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Reviewed, UniProtKB/Swiss-Prot P38737 (ECM29_YEAST)

Last modified November 24, 2009. Version 71. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Proteasome component ECM29
Alternative name(s):
    Extracellular mutant protein 29
Gene names
Name: ECM29
Ordered Locus Names: YHL030W
OrganismSaccharomyces cerevisiae (Baker's yeast) [Complete proteome]
Taxonomic identifier4932 [NCBI]
Taxonomic lineageEukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

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Protein attributes

Sequence length1868 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceEvidence at protein level.

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General annotation (Comments)

Function

Stabilizes the proteasome holoenzyme, probably by tethering the 20S proteolytic core particle and the 19S regulatory particle. The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. Ref.3

Subunit structure

Component of the proteasome. ECM29 binds to both proteasome 19S and 20S particles.

Subcellular location

Cytoplasm. Nucleus.

Miscellaneous

Present with 2950 molecules/cell in log phase SD medium. Ref.5

Sequence similarities

Belongs to the ECM29 family.

Contains 24 HEAT repeats.

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Ontologies

Keywords
   Cellular componentCytoplasm
Nucleus
Proteasome
   DomainRepeat
   PTMPhosphoprotein
   Technical termComplete proteome
Gene Ontology (GO)
   Biological processprotein catabolic process

Inferred by curator. Source: SGD

   Cellular componentcytoplasm Ref.4

Inferred from direct assay. Source: SGD

nucleus Ref.4

Inferred from direct assay. Source: SGD

proteasome complex Ref.3

Inferred from direct assay. Source: SGD

   Molecular functionprotein binding

Inferred from direct assay. Source: SGD

Complete GO annotation...

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 18681868Proteasome component ECM29
PRO_0000212558

Regions

Repeat32 – 6938HEAT 1
Repeat116 – 15338HEAT 2
Repeat256 – 29237HEAT 3
Repeat313 – 35139HEAT 4
Repeat384 – 42239HEAT 5
Repeat439 – 47638HEAT 6
Repeat590 – 62738HEAT 7
Repeat707 – 74539HEAT 8
Repeat748 – 78033HEAT 9
Repeat781 – 81838HEAT 10
Repeat846 – 88540HEAT 11
Repeat945 – 98238HEAT 12
Repeat988 – 102538HEAT 13
Repeat1030 – 106738HEAT 14
Repeat1137 – 117438HEAT 15
Repeat1178 – 121538HEAT 16
Repeat1272 – 131140HEAT 17
Repeat1361 – 139838HEAT 18
Repeat1422 – 145938HEAT 19
Repeat1503 – 154038HEAT 20
Repeat1544 – 158138HEAT 21
Repeat1649 – 168739HEAT 22
Repeat1746 – 178540HEAT 23
Repeat1830 – 186738HEAT 24

Amino acid modifications

Modified residue16921Phosphoserine Ref.6
Modified residue16951Phosphoserine Ref.6

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Sequences

Sequence LengthMass (Da)Tools
P38737-1 [UniParc].

Last modified February 1, 1995. Version 1.
Checksum: ACB44C8A2FAD5C61

FASTA1,868210,431
        10         20         30         40         50         60 
MSISSDEAKE KQLVEKAELR LAIADSPQKF ETNLQTFLPP LLLKLASPHA SVRTAVFSAL 

        70         80         90        100        110        120 
KNLISRINTL PQVQLPVRAL IVQAKEPNLA AQQDSTNVRL YSLLLASKGI DRLSLQDRQQ 

       130        140        150        160        170        180 
LLPLVVSNIS CLTGTVAARM FHILLKLILE WVAPQESSHE QEEFVQFLQL DNDGFSFLMR 

       190        200        210        220        230        240 
QFTRFFLLVP SKQVQVSQQP LSRGYTCPGL SLTDVAFFTY DAGVTFNKEQ LNKFKKAIFQ 

       250        260        270        280        290        300 
FVCRGMAATQ TIEQSPRMIE LMEFLCVVST DSTNLSDDAA QFMKRFPMPY ENEEFITFLQ 

       310        320        330        340        350        360 
TLYIGNTANG RPPVKAILQE KILSILNRSH FATTKAECIS LICSIGLHSS EYKLRSLTLS 

       370        380        390        400        410        420 
FIRHVAKLNY KNLNPASSSP SSTDFSTCIV SLIRNNLHAE GWPKLQLGPQ TPAFNTAILQ 

       430        440        450        460        470        480 
RQLQYETLGD ILKRDFELVS DLSYIEFLFE SLKNDLPQFR SSIQESLLSL VGHLSILPQQ 

       490        500        510        520        530        540 
SKLKLKNLLR KNLSIDEQQR EDNNDAVNSI MALKFVSIKF TNAAFPFHDP EARLFNIWGT 

       550        560        570        580        590        600 
VRTNRFDIIE ESFKGLQPFW FRVNNASINT SATVKTSDLL GSHLSETEFP PFREFLQVLI 

       610        620        630        640        650        660 
DQLDSEAASI TRKSLNNAVR FSKQCLISNA IYGKKTMVIQ DEDWSVRIDK ALELDDTVVS 

       670        680        690        700        710        720 
RVNEMVQGMN DDIFIRYLTL LSNEFTATNS KGEQIAIFPY QDPIFGSVLL TLLNFVSNNV 

       730        740        750        760        770        780 
LRRLEILVPD LYHLVIMKFQ SLSDNDLAVC ATIIGIISTA IADSTHVKRI TKIAQSQTMA 

       790        800        810        820        830        840 
ETYVASYVVP RLYLKDQTNH IESDSILNLL NILTTHLSHP GTNKDMILKL VCQVTKFGLL 

       850        860        870        880        890        900 
LQVSAQERKD FLKKVMDTIQ DKLINDVTAI QTWSYLSLYS TDLENSSLFQ EKLLETNVSK 

       910        920        930        940        950        960 
QNDFLFSVGE SLSVVAGKWS SKYLIKQIDI PNFNVEIMQQ KFPATNVTTI LDEIFSGCDS 

       970        980        990       1000       1010       1020 
TKPSLRKASC IWLLSYIQYL GHLPEVSSKC NDIHLRFMRF LADRDEFIQD SAARGLSLVY 

      1030       1040       1050       1060       1070       1080 
EIGGSDLKES MVKGLLKSFT ESTAGSASTS ATGISGSVSE ETELFEPGVL NTGDGSISTY 

      1090       1100       1110       1120       1130       1140 
KDILNLASEV GDPALVYKFM SLAKSSALWS SRKGIAFGLG AIMSKSSLEE LLLKDQQTAK 

      1150       1160       1170       1180       1190       1200 
KLIPKLYRYR FDPFQAVSRS MTDIWNTLIP ESSLTISLYF NDILDELLCG MANKEWRVRE 

      1210       1220       1230       1240       1250       1260 
ASTSALLQLI QSQPQEKFSE KMLKIWTMAF RTMDDIKDSV REVGTKFTTV LAKILARSID 

      1270       1280       1290       1300       1310       1320 
VEKGVNPTKS KEILDNILPF LWGPHGLNSD AEEVRNFALT TLIDLVKHSP GAIKPFTPKL 

      1330       1340       1350       1360       1370       1380 
IYDFITLFSS IEPQVINYLA LNAANYNIDA NVIDTQRKNG VTNSPLFQTI EKLINNSDDC 

      1390       1400       1410       1420       1430       1440 
MMEEIINVVI KASRKSVGLP SKVASSLVII ILVKRYSIEM KPYSGKLLKV CLTMFEDRNE 

      1450       1460       1470       1480       1490       1500 
SVNIAFAISM GYLFKVSALD KCIKYSEKLI TKYFEPTSTE NNKKVVGTAI DSILNYAKSE 

      1510       1520       1530       1540       1550       1560 
FDNVASVFMP LIFIACNDED KDLETLYNKI WTEASSSGAG TVKLYLPEIL NVLCVNIKSN 

      1570       1580       1590       1600       1610       1620 
DFSIRKTCAK SVIQLCGGIN DSIPYPQIVK LFDISREALS GRSWDGKEHI VAALVSLTEK 

      1630       1640       1650       1660       1670       1680 
FSQTVADNND LQESINHVMY TEVSRKSMKY VKKILPLYAR YINVNPQEET ITFLIEKAKE 

      1690       1700       1710       1720       1730       1740 
MIRLLGSESD DSEGSIKQTS DESTIKRIKP NTEITQKSSK ENIENEEYVI NLLKVSVDIC 

      1750       1760       1770       1780       1790       1800 
NNSKSRYPMN LLEFIIDEIA YLFHNDRIIH TWRTQLAASE IGISIVGRFS TISSADFIQN 

      1810       1820       1830       1840       1850       1860 
VGRLWDQTFP INCNKETIEN VKLQMIKFGG LIIQKIPSLQ NNIEENLRLL NSIDSTSRIE 


LELKNIGL

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References

« Hide 'large scale' references
[1]"Complete nucleotide sequence of Saccharomyces cerevisiae chromosome VIII."
Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Dover J., Du Z., Favello A., Fulton L., Gattung S., Geisel C., Kirsten J., Kucaba T., Hillier L.W., Jier M., Johnston L., Langston Y., Latreille P. expand/collapse author list , Louis E.J., Macri C., Mardis E., Menezes S., Mouser L., Nhan M., Rifkin L., Riles L., St Peter H., Trevaskis E., Vaughan K., Vignati D., Wilcox L., Wohldman P., Waterston R., Wilson R., Vaudin M.
Science 265:2077-2082(1994) [PubMed: 8091229] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204511 / S288c / AB972.
[2]"Large scale identification of genes involved in cell surface biosynthesis and architecture in Saccharomyces cerevisiae."
Lussier M., White A.-M., Sheraton J., di Paolo T., Treadwell J., Southard S.B., Horenstein C.I., Chen-Weiner J., Ram A.F.J., Kapteyn J.C., Roemer T.W., Vo D.H., Bondoc D.C., Hall J., Zhong W.-W., Sdicu A.-M., Davies J., Klis F.M., Robbins P.W., Bussey H.
Genetics 147:435-450(1997) [PubMed: 9335584] [Abstract]
Cited for: IDENTIFICATION.
[3]"Multiple associated proteins regulate proteasome structure and function."
Leggett D.S., Hanna J., Borodovsky A., Crosas B., Schmidt M., Baker R.T., Walz T., Ploegh H., Finley D.
Mol. Cell 10:495-507(2002) [PubMed: 12408819] [Abstract]
Cited for: FUNCTION, MASS SPECTROMETRY, IDENTIFICATION IN PROTEASOME COMPLEX.
[4]"Global analysis of protein localization in budding yeast."
Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K.
Nature 425:686-691(2003) [PubMed: 14562095] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[5]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed: 14562106] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[6]"A multidimensional chromatography technology for in-depth phosphoproteome analysis."
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1692 AND SER-1695, MASS SPECTROMETRY.
+Additional computationally mapped references.

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Cross-references

Sequence databases

U11583 Genomic DNA. Translation: AAB65042.1.
PIRS48938.
RefSeqNP_011833.1.

3D structure databases

ModBaseSearch...

Protein-protein interaction databases

DIPDIP:6563N.
STRINGP38737.

Proteomic databases

PeptideAtlasP38737.
PRIDEP38737.

Genome annotation databases

EnsemblYHL030W; YHL030W; YHL030W; Saccharomyces cerevisiae. [Genome view]
GeneID856355.
KEGGsce:YHL030W.
NMPDRfig|4932.3.peg.2971.

Organism-specific databases

CYGDYHL030w.
SGDS000001022. ECM29.

Phylogenomic databases

HOGENOMP38737.
OMAWSSRKGI
OrthoDBEOG9HTB86

Gene expression databases

ArrayExpressP38737.
GenevestigatorP38737.
GermOnlineYHL030W. Saccharomyces cerevisiae.

Family and domain databases

InterProIPR016024. ARM-type_fold.
IPR000357. HEAT.
[Graphical view]
PfamPF02985. HEAT. 4 hits.
[Graphical view]
PROSITEPS50077. HEAT_REPEAT. False negative.
[Graphical view]
ProtoNetSearch...

Other Resources

NextBio981802.

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Entry information

Entry nameECM29_YEAST
AccessionPrimary (citable) accession number: P38737
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: November 24, 2009
This is version 71 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectFPAP (Fungal Proteome Annotation Project)

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Relevant documents

SIMILARITY comments

Index of protein domains and families

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome VIII

Yeast (Saccharomyces cerevisiae) chromosome VIII: entries and gene names

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents