Reviewed, UniProtKB/Swiss-Prot P38731 (ARN1_YEAST)

Names and origin

Protein names

Recommended name:
    Siderophore iron transporter ARN1
Alternative name(s):
    Ferrichrome permease

Gene names

Name:	ARN1
Ordered Locus Names:	YHL040C

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	627 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Involved in the transport of siderophore ferrichrome and so has a role in iron homeostasis. Ref.3
Subcellular location	Cell membrane; Multi-pass membrane protein. Endosome membrane; Multi-pass membrane protein.
Sequence similarities	Belongs to the major facilitator superfamily.

Ontologies

Keywords
Biological process	Ion transport Iron transport Transport
Cellular component	Cell membrane Endosome Membrane
Domain	Transmembrane
Ligand	Iron
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	siderophore-iron transport Inferred from mutant phenotype. Source: SGD transmembrane transport Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasmic membrane-bounded vesicle Ref.4 Inferred from direct assay. Source: SGD endosome Traceable author statement. Source: SGD integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from direct assay. Source: SGD
Molecular function	iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW siderophore-iron transmembrane transporter activity Inferred from mutant phenotype. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 627	627	Siderophore iron transporter ARN1		PRO_0000084862
Regions
	Topological domain	1 – 70	70	Extracellular Potential
	Transmembrane	71 – 91	21	Potential
	Topological domain	92 – 110	19	Cytoplasmic Potential
	Transmembrane	111 – 131	21	Potential
	Topological domain	132 – 135	4	Extracellular Potential
	Transmembrane	136 – 156	21	Potential
	Topological domain	157 – 167	11	Cytoplasmic Potential
	Transmembrane	168 – 188	21	Potential
	Topological domain	189 – 197	9	Extracellular Potential
	Transmembrane	198 – 218	21	Potential
	Topological domain	219 – 231	13	Cytoplasmic Potential
	Transmembrane	232 – 252	21	Potential
	Topological domain	253 – 290	38	Extracellular Potential
	Transmembrane	291 – 311	21	Potential
	Topological domain	312 – 323	12	Cytoplasmic Potential
	Transmembrane	324 – 344	21	Potential
	Topological domain	345 – 367	23	Extracellular Potential
	Transmembrane	368 – 388	21	Potential
	Topological domain	389 – 398	10	Cytoplasmic Potential
	Transmembrane	399 – 419	21	Potential
	Topological domain	420 – 424	5	Extracellular Potential
	Transmembrane	425 – 445	21	Potential
	Topological domain	446 – 454	9	Cytoplasmic Potential
	Transmembrane	455 – 475	21	Potential
	Topological domain	476 – 563	88	Extracellular Potential
	Transmembrane	564 – 584	21	Potential
	Topological domain	585 – 627	43	Cytoplasmic Potential

Sequences

	Sequence		Length	Mass (Da)
	P38731-1 [UniParc]. Last modified February 1, 1995. Version 1. Checksum: FD06176163668174	FASTA	627	70,899
	10 20 30 40 50 60 MESVHSRDPV KEEKKHVFMG MEHELNPETH NDSNSDSYGL PQLSEKYNAL RQNRSLIIQQ 70 80 90 100 110 120 TEIIGSAYNK WYLQAILLLS AFICGYGYGL DGNIRYIYTG YATSSYSEHS LLSTINVINA 130 140 150 160 170 180 VVSAASQIIY ARLSDVFGRL YLFISAVILY VVGTIIQSQA YDVQRYAAGA IFYNAGYVGV 190 200 210 220 230 240 ILILLIILSD FSSLKWRLLY QFVPTWPFII NTWIAGNITS RANPVVNWSW DVGMWAFIFP 250 260 270 280 290 300 LSCVPIVLCM LHMQWRARKT PEWHALKGQK SYYQEHGFIK ILKQLFWMLD VVGVLLMGCS 310 320 330 340 350 360 LGCILVPLTL AGGVKTTWND SRLIGPFVLG FVLIPILWIW EYRFARDPIL PYRLVKDRAV 370 380 390 400 410 420 WSSMGISFLI DFIYYMAADY LYTVMIVAVN ESVKSATRIA TLSSFVSTVA SPFFALLVTR 430 440 450 460 470 480 CTRLKPFIMF GCALWMVAMG LLYHFRGGSQ SHSGIIGALC VWGVGTTLFT YPVTVSVQSA 490 500 510 520 530 540 VSHENMATVT ALNYTLYRIG SAVGSAVSGA IWTQTLYKQI LKRMGDVALA TTAYESPYTF 550 560 570 580 590 600 IETYTWGTPQ RNALMNAYKY VQRLETIVAL VFCVPLIAFS LCLRDPKLTD TVAVEYIEDG 610 620 EYVDTKDNDP ILDWFEKLPS KFTFKRE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Desferrioxamine-mediated iron uptake in Saccharomyces cerevisiae. Evidence for two pathways of iron uptake." Yun C.-W., Ferea T., Rashford J., Ardon O., Brown P.O., Botstein D., Kaplan J., Philpott C.C. J. Biol. Chem. 275:10709-10715(2000) [PubMed: 10744769] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Complete nucleotide sequence of Saccharomyces cerevisiae chromosome VIII." Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Dover J., Du Z., Favello A., Fulton L., Gattung S., Geisel C., Kirsten J., Kucaba T., Hillier L.W., Jier M., Johnston L., Langston Y., Latreille P. , Louis E.J., Macri C., Mardis E., Menezes S., Mouser L., Nhan M., Rifkin L., Riles L., St Peter H., Trevaskis E., Vaughan K., Vignati D., Wilcox L., Wohldman P., Waterston R., Wilson R., Vaudin M. Science 265:2077-2082(1994) [PubMed: 8091229] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[3]	"The mechanism of ferrichrome transport through Arn1p and its metabolism in Saccharomyces cerevisiae." Moore R.E., Kim Y., Philpott C.C. Proc. Natl. Acad. Sci. U.S.A. 100:5664-5669(2003) [PubMed: 12721368] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION.
[4]	"Siderophore-iron uptake in Saccharomyces cerevisiae. Identification of ferrichrome and fusarinine transporters." Yun C.-W., Tiedeman J.S., Moore R.E., Philpott C.C. J. Biol. Chem. 275:16354-16359(2000) [PubMed: 10748025] [Abstract] Cited for: SUBCELLULAR LOCATION.
[5]	"A global topology map of the Saccharomyces cerevisiae membrane proteome." Kim H., Melen K., Oesterberg M., von Heijne G. Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed: 16847258] [Abstract] Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U11583 Genomic DNA. Translation: AAB65052.1.
PIR	S48928.
RefSeq	NP_011823.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	P38731.
Protein family/group databases
TCDB	2.A.1.16.4. major facilitator superfamily (MFS).
Genome annotation databases
Ensembl	YHL040C; YHL040C; YHL040C; Saccharomyces cerevisiae. [Genome view]
GeneID	856345.
KEGG	sce:YHL040C.
NMPDR	fig|4932.3.peg.2959.
Organism-specific databases
CYGD	YHL040c.
SGD	S000001032. ARN1.
Phylogenomic databases
HOGENOM	P38731.
OMA	WPFIINT
OrthoDB	EOG97STWX
Gene expression databases
ArrayExpress	P38731.
Genevestigator	P38731.
GermOnline	YHL040C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR011701. MFS_1. IPR016196. MFS_general_subst_transpt. [Graphical view]
Pfam	PF07690. MFS_1. 1 hit. [Graphical view]
PROSITE	PS50850. MFS. False negative. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	981775.

Entry information

Entry name

ARN1_YEAST

Accession

Primary (citable) accession number: P38731

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	February 1, 1995
Last modified:	November 24, 2009
This is version 65 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)