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Protein

Oxysterol-binding protein homolog 3

Gene

OSH3

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

GO - Molecular functioni

  • 1-phosphatidylinositol binding Source: SGD
  • lipid binding Source: SGD
  • oxysterol binding Source: SGD
  • sterol transporter activity Source: SGD

GO - Biological processi

  • endocytosis Source: SGD
  • ER to Golgi ceramide transport Source: SGD
  • exocytosis Source: SGD
  • invasive growth in response to glucose limitation Source: SGD
  • karyogamy involved in conjugation with cellular fusion Source: SGD
  • maintenance of cell polarity Source: SGD
  • piecemeal microautophagy of nucleus Source: SGD
  • positive regulation of phosphatase activity Source: SGD
  • pseudohyphal growth Source: SGD
  • sterol transport Source: SGD
Complete GO annotation...

Keywords - Biological processi

Lipid transport, Transport

Keywords - Ligandi

Lipid-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-31122-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Oxysterol-binding protein homolog 3
Gene namesi
Name:OSH3
Ordered Locus Names:YHR073W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VIII

Organism-specific databases

EuPathDBiFungiDB:YHR073W.
SGDiS000001115. OSH3.

Subcellular locationi

GO - Cellular componenti

  • cortical endoplasmic reticulum Source: SGD
  • cytoplasm Source: SGD
  • integral component of membrane Source: InterPro
  • plasma membrane Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001003891 – 996Oxysterol-binding protein homolog 3Add BLAST996

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei190PhosphoserineCombined sources1
Modified residuei193PhosphoserineCombined sources1
Modified residuei210PhosphothreonineCombined sources1
Modified residuei323PhosphothreonineCombined sources1
Modified residuei324PhosphoserineCombined sources1
Modified residuei325PhosphothreonineCombined sources1
Modified residuei352PhosphothreonineCombined sources1
Modified residuei605PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP38713.
PRIDEiP38713.

PTM databases

iPTMnetiP38713.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
ROK1P458183EBI-12630,EBI-15686
SAC1P323682EBI-12630,EBI-16210
SCS2P400752EBI-12630,EBI-16735

Protein-protein interaction databases

BioGridi36507. 62 interactors.
DIPiDIP-2148N.
IntActiP38713. 6 interactors.
MINTiMINT-698905.

Structurei

Secondary structure

1996
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi223 – 231Combined sources9
Beta strandi240 – 247Combined sources8
Turni248 – 251Combined sources4
Beta strandi252 – 260Combined sources9
Beta strandi266 – 269Combined sources4
Helixi270 – 272Combined sources3
Beta strandi273 – 278Combined sources6
Turni279 – 282Combined sources4
Beta strandi283 – 287Combined sources5
Beta strandi292 – 296Combined sources5
Helixi300 – 311Combined sources12
Helixi312 – 314Combined sources3
Helixi643 – 649Combined sources7
Beta strandi650 – 653Combined sources4
Helixi655 – 657Combined sources3
Helixi662 – 664Combined sources3
Beta strandi665 – 669Combined sources5
Helixi670 – 675Combined sources6
Helixi676 – 678Combined sources3
Turni679 – 681Combined sources3
Helixi682 – 687Combined sources6
Helixi693 – 703Combined sources11
Helixi704 – 708Combined sources5
Helixi711 – 715Combined sources5
Beta strandi717 – 719Combined sources3
Beta strandi726 – 731Combined sources6
Helixi732 – 734Combined sources3
Beta strandi736 – 744Combined sources9
Turni745 – 748Combined sources4
Beta strandi749 – 771Combined sources23
Beta strandi773 – 788Combined sources16
Turni789 – 791Combined sources3
Beta strandi794 – 798Combined sources5
Beta strandi802 – 805Combined sources4
Beta strandi807 – 810Combined sources4
Beta strandi813 – 816Combined sources4
Beta strandi818 – 824Combined sources7
Beta strandi829 – 834Combined sources6
Beta strandi838 – 841Combined sources4
Beta strandi846 – 852Combined sources7
Beta strandi862 – 867Combined sources6
Turni868 – 870Combined sources3
Beta strandi871 – 874Combined sources4
Turni875 – 877Combined sources3
Beta strandi880 – 883Combined sources4
Helixi891 – 893Combined sources3
Turni894 – 896Combined sources3
Helixi899 – 904Combined sources6
Turni909 – 914Combined sources6
Helixi920 – 922Combined sources3
Helixi924 – 930Combined sources7
Helixi934 – 953Combined sources20
Beta strandi961 – 967Combined sources7
Beta strandi970 – 973Combined sources4
Helixi980 – 985Combined sources6

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4IAPX-ray2.30A/B221-315[»]
4IC4X-ray1.50A605-996[»]
4INQX-ray2.20A605-996[»]
ProteinModelPortaliP38713.
SMRiP38713.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini221 – 315PHPROSITE-ProRule annotationAdd BLAST95

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi514 – 520FFAT7

Domaini

The FFAT motif is required for interaction with SCS2 and proper localization of the protein.1 Publication

Sequence similaritiesi

Belongs to the OSBP family.Curated
Contains 1 PH domain.PROSITE-ProRule annotation

Phylogenomic databases

HOGENOMiHOG000193697.
InParanoidiP38713.
KOiK20463.
OMAiHGKCEAD.
OrthoDBiEOG092C0T4H.

Family and domain databases

Gene3Di2.30.29.30. 1 hit.
InterProiIPR009038. GOLD_dom.
IPR000648. Oxysterol-bd.
IPR018494. Oxysterol-bd_CS.
IPR011993. PH_dom-like.
IPR001849. PH_domain.
[Graphical view]
PANTHERiPTHR10972. PTHR10972. 4 hits.
PfamiPF01237. Oxysterol_BP. 1 hit.
[Graphical view]
SMARTiSM00233. PH. 1 hit.
[Graphical view]
SUPFAMiSSF101576. SSF101576. 2 hits.
SSF50729. SSF50729. 1 hit.
PROSITEiPS01013. OSBP. 1 hit.
PS50003. PH_DOMAIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P38713-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
METIDIQNRS FVVRWVKCGR GDVINYQIKP LKKSIEVGIY KKLKSSVDDH
60 70 80 90 100
ASAVHIAPDT KTLLDYTTKS LLHKGSSSNI EEHHRRSSQH SHSSSNGSDN
110 120 130 140 150
KRKERSYSSL SISGIQQQSQ EIPLREKLSA SGFTLVKRVG NVSGNTMVQG
160 170 180 190 200
DLEVKDTDYY YAFILDNSSS KNAKKKILFN ASVINGDNQS MISTRSTPPA
210 220 230 240 250
RPTALSRTST QQDMLFRVGQ GRYLQGYLLK KRRKRLQGFK KRFFTLDFRY
260 270 280 290 300
GTLSYYLNDH NQTCRGEIVI SLSSVSANKK DKIIIIDSGM EVWVLKATTK
310 320 330 340 350
ENWQSWVDAL QTCFDDQFED KDTSTLEENP DILDDDKEVI NKSSPQDHDH
360 370 380 390 400
LTPTATTKSA LSHRQHTQKD MDDIYVPLPS ESYATFSMNL RLIQQRLEQC
410 420 430 440 450
KKDSLSYKPT TLHQRSEGLN GTHSSSSVFT NNRVSSFNHS SSGMTSSDSL
460 470 480 490 500
ASEEVPSNKT YIEHALYNQL ADLEVFVSRF VTQGEVLFKD HQILCKKAKD
510 520 530 540 550
TRVSLTSYLS ENDEFFDAEE EISRGVIILP DTEDDINNIV EETPLLGKSD
560 570 580 590 600
QNEFTKEVQL SGSEQIASSS VESYTTNDEN HSRKHLKNRH KNRRRGHPHH
610 620 630 640 650
QKTKSAQSST ETFTSKDLFA LSYPKSVTRR NDIPEAAASP PSLLSFLRKN
660 670 680 690 700
VGKDLSSIAM PVTSNEPISI LQLISETFEY APLLTKATQR PDPITFVSAF
710 720 730 740 750
AISFLSIYRD KTRTLRKPFN PLLAETFELI REDMGFRLIS EKVSHRPPVF
760 770 780 790 800
AFFAEHLDWE CSYTVTPSQK FWGKSIELNN EGILRLKFKT TGELFEWTQP
810 820 830 840 850
TTILKNLIAG ERYMEPVNEF EVHSSKGDKS HILFDKAGMF SGRSEGFKVS
860 870 880 890 900
IIPPPSSNRK KETLAGKWTQ SLANETTHET IWEVGDLVSN PKKKYGFTKF
910 920 930 940 950
TANLNEITEI EKGNLPPTDS RLRPDIRAYE EGNVDKAEEW KLKLEQLQRE
960 970 980 990
RRNKGQDVEP KYFEKVSKNE WKYITGPKSY WERRKKHDWS DISQLW
Length:996
Mass (Da):113,760
Last modified:February 1, 1995 - v1
Checksum:iB1AD1210EFFB9AFE
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U10556 Genomic DNA. Translation: AAB68890.1.
BK006934 Genomic DNA. Translation: DAA06767.1.
PIRiS46812.
RefSeqiNP_011940.1. NM_001179203.1.

Genome annotation databases

EnsemblFungiiYHR073W; YHR073W; YHR073W.
GeneIDi856472.
KEGGisce:YHR073W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U10556 Genomic DNA. Translation: AAB68890.1.
BK006934 Genomic DNA. Translation: DAA06767.1.
PIRiS46812.
RefSeqiNP_011940.1. NM_001179203.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4IAPX-ray2.30A/B221-315[»]
4IC4X-ray1.50A605-996[»]
4INQX-ray2.20A605-996[»]
ProteinModelPortaliP38713.
SMRiP38713.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi36507. 62 interactors.
DIPiDIP-2148N.
IntActiP38713. 6 interactors.
MINTiMINT-698905.

PTM databases

iPTMnetiP38713.

Proteomic databases

MaxQBiP38713.
PRIDEiP38713.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYHR073W; YHR073W; YHR073W.
GeneIDi856472.
KEGGisce:YHR073W.

Organism-specific databases

EuPathDBiFungiDB:YHR073W.
SGDiS000001115. OSH3.

Phylogenomic databases

HOGENOMiHOG000193697.
InParanoidiP38713.
KOiK20463.
OMAiHGKCEAD.
OrthoDBiEOG092C0T4H.

Enzyme and pathway databases

BioCyciYEAST:G3O-31122-MONOMER.

Miscellaneous databases

PROiP38713.

Family and domain databases

Gene3Di2.30.29.30. 1 hit.
InterProiIPR009038. GOLD_dom.
IPR000648. Oxysterol-bd.
IPR018494. Oxysterol-bd_CS.
IPR011993. PH_dom-like.
IPR001849. PH_domain.
[Graphical view]
PANTHERiPTHR10972. PTHR10972. 4 hits.
PfamiPF01237. Oxysterol_BP. 1 hit.
[Graphical view]
SMARTiSM00233. PH. 1 hit.
[Graphical view]
SUPFAMiSSF101576. SSF101576. 2 hits.
SSF50729. SSF50729. 1 hit.
PROSITEiPS01013. OSBP. 1 hit.
PS50003. PH_DOMAIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiOSH3_YEAST
AccessioniPrimary (citable) accession number: P38713
Secondary accession number(s): D3DL23
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: November 30, 2016
This is version 147 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 589 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome VIII
    Yeast (Saccharomyces cerevisiae) chromosome VIII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.