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P38732 (EFM1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 89. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
N-lysine methyltransferase EFM1

EC=2.1.1.-
Alternative name(s):
Elongation factor methyltransferase 1
Gene names
Name:EFM1
Ordered Locus Names:YHL039W
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length585 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Lysine methyltransferase that probably monomethylates TEF1/eEF1A at 'Lys-30' and/or 'Lys-390'. Ref.5

Subcellular location

Cytoplasm Ref.3.

Miscellaneous

Present with 5170 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the class V-like SAM-binding methyltransferase superfamily.

Contains 1 SET domain.

Ontologies

Keywords
   Cellular componentCytoplasm
   LigandS-adenosyl-L-methionine
   Molecular functionMethyltransferase
Transferase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processpeptidyl-lysine monomethylation

Inferred from mutant phenotype Ref.5. Source: SGD

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionprotein-lysine N-methyltransferase activity

Inferred from mutant phenotype PubMed 22522802. Source: SGD

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 585585N-lysine methyltransferase EFM1
PRO_0000202879

Regions

Domain23 – 281259SET

Sequences

Sequence LengthMass (Da)Tools
P38732 [UniParc].

Last modified February 1, 1995. Version 1.
Checksum: 84BC2D3A204D9D29

FASTA58567,452
        10         20         30         40         50         60 
MITQTELDNC LQWAQNNGAF IDPKISFRIT EDAGVSAFVN EKFSPKPDQA LIRVPETLLI 

        70         80         90        100        110        120 
TSQQALSEFS QAANERSLLN SVTQLYLSKL KFGTDAVHLK SFYKPYLDVL PLHLPQPYFW 

       130        140        150        160        170        180 
STDEVMNLHG TDVYLTMRDT LNKLVKEWRM LFQALSIEHS SQDKQFLSLF QENKDSAVVP 

       190        200        210        220        230        240 
LEQFCAHING CKLEDSEWNS FVAYLWSYCI FNSRAFPRVI LGRAGTDRTN LNEGFLYPIV 

       250        260        270        280        290        300 
DLLNHKNDVP VRWEMNEQNE LCFMSQTTTF SAQDELFNNY GNISNEKCLL NYGFWDSSNK 

       310        320        330        340        350        360 
FDFSRLTLKL PSTLVSGLPV DFNKSGNFVT DDGETTILQF SLKISEPLPP VLLALFAYLS 

       370        380        390        400        410        420 
KLKSEETPTV RSVLEGIDQL TSVVSQRLLF YKNFKIKTSS TQKLRPHVIK LIKLYYQDNK 

       430        440        450        460        470        480 
KILNATTEKL SVLQKKIYSN NKEFSLSFKT IFKNDKIFAN SLLLVFGAIN YEDLITKDCL 

       490        500        510        520        530        540 
NDALLLWIVK LINDKSNNQG GFIKQTFKEV SDSIVIEKED VMEFLPFYKK YFPNLSERIP 

       550        560        570        580 
EIYSVGDWGI RQFIVADTAI DRLVWIRKSN KEPIFLMKKA YDLQI 

« Hide

References

« Hide 'large scale' references
[1]"Complete nucleotide sequence of Saccharomyces cerevisiae chromosome VIII."
Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Dover J., Du Z., Favello A., Fulton L., Gattung S., Geisel C., Kirsten J., Kucaba T., Hillier L.W., Jier M., Johnston L., Langston Y., Latreille P. expand/collapse author list , Louis E.J., Macri C., Mardis E., Menezes S., Mouser L., Nhan M., Rifkin L., Riles L., St Peter H., Trevaskis E., Vaughan K., Vignati D., Wilcox L., Wohldman P., Waterston R., Wilson R., Vaudin M.
Science 265:2077-2082(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[2]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[3]"Global analysis of protein localization in budding yeast."
Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K.
Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[4]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[5]"Two novel methyltransferases acting upon eukaryotic elongation factor 1A in Saccharomyces cerevisiae."
Lipson R.S., Webb K.J., Clarke S.G.
Arch. Biochem. Biophys. 500:137-143(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U11583 Genomic DNA. Translation: AAB65051.1.
BK006934 Genomic DNA. Translation: DAA06647.1.
PIRS48929.
RefSeqNP_011824.1. NM_001179119.1.

3D structure databases

ProteinModelPortalP38732.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid36384. 24 interactions.
DIPDIP-6582N.
IntActP38732. 1 interaction.
MINTMINT-705797.
STRING4932.YHL039W.

Proteomic databases

PaxDbP38732.
PeptideAtlasP38732.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYHL039W; YHL039W; YHL039W.
GeneID856346.
KEGGsce:YHL039W.

Organism-specific databases

CYGDYHL039w.
SGDS000001031. EFM1.

Phylogenomic databases

eggNOGNOG265033.
GeneTreeENSGT00630000090454.
HOGENOMHOG000141878.
OMAYNNYGDK.
OrthoDBEOG7XSTQQ.

Enzyme and pathway databases

BioCycYEAST:G3O-31057-MONOMER.

Gene expression databases

GenevestigatorP38732.

Family and domain databases

InterProIPR017119. Ribosomal_Lys-MeTrfase-1.
IPR001214. SET_dom.
[Graphical view]
PIRSFPIRSF037136. Ribosomal_Lys-mtfrase-1. 1 hit.
PROSITEPS50280. SET. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio981778.
PROP38732.

Entry information

Entry nameEFM1_YEAST
AccessionPrimary (citable) accession number: P38732
Secondary accession number(s): D3DKT0
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: February 19, 2014
This is version 89 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome VIII

Yeast (Saccharomyces cerevisiae) chromosome VIII: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families