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Protein

Proteasome component ECM29

Gene

ECM29

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Stabilizes the proteasome holoenzyme, probably by tethering the 20S proteolytic core particle and the 19S regulatory particle. The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity.1 Publication

GO - Molecular functioni

  • protein complex scaffold Source: SGD

GO - Biological processi

  • proteasome assembly Source: SGD
Complete GO annotation...

Enzyme and pathway databases

BioCyciYEAST:G3O-31050-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Proteasome component ECM29
Alternative name(s):
Extracellular mutant protein 29
Gene namesi
Name:ECM29
Ordered Locus Names:YHL030W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VIII

Organism-specific databases

EuPathDBiFungiDB:YHL030W.
SGDiS000001022. ECM29.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: UniProtKB-SubCell
  • nucleus Source: SGD
  • proteasome complex Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus, Proteasome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedCombined sources
Chaini2 – 18681867Proteasome component ECM29PRO_0000212558Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylserineCombined sources
Modified residuei1692 – 16921PhosphoserineCombined sources

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiP38737.
PeptideAtlasiP38737.

PTM databases

iPTMnetiP38737.

Interactioni

Subunit structurei

Component of the proteasome. ECM29 binds to both proteasome 19S and 20S particles.1 Publication

GO - Molecular functioni

  • protein complex scaffold Source: SGD

Protein-protein interaction databases

BioGridi36392. 190 interactions.
DIPiDIP-6563N.
IntActiP38737. 53 interactions.
MINTiMINT-645188.

Structurei

3D structure databases

ProteinModelPortaliP38737.
SMRiP38737. Positions 1278-1308.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati32 – 6938HEAT 1Add
BLAST
Repeati116 – 15338HEAT 2Add
BLAST
Repeati256 – 29237HEAT 3Add
BLAST
Repeati313 – 35139HEAT 4Add
BLAST
Repeati384 – 42239HEAT 5Add
BLAST
Repeati439 – 47638HEAT 6Add
BLAST
Repeati590 – 62738HEAT 7Add
BLAST
Repeati707 – 74539HEAT 8Add
BLAST
Repeati748 – 78033HEAT 9Add
BLAST
Repeati781 – 81838HEAT 10Add
BLAST
Repeati846 – 88540HEAT 11Add
BLAST
Repeati945 – 98238HEAT 12Add
BLAST
Repeati988 – 102538HEAT 13Add
BLAST
Repeati1030 – 106738HEAT 14Add
BLAST
Repeati1137 – 117438HEAT 15Add
BLAST
Repeati1178 – 121538HEAT 16Add
BLAST
Repeati1272 – 131140HEAT 17Add
BLAST
Repeati1361 – 139838HEAT 18Add
BLAST
Repeati1422 – 145938HEAT 19Add
BLAST
Repeati1503 – 154038HEAT 20Add
BLAST
Repeati1544 – 158138HEAT 21Add
BLAST
Repeati1649 – 168739HEAT 22Add
BLAST
Repeati1746 – 178540HEAT 23Add
BLAST
Repeati1830 – 186738HEAT 24Add
BLAST

Sequence similaritiesi

Belongs to the ECM29 family.Curated
Contains 24 HEAT repeats.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

GeneTreeiENSGT00390000007729.
HOGENOMiHOG000248783.
InParanoidiP38737.
KOiK11886.
OMAiSGIWLFC.
OrthoDBiEOG7CZKDW.

Family and domain databases

Gene3Di1.25.10.10. 7 hits.
InterProiIPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR024372. Ecm29.
IPR026827. Ecm29/Gcn1.
[Graphical view]
PANTHERiPTHR23346. PTHR23346. 1 hit.
PTHR23346:SF19. PTHR23346:SF19. 1 hit.
PfamiPF13001. Ecm29. 1 hit.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 9 hits.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P38737-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSISSDEAKE KQLVEKAELR LAIADSPQKF ETNLQTFLPP LLLKLASPHA
60 70 80 90 100
SVRTAVFSAL KNLISRINTL PQVQLPVRAL IVQAKEPNLA AQQDSTNVRL
110 120 130 140 150
YSLLLASKGI DRLSLQDRQQ LLPLVVSNIS CLTGTVAARM FHILLKLILE
160 170 180 190 200
WVAPQESSHE QEEFVQFLQL DNDGFSFLMR QFTRFFLLVP SKQVQVSQQP
210 220 230 240 250
LSRGYTCPGL SLTDVAFFTY DAGVTFNKEQ LNKFKKAIFQ FVCRGMAATQ
260 270 280 290 300
TIEQSPRMIE LMEFLCVVST DSTNLSDDAA QFMKRFPMPY ENEEFITFLQ
310 320 330 340 350
TLYIGNTANG RPPVKAILQE KILSILNRSH FATTKAECIS LICSIGLHSS
360 370 380 390 400
EYKLRSLTLS FIRHVAKLNY KNLNPASSSP SSTDFSTCIV SLIRNNLHAE
410 420 430 440 450
GWPKLQLGPQ TPAFNTAILQ RQLQYETLGD ILKRDFELVS DLSYIEFLFE
460 470 480 490 500
SLKNDLPQFR SSIQESLLSL VGHLSILPQQ SKLKLKNLLR KNLSIDEQQR
510 520 530 540 550
EDNNDAVNSI MALKFVSIKF TNAAFPFHDP EARLFNIWGT VRTNRFDIIE
560 570 580 590 600
ESFKGLQPFW FRVNNASINT SATVKTSDLL GSHLSETEFP PFREFLQVLI
610 620 630 640 650
DQLDSEAASI TRKSLNNAVR FSKQCLISNA IYGKKTMVIQ DEDWSVRIDK
660 670 680 690 700
ALELDDTVVS RVNEMVQGMN DDIFIRYLTL LSNEFTATNS KGEQIAIFPY
710 720 730 740 750
QDPIFGSVLL TLLNFVSNNV LRRLEILVPD LYHLVIMKFQ SLSDNDLAVC
760 770 780 790 800
ATIIGIISTA IADSTHVKRI TKIAQSQTMA ETYVASYVVP RLYLKDQTNH
810 820 830 840 850
IESDSILNLL NILTTHLSHP GTNKDMILKL VCQVTKFGLL LQVSAQERKD
860 870 880 890 900
FLKKVMDTIQ DKLINDVTAI QTWSYLSLYS TDLENSSLFQ EKLLETNVSK
910 920 930 940 950
QNDFLFSVGE SLSVVAGKWS SKYLIKQIDI PNFNVEIMQQ KFPATNVTTI
960 970 980 990 1000
LDEIFSGCDS TKPSLRKASC IWLLSYIQYL GHLPEVSSKC NDIHLRFMRF
1010 1020 1030 1040 1050
LADRDEFIQD SAARGLSLVY EIGGSDLKES MVKGLLKSFT ESTAGSASTS
1060 1070 1080 1090 1100
ATGISGSVSE ETELFEPGVL NTGDGSISTY KDILNLASEV GDPALVYKFM
1110 1120 1130 1140 1150
SLAKSSALWS SRKGIAFGLG AIMSKSSLEE LLLKDQQTAK KLIPKLYRYR
1160 1170 1180 1190 1200
FDPFQAVSRS MTDIWNTLIP ESSLTISLYF NDILDELLCG MANKEWRVRE
1210 1220 1230 1240 1250
ASTSALLQLI QSQPQEKFSE KMLKIWTMAF RTMDDIKDSV REVGTKFTTV
1260 1270 1280 1290 1300
LAKILARSID VEKGVNPTKS KEILDNILPF LWGPHGLNSD AEEVRNFALT
1310 1320 1330 1340 1350
TLIDLVKHSP GAIKPFTPKL IYDFITLFSS IEPQVINYLA LNAANYNIDA
1360 1370 1380 1390 1400
NVIDTQRKNG VTNSPLFQTI EKLINNSDDC MMEEIINVVI KASRKSVGLP
1410 1420 1430 1440 1450
SKVASSLVII ILVKRYSIEM KPYSGKLLKV CLTMFEDRNE SVNIAFAISM
1460 1470 1480 1490 1500
GYLFKVSALD KCIKYSEKLI TKYFEPTSTE NNKKVVGTAI DSILNYAKSE
1510 1520 1530 1540 1550
FDNVASVFMP LIFIACNDED KDLETLYNKI WTEASSSGAG TVKLYLPEIL
1560 1570 1580 1590 1600
NVLCVNIKSN DFSIRKTCAK SVIQLCGGIN DSIPYPQIVK LFDISREALS
1610 1620 1630 1640 1650
GRSWDGKEHI VAALVSLTEK FSQTVADNND LQESINHVMY TEVSRKSMKY
1660 1670 1680 1690 1700
VKKILPLYAR YINVNPQEET ITFLIEKAKE MIRLLGSESD DSEGSIKQTS
1710 1720 1730 1740 1750
DESTIKRIKP NTEITQKSSK ENIENEEYVI NLLKVSVDIC NNSKSRYPMN
1760 1770 1780 1790 1800
LLEFIIDEIA YLFHNDRIIH TWRTQLAASE IGISIVGRFS TISSADFIQN
1810 1820 1830 1840 1850
VGRLWDQTFP INCNKETIEN VKLQMIKFGG LIIQKIPSLQ NNIEENLRLL
1860
NSIDSTSRIE LELKNIGL
Length:1,868
Mass (Da):210,431
Last modified:February 1, 1995 - v1
Checksum:iACB44C8A2FAD5C61
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U11583 Genomic DNA. Translation: AAB65042.1.
BK006934 Genomic DNA. Translation: DAA06655.1.
PIRiS48938.
RefSeqiNP_011833.1. NM_001179110.1.

Genome annotation databases

EnsemblFungiiYHL030W; YHL030W; YHL030W.
GeneIDi856355.
KEGGisce:YHL030W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U11583 Genomic DNA. Translation: AAB65042.1.
BK006934 Genomic DNA. Translation: DAA06655.1.
PIRiS48938.
RefSeqiNP_011833.1. NM_001179110.1.

3D structure databases

ProteinModelPortaliP38737.
SMRiP38737. Positions 1278-1308.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi36392. 190 interactions.
DIPiDIP-6563N.
IntActiP38737. 53 interactions.
MINTiMINT-645188.

PTM databases

iPTMnetiP38737.

Proteomic databases

MaxQBiP38737.
PeptideAtlasiP38737.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYHL030W; YHL030W; YHL030W.
GeneIDi856355.
KEGGisce:YHL030W.

Organism-specific databases

EuPathDBiFungiDB:YHL030W.
SGDiS000001022. ECM29.

Phylogenomic databases

GeneTreeiENSGT00390000007729.
HOGENOMiHOG000248783.
InParanoidiP38737.
KOiK11886.
OMAiSGIWLFC.
OrthoDBiEOG7CZKDW.

Enzyme and pathway databases

BioCyciYEAST:G3O-31050-MONOMER.

Miscellaneous databases

PROiP38737.

Family and domain databases

Gene3Di1.25.10.10. 7 hits.
InterProiIPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR024372. Ecm29.
IPR026827. Ecm29/Gcn1.
[Graphical view]
PANTHERiPTHR23346. PTHR23346. 1 hit.
PTHR23346:SF19. PTHR23346:SF19. 1 hit.
PfamiPF13001. Ecm29. 1 hit.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 9 hits.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. "Large scale identification of genes involved in cell surface biosynthesis and architecture in Saccharomyces cerevisiae."
    Lussier M., White A.-M., Sheraton J., di Paolo T., Treadwell J., Southard S.B., Horenstein C.I., Chen-Weiner J., Ram A.F.J., Kapteyn J.C., Roemer T.W., Vo D.H., Bondoc D.C., Hall J., Zhong W.-W., Sdicu A.-M., Davies J., Klis F.M., Robbins P.W., Bussey H.
    Genetics 147:435-450(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION.
  4. "Multiple associated proteins regulate proteasome structure and function."
    Leggett D.S., Hanna J., Borodovsky A., Crosas B., Schmidt M., Baker R.T., Walz T., Ploegh H., Finley D.
    Mol. Cell 10:495-507(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, IDENTIFICATION BY MASS SPECTROMETRY, IDENTIFICATION IN PROTEASOME COMPLEX.
  5. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  7. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1692, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  8. Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT SER-2, CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS], IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiECM29_YEAST
AccessioniPrimary (citable) accession number: P38737
Secondary accession number(s): D3DKT8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: June 8, 2016
This is version 128 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 2950 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome VIII
    Yeast (Saccharomyces cerevisiae) chromosome VIII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.