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Protein

ATP-dependent rRNA helicase RRP3

Gene

RRP3

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

ATP-dependent rRNA helicase required for pre-ribosomal RNA processing. Involved in the maturation of the 35S-pre-rRNA and to its cleavage to mature 18S rRNA.4 Publications

Catalytic activityi

ATP + H2O = ADP + phosphate.3 Publications

Enzyme regulationi

ATPase activity is stimulated upon the addition of RNA.2 Publications

Kineticsi

  1. KM=2.3 mM for ATP1 Publication

    pH dependencei

    Optimum pH is 7.0-9.0.1 Publication

    Regions

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Nucleotide bindingi125 – 132ATPPROSITE-ProRule annotation8

    GO - Molecular functioni

    • ATP binding Source: UniProtKB-KW
    • ATP-dependent RNA helicase activity Source: SGD
    • RNA binding Source: UniProtKB-KW
    • RNA-dependent ATPase activity Source: SGD

    GO - Biological processi

    Keywordsi

    Molecular functionHelicase, Hydrolase, RNA-binding
    Biological processRibosome biogenesis, rRNA processing
    LigandATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciYEAST:G3O-31116-MONOMER
    ReactomeiR-SCE-6791226 Major pathway of rRNA processing in the nucleolus and cytosol

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    ATP-dependent rRNA helicase RRP3Curated (EC:3.6.4.133 Publications)
    Alternative name(s):
    Ribosomal RNA-processing protein 31 Publication
    Gene namesi
    Name:RRP31 Publication
    Ordered Locus Names:YHR065CImported
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    Proteomesi
    • UP000002311 Componenti: Chromosome VIII

    Organism-specific databases

    EuPathDBiFungiDB:YHR065C
    SGDiS000001107 RRP3

    Subcellular locationi

    Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

    Keywords - Cellular componenti

    Nucleus

    Pathology & Biotechi

    Disruption phenotypei

    Leads to improper processing of 35S precursor rRNA.1 Publication

    Mutagenesis

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Mutagenesisi131K → A or R: Leads to defects in A1 and A2 processing. 1 Publication1
    Mutagenesisi231D → A: Leads to defects in A1 and A2 processing. 1 Publication1
    Mutagenesisi263S → L: Leads to defects in A1 and A2 processing. 1 Publication1

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00000550641 – 501ATP-dependent rRNA helicase RRP3Add BLAST501

    Amino acid modifications

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Modified residuei43PhosphoserineCombined sources1
    Modified residuei45PhosphoserineCombined sources1
    Modified residuei47PhosphoserineCombined sources1

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiP38712
    PaxDbiP38712
    PRIDEiP38712

    PTM databases

    iPTMnetiP38712

    Interactioni

    Subunit structurei

    Interacts with the SSU processome.1 Publication

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    ESF1Q063444EBI-16007,EBI-34121

    Protein-protein interaction databases

    BioGridi36497, 121 interactors
    DIPiDIP-6533N
    IntActiP38712, 8 interactors
    MINTiP38712
    STRINGi4932.YHR065C

    Structurei

    3D structure databases

    ProteinModelPortaliP38712
    SMRiP38712
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Domaini112 – 284Helicase ATP-bindingPROSITE-ProRule annotationAdd BLAST173
    Domaini307 – 461Helicase C-terminalPROSITE-ProRule annotationAdd BLAST155

    Coiled coil

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Coiled coili3 – 44Sequence analysisAdd BLAST42

    Motif

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Motifi81 – 109Q motifCuratedAdd BLAST29
    Motifi231 – 234DEAD boxCurated4

    Domaini

    The Q motif is unique to and characteristic of the DEAD box family of RNA helicases and controls ATP binding and hydrolysis.Curated

    Sequence similaritiesi

    Keywords - Domaini

    Coiled coil

    Phylogenomic databases

    GeneTreeiENSGT00730000110914
    HOGENOMiHOG000268802
    InParanoidiP38712
    KOiK14777
    OMAiLNMDFEV
    OrthoDBiEOG092C1VLP

    Family and domain databases

    CDDicd00079 HELICc, 1 hit
    InterProiView protein in InterPro
    IPR011545 DEAD/DEAH_box_helicase_dom
    IPR014001 Helicase_ATP-bd
    IPR001650 Helicase_C
    IPR027417 P-loop_NTPase
    IPR000629 RNA-helicase_DEAD-box_CS
    IPR014014 RNA_helicase_DEAD_Q_motif
    PfamiView protein in Pfam
    PF00270 DEAD, 1 hit
    PF00271 Helicase_C, 1 hit
    SMARTiView protein in SMART
    SM00487 DEXDc, 1 hit
    SM00490 HELICc, 1 hit
    SUPFAMiSSF52540 SSF52540, 1 hit
    PROSITEiView protein in PROSITE
    PS00039 DEAD_ATP_HELICASE, 1 hit
    PS51192 HELICASE_ATP_BIND_1, 1 hit
    PS51194 HELICASE_CTER, 1 hit
    PS51195 Q_MOTIF, 1 hit

    Sequencei

    Sequence statusi: Complete.

    P38712-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MSKIVKRKEK KANDELTSLA EKIRAKALEN QKKLIEAEKE GGSESDSEED
    60 70 80 90 100
    ATAEKKKVLK SKSKSTVSTQ NENTNEDESF ESFSELNLVP ELIQACKNLN
    110 120 130 140 150
    YSKPTPIQSK AIPPALEGHD IIGLAQTGSG KTAAFAIPIL NRLWHDQEPY
    160 170 180 190 200
    YACILAPTRE LAQQIKETFD SLGSLMGVRS TCIVGGMNMM DQARDLMRKP
    210 220 230 240 250
    HIIIATPGRL MDHLENTKGF SLRKLKFLVM DEADRLLDME FGPVLDRILK
    260 270 280 290 300
    IIPTQERTTY LFSATMTSKI DKLQRASLTN PVKCAVSNKY QTVDTLVQTL
    310 320 330 340 350
    MVVPGGLKNT YLIYLLNEFI GKTMIIFTRT KANAERLSGL CNLLEFSATA
    360 370 380 390 400
    LHGDLNQNQR MGSLDLFKAG KRSILVATDV AARGLDIPSV DIVVNYDIPV
    410 420 430 440 450
    DSKSYIHRVG RTARAGRSGK SISLVSQYDL ELILRIEEVL GKKLPKESVD
    460 470 480 490 500
    KNIILTLRDS VDKANGEVVM EMNRRNKEKI ARGKGRRGRM MTRENMDMGE

    R
    Length:501
    Mass (Da):55,969
    Last modified:November 8, 2005 - v2
    Checksum:iAA17798E0897272F
    GO

    Sequence cautioni

    The sequence AAB68392 differs from that shown. Reason: Erroneous initiation.Curated

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    U00061 Genomic DNA Translation: AAB68392.1 Different initiation.
    AY389302 mRNA Translation: AAQ97234.1
    AY389303 mRNA Translation: AAQ97235.1
    BK006934 Genomic DNA Translation: DAA06758.1
    PIRiS46713
    RefSeqiNP_011932.2, NM_001179195.1

    Genome annotation databases

    EnsemblFungiiYHR065C; YHR065C; YHR065C
    GeneIDi856462
    KEGGisce:YHR065C

    Similar proteinsi

    Entry informationi

    Entry nameiRRP3_YEAST
    AccessioniPrimary (citable) accession number: P38712
    Secondary accession number(s): D3DL14, Q6TQT5, Q6TQT6
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1995
    Last sequence update: November 8, 2005
    Last modified: April 25, 2018
    This is version 157 of the entry and version 2 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome
    UniProt is an ELIXIR core data resource
    Main funding by: National Institutes of Health