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P38715

- GRE3_YEAST

UniProt

P38715 - GRE3_YEAST

Protein

NADPH-dependent aldose reductase GRE3

Gene

GRE3

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 135 (01 Oct 2014)
      Sequence version 1 (01 Feb 1995)
      Previous versions | rss
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    Functioni

    Reduces the cytotoxic compound methylglyoxal (MG) to (R)-lactaldehyde similar to GRE2. MG is synthesized via a bypath of glycolysis from dihydroxyacetone phosphate and is believed to play a role in cell cycle regulation and stress adaptation. In pentose-fermenting yeasts, aldose reductase catalyzes the reduction of xylose into xylitol. The purified enzyme catalyzes this reaction, but the inability of S.cerevisiae to grow on xylose as sole carbon source indicates that the physiological function is more likely methylglyoxal reduction.

    Catalytic activityi

    Alditol + NAD(P)+ = aldose + NAD(P)H.
    (R)-lactaldehyde + NADP+ = methylglyoxal + NADPH.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei49 – 491Proton donorBy similarity
    Sitei78 – 781Lowers pKa of active site TyrBy similarity
    Binding sitei111 – 1111SubstrateBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi219 – 28668NADPBy similarityAdd
    BLAST

    GO - Molecular functioni

    1. alditol:NADP+ 1-oxidoreductase activity Source: SGD
    2. D-xylose:NADP reductase activity Source: SGD
    3. mRNA binding Source: SGD

    GO - Biological processi

    1. arabinose catabolic process Source: SGD
    2. cellular response to osmotic stress Source: SGD
    3. cellular response to oxidative stress Source: SGD
    4. D-xylose catabolic process Source: SGD
    5. galactose catabolic process Source: SGD

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Stress response

    Keywords - Ligandi

    NADP

    Enzyme and pathway databases

    BioCyciYEAST:YHR104W-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    NADPH-dependent aldose reductase GRE3 (EC:1.1.1.21)
    Alternative name(s):
    Genes de respuesta a estres protein 3
    NADPH-dependent aldo-keto reductase GRE3
    NADPH-dependent methylglyoxal reductase GRE3
    Xylose reductase (EC:1.1.1.-)
    Gene namesi
    Name:GRE3
    Ordered Locus Names:YHR104W
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome VIII

    Organism-specific databases

    CYGDiYHR104w.
    SGDiS000001146. GRE3.

    Subcellular locationi

    Cytoplasm 1 Publication. Nucleus 1 Publication

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell
    2. nucleus Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm, Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 327327NADPH-dependent aldose reductase GRE3PRO_0000124678Add
    BLAST

    Proteomic databases

    MaxQBiP38715.
    PaxDbiP38715.
    PeptideAtlasiP38715.

    Expressioni

    Inductioni

    By osmotic, ionic, oxidative and heat stress.2 Publications

    Gene expression databases

    GenevestigatoriP38715.

    Interactioni

    Protein-protein interaction databases

    BioGridi36537. 130 interactions.
    IntActiP38715. 2 interactions.
    MINTiMINT-2784158.
    STRINGi4932.YHR104W.

    Structurei

    3D structure databases

    ProteinModelPortaliP38715.
    SMRiP38715. Positions 5-318.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the aldo/keto reductase family.Curated

    Phylogenomic databases

    eggNOGiCOG0656.
    GeneTreeiENSGT00750000117547.
    HOGENOMiHOG000250272.
    KOiK17743.
    OMAiHITEAHV.
    OrthoDBiEOG7WQ83S.

    Family and domain databases

    Gene3Di3.20.20.100. 1 hit.
    InterProiIPR001395. Aldo/ket_red.
    IPR018170. Aldo/ket_reductase_CS.
    IPR020471. Aldo/keto_reductase_subgr.
    IPR023210. NADP_OxRdtase_dom.
    [Graphical view]
    PANTHERiPTHR11732. PTHR11732. 1 hit.
    PfamiPF00248. Aldo_ket_red. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000097. AKR. 1 hit.
    PRINTSiPR00069. ALDKETRDTASE.
    SUPFAMiSSF51430. SSF51430. 1 hit.
    PROSITEiPS00798. ALDOKETO_REDUCTASE_1. 1 hit.
    PS00062. ALDOKETO_REDUCTASE_2. 1 hit.
    PS00063. ALDOKETO_REDUCTASE_3. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P38715-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSSLVTLNNG LKMPLVGLGC WKIDKKVCAN QIYEAIKLGY RLFDGACDYG    50
    NEKEVGEGIR KAISEGLVSR KDIFVVSKLW NNFHHPDHVK LALKKTLSDM 100
    GLDYLDLYYI HFPIAFKYVP FEEKYPPGFY TGADDEKKGH ITEAHVPIID 150
    TYRALEECVD EGLIKSIGVS NFQGSLIQDL LRGCRIKPVA LQIEHHPYLT 200
    QEHLVEFCKL HDIQVVAYSS FGPQSFIEMD LQLAKTTPTL FENDVIKKVS 250
    QNHPGSTTSQ VLLRWATQRG IAVIPKSSKK ERLLGNLEIE KKFTLTEQEL 300
    KDISALNANI RFNDPWTWLD GKFPTFA 327
    Length:327
    Mass (Da):37,119
    Last modified:February 1, 1995 - v1
    Checksum:iAFE72B8E2DFB91C8
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U00059 Genomic DNA. Translation: AAB68858.1.
    BK006934 Genomic DNA. Translation: DAA06798.1.
    PIRiS48946.
    RefSeqiNP_011972.1. NM_001179234.1.

    Genome annotation databases

    EnsemblFungiiYHR104W; YHR104W; YHR104W.
    GeneIDi856504.
    KEGGisce:YHR104W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U00059 Genomic DNA. Translation: AAB68858.1 .
    BK006934 Genomic DNA. Translation: DAA06798.1 .
    PIRi S48946.
    RefSeqi NP_011972.1. NM_001179234.1.

    3D structure databases

    ProteinModelPortali P38715.
    SMRi P38715. Positions 5-318.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 36537. 130 interactions.
    IntActi P38715. 2 interactions.
    MINTi MINT-2784158.
    STRINGi 4932.YHR104W.

    Proteomic databases

    MaxQBi P38715.
    PaxDbi P38715.
    PeptideAtlasi P38715.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YHR104W ; YHR104W ; YHR104W .
    GeneIDi 856504.
    KEGGi sce:YHR104W.

    Organism-specific databases

    CYGDi YHR104w.
    SGDi S000001146. GRE3.

    Phylogenomic databases

    eggNOGi COG0656.
    GeneTreei ENSGT00750000117547.
    HOGENOMi HOG000250272.
    KOi K17743.
    OMAi HITEAHV.
    OrthoDBi EOG7WQ83S.

    Enzyme and pathway databases

    BioCyci YEAST:YHR104W-MONOMER.

    Miscellaneous databases

    NextBioi 982230.

    Gene expression databases

    Genevestigatori P38715.

    Family and domain databases

    Gene3Di 3.20.20.100. 1 hit.
    InterProi IPR001395. Aldo/ket_red.
    IPR018170. Aldo/ket_reductase_CS.
    IPR020471. Aldo/keto_reductase_subgr.
    IPR023210. NADP_OxRdtase_dom.
    [Graphical view ]
    PANTHERi PTHR11732. PTHR11732. 1 hit.
    Pfami PF00248. Aldo_ket_red. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000097. AKR. 1 hit.
    PRINTSi PR00069. ALDKETRDTASE.
    SUPFAMi SSF51430. SSF51430. 1 hit.
    PROSITEi PS00798. ALDOKETO_REDUCTASE_1. 1 hit.
    PS00062. ALDOKETO_REDUCTASE_2. 1 hit.
    PS00063. ALDOKETO_REDUCTASE_3. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    2. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    3. "Three genes whose expression is induced by stress in Saccharomyces cerevisiae."
      Garay-Arroyo A., Covarrubias A.A.
      Yeast 15:879-892(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: INDUCTION.
    4. "The Saccharomyces cerevisiae aldose reductase is implied in the metabolism of methylglyoxal in response to stress conditions."
      Aguilera J., Prieto J.A.
      Curr. Genet. 39:273-283(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: ACTIVITY ON METHYLGLYOXAL, INDUCTION.
    5. "Deletion of the GRE3 aldose reductase gene and its influence on xylose metabolism in recombinant strains of Saccharomyces cerevisiae expressing the xylA and XKS1 genes."
      Traff K.L., Otero Cordero R.R., van Zyl W.H., Hahn-Hagerdal B.
      Appl. Environ. Microbiol. 67:5668-5674(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: ACTIVITY ON XYLOSE.
    6. "The structure and function of yeast xylose (aldose) reductases."
      Lee H.
      Yeast 14:977-984(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: REVIEW, XYLOSE UTILIZATION.
    7. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    8. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiGRE3_YEAST
    AccessioniPrimary (citable) accession number: P38715
    Secondary accession number(s): D3DL54
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1995
    Last sequence update: February 1, 1995
    Last modified: October 1, 2014
    This is version 135 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 12851 molecules/cell in log phase SD medium.1 Publication
    'De respuesta a estres' means stress response in Spanish.

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome VIII
      Yeast (Saccharomyces cerevisiae) chromosome VIII: entries and gene names

    External Data

    Dasty 3