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P38712

- RRP3_YEAST

UniProt

P38712 - RRP3_YEAST

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Protein

ATP-dependent rRNA helicase RRP3

Gene

RRP3

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Required for pre-ribosomal RNA processing. Involved in the maturation of the 35S-pre-rRNA and to its cleavage to mature 18S rRNA.1 Publication

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi125 – 1328ATPPROSITE-ProRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. ATP-dependent RNA helicase activity Source: SGD
  3. RNA binding Source: UniProtKB-KW
  4. RNA-dependent ATPase activity Source: SGD

GO - Biological processi

  1. ATP catabolic process Source: GOC
  2. maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Helicase, Hydrolase

Keywords - Biological processi

Ribosome biogenesis, rRNA processing

Keywords - Ligandi

ATP-binding, Nucleotide-binding, RNA-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-31116-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent rRNA helicase RRP3 (EC:3.6.4.-)
Alternative name(s):
Ribosomal RNA-processing protein 3
Gene namesi
Name:RRP3
Ordered Locus Names:YHR065C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome VIII

Organism-specific databases

CYGDiYHR065c.
SGDiS000001107. RRP3.

Subcellular locationi

Nucleus Curated

GO - Cellular componenti

  1. nucleolus Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 501501ATP-dependent rRNA helicase RRP3PRO_0000055064Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei43 – 431Phosphoserine2 Publications
Modified residuei45 – 451Phosphoserine2 Publications
Modified residuei47 – 471Phosphoserine2 Publications

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP38712.
PaxDbiP38712.
PeptideAtlasiP38712.

Expressioni

Gene expression databases

GenevestigatoriP38712.

Interactioni

Protein-protein interaction databases

BioGridi36497. 15 interactions.
DIPiDIP-6533N.
IntActiP38712. 1 interaction.
MINTiMINT-518716.

Structurei

3D structure databases

ProteinModelPortaliP38712.
SMRiP38712. Positions 67-446.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini112 – 284173Helicase ATP-bindingPROSITE-ProRule annotationAdd
BLAST
Domaini307 – 461155Helicase C-terminalPROSITE-ProRule annotationAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili3 – 4442Sequence AnalysisAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi81 – 10929Q motifAdd
BLAST
Motifi231 – 2344DEAD box

Domaini

The Q motif is unique to and characteristic of the DEAD box family of RNA helicases and controls ATP binding and hydrolysis.

Sequence similaritiesi

Contains 1 helicase ATP-binding domain.PROSITE-ProRule annotation
Contains 1 helicase C-terminal domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiCOG0513.
GeneTreeiENSGT00730000110914.
HOGENOMiHOG000268802.
InParanoidiP38712.
KOiK14777.
OMAiNKFRAGS.
OrthoDBiEOG7FR7R5.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR011545. DEAD/DEAH_box_helicase_dom.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
IPR000629. RNA-helicase_DEAD-box_CS.
IPR014014. RNA_helicase_DEAD_Q_motif.
[Graphical view]
PfamiPF00270. DEAD. 1 hit.
PF00271. Helicase_C. 1 hit.
[Graphical view]
SMARTiSM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS00039. DEAD_ATP_HELICASE. 1 hit.
PS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
PS51195. Q_MOTIF. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P38712-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSKIVKRKEK KANDELTSLA EKIRAKALEN QKKLIEAEKE GGSESDSEED
60 70 80 90 100
ATAEKKKVLK SKSKSTVSTQ NENTNEDESF ESFSELNLVP ELIQACKNLN
110 120 130 140 150
YSKPTPIQSK AIPPALEGHD IIGLAQTGSG KTAAFAIPIL NRLWHDQEPY
160 170 180 190 200
YACILAPTRE LAQQIKETFD SLGSLMGVRS TCIVGGMNMM DQARDLMRKP
210 220 230 240 250
HIIIATPGRL MDHLENTKGF SLRKLKFLVM DEADRLLDME FGPVLDRILK
260 270 280 290 300
IIPTQERTTY LFSATMTSKI DKLQRASLTN PVKCAVSNKY QTVDTLVQTL
310 320 330 340 350
MVVPGGLKNT YLIYLLNEFI GKTMIIFTRT KANAERLSGL CNLLEFSATA
360 370 380 390 400
LHGDLNQNQR MGSLDLFKAG KRSILVATDV AARGLDIPSV DIVVNYDIPV
410 420 430 440 450
DSKSYIHRVG RTARAGRSGK SISLVSQYDL ELILRIEEVL GKKLPKESVD
460 470 480 490 500
KNIILTLRDS VDKANGEVVM EMNRRNKEKI ARGKGRRGRM MTRENMDMGE

R
Length:501
Mass (Da):55,969
Last modified:November 8, 2005 - v2
Checksum:iAA17798E0897272F
GO

Sequence cautioni

The sequence AAB68392.1 differs from that shown. Reason: Erroneous initiation. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00061 Genomic DNA. Translation: AAB68392.1. Different initiation.
AY389302 mRNA. Translation: AAQ97234.1.
AY389303 mRNA. Translation: AAQ97235.1.
BK006934 Genomic DNA. Translation: DAA06758.1.
PIRiS46713.
RefSeqiNP_011932.2. NM_001179195.1.

Genome annotation databases

EnsemblFungiiYHR065C; YHR065C; YHR065C.
GeneIDi856462.
KEGGisce:YHR065C.

Cross-referencesi

Web resourcesi

RNA helicases from the baker's yeast Saccharomyces cerevisiae

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00061 Genomic DNA. Translation: AAB68392.1 . Different initiation.
AY389302 mRNA. Translation: AAQ97234.1 .
AY389303 mRNA. Translation: AAQ97235.1 .
BK006934 Genomic DNA. Translation: DAA06758.1 .
PIRi S46713.
RefSeqi NP_011932.2. NM_001179195.1.

3D structure databases

ProteinModelPortali P38712.
SMRi P38712. Positions 67-446.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 36497. 15 interactions.
DIPi DIP-6533N.
IntActi P38712. 1 interaction.
MINTi MINT-518716.

Proteomic databases

MaxQBi P38712.
PaxDbi P38712.
PeptideAtlasi P38712.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YHR065C ; YHR065C ; YHR065C .
GeneIDi 856462.
KEGGi sce:YHR065C.

Organism-specific databases

CYGDi YHR065c.
SGDi S000001107. RRP3.

Phylogenomic databases

eggNOGi COG0513.
GeneTreei ENSGT00730000110914.
HOGENOMi HOG000268802.
InParanoidi P38712.
KOi K14777.
OMAi NKFRAGS.
OrthoDBi EOG7FR7R5.

Enzyme and pathway databases

BioCyci YEAST:G3O-31116-MONOMER.

Miscellaneous databases

NextBioi 982111.
PROi P38712.

Gene expression databases

Genevestigatori P38712.

Family and domain databases

Gene3Di 3.40.50.300. 2 hits.
InterProi IPR011545. DEAD/DEAH_box_helicase_dom.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
IPR000629. RNA-helicase_DEAD-box_CS.
IPR014014. RNA_helicase_DEAD_Q_motif.
[Graphical view ]
Pfami PF00270. DEAD. 1 hit.
PF00271. Helicase_C. 1 hit.
[Graphical view ]
SMARTi SM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
[Graphical view ]
SUPFAMi SSF52540. SSF52540. 1 hit.
PROSITEi PS00039. DEAD_ATP_HELICASE. 1 hit.
PS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
PS51195. Q_MOTIF. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. "Verification of 3' and 5' ends of S.cerevisiae transcripts."
    Kennedy M.C., Dietrich F.S.
    Submitted (SEP-2003) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-72 AND 478-501.
    Strain: ATCC 204511 / S288c / AB972.
  4. "18S rRNA processing requires the RNA helicase-like protein Rrp3."
    O'Day C.L., Chavanikamannil F., Abelson J.
    Nucleic Acids Res. 24:3201-3207(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  5. "Sequencing and comparison of yeast species to identify genes and regulatory elements."
    Kellis M., Patterson N., Endrizzi M., Birren B.W., Lander E.S.
    Nature 423:241-254(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION OF PROBABLE INITIATION SITE.
  6. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
    Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
    J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-43; SER-45 AND SER-47, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: ADR376.
  7. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  8. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-43; SER-45 AND SER-47, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiRRP3_YEAST
AccessioniPrimary (citable) accession number: P38712
Secondary accession number(s): D3DL14, Q6TQT5, Q6TQT6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: November 8, 2005
Last modified: October 29, 2014
This is version 128 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome VIII
    Yeast (Saccharomyces cerevisiae) chromosome VIII: entries and gene names

External Data

Dasty 3