P38723 (COS8_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protein COS8

Gene names

Name:	COS8
Ordered Locus Names:	YHL048W

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	381 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Subcellular location	Membrane; Multi-pass membrane protein.
Miscellaneous	Present with 3070 molecules/cell in log phase SD medium. Ref.3
Sequence similarities	Belongs to the DUP/COS family.

Ontologies

Keywords
Cellular component	Membrane
Domain	Transmembrane Transmembrane helix
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW nuclear envelope Inferred from direct assay. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
ERG11	P10614	1	EBI-4985,EBI-5127
OST1	P41543	1	EBI-4985,EBI-12651
SPF1	P39986	1	EBI-4985,EBI-3128

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 381	381	Protein COS8		PRO_0000207519
Regions
	Topological domain	1 – 42	42	Extracellular Potential
	Transmembrane	43 – 63	21	Helical; Potential
	Topological domain	64 – 72	9	Cytoplasmic Potential
	Transmembrane	73 – 93	21	Helical; Potential
	Topological domain	94 – 237	144	Extracellular Potential
	Transmembrane	238 – 258	21	Helical; Potential
	Topological domain	259 – 381	123	Cytoplasmic Potential

Sequences

	Sequence		Length	Mass (Da)
	P38723 [UniParc]. Last modified February 1, 1995. Version 1. Checksum: 6BCEDC526743F504	FASTA	381	45,880
	10 20 30 40 50 60 MKENEVKDEK SVDVLSFKQL EFQKTVLPQD VFRNELTWFC YEIYKSLAFR IWMLLWLPLS 70 80 90 100 110 120 VWWKLSSNWI HPLIVSLLVL FLGPFFVLVI CGLSRKRSLS KQLIQFCKEI TEDTPSSDPH 130 140 150 160 170 180 DWEVVAANLN SYFYENKTWN TKYFFFNAMS CQKAFKTTLL EPFSLKKDES AKVKSFKDSV 190 200 210 220 230 240 PYIEEALQVY AAGFDKEWKL FNTEKEESPF DLEDIQLPKE AYRFKLTWIL KRIFNLRCLP 250 260 270 280 290 300 LFLYYFLIVY TSGNADLISR FLFPVVMFFI MTRDFQNMRM IVLSVKMEHK MQFLSTIINE 310 320 330 340 350 360 QESGANGWDE IAKKMNRYLF EKKVWNNEEF FYDGLDCEWF FRRFFYRLLS LKKPMWFASL 370 380 NVELWPYIKE AQSARNEKPL K

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete nucleotide sequence of Saccharomyces cerevisiae chromosome VIII." Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Dover J., Du Z., Favello A., Fulton L., Gattung S., Geisel C., Kirsten J., Kucaba T., Hillier L.W., Jier M., Johnston L., Langston Y., Latreille P. , Louis E.J., Macri C., Mardis E., Menezes S., Mouser L., Nhan M., Rifkin L., Riles L., St Peter H., Trevaskis E., Vaughan K., Vignati D., Wilcox L., Wohldman P., Waterston R., Wilson R., Vaudin M. Science 265:2077-2082(1994) [PubMed: 8091229] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[3]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[4]	"A global topology map of the Saccharomyces cerevisiae membrane proteome." Kim H., Melen K., Oesterberg M., von Heijne G. Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed: 16847258] [Abstract] Cited for: TOPOLOGY [LARGE SCALE ANALYSIS]. Strain: ATCC 208353 / W303-1A.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U11583 Genomic DNA. Translation: AAB65060.1. BK006934 Genomic DNA. Translation: DAA06640.1.
PIR	S48920.
RefSeq	NP_011815.1. NM_001179128.1.
3D structure databases
ProteinModelPortal	P38723.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-4866N.
IntAct	P38723. 3 interactions.
MINT	MINT-505220.
STRING	P38723.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YHL048W; YHL048W; YHL048W.
GeneID	856337.
KEGG	sce:YHL048W.
NMPDR	fig|4932.3.peg.2950.
Organism-specific databases
CYGD	YHL048w.
SGD	S000001040. COS8.
Phylogenomic databases
HOGENOM	HBG204451.
Gene expression databases
ArrayExpress	P38723.
Genevestigator	P38723.
GermOnline	YHL048W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR001142. DUP. [Graphical view]
Pfam	PF00674. DUP. 2 hits. [Graphical view]
ProtoNet	Search...
Other
NextBio	981757.

Entry information

Entry name

COS8_YEAST

Accession

Primary (citable) accession number: P38723
Secondary accession number(s): D3DKS3

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	February 1, 1995
Last modified:	December 14, 2011
This is version 80 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program