P38714 (SYRM_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Arginine--tRNA ligase, mitochondrial
EC=6.1.1.19

Alternative name(s):
Arginyl-tRNA synthetase
Short name=ArgRS

Gene names

Name:	MSR1
Ordered Locus Names:	YHR091C

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	643 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + L-arginine + tRNA(Arg) = AMP + diphosphate + L-arginyl-tRNA(Arg).
Subcellular location	Mitochondrion matrix.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Mitochondrion
Domain	Transit peptide
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	mitochondrial arginyl-tRNA aminoacylation Inferred by curator PubMed 7607232. Source: SGD
Cellular_component	mitochondrial matrix Inferred from electronic annotation. Source: UniProtKB-SubCell mitochondrion Inferred from direct assay PubMed 14576278PubMed 16823961. Source: SGD
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW arginine-tRNA ligase activity Inferred from sequence alignment PubMed 7607232. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – ?		Mitochondrion Potential
	Chain	? – 643		Arginine--tRNA ligase, mitochondrial		PRO_0000035799
Regions
	Motif	188 – 198	11	"HIGH" region
Experimental info
	Sequence conflict	499	1	M → T in AAA61486. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P38714 [UniParc]. Last modified October 5, 2010. Version 3. Checksum: 7C92C0EAD5A19E0E	FASTA	643	73,694
	10 20 30 40 50 60 MFGIVYLKNR SLLCKNPFSS YPRYGFMPSF DTQFSNQFRK LEINIGRKRY SSKTLNTKYT 70 80 90 100 110 120 DQPEGPIYPL DVLRLDISKA LHDISGIDHS LILNALESTN SMDRGDLLLP LPKIKVADPV 130 140 150 160 170 180 AVANRWAIEL STHGCIGKVC AKGPFLQFFL DQRYLIQSTV PNILLQKGKY GQKKSRHQKK 190 200 210 220 230 240 VVVEFSSPNI AKPFHAGHLR STIIGGFLSN LYEAMGWSVT RMNYLGDWGR QFGLLAVGFK 250 260 270 280 290 300 RYGDEKTLQK QPIQHLFDVY VKINMDLAKE EINGNSKCGI SGEARSFFKN LENGDENAIK 310 320 330 340 350 360 IWNRFRSLSI HHYIQTYSRL NINFDIFSGE SQVSKESMNE ALDIFRKNNL VKEIDGALVI 370 380 390 400 410 420 DLTQWSKRLG RVVVQKSDGT TLYLTRDVGA AIERKKNLHF DKMVYVISSQ QDLYMSQFFM 430 440 450 460 470 480 ILKKMNFEWA KDLQHINFGM VQGMSTRKGN VVFLDTILDE ARDKALQIME NNKMKISQVD 490 500 510 520 530 540 NPQRVADLIG VSAIIIQDMK SKRINNYEFN WNRMLSFEGD TGPYLQYTHS RLRSLERTSS 550 560 570 580 590 600 DFTTDMLIHA DFSNLNEPQL VELVRLLAQY PDVLRRAFET QEPATIVTYL FKVCHQVSSC 610 620 630 640 YKKIWVSGKP ADIAIPRLAV YSASRQVLHN AMSLLGLVPV DRM

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Yeast MSR1 gene." Tzagoloff A.A., Shtanko A. Submitted (JAN-1995) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 24657 / D273-10B.
[2]	"Complete nucleotide sequence of Saccharomyces cerevisiae chromosome VIII." Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Dover J., Du Z., Favello A., Fulton L., Gattung S., Geisel C., Kirsten J., Kucaba T., Hillier L.W., Jier M., Johnston L., Langston Y., Latreille P. , Louis E.J., Macri C., Mardis E., Menezes S., Mouser L., Nhan M., Rifkin L., Riles L., St Peter H., Trevaskis E., Vaughan K., Vignati D., Wilcox L., Wohldman P., Waterston R., Wilson R., Vaudin M. Science 265:2077-2082(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L39019 Genomic DNA. Translation: AAA61486.1. U00060 Genomic DNA. Translation: AAB68931.1. BK006934 Genomic DNA. Translation: DAA06787.1.
PIR	S46723.
RefSeq	NP_011959.1. NM_001179221.1.
3D structure databases
ProteinModelPortal	P38714.
SMR	P38714. Positions 70-643.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-1452N.
IntAct	P38714. 2 interactions.
MINT	MINT-394875.
STRING	4932.YHR091C.
Proteomic databases
PaxDb	P38714.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YHR091C; YHR091C; YHR091C.
GeneID	856491.
KEGG	sce:YHR091C.
Organism-specific databases
SGD	S000001133. MSR1.
Phylogenomic databases
eggNOG	COG0018.
GeneTree	ENSGT00530000063407.
HOGENOM	HOG000247211.
KO	K01887.
OMA	KIDEEFR.
OrthoDB	EOG4FR40V.
Enzyme and pathway databases
BioCyc	YEAST:G3O-31138-MONOMER.
Gene expression databases
Genevestigator	P38714.
GermOnline	YHR091C. Saccharomyces cerevisiae.
Family and domain databases
Gene3D	3.30.1360.70. 1 hit. 3.40.50.620. 1 hit.
InterPro	IPR001412. aa-tRNA-synth_I_CS. IPR001278. Arg-tRNA-ligase_Ia. IPR015945. Arg-tRNA-synth_Ia_core. IPR005148. Arg-tRNA-synth_N. IPR008909. DALR_anticod-bd. IPR014729. Rossmann-like_a/b/a_fold. IPR009080. tRNAsynth_1a_anticodon-bd. [Graphical view]
PANTHER	PTHR11956. PTHR11956. 1 hit.
Pfam	PF03485. Arg_tRNA_synt_N. 1 hit. PF05746. DALR_1. 1 hit. PF00750. tRNA-synt_1d. 1 hit. [Graphical view]
PRINTS	PR01038. TRNASYNTHARG.
SMART	SM01016. Arg_tRNA_synt_N. 1 hit. SM00836. DALR_1. 1 hit. [Graphical view]
SUPFAM	SSF55190. Arg-tRNA-synth_Ic_N. 1 hit. SSF47323. tRNAsyn_1a_bind. 1 hit.
TIGRFAMs	TIGR00456. argS. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	982197.

Entry information

Entry name

SYRM_YEAST

Accession

Primary (citable) accession number: P38714
Secondary accession number(s): D3DL43

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	October 5, 2010
Last modified:	May 29, 2013
This is version 121 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program