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P38712

- RRP3_YEAST

UniProt

P38712 - RRP3_YEAST

Protein

ATP-dependent rRNA helicase RRP3

Gene

RRP3

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 127 (01 Oct 2014)
      Sequence version 2 (08 Nov 2005)
      Previous versions | rss
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    Functioni

    Required for pre-ribosomal RNA processing. Involved in the maturation of the 35S-pre-rRNA and to its cleavage to mature 18S rRNA.1 Publication

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi125 – 1328ATPPROSITE-ProRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. ATP-dependent RNA helicase activity Source: SGD
    3. RNA binding Source: UniProtKB-KW
    4. RNA-dependent ATPase activity Source: SGD

    GO - Biological processi

    1. ATP catabolic process Source: GOC
    2. maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) Source: SGD

    Keywords - Molecular functioni

    Helicase, Hydrolase

    Keywords - Biological processi

    Ribosome biogenesis, rRNA processing

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding, RNA-binding

    Enzyme and pathway databases

    BioCyciYEAST:G3O-31116-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    ATP-dependent rRNA helicase RRP3 (EC:3.6.4.-)
    Alternative name(s):
    Ribosomal RNA-processing protein 3
    Gene namesi
    Name:RRP3
    Ordered Locus Names:YHR065C
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome VIII

    Organism-specific databases

    CYGDiYHR065c.
    SGDiS000001107. RRP3.

    Subcellular locationi

    Nucleus Curated

    GO - Cellular componenti

    1. nucleolus Source: SGD

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 501501ATP-dependent rRNA helicase RRP3PRO_0000055064Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei43 – 431Phosphoserine2 Publications
    Modified residuei45 – 451Phosphoserine2 Publications
    Modified residuei47 – 471Phosphoserine2 Publications

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiP38712.
    PaxDbiP38712.
    PeptideAtlasiP38712.

    Expressioni

    Gene expression databases

    GenevestigatoriP38712.

    Interactioni

    Protein-protein interaction databases

    BioGridi36497. 15 interactions.
    DIPiDIP-6533N.
    IntActiP38712. 1 interaction.
    MINTiMINT-518716.

    Structurei

    3D structure databases

    ProteinModelPortaliP38712.
    SMRiP38712. Positions 76-446.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini112 – 284173Helicase ATP-bindingPROSITE-ProRule annotationAdd
    BLAST
    Domaini307 – 461155Helicase C-terminalPROSITE-ProRule annotationAdd
    BLAST

    Coiled coil

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Coiled coili3 – 4442Sequence AnalysisAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi81 – 10929Q motifAdd
    BLAST
    Motifi231 – 2344DEAD box

    Domaini

    The Q motif is unique to and characteristic of the DEAD box family of RNA helicases and controls ATP binding and hydrolysis.

    Sequence similaritiesi

    Contains 1 helicase ATP-binding domain.PROSITE-ProRule annotation
    Contains 1 helicase C-terminal domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Coiled coil

    Phylogenomic databases

    eggNOGiCOG0513.
    GeneTreeiENSGT00730000110914.
    HOGENOMiHOG000268802.
    KOiK14777.
    OMAiNKFRAGS.
    OrthoDBiEOG7FR7R5.

    Family and domain databases

    Gene3Di3.40.50.300. 2 hits.
    InterProiIPR011545. DNA/RNA_helicase_DEAD/DEAH_N.
    IPR014001. Helicase_ATP-bd.
    IPR001650. Helicase_C.
    IPR027417. P-loop_NTPase.
    IPR000629. RNA-helicase_DEAD-box_CS.
    IPR014014. RNA_helicase_DEAD_Q_motif.
    [Graphical view]
    PfamiPF00270. DEAD. 1 hit.
    PF00271. Helicase_C. 1 hit.
    [Graphical view]
    SMARTiSM00487. DEXDc. 1 hit.
    SM00490. HELICc. 1 hit.
    [Graphical view]
    SUPFAMiSSF52540. SSF52540. 1 hit.
    PROSITEiPS00039. DEAD_ATP_HELICASE. 1 hit.
    PS51192. HELICASE_ATP_BIND_1. 1 hit.
    PS51194. HELICASE_CTER. 1 hit.
    PS51195. Q_MOTIF. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P38712-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSKIVKRKEK KANDELTSLA EKIRAKALEN QKKLIEAEKE GGSESDSEED    50
    ATAEKKKVLK SKSKSTVSTQ NENTNEDESF ESFSELNLVP ELIQACKNLN 100
    YSKPTPIQSK AIPPALEGHD IIGLAQTGSG KTAAFAIPIL NRLWHDQEPY 150
    YACILAPTRE LAQQIKETFD SLGSLMGVRS TCIVGGMNMM DQARDLMRKP 200
    HIIIATPGRL MDHLENTKGF SLRKLKFLVM DEADRLLDME FGPVLDRILK 250
    IIPTQERTTY LFSATMTSKI DKLQRASLTN PVKCAVSNKY QTVDTLVQTL 300
    MVVPGGLKNT YLIYLLNEFI GKTMIIFTRT KANAERLSGL CNLLEFSATA 350
    LHGDLNQNQR MGSLDLFKAG KRSILVATDV AARGLDIPSV DIVVNYDIPV 400
    DSKSYIHRVG RTARAGRSGK SISLVSQYDL ELILRIEEVL GKKLPKESVD 450
    KNIILTLRDS VDKANGEVVM EMNRRNKEKI ARGKGRRGRM MTRENMDMGE 500
    R 501
    Length:501
    Mass (Da):55,969
    Last modified:November 8, 2005 - v2
    Checksum:iAA17798E0897272F
    GO

    Sequence cautioni

    The sequence AAB68392.1 differs from that shown. Reason: Erroneous initiation.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U00061 Genomic DNA. Translation: AAB68392.1. Different initiation.
    AY389302 mRNA. Translation: AAQ97234.1.
    AY389303 mRNA. Translation: AAQ97235.1.
    BK006934 Genomic DNA. Translation: DAA06758.1.
    PIRiS46713.
    RefSeqiNP_011932.2. NM_001179195.1.

    Genome annotation databases

    EnsemblFungiiYHR065C; YHR065C; YHR065C.
    GeneIDi856462.
    KEGGisce:YHR065C.

    Cross-referencesi

    Web resourcesi

    RNA helicases from the baker's yeast Saccharomyces cerevisiae

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U00061 Genomic DNA. Translation: AAB68392.1 . Different initiation.
    AY389302 mRNA. Translation: AAQ97234.1 .
    AY389303 mRNA. Translation: AAQ97235.1 .
    BK006934 Genomic DNA. Translation: DAA06758.1 .
    PIRi S46713.
    RefSeqi NP_011932.2. NM_001179195.1.

    3D structure databases

    ProteinModelPortali P38712.
    SMRi P38712. Positions 76-446.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 36497. 15 interactions.
    DIPi DIP-6533N.
    IntActi P38712. 1 interaction.
    MINTi MINT-518716.

    Proteomic databases

    MaxQBi P38712.
    PaxDbi P38712.
    PeptideAtlasi P38712.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YHR065C ; YHR065C ; YHR065C .
    GeneIDi 856462.
    KEGGi sce:YHR065C.

    Organism-specific databases

    CYGDi YHR065c.
    SGDi S000001107. RRP3.

    Phylogenomic databases

    eggNOGi COG0513.
    GeneTreei ENSGT00730000110914.
    HOGENOMi HOG000268802.
    KOi K14777.
    OMAi NKFRAGS.
    OrthoDBi EOG7FR7R5.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-31116-MONOMER.

    Miscellaneous databases

    NextBioi 982111.
    PROi P38712.

    Gene expression databases

    Genevestigatori P38712.

    Family and domain databases

    Gene3Di 3.40.50.300. 2 hits.
    InterProi IPR011545. DNA/RNA_helicase_DEAD/DEAH_N.
    IPR014001. Helicase_ATP-bd.
    IPR001650. Helicase_C.
    IPR027417. P-loop_NTPase.
    IPR000629. RNA-helicase_DEAD-box_CS.
    IPR014014. RNA_helicase_DEAD_Q_motif.
    [Graphical view ]
    Pfami PF00270. DEAD. 1 hit.
    PF00271. Helicase_C. 1 hit.
    [Graphical view ]
    SMARTi SM00487. DEXDc. 1 hit.
    SM00490. HELICc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52540. SSF52540. 1 hit.
    PROSITEi PS00039. DEAD_ATP_HELICASE. 1 hit.
    PS51192. HELICASE_ATP_BIND_1. 1 hit.
    PS51194. HELICASE_CTER. 1 hit.
    PS51195. Q_MOTIF. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    2. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    3. "Verification of 3' and 5' ends of S.cerevisiae transcripts."
      Kennedy M.C., Dietrich F.S.
      Submitted (SEP-2003) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-72 AND 478-501.
      Strain: ATCC 204511 / S288c / AB972.
    4. "18S rRNA processing requires the RNA helicase-like protein Rrp3."
      O'Day C.L., Chavanikamannil F., Abelson J.
      Nucleic Acids Res. 24:3201-3207(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    5. "Sequencing and comparison of yeast species to identify genes and regulatory elements."
      Kellis M., Patterson N., Endrizzi M., Birren B.W., Lander E.S.
      Nature 423:241-254(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION OF PROBABLE INITIATION SITE.
    6. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
      Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
      J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-43; SER-45 AND SER-47, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Strain: ADR376.
    7. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
      Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
      Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    8. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
      Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
      Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-43; SER-45 AND SER-47, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiRRP3_YEAST
    AccessioniPrimary (citable) accession number: P38712
    Secondary accession number(s): D3DL14, Q6TQT5, Q6TQT6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1995
    Last sequence update: November 8, 2005
    Last modified: October 1, 2014
    This is version 127 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome VIII
      Yeast (Saccharomyces cerevisiae) chromosome VIII: entries and gene names

    External Data

    Dasty 3