P38710 (INM1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Inositol monophosphatase 1
Short name=IMP 1
Short name=IMPase 1
EC=3.1.3.25

Alternative name(s):
Inositol-1(or 4)-monophosphatase 1

Gene names

Name:	INM1
Synonyms:	IMP1
Ordered Locus Names:	YHR046C

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	295 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Responsible for the provision of inositol required for synthesis of phosphatidylinositol and polyphosphoinositides By similarity. Ref.3 Ref.4
Catalytic activity	Myo-inositol phosphate + H2O = myo-inositol + phosphate.
Cofactor	Magnesium.
Enzyme regulation	Inhibited by Li+ and Na+. Ref.3
Pathway	Polyol metabolism; myo-inositol biosynthesis; myo-inositol from D-glucose 6-phosphate: step 2/2.
Subcellular location	Cytoplasm. Nucleus Ref.5.
Miscellaneous	Present with 2440 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the inositol monophosphatase family.
Biophysicochemical properties	Kinetic parameters: KM=0.08 mM for inositol 1-phosphate Ref.3 Vmax=20 µmol/min/mg enzyme for inositol 1-phosphate

Ontologies

Keywords
Cellular component	Cytoplasm Nucleus
Ligand	Lithium Magnesium Metal-binding
Molecular function	Hydrolase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	inositol biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway inositol phosphate dephosphorylation Inferred from direct assay PubMed 10844654. Source: SGD phosphatidylinositol phosphorylation Inferred from electronic annotation. Source: InterPro
Cellular_component	cytoplasm Inferred from direct assay Ref.5. Source: SGD nucleus Inferred from direct assay Ref.5. Source: SGD
Molecular_function	inositol monophosphate 1-phosphatase activity Inferred from direct assay Ref.3. Source: SGD inositol monophosphate 3-phosphatase activity Inferred from electronic annotation. Source: EC inositol monophosphate 4-phosphatase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 295	295	Inositol monophosphatase 1		PRO_0000142585
Regions
	Region	94 – 97	4	Substrate binding By similarity
Sites
	Metal binding	73	1	Magnesium 1 By similarity
	Metal binding	92	1	Magnesium 1 By similarity
	Metal binding	92	1	Magnesium 2 By similarity
	Metal binding	94	1	Magnesium 1; via carbonyl oxygen By similarity
	Metal binding	95	1	Magnesium 2 By similarity
	Metal binding	231	1	Magnesium 2 By similarity
	Binding site	73	1	Substrate By similarity
	Binding site	231	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	P38710 [UniParc]. Last modified February 1, 1995. Version 1. Checksum: 1DF052114912C6CB	FASTA	295	32,823
	10 20 30 40 50 60 MTIDLASIEK FLCELATEKV GPIIKSKSGT QKDYDLKTGS RSVDIVTAID KQVEKLIWES 70 80 90 100 110 120 VKTQYPTFKF IGEESYVKGE TVITDDPTFI IDPIDGTTNF VHDFPFSCTS LGLTVNKEPV 130 140 150 160 170 180 VGVIYNPHIN LLVSASKGNG MRVNNKDYDY KSKLESMGSL ILNKSVVALQ PGSAREGKNF 190 200 210 220 230 240 QTKMATYEKL LSCDYGFVHG FRNLGSSAMT MAYIAMGYLD SYWDGGCYSW DVCAGWCILK 250 260 270 280 290 EVGGRVVGAN PGEWSIDVDN RTYLAVRGTI NNESDEQTKY ITDFWNCVDG HLKYD

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete nucleotide sequence of Saccharomyces cerevisiae chromosome VIII." Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Dover J., Du Z., Favello A., Fulton L., Gattung S., Geisel C., Kirsten J., Kucaba T., Hillier L.W., Jier M., Johnston L., Langston Y., Latreille P. , Louis E.J., Macri C., Mardis E., Menezes S., Mouser L., Nhan M., Rifkin L., Riles L., St Peter H., Trevaskis E., Vaughan K., Vignati D., Wilcox L., Wohldman P., Waterston R., Wilson R., Vaudin M. Science 265:2077-2082(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[3]	"The yeast inositol monophosphatase is a lithium- and sodium-sensitive enzyme encoded by a non-essential gene pair." Lopez F., Leube M., Gil-Mascarell R., Navarro-Avino J.P., Serrano R. Mol. Microbiol. 31:1255-1264(1999) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, ENZYME REGULATION, BIOPHYSICOCHEMICAL PROPERTIES.
[4]	"Yeast inositol mono- and trisphosphate levels are modulated by inositol monophosphatase activity and nutrients." Navarro-Avino J.P., Belles J.M., Serrano R. Biochem. Biophys. Res. Commun. 302:41-45(2003) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[5]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[6]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U00062 Genomic DNA. Translation: AAB68918.1. BK006934 Genomic DNA. Translation: DAA06738.1.
PIR	S46749.
RefSeq	NP_011912.1. NM_001179176.1.
3D structure databases
ProteinModelPortal	P38710.
SMR	P38710. Positions 4-285.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-6506N.
IntAct	P38710. 1 interaction.
MINT	MINT-2734649.
STRING	4932.YHR046C.
Proteomic databases
PaxDb	P38710.
PeptideAtlas	P38710.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YHR046C; YHR046C; YHR046C.
GeneID	856442.
KEGG	sce:YHR046C.
Organism-specific databases
CYGD	YHR046c.
SGD	S000001088. INM1.
Phylogenomic databases
eggNOG	COG0483.
GeneTree	ENSGT00390000014699.
HOGENOM	HOG000282238.
KO	K01092.
OMA	GYYEPHM.
OrthoDB	EOG40312B.
Enzyme and pathway databases
UniPathway	UPA00823; UER00788.
Gene expression databases
Genevestigator	P38710.
GermOnline	YHR046C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR020583. Inositol_monoP_metal-BS. IPR000760. Inositol_monophosphatase. IPR020550. Inositol_monophosphatase_CS. [Graphical view]
PANTHER	PTHR20854. PTHR20854. 1 hit.
Pfam	PF00459. Inositol_P. 1 hit. [Graphical view]
PRINTS	PR00377. IMPHPHTASES.
PROSITE	PS00629. IMP_1. 1 hit. PS00630. IMP_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	982054.

Entry information

Entry name

INM1_YEAST

Accession

Primary (citable) accession number: P38710
Secondary accession number(s): D3DKZ4

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	February 1, 1995
Last modified:	May 1, 2013
This is version 101 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program