Siderophore iron transporter ARN1 - Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

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P38731 (ARN1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified May 29, 2013. Version 95. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Siderophore iron transporter ARN1

Alternative name(s):
Ferrichrome permease

Gene names

Name:	ARN1
Ordered Locus Names:	YHL040C

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces ›

Protein attributes

Sequence length	627 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Involved in the transport of siderophore ferrichrome and so has a role in iron homeostasis. Ref.4
Subcellular location	Cell membrane; Multi-pass membrane protein. Endosome membrane; Multi-pass membrane protein Ref.4 Ref.5.
Sequence similarities	Belongs to the major facilitator superfamily.

Ontologies

Keywords
Biological process	Ion transport Iron transport Transport
Cellular component	Cell membrane Endosome Membrane
Domain	Transmembrane Transmembrane helix
Ligand	Iron
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular_component	cytoplasmic membrane-bounded vesicle Inferred from direct assay Ref.5. Source: SGD endosome Inferred from direct assay PubMed 17344478. Source: SGD endosome membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from sequence model PubMed 12192589. Source: SGD nucleus Inferred from direct assay PubMed 22842922. Source: SGD plasma membrane Inferred from direct assay PubMed 15719020. Source: SGD
Molecular_function	siderophore transmembrane transporter activity Inferred from mutant phenotype PubMed 10802175. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 627

627

Siderophore iron transporter ARN1

PRO_0000084862

Regions

Topological domain

1 – 70

Extracellular Potential

Transmembrane

71 – 91

Helical; Potential

Topological domain

92 – 110

Cytoplasmic Potential

Transmembrane

111 – 131

Helical; Potential

Topological domain

132 – 135

Extracellular Potential

Transmembrane

136 – 156

Helical; Potential

Topological domain

157 – 167

Cytoplasmic Potential

Transmembrane

168 – 188

Helical; Potential

Topological domain

189 – 197

Extracellular Potential

Transmembrane

198 – 218

Helical; Potential

Topological domain

219 – 231

Cytoplasmic Potential

Transmembrane

232 – 252

Helical; Potential

Topological domain

253 – 290

Extracellular Potential

Transmembrane

291 – 311

Helical; Potential

Topological domain

312 – 323

Cytoplasmic Potential

Transmembrane

324 – 344

Helical; Potential

Topological domain

345 – 367

Extracellular Potential

Transmembrane

368 – 388

Helical; Potential

Topological domain

389 – 398

Cytoplasmic Potential

Transmembrane

399 – 419

Helical; Potential

Topological domain

420 – 424

Extracellular Potential

Transmembrane

425 – 445

Helical; Potential

Topological domain

446 – 454

Cytoplasmic Potential

Transmembrane

455 – 475

Helical; Potential

Topological domain

476 – 563

Extracellular Potential

Transmembrane

564 – 584

Helical; Potential

Topological domain

585 – 627

Cytoplasmic Potential

Sequences

Sequence

Length

Mass (Da)

Tools

P38731 [UniParc].

Last modified February 1, 1995. Version 1.
Checksum: FD06176163668174
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FASTA

627

70,899

        10         20         30         40         50         60 
MESVHSRDPV KEEKKHVFMG MEHELNPETH NDSNSDSYGL PQLSEKYNAL RQNRSLIIQQ 

        70         80         90        100        110        120 
TEIIGSAYNK WYLQAILLLS AFICGYGYGL DGNIRYIYTG YATSSYSEHS LLSTINVINA 

       130        140        150        160        170        180 
VVSAASQIIY ARLSDVFGRL YLFISAVILY VVGTIIQSQA YDVQRYAAGA IFYNAGYVGV 

       190        200        210        220        230        240 
ILILLIILSD FSSLKWRLLY QFVPTWPFII NTWIAGNITS RANPVVNWSW DVGMWAFIFP 

       250        260        270        280        290        300 
LSCVPIVLCM LHMQWRARKT PEWHALKGQK SYYQEHGFIK ILKQLFWMLD VVGVLLMGCS 

       310        320        330        340        350        360 
LGCILVPLTL AGGVKTTWND SRLIGPFVLG FVLIPILWIW EYRFARDPIL PYRLVKDRAV 

       370        380        390        400        410        420 
WSSMGISFLI DFIYYMAADY LYTVMIVAVN ESVKSATRIA TLSSFVSTVA SPFFALLVTR 

       430        440        450        460        470        480 
CTRLKPFIMF GCALWMVAMG LLYHFRGGSQ SHSGIIGALC VWGVGTTLFT YPVTVSVQSA 

       490        500        510        520        530        540 
VSHENMATVT ALNYTLYRIG SAVGSAVSGA IWTQTLYKQI LKRMGDVALA TTAYESPYTF 

       550        560        570        580        590        600 
IETYTWGTPQ RNALMNAYKY VQRLETIVAL VFCVPLIAFS LCLRDPKLTD TVAVEYIEDG 

       610        620 
EYVDTKDNDP ILDWFEKLPS KFTFKRE

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Desferrioxamine-mediated iron uptake in Saccharomyces cerevisiae. Evidence for two pathways of iron uptake." Yun C.-W., Ferea T., Rashford J., Ardon O., Brown P.O., Botstein D., Kaplan J., Philpott C.C. J. Biol. Chem. 275:10709-10715(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Complete nucleotide sequence of Saccharomyces cerevisiae chromosome VIII." Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Dover J., Du Z., Favello A., Fulton L., Gattung S., Geisel C., Kirsten J., Kucaba T., Hillier L.W., Jier M., Johnston L., Langston Y., Latreille P. Vaudin M. Science 265:2077-2082(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"The mechanism of ferrichrome transport through Arn1p and its metabolism in Saccharomyces cerevisiae." Moore R.E., Kim Y., Philpott C.C. Proc. Natl. Acad. Sci. U.S.A. 100:5664-5669(2003) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION.
[5]	"Siderophore-iron uptake in Saccharomyces cerevisiae. Identification of ferrichrome and fusarinine transporters." Yun C.-W., Tiedeman J.S., Moore R.E., Philpott C.C. J. Biol. Chem. 275:16354-16359(2000) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION.
[6]	"A global topology map of the Saccharomyces cerevisiae membrane proteome." Kim H., Melen K., Oesterberg M., von Heijne G. Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed] [Europe PMC] [Abstract] Cited for: TOPOLOGY [LARGE SCALE ANALYSIS]. Strain: ATCC 208353 / W303-1A.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U11583 Genomic DNA. Translation: AAB65052.1. BK006934 Genomic DNA. Translation: DAA06646.1.
PIR	S48928.
RefSeq	NP_011823.1. NM_001179120.1.
3D structure databases
ProteinModelPortal	P38731.
ModBase	Search...
Protein-protein interaction databases
MINT	MINT-4488358.
STRING	4932.YHL040C.
Protein family/group databases
TCDB	2.A.1.16.4. major facilitator superfamily (MFS).
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YHL040C; YHL040C; YHL040C.
GeneID	856345.
KEGG	sce:YHL040C.
Organism-specific databases
CYGD	YHL040c.
SGD	S000001032. ARN1.
Phylogenomic databases
eggNOG	NOG281153.
GeneTree	ENSGT00390000002930.
HOGENOM	HOG000181235.
KO	K08197.
OMA	TRLKPFI.
OrthoDB	EOG4XD70J.
Enzyme and pathway databases
BioCyc	YEAST:G3O-31058-MONOMER.
Gene expression databases
Genevestigator	P38731.
GermOnline	YHL040C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR016196. MFS_dom_general_subst_transpt. [Graphical view]
SUPFAM	SSF103473. MFS_gen_substrate_transporter. 1 hit.
PROSITE	PS50850. MFS. False negative. [Graphical view]
ProtoNet	Search...
Other
NextBio	981775.

Entry information

Entry name

ARN1_YEAST

Accession

Primary (citable) accession number: P38731
Secondary accession number(s): D3DKS9

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	February 1, 1995
Last modified:	May 29, 2013
This is version 95 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome VIII

Yeast (Saccharomyces cerevisiae) chromosome VIII: entries and gene names

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Protein family/group databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents