P38732 (EFM1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
N-lysine methyltransferase EFM1
EC=2.1.1.-

Alternative name(s):
Elongation factor methyltransferase 1

Gene names

Name:	EFM1
Ordered Locus Names:	YHL039W

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	585 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Lysine methyltransferase that probably monomethylates TEF1/eEF1A at 'Lys-30' and/or 'Lys-390'. Ref.5
Subcellular location	Cytoplasm Ref.3.
Miscellaneous	Present with 5170 molecules/cell in log phase SD medium.
Sequence similarities	Contains 1 SET domain.

Ontologies

Keywords
Cellular component	Cytoplasm
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	peptidyl-lysine monomethylation Inferred from mutant phenotype Ref.5. Source: SGD
Cellular_component	cytoplasm Inferred from direct assay Ref.3. Source: SGD
Molecular_function	protein-lysine N-methyltransferase activity Inferred from mutant phenotype PubMed 22522802. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 585	585	N-lysine methyltransferase EFM1		PRO_0000202879
Regions
	Domain	8 – 287	280	SET

Sequences

	Sequence		Length	Mass (Da)
	P38732 [UniParc]. Last modified February 1, 1995. Version 1. Checksum: 84BC2D3A204D9D29	FASTA	585	67,452
	10 20 30 40 50 60 MITQTELDNC LQWAQNNGAF IDPKISFRIT EDAGVSAFVN EKFSPKPDQA LIRVPETLLI 70 80 90 100 110 120 TSQQALSEFS QAANERSLLN SVTQLYLSKL KFGTDAVHLK SFYKPYLDVL PLHLPQPYFW 130 140 150 160 170 180 STDEVMNLHG TDVYLTMRDT LNKLVKEWRM LFQALSIEHS SQDKQFLSLF QENKDSAVVP 190 200 210 220 230 240 LEQFCAHING CKLEDSEWNS FVAYLWSYCI FNSRAFPRVI LGRAGTDRTN LNEGFLYPIV 250 260 270 280 290 300 DLLNHKNDVP VRWEMNEQNE LCFMSQTTTF SAQDELFNNY GNISNEKCLL NYGFWDSSNK 310 320 330 340 350 360 FDFSRLTLKL PSTLVSGLPV DFNKSGNFVT DDGETTILQF SLKISEPLPP VLLALFAYLS 370 380 390 400 410 420 KLKSEETPTV RSVLEGIDQL TSVVSQRLLF YKNFKIKTSS TQKLRPHVIK LIKLYYQDNK 430 440 450 460 470 480 KILNATTEKL SVLQKKIYSN NKEFSLSFKT IFKNDKIFAN SLLLVFGAIN YEDLITKDCL 490 500 510 520 530 540 NDALLLWIVK LINDKSNNQG GFIKQTFKEV SDSIVIEKED VMEFLPFYKK YFPNLSERIP 550 560 570 580 EIYSVGDWGI RQFIVADTAI DRLVWIRKSN KEPIFLMKKA YDLQI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete nucleotide sequence of Saccharomyces cerevisiae chromosome VIII." Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Dover J., Du Z., Favello A., Fulton L., Gattung S., Geisel C., Kirsten J., Kucaba T., Hillier L.W., Jier M., Johnston L., Langston Y., Latreille P. , Louis E.J., Macri C., Mardis E., Menezes S., Mouser L., Nhan M., Rifkin L., Riles L., St Peter H., Trevaskis E., Vaughan K., Vignati D., Wilcox L., Wohldman P., Waterston R., Wilson R., Vaudin M. Science 265:2077-2082(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[3]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[4]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[5]	"Two novel methyltransferases acting upon eukaryotic elongation factor 1A in Saccharomyces cerevisiae." Lipson R.S., Webb K.J., Clarke S.G. Arch. Biochem. Biophys. 500:137-143(2010) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U11583 Genomic DNA. Translation: AAB65051.1. BK006934 Genomic DNA. Translation: DAA06647.1.
PIR	S48929.
RefSeq	NP_011824.1. NM_001179119.1.
3D structure databases
ProteinModelPortal	P38732.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-6582N.
IntAct	P38732. 2 interactions.
MINT	MINT-705797.
STRING	4932.YHL039W.
Proteomic databases
PaxDb	P38732.
PeptideAtlas	P38732.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YHL039W; YHL039W; YHL039W.
GeneID	856346.
KEGG	sce:YHL039W.
Organism-specific databases
CYGD	YHL039w.
SGD	S000001031. EFM1.
Phylogenomic databases
eggNOG	NOG265033.
GeneTree	ENSGT00630000090454.
HOGENOM	HOG000141878.
OMA	GELYNNY.
OrthoDB	EOG43XZCG.
Gene expression databases
Genevestigator	P38732.
GermOnline	YHL039W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR017119. Ribosomal_Lys-MeTrfase-1. [Graphical view]
PIRSF	PIRSF037136. Ribosomal_Lys-mtfrase-1. 1 hit.
PROSITE	PS50280. SET. False negative. [Graphical view]
ProtoNet	Search...
Other
NextBio	981778.

Entry information

Entry name

EFM1_YEAST

Accession

Primary (citable) accession number: P38732
Secondary accession number(s): D3DKT0

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	February 1, 1995
Last modified:	April 3, 2013
This is version 83 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program