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P38712 (RRP3_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 124. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Web links·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
ATP-dependent rRNA helicase RRP3

EC=3.6.4.-
Alternative name(s):
Ribosomal RNA-processing protein 3
Gene names
Name:RRP3
Ordered Locus Names:YHR065C
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length501 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Required for pre-ribosomal RNA processing. Involved in the maturation of the 35S-pre-rRNA and to its cleavage to mature 18S rRNA. Ref.4

Subcellular location

Nucleus Probable.

Domain

The Q motif is unique to and characteristic of the DEAD box family of RNA helicases and controls ATP binding and hydrolysis.

Sequence similarities

Belongs to the DEAD box helicase family. DDX47/RRP3 subfamily.

Contains 1 helicase ATP-binding domain.

Contains 1 helicase C-terminal domain.

Sequence caution

The sequence AAB68392.1 differs from that shown. Reason: Erroneous initiation.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 501501ATP-dependent rRNA helicase RRP3
PRO_0000055064

Regions

Domain112 – 284173Helicase ATP-binding
Domain307 – 461155Helicase C-terminal
Nucleotide binding125 – 1328ATP By similarity
Coiled coil3 – 4442 Potential
Motif81 – 10929Q motif
Motif231 – 2344DEAD box

Amino acid modifications

Modified residue431Phosphoserine Ref.6 Ref.8
Modified residue451Phosphoserine Ref.6 Ref.8
Modified residue471Phosphoserine Ref.6 Ref.8

Sequences

Sequence LengthMass (Da)Tools
P38712 [UniParc].

Last modified November 8, 2005. Version 2.
Checksum: AA17798E0897272F

FASTA50155,969
        10         20         30         40         50         60 
MSKIVKRKEK KANDELTSLA EKIRAKALEN QKKLIEAEKE GGSESDSEED ATAEKKKVLK 

        70         80         90        100        110        120 
SKSKSTVSTQ NENTNEDESF ESFSELNLVP ELIQACKNLN YSKPTPIQSK AIPPALEGHD 

       130        140        150        160        170        180 
IIGLAQTGSG KTAAFAIPIL NRLWHDQEPY YACILAPTRE LAQQIKETFD SLGSLMGVRS 

       190        200        210        220        230        240 
TCIVGGMNMM DQARDLMRKP HIIIATPGRL MDHLENTKGF SLRKLKFLVM DEADRLLDME 

       250        260        270        280        290        300 
FGPVLDRILK IIPTQERTTY LFSATMTSKI DKLQRASLTN PVKCAVSNKY QTVDTLVQTL 

       310        320        330        340        350        360 
MVVPGGLKNT YLIYLLNEFI GKTMIIFTRT KANAERLSGL CNLLEFSATA LHGDLNQNQR 

       370        380        390        400        410        420 
MGSLDLFKAG KRSILVATDV AARGLDIPSV DIVVNYDIPV DSKSYIHRVG RTARAGRSGK 

       430        440        450        460        470        480 
SISLVSQYDL ELILRIEEVL GKKLPKESVD KNIILTLRDS VDKANGEVVM EMNRRNKEKI 

       490        500 
ARGKGRRGRM MTRENMDMGE R 

« Hide

References

« Hide 'large scale' references
[1]"Complete nucleotide sequence of Saccharomyces cerevisiae chromosome VIII."
Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Dover J., Du Z., Favello A., Fulton L., Gattung S., Geisel C., Kirsten J., Kucaba T., Hillier L.W., Jier M., Johnston L., Langston Y., Latreille P. expand/collapse author list , Louis E.J., Macri C., Mardis E., Menezes S., Mouser L., Nhan M., Rifkin L., Riles L., St Peter H., Trevaskis E., Vaughan K., Vignati D., Wilcox L., Wohldman P., Waterston R., Wilson R., Vaudin M.
Science 265:2077-2082(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[2]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[3]"Verification of 3' and 5' ends of S.cerevisiae transcripts."
Kennedy M.C., Dietrich F.S.
Submitted (SEP-2003) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-72 AND 478-501.
Strain: ATCC 204511 / S288c / AB972.
[4]"18S rRNA processing requires the RNA helicase-like protein Rrp3."
O'Day C.L., Chavanikamannil F., Abelson J.
Nucleic Acids Res. 24:3201-3207(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[5]"Sequencing and comparison of yeast species to identify genes and regulatory elements."
Kellis M., Patterson N., Endrizzi M., Birren B.W., Lander E.S.
Nature 423:241-254(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION OF PROBABLE INITIATION SITE.
[6]"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-43; SER-45 AND SER-47, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Strain: ADR376.
[7]"A multidimensional chromatography technology for in-depth phosphoproteome analysis."
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[8]"Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-43; SER-45 AND SER-47, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U00061 Genomic DNA. Translation: AAB68392.1. Different initiation.
AY389302 mRNA. Translation: AAQ97234.1.
AY389303 mRNA. Translation: AAQ97235.1.
BK006934 Genomic DNA. Translation: DAA06758.1.
PIRS46713.
RefSeqNP_011932.2. NM_001179195.1.

3D structure databases

ProteinModelPortalP38712.
SMRP38712. Positions 76-457.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid36497. 15 interactions.
DIPDIP-6533N.
IntActP38712. 1 interaction.
MINTMINT-518716.

Proteomic databases

PaxDbP38712.
PeptideAtlasP38712.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYHR065C; YHR065C; YHR065C.
GeneID856462.
KEGGsce:YHR065C.

Organism-specific databases

CYGDYHR065c.
SGDS000001107. RRP3.

Phylogenomic databases

eggNOGCOG0513.
GeneTreeENSGT00730000110914.
HOGENOMHOG000268802.
KOK14777.
OMAKFRAGSR.
OrthoDBEOG7FR7R5.

Enzyme and pathway databases

BioCycYEAST:G3O-31116-MONOMER.

Gene expression databases

GenevestigatorP38712.

Family and domain databases

Gene3D3.40.50.300. 2 hits.
InterProIPR011545. DNA/RNA_helicase_DEAD/DEAH_N.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
IPR000629. RNA-helicase_DEAD-box_CS.
IPR014014. RNA_helicase_DEAD_Q_motif.
[Graphical view]
PfamPF00270. DEAD. 1 hit.
PF00271. Helicase_C. 1 hit.
[Graphical view]
SMARTSM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
[Graphical view]
SUPFAMSSF52540. SSF52540. 1 hit.
PROSITEPS00039. DEAD_ATP_HELICASE. 1 hit.
PS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
PS51195. Q_MOTIF. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio982111.
PROP38712.

Entry information

Entry nameRRP3_YEAST
AccessionPrimary (citable) accession number: P38712
Secondary accession number(s): D3DL14, Q6TQT5, Q6TQT6
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: November 8, 2005
Last modified: April 16, 2014
This is version 124 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome VIII

Yeast (Saccharomyces cerevisiae) chromosome VIII: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families