RRM (RNA recognition motif) domains.

Ontologies

Keywords
Biological process	Meiosis Sporulation
Domain	Repeat
Ligand	RNA-binding
PTM	Phosphoprotein Ubl conjugation
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	premeiotic DNA replication Inferred from mutant phenotype Ref.6. Source: SGD reciprocal meiotic recombination Inferred from mutant phenotype Ref.6. Source: SGD sporulation resulting in formation of a cellular spore Inferred from genetic interaction Ref.5. Source: SGD
Cellular component	cytoplasm Inferred from direct assay. Source: SGD
Molecular function	RNA binding Inferred from mutant phenotype Ref.5. Source: SGD nucleotide binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 713	713	Meiotic activator RIM4		PRO_0000082031
Regions
	Domain	93 – 172	80	RRM 1
	Domain	346 – 420	75	RRM 2
	Compositional bias	288 – 299	12	Poly-Asn
	Compositional bias	581 – 594	14	Poly-Asn
Amino acid modifications
	Modified residue	525	1	Phosphoserine Ref.9
Experimental info
	Mutagenesis	96	1	F → L: Leads to absolute sporulation defect. Ref.5
	Mutagenesis	139	1	F → L: Leads to absolute sporulation defect. Ref.5
	Mutagenesis	349	1	F → L: Leads to mild sporulation defect. Ref.5
	Mutagenesis	385	1	F → L: Leads to absolute sporulation defect. Ref.5
	Sequence conflict	385	1	F → V in AAS56440. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	P38741 [UniParc]. Last modified February 1, 1995. Version 1. Checksum: AEB3C53530677837	FASTA	713	80,109
	10 20 30 40 50 60 MKTEISTADS LRDPPSNGLK ADSELVIRED IDQFLPSEVS SLGSDHQNDG EDSDTDSDNF 70 80 90 100 110 120 LQDPEDDVDE ESTGRGTVTT TSTSTESRGR PSSCIFVASL AAALSDDELC LSVTENFKKY 130 140 150 160 170 180 GDLARVKVLR DNANRPYAFV QYNNDHDAKH ALIRAQGTLL NGRRLRCEPA KVNRTLYLKN 190 200 210 220 230 240 QQSIDFNEIS QICEKFGGLE QIVPDRTDNQ YTRRYTYPIS SANSWFVQFV YRDDAIRAYA 250 260 270 280 290 300 NLRTDPNWII EWAQNINVPK NYNLLHKSKF KSSKYHQNNG IINNDGSNNN DNNNSNNNNR 310 320 330 340 350 360 EDSRRNGDVI EEECGHVHGS DSEEKLTSDG IYDDEDKDSE ITIDKRSIFV GQLDKETTRE 370 380 390 400 410 420 ELNRRFSTHG KIQDINLIFK PTNIFAFIKY ETEEAAAAAL ESENHAIFLN KTMHVQYKEV 430 440 450 460 470 480 GGRHNRKFSG KNGGSNFNHH QFFSTRSGKT FTGPELNLAP PPINMYRKMS GGSQQESETM 490 500 510 520 530 540 MPYMPMGPMP MGPPPPNAAS LSDFDMFPPS YSTFMKGMMP LRRKSMPNSW SSPSSKSVNS 550 560 570 580 590 600 ENESVNGGDE NSELPSEIPE SSGRYNAANS FTTYNNSSAG NSNNNNNNNN SNSNKSQYKK 610 620 630 640 650 660 RYARRSSYGY NEVPPKPYYF QPYYYHPMQY HMGPMGPLHP SQGSAGNHHP YMMVYPMSPP 670 680 690 700 710 PPSGLDGSMI PPPINVSQSH AANHGSTHVH ANEFISNDTG DINEDNKAYS LDY

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete nucleotide sequence of Saccharomyces cerevisiae chromosome VIII." Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Dover J., Du Z., Favello A., Fulton L., Gattung S., Geisel C., Kirsten J., Kucaba T., Hillier L.W., Jier M., Johnston L., Langston Y., Latreille P. , Louis E.J., Macri C., Mardis E., Menezes S., Mouser L., Nhan M., Rifkin L., Riles L., St Peter H., Trevaskis E., Vaughan K., Vignati D., Wilcox L., Wohldman P., Waterston R., Wilson R., Vaudin M. Science 265:2077-2082(1994) [PubMed: 8091229] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[3]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed: 17322287] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[4]	"Identification of functionally related genes that stimulate early meiotic gene expression in yeast." Su S.S., Mitchell A.P. Genetics 133:67-77(1993) [PubMed: 8417990] [Abstract] Cited for: FUNCTION.
[5]	"An RNA-binding protein homologue that promotes sporulation-specific gene expression in Saccharomyces cerevisiae." Soushko M., Mitchell A.P. Yeast 16:631-639(2000) [PubMed: 10806425] [Abstract] Cited for: FUNCTION, INDUCTION, MUTAGENESIS OF PHE-96; PHE-139; PHE-349 AND PHE-385.
[6]	"RIM4 encodes a meiotic activator required for early events of meiosis in Saccharomyces cerevisiae." Deng C., Saunders W.S. Mol. Genet. Genomics 266:497-504(2001) [PubMed: 11713679] [Abstract] Cited for: FUNCTION, INDUCTION.
[7]	"A high throughput screen to identify substrates for the ubiquitin ligase Rsp5." Kus B., Gajadhar A., Stanger K., Cho R., Sun W., Rouleau N., Lee T., Chan D., Wolting C., Edwards A., Bosse R., Rotin D. J. Biol. Chem. 280:29470-29478(2005) [PubMed: 15955809] [Abstract] Cited for: UBIQUITINATION BY RSP5.
[8]	"Ubiquitination screen using protein microarrays for comprehensive identification of Rsp5 substrates in yeast." Gupta R., Kus B., Fladd C., Wasmuth J., Tonikian R., Sidhu S., Krogan N.J., Parkinson J., Rotin D. Mol. Syst. Biol. 3:116-116(2007) [PubMed: 17551511] [Abstract] Cited for: UBIQUITINATION BY RSP5.
[9]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-525, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U11582 Genomic DNA. Translation: AAB65077.1. AY558114 Genomic DNA. Translation: AAS56440.1. BK006934 Genomic DNA. Translation: DAA06661.1.
PIR	S46838.
RefSeq	NP_011839.1. NM_001179104.1.
3D structure databases
ProteinModelPortal	P38741.
SMR	P38741. Positions 83-249, 345-417.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-5673N.
IntAct	P38741. 2 interactions.
MINT	MINT-497617.
STRING	P38741.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YHL024W; YHL024W; YHL024W.
GeneID	856361.
KEGG	sce:YHL024W.
NMPDR	fig|4932.3.peg.2977.
Organism-specific databases
CYGD	YHL024w.
SGD	S000001016. RIM4.
Phylogenomic databases
eggNOG	fuNOG08131.
HOGENOM	HBG203819.
OMA	FIKYETE.
OrthoDB	EOG41ZJKV.
Gene expression databases
ArrayExpress	P38741.
Genevestigator	P38741.
GermOnline	YHL024W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR012677. Nucleotide-bd_a/b_plait. IPR000504. RRM_dom. [Graphical view]
Gene3D	G3DSA:3.30.70.330. a_b_plait_nuc_bd. 2 hits.
Pfam	PF00076. RRM_1. 2 hits. [Graphical view]
SMART	SM00360. RRM. 2 hits. [Graphical view]
PROSITE	PS50102. RRM. 2 hits. [Graphical view]
ProtoNet	Search...
Other
NextBio	981820.

Entry information

Entry name

RIM4_YEAST

Accession

Primary (citable) accession number: P38741
Secondary accession number(s): D3DKU4, E9P8V3

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	February 1, 1995
Last modified:	January 25, 2012
This is version 97 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program