ID   ARN1_YEAST              Reviewed;         627 AA.
AC   P38731; D3DKS9;
DT   01-FEB-1995, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1995, sequence version 1.
DT   24-JAN-2024, entry version 165.
DE   RecName: Full=Siderophore iron transporter ARN1;
DE   AltName: Full=Ferrichrome permease;
GN   Name=ARN1; OrderedLocusNames=YHL040C;
OS   Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX   NCBI_TaxID=559292;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=10744769; DOI=10.1074/jbc.275.14.10709;
RA   Yun C.-W., Ferea T., Rashford J., Ardon O., Brown P.O., Botstein D.,
RA   Kaplan J., Philpott C.C.;
RT   "Desferrioxamine-mediated iron uptake in Saccharomyces cerevisiae. Evidence
RT   for two pathways of iron uptake.";
RL   J. Biol. Chem. 275:10709-10715(2000).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=8091229; DOI=10.1126/science.8091229;
RA   Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Dover J., Du Z.,
RA   Favello A., Fulton L., Gattung S., Geisel C., Kirsten J., Kucaba T.,
RA   Hillier L.W., Jier M., Johnston L., Langston Y., Latreille P., Louis E.J.,
RA   Macri C., Mardis E., Menezes S., Mouser L., Nhan M., Rifkin L., Riles L.,
RA   St Peter H., Trevaskis E., Vaughan K., Vignati D., Wilcox L., Wohldman P.,
RA   Waterston R., Wilson R., Vaudin M.;
RT   "Complete nucleotide sequence of Saccharomyces cerevisiae chromosome
RT   VIII.";
RL   Science 265:2077-2082(1994).
RN   [3]
RP   GENOME REANNOTATION.
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=24374639; DOI=10.1534/g3.113.008995;
RA   Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA   Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA   Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT   "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL   G3 (Bethesda) 4:389-398(2014).
RN   [4]
RP   FUNCTION, AND SUBCELLULAR LOCATION.
RX   PubMed=12721368; DOI=10.1073/pnas.1030323100;
RA   Moore R.E., Kim Y., Philpott C.C.;
RT   "The mechanism of ferrichrome transport through Arn1p and its metabolism in
RT   Saccharomyces cerevisiae.";
RL   Proc. Natl. Acad. Sci. U.S.A. 100:5664-5669(2003).
RN   [5]
RP   SUBCELLULAR LOCATION.
RX   PubMed=10748025; DOI=10.1074/jbc.m001456200;
RA   Yun C.-W., Tiedeman J.S., Moore R.E., Philpott C.C.;
RT   "Siderophore-iron uptake in Saccharomyces cerevisiae. Identification of
RT   ferrichrome and fusarinine transporters.";
RL   J. Biol. Chem. 275:16354-16359(2000).
RN   [6]
RP   TOPOLOGY [LARGE SCALE ANALYSIS].
RC   STRAIN=ATCC 208353 / W303-1A;
RX   PubMed=16847258; DOI=10.1073/pnas.0604075103;
RA   Kim H., Melen K., Oesterberg M., von Heijne G.;
RT   "A global topology map of the Saccharomyces cerevisiae membrane proteome.";
RL   Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006).
CC   -!- FUNCTION: Involved in the transport of siderophore ferrichrome and so
CC       has a role in iron homeostasis. {ECO:0000269|PubMed:12721368}.
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000269|PubMed:12721368};
CC       Multi-pass membrane protein {ECO:0000269|PubMed:16847258}. Endosome
CC       membrane {ECO:0000269|PubMed:10748025}; Multi-pass membrane protein
CC       {ECO:0000269|PubMed:16847258}.
CC   -!- SIMILARITY: Belongs to the major facilitator superfamily.
CC       {ECO:0000305}.
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DR   EMBL; U11583; AAB65052.1; -; Genomic_DNA.
DR   EMBL; BK006934; DAA06646.1; -; Genomic_DNA.
DR   PIR; S48928; S48928.
DR   RefSeq; NP_011823.1; NM_001179120.1.
DR   AlphaFoldDB; P38731; -.
DR   SMR; P38731; -.
DR   BioGRID; 36383; 36.
DR   STRING; 4932.YHL040C; -.
DR   TCDB; 2.A.1.16.4; the major facilitator superfamily (mfs).
DR   PaxDb; 4932-YHL040C; -.
DR   PeptideAtlas; P38731; -.
DR   EnsemblFungi; YHL040C_mRNA; YHL040C; YHL040C.
DR   GeneID; 856345; -.
DR   KEGG; sce:YHL040C; -.
DR   AGR; SGD:S000001032; -.
DR   SGD; S000001032; ARN1.
DR   VEuPathDB; FungiDB:YHL040C; -.
DR   eggNOG; KOG0254; Eukaryota.
DR   GeneTree; ENSGT00940000176305; -.
DR   HOGENOM; CLU_012970_2_1_1; -.
DR   InParanoid; P38731; -.
DR   OMA; VPTWPFI; -.
DR   OrthoDB; 2165012at2759; -.
DR   BioCyc; YEAST:G3O-31058-MONOMER; -.
DR   BioGRID-ORCS; 856345; 0 hits in 10 CRISPR screens.
DR   PRO; PR:P38731; -.
DR   Proteomes; UP000002311; Chromosome VIII.
DR   RNAct; P38731; Protein.
DR   GO; GO:0071944; C:cell periphery; HDA:SGD.
DR   GO; GO:0005737; C:cytoplasm; HDA:SGD.
DR   GO; GO:0031410; C:cytoplasmic vesicle; IDA:SGD.
DR   GO; GO:0005768; C:endosome; IDA:SGD.
DR   GO; GO:0010008; C:endosome membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0000324; C:fungal-type vacuole; HDA:SGD.
DR   GO; GO:0005634; C:nucleus; HDA:SGD.
DR   GO; GO:0005886; C:plasma membrane; IDA:SGD.
DR   GO; GO:0005774; C:vacuolar membrane; IBA:GO_Central.
DR   GO; GO:0015343; F:siderophore-iron transmembrane transporter activity; IMP:SGD.
DR   GO; GO:0006879; P:intracellular iron ion homeostasis; IBA:GO_Central.
DR   GO; GO:0015891; P:siderophore transport; IMP:SGD.
DR   GO; GO:0055085; P:transmembrane transport; IBA:GO_Central.
DR   CDD; cd17322; MFS_ARN_like; 1.
DR   Gene3D; 1.20.1250.20; MFS general substrate transporter like domains; 2.
DR   InterPro; IPR036259; MFS_trans_sf.
DR   PANTHER; PTHR23501:SF92; GLUTATHIONE EXCHANGER 1-RELATED; 1.
DR   PANTHER; PTHR23501; MAJOR FACILITATOR SUPERFAMILY; 1.
DR   SUPFAM; SSF103473; MFS general substrate transporter; 1.
PE   1: Evidence at protein level;
KW   Cell membrane; Endosome; Ion transport; Iron; Iron transport; Membrane;
KW   Reference proteome; Transmembrane; Transmembrane helix; Transport.
FT   CHAIN           1..627
FT                   /note="Siderophore iron transporter ARN1"
FT                   /id="PRO_0000084862"
FT   TOPO_DOM        1..70
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        71..91
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        92..110
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        111..131
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        132..135
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        136..156
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        157..167
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        168..188
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        189..197
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        198..218
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        219..231
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        232..252
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        253..290
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        291..311
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        312..323
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        324..344
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        345..367
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        368..388
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        389..398
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        399..419
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        420..424
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        425..445
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        446..454
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        455..475
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        476..563
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        564..584
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        585..627
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   627 AA;  70899 MW;  FD06176163668174 CRC64;
     MESVHSRDPV KEEKKHVFMG MEHELNPETH NDSNSDSYGL PQLSEKYNAL RQNRSLIIQQ
     TEIIGSAYNK WYLQAILLLS AFICGYGYGL DGNIRYIYTG YATSSYSEHS LLSTINVINA
     VVSAASQIIY ARLSDVFGRL YLFISAVILY VVGTIIQSQA YDVQRYAAGA IFYNAGYVGV
     ILILLIILSD FSSLKWRLLY QFVPTWPFII NTWIAGNITS RANPVVNWSW DVGMWAFIFP
     LSCVPIVLCM LHMQWRARKT PEWHALKGQK SYYQEHGFIK ILKQLFWMLD VVGVLLMGCS
     LGCILVPLTL AGGVKTTWND SRLIGPFVLG FVLIPILWIW EYRFARDPIL PYRLVKDRAV
     WSSMGISFLI DFIYYMAADY LYTVMIVAVN ESVKSATRIA TLSSFVSTVA SPFFALLVTR
     CTRLKPFIMF GCALWMVAMG LLYHFRGGSQ SHSGIIGALC VWGVGTTLFT YPVTVSVQSA
     VSHENMATVT ALNYTLYRIG SAVGSAVSGA IWTQTLYKQI LKRMGDVALA TTAYESPYTF
     IETYTWGTPQ RNALMNAYKY VQRLETIVAL VFCVPLIAFS LCLRDPKLTD TVAVEYIEDG
     EYVDTKDNDP ILDWFEKLPS KFTFKRE
//