Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Inositol monophosphatase 1

Gene

INM1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Responsible for the provision of inositol required for synthesis of phosphatidylinositol and polyphosphoinositides.By similarity2 Publications

Catalytic activityi

Myo-inositol phosphate + H2O = myo-inositol + phosphate.

Cofactori

Enzyme regulationi

Inhibited by Li+ and Na+.1 Publication

Kineticsi

  1. KM=0.08 mM for inositol 1-phosphate1 Publication
  1. Vmax=20 µmol/min/mg enzyme for inositol 1-phosphate1 Publication

Pathwayi: myo-inositol biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes myo-inositol from D-glucose 6-phosphate.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Inositol-3-phosphate synthase (INO1)
  2. Inositol monophosphatase 2 (INM2), Inositol monophosphatase 1 (INM1)
This subpathway is part of the pathway myo-inositol biosynthesis, which is itself part of Polyol metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes myo-inositol from D-glucose 6-phosphate, the pathway myo-inositol biosynthesis and in Polyol metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi73 – 731Magnesium 1By similarity
Binding sitei73 – 731SubstrateBy similarity
Metal bindingi92 – 921Magnesium 1By similarity
Metal bindingi92 – 921Magnesium 2By similarity
Metal bindingi94 – 941Magnesium 1; via carbonyl oxygenBy similarity
Metal bindingi95 – 951Magnesium 2By similarity
Metal bindingi231 – 2311Magnesium 2By similarity
Binding sitei231 – 2311SubstrateBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

Lithium, Magnesium, Metal-binding

Enzyme and pathway databases

BioCyciYEAST:YHR046C-MONOMER.
ReactomeiR-SCE-1855183. Synthesis of IP2, IP, and Ins in the cytosol.
UniPathwayiUPA00823; UER00788.

Names & Taxonomyi

Protein namesi
Recommended name:
Inositol monophosphatase 1 (EC:3.1.3.25)
Short name:
IMP 1
Short name:
IMPase 1
Alternative name(s):
Inositol-1(or 4)-monophosphatase 1
Gene namesi
Name:INM1
Synonyms:IMP1
Ordered Locus Names:YHR046C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VIII

Organism-specific databases

EuPathDBiFungiDB:YHR046C.
SGDiS000001088. INM1.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 295295Inositol monophosphatase 1PRO_0000142585Add
BLAST

Proteomic databases

MaxQBiP38710.
PeptideAtlasiP38710.

Interactioni

Protein-protein interaction databases

BioGridi36478. 31 interactions.
DIPiDIP-6506N.
MINTiMINT-2734649.

Structurei

3D structure databases

ProteinModelPortaliP38710.
SMRiP38710. Positions 5-272.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni94 – 974Substrate bindingBy similarity

Sequence similaritiesi

Belongs to the inositol monophosphatase family.Curated

Phylogenomic databases

GeneTreeiENSGT00390000014699.
HOGENOMiHOG000282238.
InParanoidiP38710.
KOiK01092.
OMAiYINDATH.
OrthoDBiEOG7RBZKG.

Family and domain databases

InterProiIPR020583. Inositol_monoP_metal-BS.
IPR000760. Inositol_monophosphatase.
IPR020550. Inositol_monophosphatase_CS.
[Graphical view]
PANTHERiPTHR20854. PTHR20854. 1 hit.
PfamiPF00459. Inositol_P. 1 hit.
[Graphical view]
PRINTSiPR00377. IMPHPHTASES.
PROSITEiPS00629. IMP_1. 1 hit.
PS00630. IMP_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P38710-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTIDLASIEK FLCELATEKV GPIIKSKSGT QKDYDLKTGS RSVDIVTAID
60 70 80 90 100
KQVEKLIWES VKTQYPTFKF IGEESYVKGE TVITDDPTFI IDPIDGTTNF
110 120 130 140 150
VHDFPFSCTS LGLTVNKEPV VGVIYNPHIN LLVSASKGNG MRVNNKDYDY
160 170 180 190 200
KSKLESMGSL ILNKSVVALQ PGSAREGKNF QTKMATYEKL LSCDYGFVHG
210 220 230 240 250
FRNLGSSAMT MAYIAMGYLD SYWDGGCYSW DVCAGWCILK EVGGRVVGAN
260 270 280 290
PGEWSIDVDN RTYLAVRGTI NNESDEQTKY ITDFWNCVDG HLKYD
Length:295
Mass (Da):32,823
Last modified:February 1, 1995 - v1
Checksum:i1DF052114912C6CB
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00062 Genomic DNA. Translation: AAB68918.1.
BK006934 Genomic DNA. Translation: DAA06738.1.
PIRiS46749.
RefSeqiNP_011912.1. NM_001179176.1.

Genome annotation databases

EnsemblFungiiYHR046C; YHR046C; YHR046C.
GeneIDi856442.
KEGGisce:YHR046C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00062 Genomic DNA. Translation: AAB68918.1.
BK006934 Genomic DNA. Translation: DAA06738.1.
PIRiS46749.
RefSeqiNP_011912.1. NM_001179176.1.

3D structure databases

ProteinModelPortaliP38710.
SMRiP38710. Positions 5-272.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi36478. 31 interactions.
DIPiDIP-6506N.
MINTiMINT-2734649.

Proteomic databases

MaxQBiP38710.
PeptideAtlasiP38710.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYHR046C; YHR046C; YHR046C.
GeneIDi856442.
KEGGisce:YHR046C.

Organism-specific databases

EuPathDBiFungiDB:YHR046C.
SGDiS000001088. INM1.

Phylogenomic databases

GeneTreeiENSGT00390000014699.
HOGENOMiHOG000282238.
InParanoidiP38710.
KOiK01092.
OMAiYINDATH.
OrthoDBiEOG7RBZKG.

Enzyme and pathway databases

UniPathwayiUPA00823; UER00788.
BioCyciYEAST:YHR046C-MONOMER.
ReactomeiR-SCE-1855183. Synthesis of IP2, IP, and Ins in the cytosol.

Miscellaneous databases

PROiP38710.

Family and domain databases

InterProiIPR020583. Inositol_monoP_metal-BS.
IPR000760. Inositol_monophosphatase.
IPR020550. Inositol_monophosphatase_CS.
[Graphical view]
PANTHERiPTHR20854. PTHR20854. 1 hit.
PfamiPF00459. Inositol_P. 1 hit.
[Graphical view]
PRINTSiPR00377. IMPHPHTASES.
PROSITEiPS00629. IMP_1. 1 hit.
PS00630. IMP_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. "The yeast inositol monophosphatase is a lithium- and sodium-sensitive enzyme encoded by a non-essential gene pair."
    Lopez F., Leube M., Gil-Mascarell R., Navarro-Avino J.P., Serrano R.
    Mol. Microbiol. 31:1255-1264(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, ENZYME REGULATION, BIOPHYSICOCHEMICAL PROPERTIES.
  4. "Yeast inositol mono- and trisphosphate levels are modulated by inositol monophosphatase activity and nutrients."
    Navarro-Avino J.P., Belles J.M., Serrano R.
    Biochem. Biophys. Res. Commun. 302:41-45(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  5. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiINM1_YEAST
AccessioniPrimary (citable) accession number: P38710
Secondary accession number(s): D3DKZ4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: June 8, 2016
This is version 130 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 2440 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome VIII
    Yeast (Saccharomyces cerevisiae) chromosome VIII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.