Reviewed, UniProtKB/Swiss-Prot P38714 (SYRM_YEAST)

Names and origin

Protein names

Recommended name:
    Arginyl-tRNA synthetase, mitochondrial
    EC=6.1.1.19
Alternative name(s):
    Arginine--tRNA ligase
      Short name=ArgRS

Gene names

Name:	MSR1
Ordered Locus Names:	YHR091C

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	643 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	ATP + L-arginine + tRNA(Arg) = AMP + diphosphate + L-arginyl-tRNA(Arg).
Subcellular location	Mitochondrion matrix.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Mitochondrion
Domain	Transit peptide
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	mitochondrial arginyl-tRNA aminoacylation Inferred by curator. Source: SGD
Cellular component	mitochondrial matrix Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW arginine-tRNA ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – ?		Mitochondrion Potential
	Chain	? – 643		Arginyl-tRNA synthetase, mitochondrial		PRO_0000035799
Regions
	Motif	188 – 198	11	"HIGH" region
Experimental info
	Sequence conflict	499	1	T → M in AAB68931. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P38714-1 [UniParc]. Last modified November 1, 1995. Version 2. Checksum: EFB4AB5D25A18CB9	FASTA	643	73,664
	10 20 30 40 50 60 MFGIVYLKNR SLLCKNPFSS YPRYGFMPSF DTQFSNQFRK LEINIGRKRY SSKTLNTKYT 70 80 90 100 110 120 DQPEGPIYPL DVLRLDISKA LHDISGIDHS LILNALESTN SMDRGDLLLP LPKIKVADPV 130 140 150 160 170 180 AVANRWAIEL STHGCIGKVC AKGPFLQFFL DQRYLIQSTV PNILLQKGKY GQKKSRHQKK 190 200 210 220 230 240 VVVEFSSPNI AKPFHAGHLR STIIGGFLSN LYEAMGWSVT RMNYLGDWGR QFGLLAVGFK 250 260 270 280 290 300 RYGDEKTLQK QPIQHLFDVY VKINMDLAKE EINGNSKCGI SGEARSFFKN LENGDENAIK 310 320 330 340 350 360 IWNRFRSLSI HHYIQTYSRL NINFDIFSGE SQVSKESMNE ALDIFRKNNL VKEIDGALVI 370 380 390 400 410 420 DLTQWSKRLG RVVVQKSDGT TLYLTRDVGA AIERKKNLHF DKMVYVISSQ QDLYMSQFFM 430 440 450 460 470 480 ILKKMNFEWA KDLQHINFGM VQGMSTRKGN VVFLDTILDE ARDKALQIME NNKMKISQVD 490 500 510 520 530 540 NPQRVADLIG VSAIIIQDTK SKRINNYEFN WNRMLSFEGD TGPYLQYTHS RLRSLERTSS 550 560 570 580 590 600 DFTTDMLIHA DFSNLNEPQL VELVRLLAQY PDVLRRAFET QEPATIVTYL FKVCHQVSSC 610 620 630 640 YKKIWVSGKP ADIAIPRLAV YSASRQVLHN AMSLLGLVPV DRM

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Yeast MSR1 gene." Tzagoloff A.A., Shtanko A. Submitted (JAN-1995) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 24657 / D273-10B.
[2]	"Complete nucleotide sequence of Saccharomyces cerevisiae chromosome VIII." Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Dover J., Du Z., Favello A., Fulton L., Gattung S., Geisel C., Kirsten J., Kucaba T., Hillier L.W., Jier M., Johnston L., Langston Y., Latreille P. , Louis E.J., Macri C., Mardis E., Menezes S., Mouser L., Nhan M., Rifkin L., Riles L., St Peter H., Trevaskis E., Vaughan K., Vignati D., Wilcox L., Wohldman P., Waterston R., Wilson R., Vaudin M. Science 265:2077-2082(1994) [PubMed: 8091229] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	L39019 Genomic DNA. Translation: AAA61486.1. U00060 Genomic DNA. Translation: AAB68931.1.
PIR	S46723.
RefSeq	NP_011959.1.
3D structure databases
HSSP	HSSP built from PDB template 1BS2 based on UniProtKB Q05506.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:1452N.
IntAct	P38714. 6 interactions.
STRING	P38714.
Genome annotation databases
Ensembl	YHR091C; YHR091C; YHR091C; Saccharomyces cerevisiae. [Genome view]
GeneID	856491.
GenomeReviews	Gene locus YHR091C in contig U00093_GR.
KEGG	sce:YHR091C.
Organism-specific databases
CYGD	YHR091c.
SGD	S000001133. MSR1.
Phylogenomic databases
HOGENOM	P38714.
Enzyme and pathway databases
BRENDA	6.1.1.19. 250.
Gene expression databases
ArrayExpress	P38714.
Genevestigator	P38714.
GermOnline	YHR091C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR001412. aa-tRNA-synth_I_CS. IPR001278. Arg-tRNA-synth_Ic. IPR015945. Arg-tRNA-synth_Ic_core. IPR005148. Arg-tRNA-synth_Ic_N. IPR008909. DALR_anticod_bd. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:3.30.1360.70. Arg-tRNA-synth_Ic_N. 1 hit. G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
PANTHER	PTHR11956. Arg_tRNA-synt_1c. 1 hit.
Pfam	PF03485. Arg_tRNA_synt_N. 1 hit. PF05746. DALR_1. 1 hit. PF00750. tRNA-synt_1d. 1 hit. [Graphical view]
PRINTS	PR01038. TRNASYNTHARG.
TIGRFAMs	TIGR00456. argS. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYRM_YEAST

Accession

Primary (citable) accession number: P38714

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	November 1, 1995
Last modified:	October 13, 2009
This is version 87 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)