Reviewed, UniProtKB/Swiss-Prot P38735 (VMR1_YEAST)

Names and origin

Protein names

Recommended name:
    ABC transporter ATP-binding protein/permease VMR1
Alternative name(s):
    Vacuolar multidrug resistance protein 1

Gene names

Name:	VMR1
Ordered Locus Names:	YHL035C

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	1592 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Subunit structure	ABC transporter which may be involved in multidrug resistance By similarity.
Subcellular location	Vacuole membrane; Multi-pass membrane protein Probable.
Induction	Under the controle of the iron homeostasis regulating AFT1 and AFT2 transcription factors. Up-regulated upon SUB2 overexpression. Ref.3 Ref.4 Ref.5
Sequence similarities	Belongs to the ABC transporter superfamily. Contains 2 ABC transmembrane type-1 domains. Contains 2 ABC transporter domains.

Ontologies

Keywords
Biological process	Transport
Cellular component	Membrane Vacuole
Domain	Repeat Transmembrane
Ligand	ATP-binding Nucleotide-binding
PTM	Glycoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell mitochondrion Inferred from direct assay. Source: SGD vacuole Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW ATPase activity, coupled to transmembrane movement of substances Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1592	1592	ABC transporter ATP-binding protein/permease VMR1		PRO_0000093465
Regions
	Topological domain	1 – 33	33	Vacuolar By similarity
	Transmembrane	34 – 54	21	1 By similarity
	Topological domain	55 – 74	20	Cytoplasmic By similarity
	Transmembrane	75 – 95	21	2 By similarity
	Topological domain	96 – 100	5	Vacuolar By similarity
	Transmembrane	101 – 121	21	3 By similarity
	Topological domain	122 – 131	10	Cytoplasmic By similarity
	Transmembrane	132 – 152	21	4 By similarity
	Topological domain	153 – 170	18	Vacuolar By similarity
	Transmembrane	171 – 191	21	5 By similarity
	Topological domain	192 – 329	138	Cytoplasmic By similarity
	Transmembrane	330 – 350	21	6 By similarity
	Topological domain	351 – 379	29	Vacuolar By similarity
	Transmembrane	380 – 400	21	7 By similarity
	Topological domain	401 – 465	65	Cytoplasmic By similarity
	Transmembrane	466 – 486	21	8 By similarity
	Topological domain	487 – 489	3	Vacuolar By similarity
	Transmembrane	490 – 510	21	9 By similarity
	Topological domain	511 – 572	62	Cytoplasmic By similarity
	Transmembrane	573 – 593	21	10 By similarity
	Topological domain	594 – 614	21	Vacuolar By similarity
	Transmembrane	615 – 635	21	11 By similarity
	Topological domain	636 – 989	354	Cytoplasmic By similarity
	Transmembrane	990 – 1010	21	12 By similarity
	Topological domain	1011 – 1051	41	Vacuolar By similarity
	Transmembrane	1052 – 1072	21	13 By similarity
	Topological domain	1073 – 1115	43	Cytoplasmic By similarity
	Transmembrane	1116 – 1136	21	14 By similarity
	Topological domain	1137	1	Vacuolar By similarity
	Transmembrane	1138 – 1158	21	15 By similarity
	Topological domain	1159 – 1229	71	Cytoplasmic By similarity
	Transmembrane	1230 – 1250	21	16 By similarity
	Topological domain	1251 – 1252	2	Vacuolar By similarity
	Transmembrane	1253 – 1273	21	17 By similarity
	Topological domain	1274 – 1592	319	Cytoplasmic By similarity
	Domain	338 – 632	295	ABC transmembrane type-1 1
	Domain	664 – 908	245	ABC transporter 1
	Domain	981 – 1282	302	ABC transmembrane type-1 2
	Domain	1323 – 1572	250	ABC transporter 2
	Nucleotide binding	702 – 709	8	ATP 1 Potential
	Nucleotide binding	1357 – 1364	8	ATP 2 Potential
Amino acid modifications
	Glycosylation	11	1	N-linked (GlcNAc...) Potential
	Glycosylation	370	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	P38735-1 [UniParc]. Last modified February 1, 1995. Version 1. Checksum: 055FB0399992ACE8	FASTA	1,592	180,926
	10 20 30 40 50 60 MGTDPLIIRN NGSFWEVDDF TRLGRTQLLS YYLPLAIIAS IGIFALCRSG LSRYVRSAEC 70 80 90 100 110 120 DLVNEYLFGA QEERKEDNSI ERLLRNSNTQ ANYVNVKKQG RILKLRHFDI TTIDVKQIDA 130 140 150 160 170 180 KNHGGLTFSR PSTSDHLRKS SEIVLMSLQI IGLSFLRVTK INIELTNRDV TTLLLFWLIL 190 200 210 220 230 240 LSLSILRVYK RSTNLWAICF TAHTTIWIST WIPIRSVYIG NIDDVPSQIF YIFEFVITST 250 260 270 280 290 300 LQPIKLTSPI KDNSSIIYVR DDHTSPSREH ISSILSCITW SWITNFIWEA QKNTIKLKDI 310 320 330 340 350 360 WGLSMEDYSI FILKGFTRRN KHINNLTLAL FESFKTYLLI GMLWVLVNSI VNLLPTILMK 370 380 390 400 410 420 RFLEIVDNPN RSSSCMNLAW LYIIGMFICR LTLAICNSQG QFVSDKICLR IRAILIGEIY 430 440 450 460 470 480 AKGLRRRLFT SPKTSSDSDS ISANLGTIIN LISIDSFKVS ELANYLYVTV QAVIMIIVVV 490 500 510 520 530 540 GLLFNFLGVS AFAGISIILV MFPLNFLLAN LLGKFQKQTL KCTDQRISKL NECLQNIRIV 550 560 570 580 590 600 KYFAWERNII NEIKSIRQKE LRSLLKKSLV WSVTSFLWFV TPTLVTGVTF AICTFVQHED 610 620 630 640 650 660 LNAPLAFTTL SLFTLLKTPL DQLSNMLSFI NQSKVSLKRI SDFLRMDDTE KYNQLTISPD 670 680 690 700 710 720 KNKIEFKNAT LTWNENDSDM NAFKLCGLNI KFQIGKLNLI LGSTGSGKSA LLLGLLGELN 730 740 750 760 770 780 LISGSIIVPS LEPKHDLIPD CEGLTNSFAY CSQSAWLLND TVKNNIIFDN FYNEDRYNKV 790 800 810 820 830 840 IDACGLKRDL EILPAGDLTE IGEKGITLSG GQKQRISLAR AVYSSAKHVL LDDCLSAVDS 850 860 870 880 890 900 HTAVWIYENC ITGPLMKNRT CILVTHNVSL TLRNAHFAIV LENGKVKNQG TITELQSKGL 910 920 930 940 950 960 FKEKYVQLSS RDSINEKNAN RLKAPRKNDS QKIEPVTENI NFDANFVNDG QLIEEEEKSN 970 980 990 1000 1010 1020 GAISPDVYKW YLKFFGGFKA LTALFALYIT AQILFISQSW WIRHWVNDTN VRINAPGFAM 1030 1040 1050 1060 1070 1080 DTLPLKGMTD SSKNKHNAFY YLTVYFLIGI IQAMLGGFKT MMTFLSGMRA SRKIFNNLLD 1090 1100 1110 1120 1130 1140 LVLHAQIRFF DVTPVGRIMN RFSKDIEGVD QELIPYLEVT IFCLIQCASI IFLITVITPR 1150 1160 1170 1180 1190 1200 FLTVAVIVFV LYFFVGKWYL TASRELKRLD SITKSPIFQH FSETLVGVCT IRAFGDERRF 1210 1220 1230 1240 1250 1260 ILENMNKIDQ NNRAFFYLSV TVKWFSFRVD MIGAFIVLAS GSFILLNIAN IDSGLAGISL 1270 1280 1290 1300 1310 1320 TYAILFTDGA LWLVRLYSTF EMNMNSVERL KEYSSIEQEN YLGHDEGRIL LLNEPSWPKD 1330 1340 1350 1360 1370 1380 GEIEIENLSL RYAPNLPPVI RNVSFKVDPQ SKIGIVGRTG AGKSTIITAL FRLLEPITGC 1390 1400 1410 1420 1430 1440 IKIDGQDISK IDLVTLRRSI TIIPQDPILF AGTIKSNVDP YDEYDEKKIF KALSQVNLIS 1450 1460 1470 1480 1490 1500 SHEFEEVLNS EERFNSTHNK FLNLHTEIAE GGLNLSQGER QLLFIARSLL REPKIILLDE 1510 1520 1530 1540 1550 1560 ATSSIDYDSD HLIQGIIRSE FNKSTILTIA HRLRSVIDYD RIIVMDAGEV KEYDRPSELL 1570 1580 1590 KDERGIFYSM CRDSGGLELL KQIAKQSSKM MK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete nucleotide sequence of Saccharomyces cerevisiae chromosome VIII." Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Dover J., Du Z., Favello A., Fulton L., Gattung S., Geisel C., Kirsten J., Kucaba T., Hillier L.W., Jier M., Johnston L., Langston Y., Latreille P. , Louis E.J., Macri C., Mardis E., Menezes S., Mouser L., Nhan M., Rifkin L., Riles L., St Peter H., Trevaskis E., Vaughan K., Vignati D., Wilcox L., Wohldman P., Waterston R., Wilson R., Vaudin M. Science 265:2077-2082(1994) [PubMed: 8091229] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	"Complete inventory of the yeast ABC proteins." Decottignies A., Goffeau A. Nat. Genet. 15:137-145(1997) [PubMed: 9020838] [Abstract] Cited for: PROTEIN FAMILY.
[3]	"Aft1p and Aft2p mediate iron-responsive gene expression in yeast through related promoter elements." Rutherford J.C., Jaron S., Winge D.R. J. Biol. Chem. 278:27636-27643(2003) [PubMed: 12756250] [Abstract] Cited for: INDUCTION.
[4]	"Gene expression profiling and phenotype analyses of S. cerevisiae in response to changing copper reveals six genes with new roles in copper and iron metabolism." van Bakel H., Strengman E., Wijmenga C., Holstege F.C.P. Physiol. Genomics 22:356-367(2005) [PubMed: 15886332] [Abstract] Cited for: INDUCTION.
[5]	"The Saccharomyces cerevisiae Sub2 protein suppresses heterochromatic silencing at telomeres and subtelomeric genes." Lahue E., Heckathorn J., Meyer Z., Smith J., Wolfe C. Yeast 22:537-551(2005) [PubMed: 15942929] [Abstract] Cited for: INDUCTION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U11583 Genomic DNA. Translation: AAB65047.1.
PIR	S48933.
RefSeq	NP_011828.1.
3D structure databases
HSSP	HSSP built from PDB template 1MT0 based on UniProtKB P08716.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:6477N.
STRING	P38735.
Proteomic databases
PRIDE	P38735.
Genome annotation databases
Ensembl	YHL035C; YHL035C; YHL035C; Saccharomyces cerevisiae. [Genome view]
GeneID	856350.
GenomeReviews	Gene locus YHL035C in contig U00093_GR.
KEGG	sce:YHL035C.
NMPDR	fig|4932.3.peg.2964.
Organism-specific databases
CYGD	YHL035c.
SGD	S000001027. VMR1.
Phylogenomic databases
HOGENOM	P38735.
OMA	LISSHEF.
Gene expression databases
ArrayExpress	P38735.
Genevestigator	P38735.
GermOnline	YHL035C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR001140. ABC_TM_transpt. IPR003439. ABC_transporter-like. IPR017871. ABC_transporter_CS. IPR017940. ABC_transporter_type1. IPR003593. ATPase_AAA+_core. [Graphical view]
Pfam	PF00664. ABC_membrane. 2 hits. PF00005. ABC_tran. 2 hits. [Graphical view]
ProDom	PD000006. ABC_transporter. 2 hits. [Graphical view] [Entries sharing at least one domain]
SMART	SM00382. AAA. 2 hits. [Graphical view]
PROSITE	PS50929. ABC_TM1F. 2 hits. PS00211. ABC_TRANSPORTER_1. 2 hits. PS50893. ABC_TRANSPORTER_2. 2 hits. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	981787.

Entry information

Entry name

VMR1_YEAST

Accession

Primary (citable) accession number: P38735

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	February 1, 1995
Last modified:	November 3, 2009
This is version 79 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)