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Protein

Oxysterol-binding protein homolog 3

Gene

OSH3

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

GO - Molecular functioni

  • 1-phosphatidylinositol binding Source: SGD
  • lipid binding Source: SGD
  • oxysterol binding Source: SGD
  • sterol transporter activity Source: SGD

GO - Biological processi

  • endocytosis Source: SGD
  • ER to Golgi ceramide transport Source: SGD
  • exocytosis Source: SGD
  • invasive growth in response to glucose limitation Source: SGD
  • karyogamy involved in conjugation with cellular fusion Source: SGD
  • maintenance of cell polarity Source: SGD
  • piecemeal microautophagy of nucleus Source: SGD
  • positive regulation of phosphatase activity Source: SGD
  • pseudohyphal growth Source: SGD
  • sterol transport Source: SGD
Complete GO annotation...

Keywords - Biological processi

Lipid transport, Transport

Keywords - Ligandi

Lipid-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-31122-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Oxysterol-binding protein homolog 3
Gene namesi
Name:OSH3
Ordered Locus Names:YHR073W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VIII

Organism-specific databases

EuPathDBiFungiDB:YHR073W.
SGDiS000001115. OSH3.

Subcellular locationi

GO - Cellular componenti

  • cortical endoplasmic reticulum Source: SGD
  • cytoplasm Source: SGD
  • integral component of membrane Source: InterPro
  • plasma membrane Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 996996Oxysterol-binding protein homolog 3PRO_0000100389Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei190 – 1901PhosphoserineCombined sources
Modified residuei193 – 1931PhosphoserineCombined sources
Modified residuei210 – 2101PhosphothreonineCombined sources
Modified residuei323 – 3231PhosphothreonineCombined sources
Modified residuei324 – 3241PhosphoserineCombined sources
Modified residuei325 – 3251PhosphothreonineCombined sources
Modified residuei352 – 3521PhosphothreonineCombined sources
Modified residuei605 – 6051PhosphoserineCombined sources

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP38713.

PTM databases

iPTMnetiP38713.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
ROK1P458183EBI-12630,EBI-15686
SAC1P323682EBI-12630,EBI-16210

Protein-protein interaction databases

BioGridi36507. 62 interactions.
DIPiDIP-2148N.
IntActiP38713. 5 interactions.
MINTiMINT-698905.

Structurei

Secondary structure

1
996
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi223 – 2319Combined sources
Beta strandi240 – 2478Combined sources
Turni248 – 2514Combined sources
Beta strandi252 – 2609Combined sources
Beta strandi266 – 2694Combined sources
Helixi270 – 2723Combined sources
Beta strandi273 – 2786Combined sources
Turni279 – 2824Combined sources
Beta strandi283 – 2875Combined sources
Beta strandi292 – 2965Combined sources
Helixi300 – 31112Combined sources
Helixi312 – 3143Combined sources
Helixi643 – 6497Combined sources
Beta strandi650 – 6534Combined sources
Helixi655 – 6573Combined sources
Helixi662 – 6643Combined sources
Beta strandi665 – 6695Combined sources
Helixi670 – 6756Combined sources
Helixi676 – 6783Combined sources
Turni679 – 6813Combined sources
Helixi682 – 6876Combined sources
Helixi693 – 70311Combined sources
Helixi704 – 7085Combined sources
Helixi711 – 7155Combined sources
Beta strandi717 – 7193Combined sources
Beta strandi726 – 7316Combined sources
Helixi732 – 7343Combined sources
Beta strandi736 – 7449Combined sources
Turni745 – 7484Combined sources
Beta strandi749 – 77123Combined sources
Beta strandi773 – 78816Combined sources
Turni789 – 7913Combined sources
Beta strandi794 – 7985Combined sources
Beta strandi802 – 8054Combined sources
Beta strandi807 – 8104Combined sources
Beta strandi813 – 8164Combined sources
Beta strandi818 – 8247Combined sources
Beta strandi829 – 8346Combined sources
Beta strandi838 – 8414Combined sources
Beta strandi846 – 8527Combined sources
Beta strandi862 – 8676Combined sources
Turni868 – 8703Combined sources
Beta strandi871 – 8744Combined sources
Turni875 – 8773Combined sources
Beta strandi880 – 8834Combined sources
Helixi891 – 8933Combined sources
Turni894 – 8963Combined sources
Helixi899 – 9046Combined sources
Turni909 – 9146Combined sources
Helixi920 – 9223Combined sources
Helixi924 – 9307Combined sources
Helixi934 – 95320Combined sources
Beta strandi961 – 9677Combined sources
Beta strandi970 – 9734Combined sources
Helixi980 – 9856Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4IAPX-ray2.30A/B221-315[»]
4IC4X-ray1.50A605-996[»]
4INQX-ray2.20A605-996[»]
ProteinModelPortaliP38713.
SMRiP38713. Positions 237-315, 625-996.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini221 – 31595PHPROSITE-ProRule annotationAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi514 – 5207FFAT

Domaini

The FFAT motif is required for interaction with SCS2 and proper localization of the protein.1 Publication

Sequence similaritiesi

Belongs to the OSBP family.Curated
Contains 1 PH domain.PROSITE-ProRule annotation

Phylogenomic databases

GeneTreeiENSGT00660000097001.
HOGENOMiHOG000193697.
InParanoidiP38713.
OMAiHGKCEAD.
OrthoDBiEOG092C0T4H.

Family and domain databases

Gene3Di2.30.29.30. 1 hit.
InterProiIPR009038. GOLD_dom.
IPR000648. Oxysterol-bd.
IPR018494. Oxysterol-bd_CS.
IPR011993. PH_dom-like.
IPR001849. PH_domain.
[Graphical view]
PANTHERiPTHR10972. PTHR10972. 4 hits.
PfamiPF01237. Oxysterol_BP. 1 hit.
[Graphical view]
SMARTiSM00233. PH. 1 hit.
[Graphical view]
SUPFAMiSSF101576. SSF101576. 2 hits.
SSF50729. SSF50729. 1 hit.
PROSITEiPS01013. OSBP. 1 hit.
PS50003. PH_DOMAIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P38713-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
METIDIQNRS FVVRWVKCGR GDVINYQIKP LKKSIEVGIY KKLKSSVDDH
60 70 80 90 100
ASAVHIAPDT KTLLDYTTKS LLHKGSSSNI EEHHRRSSQH SHSSSNGSDN
110 120 130 140 150
KRKERSYSSL SISGIQQQSQ EIPLREKLSA SGFTLVKRVG NVSGNTMVQG
160 170 180 190 200
DLEVKDTDYY YAFILDNSSS KNAKKKILFN ASVINGDNQS MISTRSTPPA
210 220 230 240 250
RPTALSRTST QQDMLFRVGQ GRYLQGYLLK KRRKRLQGFK KRFFTLDFRY
260 270 280 290 300
GTLSYYLNDH NQTCRGEIVI SLSSVSANKK DKIIIIDSGM EVWVLKATTK
310 320 330 340 350
ENWQSWVDAL QTCFDDQFED KDTSTLEENP DILDDDKEVI NKSSPQDHDH
360 370 380 390 400
LTPTATTKSA LSHRQHTQKD MDDIYVPLPS ESYATFSMNL RLIQQRLEQC
410 420 430 440 450
KKDSLSYKPT TLHQRSEGLN GTHSSSSVFT NNRVSSFNHS SSGMTSSDSL
460 470 480 490 500
ASEEVPSNKT YIEHALYNQL ADLEVFVSRF VTQGEVLFKD HQILCKKAKD
510 520 530 540 550
TRVSLTSYLS ENDEFFDAEE EISRGVIILP DTEDDINNIV EETPLLGKSD
560 570 580 590 600
QNEFTKEVQL SGSEQIASSS VESYTTNDEN HSRKHLKNRH KNRRRGHPHH
610 620 630 640 650
QKTKSAQSST ETFTSKDLFA LSYPKSVTRR NDIPEAAASP PSLLSFLRKN
660 670 680 690 700
VGKDLSSIAM PVTSNEPISI LQLISETFEY APLLTKATQR PDPITFVSAF
710 720 730 740 750
AISFLSIYRD KTRTLRKPFN PLLAETFELI REDMGFRLIS EKVSHRPPVF
760 770 780 790 800
AFFAEHLDWE CSYTVTPSQK FWGKSIELNN EGILRLKFKT TGELFEWTQP
810 820 830 840 850
TTILKNLIAG ERYMEPVNEF EVHSSKGDKS HILFDKAGMF SGRSEGFKVS
860 870 880 890 900
IIPPPSSNRK KETLAGKWTQ SLANETTHET IWEVGDLVSN PKKKYGFTKF
910 920 930 940 950
TANLNEITEI EKGNLPPTDS RLRPDIRAYE EGNVDKAEEW KLKLEQLQRE
960 970 980 990
RRNKGQDVEP KYFEKVSKNE WKYITGPKSY WERRKKHDWS DISQLW
Length:996
Mass (Da):113,760
Last modified:February 1, 1995 - v1
Checksum:iB1AD1210EFFB9AFE
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U10556 Genomic DNA. Translation: AAB68890.1.
BK006934 Genomic DNA. Translation: DAA06767.1.
PIRiS46812.
RefSeqiNP_011940.1. NM_001179203.1.

Genome annotation databases

EnsemblFungiiYHR073W; YHR073W; YHR073W.
GeneIDi856472.
KEGGisce:YHR073W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U10556 Genomic DNA. Translation: AAB68890.1.
BK006934 Genomic DNA. Translation: DAA06767.1.
PIRiS46812.
RefSeqiNP_011940.1. NM_001179203.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4IAPX-ray2.30A/B221-315[»]
4IC4X-ray1.50A605-996[»]
4INQX-ray2.20A605-996[»]
ProteinModelPortaliP38713.
SMRiP38713. Positions 237-315, 625-996.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi36507. 62 interactions.
DIPiDIP-2148N.
IntActiP38713. 5 interactions.
MINTiMINT-698905.

PTM databases

iPTMnetiP38713.

Proteomic databases

MaxQBiP38713.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYHR073W; YHR073W; YHR073W.
GeneIDi856472.
KEGGisce:YHR073W.

Organism-specific databases

EuPathDBiFungiDB:YHR073W.
SGDiS000001115. OSH3.

Phylogenomic databases

GeneTreeiENSGT00660000097001.
HOGENOMiHOG000193697.
InParanoidiP38713.
OMAiHGKCEAD.
OrthoDBiEOG092C0T4H.

Enzyme and pathway databases

BioCyciYEAST:G3O-31122-MONOMER.

Miscellaneous databases

PROiP38713.

Family and domain databases

Gene3Di2.30.29.30. 1 hit.
InterProiIPR009038. GOLD_dom.
IPR000648. Oxysterol-bd.
IPR018494. Oxysterol-bd_CS.
IPR011993. PH_dom-like.
IPR001849. PH_domain.
[Graphical view]
PANTHERiPTHR10972. PTHR10972. 4 hits.
PfamiPF01237. Oxysterol_BP. 1 hit.
[Graphical view]
SMARTiSM00233. PH. 1 hit.
[Graphical view]
SUPFAMiSSF101576. SSF101576. 2 hits.
SSF50729. SSF50729. 1 hit.
PROSITEiPS01013. OSBP. 1 hit.
PS50003. PH_DOMAIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiOSH3_YEAST
AccessioniPrimary (citable) accession number: P38713
Secondary accession number(s): D3DL23
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: September 7, 2016
This is version 144 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 589 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome VIII
    Yeast (Saccharomyces cerevisiae) chromosome VIII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.