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Protein

ATP-dependent RNA helicase DBP8

Gene

DBP8

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

ATP-binding RNA helicase involved in 40S ribosomal subunit biogenesis and is required for the normal formation of 18S rRNAs through pre-rRNA processing at A0, A1 and A2 sites. Required for vegetative growth.2 Publications

Miscellaneous

Present with 3500 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

ATP + H2O = ADP + phosphate.

pH dependencei

Optimum pH is 7.4.1 Publication

Temperature dependencei

Optimum temperature is 30 degrees Celsius.1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi46 – 53ATP8

GO - Molecular functioni

  • ATPase activity Source: SGD
  • ATP binding Source: UniProtKB-KW
  • ATP-dependent RNA helicase activity Source: SGD
  • RNA binding Source: UniProtKB-KW

GO - Biological processi

Keywordsi

Molecular functionHelicase, Hydrolase, RNA-binding
Biological processRibosome biogenesis, rRNA processing
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-31203-MONOMER
ReactomeiR-SCE-6791226 Major pathway of rRNA processing in the nucleolus and cytosol

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent RNA helicase DBP8 (EC:3.6.4.13)
Alternative name(s):
DEAD box protein 8
Gene namesi
Name:DBP8
Ordered Locus Names:YHR169W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VIII

Organism-specific databases

EuPathDBiFungiDB:YHR169W
SGDiS000001212 DBP8

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi51 – 52GK → AA in DBP8-1; loss of activity. 1 Publication2
Mutagenesisi52K → A or R: Decreases ATPase activity in vitro. 1 Publication1
Mutagenesisi155 – 156DE → AA in DBP8-2; loss of activity. 1 Publication2
Mutagenesisi155D → A: Decreases ATPase activity in vitro. 1 Publication1
Mutagenesisi188 – 190TAT → AAA in DBP8-3; severely affects growth. 1 Publication3
Mutagenesisi341 – 342RS → AA in DBP8-4; no effect on growth. 1 Publication2

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000550381 – 431ATP-dependent RNA helicase DBP8Add BLAST431

Proteomic databases

MaxQBiP38719
PaxDbiP38719
PRIDEiP38719

Interactioni

Subunit structurei

Interacts with ESF2.1 Publication

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi36603, 64 interactors
DIPiDIP-5628N
IntActiP38719, 87 interactors
MINTiP38719
STRINGi4932.YHR169W

Structurei

3D structure databases

ProteinModelPortaliP38719
SMRiP38719
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini33 – 209Helicase ATP-bindingPROSITE-ProRule annotationAdd BLAST177
Domaini242 – 389Helicase C-terminalPROSITE-ProRule annotationAdd BLAST148

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi2 – 30Q motifAdd BLAST29
Motifi155 – 158DEAD box4

Domaini

The Q motif is unique to and characteristic of the DEAD box family of RNA helicases and controls ATP binding and hydrolysis.

Sequence similaritiesi

Phylogenomic databases

GeneTreeiENSGT00730000111231
HOGENOMiHOG000268802
InParanoidiP38719
KOiK14778
OMAiISIFRFP
OrthoDBiEOG092C1VLP

Family and domain databases

CDDicd00079 HELICc, 1 hit
InterProiView protein in InterPro
IPR011545 DEAD/DEAH_box_helicase_dom
IPR014001 Helicase_ATP-bd
IPR001650 Helicase_C
IPR027417 P-loop_NTPase
IPR014014 RNA_helicase_DEAD_Q_motif
PfamiView protein in Pfam
PF00270 DEAD, 1 hit
PF00271 Helicase_C, 1 hit
SMARTiView protein in SMART
SM00487 DEXDc, 1 hit
SM00490 HELICc, 1 hit
SUPFAMiSSF52540 SSF52540, 1 hit
PROSITEiView protein in PROSITE
PS51192 HELICASE_ATP_BIND_1, 1 hit
PS51194 HELICASE_CTER, 1 hit
PS51195 Q_MOTIF, 1 hit

Sequencei

Sequence statusi: Complete.

P38719-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MADFKSLGLS KWLTESLRAM KITQPTAIQK ACIPKILEGR DCIGGAKTGS
60 70 80 90 100
GKTIAFAGPM LTKWSEDPSG MFGVVLTPTR ELAMQIAEQF TALGSSMNIR
110 120 130 140 150
VSVIVGGESI VQQALDLQRK PHFIIATPGR LAHHIMSSGD DTVGGLMRAK
160 170 180 190 200
YLVLDEADIL LTSTFADHLA TCISALPPKD KRQTLLFTAT ITDQVKSLQN
210 220 230 240 250
APVQKGKPPL FAYQVESVDN VAIPSTLKIE YILVPEHVKE AYLYQLLTCE
260 270 280 290 300
EYENKTAIIF VNRTMTAEIL RRTLKQLEVR VASLHSQMPQ QERTNSLHRF
310 320 330 340 350
RANAARILIA TDVASRGLDI PTVELVVNYD IPSDPDVFIH RSGRTARAGR
360 370 380 390 400
IGDAISFVTQ RDVSRIQAIE DRINKKMTET NKVHDTAVIR KALTKVTKAK
410 420 430
RESLMAMQKE NFGERKRQQK KKQNDGKSLR S
Length:431
Mass (Da):47,878
Last modified:February 1, 1995 - v1
Checksum:iB0451EB85247372C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00027 Genomic DNA Translation: AAB68014.1
BK006934 Genomic DNA Translation: DAA06862.1
PIRiS48908
RefSeqiNP_012039.1, NM_001179300.1

Genome annotation databases

EnsemblFungiiYHR169W; YHR169W; YHR169W
GeneIDi856574
KEGGisce:YHR169W

Similar proteinsi

Entry informationi

Entry nameiDBP8_YEAST
AccessioniPrimary (citable) accession number: P38719
Secondary accession number(s): D3DLB8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: May 23, 2018
This is version 164 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome VIII
    Yeast (Saccharomyces cerevisiae) chromosome VIII: entries and gene names

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