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Protein

Putative proline--tRNA ligase YHR020W

Gene

YHR020W

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + L-proline + tRNA(Pro) = AMP + diphosphate + L-prolyl-tRNA(Pro).

GO - Molecular functioni

  • aminoacyl-tRNA editing activity Source: InterPro
  • ATP binding Source: UniProtKB-KW
  • proline-tRNA ligase activity Source: SGD

GO - Biological processi

  • prolyl-tRNA aminoacylation Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-31081-MONOMER.
SABIO-RKP38708.

Names & Taxonomyi

Protein namesi
Recommended name:
Putative proline--tRNA ligase YHR020W (EC:6.1.1.15)
Alternative name(s):
Prolyl-tRNA synthetase
Short name:
ProRS
Gene namesi
Ordered Locus Names:YHR020W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VIII

Organism-specific databases

EuPathDBiFungiDB:YHR020W.
SGDiS000001062. YHR020W.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 688688Putative proline--tRNA ligase YHR020WPRO_0000139355Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei149 – 1491PhosphoserineCombined sources
Modified residuei170 – 1701PhosphothreonineCombined sources
Modified residuei655 – 6551PhosphoserineCombined sources

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP38708.
PeptideAtlasiP38708.
PRIDEiP38708.

PTM databases

iPTMnetiP38708.

Interactioni

Protein-protein interaction databases

BioGridi36449. 80 interactions.
DIPiDIP-6590N.
IntActiP38708. 56 interactions.
MINTiMINT-664834.

Structurei

3D structure databases

ProteinModelPortaliP38708.
SMRiP38708. Positions 191-688.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

GeneTreeiENSGT00550000074815.
HOGENOMiHOG000167538.
InParanoidiP38708.
KOiK01881.
OMAiIPHCLVP.
OrthoDBiEOG7MWH5Z.

Family and domain databases

Gene3Di3.30.110.30. 1 hit.
3.40.50.800. 1 hit.
3.90.960.10. 1 hit.
HAMAPiMF_01571. Pro_tRNA_synth_type3.
InterProiIPR002314. aa-tRNA-synt_IIb.
IPR006195. aa-tRNA-synth_II.
IPR004154. Anticodon-bd.
IPR002316. Pro-tRNA-ligase_IIa.
IPR004499. Pro-tRNA-ligase_IIa_arc-type.
IPR016061. Pro-tRNA_ligase_II_C.
IPR017449. Pro-tRNA_synth_II.
IPR007214. YbaK/aa-tRNA-synth-assoc-dom.
[Graphical view]
PfamiPF03129. HGTP_anticodon. 1 hit.
PF09180. ProRS-C_1. 1 hit.
PF00587. tRNA-synt_2b. 1 hit.
[Graphical view]
PRINTSiPR01046. TRNASYNTHPRO.
SMARTiSM00946. ProRS-C_1. 1 hit.
[Graphical view]
SUPFAMiSSF52954. SSF52954. 1 hit.
SSF55826. SSF55826. 1 hit.
SSF64586. SSF64586. 1 hit.
TIGRFAMsiTIGR00408. proS_fam_I. 1 hit.
PROSITEiPS50862. AA_TRNA_LIGASE_II. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P38708-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPVSEAFAKL CVNEKPPAES AVAVKSLVFK PKTPKSATPV PIVVVALQST
60 70 80 90 100
TTPSALIANA TSSKDPRLAR DDLVKQAFQS ESARAFILGD LANATSNFHL
110 120 130 140 150
LIDHELGTVD GDTILQLNDS TYMKKSDMMK FLNNFEDSQK VVDFSQEVSK
160 170 180 190 200
ETATEGKKQQ KKQQPSKAGT AAAAAAAALE DAKLIGITVD KALDFPGWYQ
210 220 230 240 250
QILTKGEMLD YYDVSGCYIL RPPSYAIWEN IQKWFDDKIK AIGVQNAYFP
260 270 280 290 300
MFVSSRVLEK EKDHVEGFAP EVAWVTRAGS SELEEPIAIR PTSETVMYPY
310 320 330 340 350
YAKWVQSYRD LPLKLNQWNS VVRWEFKHPQ PFLRTREFLW QEGHTAHLTA
360 370 380 390 400
KDAEEEVLQI LDFYAGVYEE LLAVPVVKGR KTEKEKFAGG DFTTTCEGYI
410 420 430 440 450
PQTGRGIQGA TSHHLGQNFS KMFNLSVENP LGSDHPKIFA YQNSWGLSTR
460 470 480 490 500
VIGVMVMIHS DNKGLVIPPR VSQFQSVVIP VGITKKTSEE QRKHIHETAR
510 520 530 540 550
SVESRLKKVG IRAFGDYNDN YTPGWKFSQY ELKGIPIRIE LGPKDIEKNQ
560 570 580 590 600
VVVVRRNDSK KYVVSFDELE ARIPEILEEM QGDLFKKAKE LFDTHRVIVN
610 620 630 640 650
EWSGFVPALN KKNVILAPWC GVMECEEDIK ESSAKKDDGE EFEEDDKAPS
660 670 680
MGAKSLCIPF DQPVLNEGQK CIKCERIAVN YCMFGRSY
Length:688
Mass (Da):77,386
Last modified:February 1, 1995 - v1
Checksum:i79EB480B24CA8CFC
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U10399 Genomic DNA. Translation: AAB68873.1.
BK006934 Genomic DNA. Translation: DAA06709.1.
PIRiS46774.
RefSeqiNP_011884.1. NM_001179150.1.

Genome annotation databases

EnsemblFungiiYHR020W; YHR020W; YHR020W.
GeneIDi856413.
KEGGisce:YHR020W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U10399 Genomic DNA. Translation: AAB68873.1.
BK006934 Genomic DNA. Translation: DAA06709.1.
PIRiS46774.
RefSeqiNP_011884.1. NM_001179150.1.

3D structure databases

ProteinModelPortaliP38708.
SMRiP38708. Positions 191-688.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi36449. 80 interactions.
DIPiDIP-6590N.
IntActiP38708. 56 interactions.
MINTiMINT-664834.

PTM databases

iPTMnetiP38708.

Proteomic databases

MaxQBiP38708.
PeptideAtlasiP38708.
PRIDEiP38708.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYHR020W; YHR020W; YHR020W.
GeneIDi856413.
KEGGisce:YHR020W.

Organism-specific databases

EuPathDBiFungiDB:YHR020W.
SGDiS000001062. YHR020W.

Phylogenomic databases

GeneTreeiENSGT00550000074815.
HOGENOMiHOG000167538.
InParanoidiP38708.
KOiK01881.
OMAiIPHCLVP.
OrthoDBiEOG7MWH5Z.

Enzyme and pathway databases

BioCyciYEAST:G3O-31081-MONOMER.
SABIO-RKP38708.

Miscellaneous databases

NextBioi981968.
PROiP38708.

Family and domain databases

Gene3Di3.30.110.30. 1 hit.
3.40.50.800. 1 hit.
3.90.960.10. 1 hit.
HAMAPiMF_01571. Pro_tRNA_synth_type3.
InterProiIPR002314. aa-tRNA-synt_IIb.
IPR006195. aa-tRNA-synth_II.
IPR004154. Anticodon-bd.
IPR002316. Pro-tRNA-ligase_IIa.
IPR004499. Pro-tRNA-ligase_IIa_arc-type.
IPR016061. Pro-tRNA_ligase_II_C.
IPR017449. Pro-tRNA_synth_II.
IPR007214. YbaK/aa-tRNA-synth-assoc-dom.
[Graphical view]
PfamiPF03129. HGTP_anticodon. 1 hit.
PF09180. ProRS-C_1. 1 hit.
PF00587. tRNA-synt_2b. 1 hit.
[Graphical view]
PRINTSiPR01046. TRNASYNTHPRO.
SMARTiSM00946. ProRS-C_1. 1 hit.
[Graphical view]
SUPFAMiSSF52954. SSF52954. 1 hit.
SSF55826. SSF55826. 1 hit.
SSF64586. SSF64586. 1 hit.
TIGRFAMsiTIGR00408. proS_fam_I. 1 hit.
PROSITEiPS50862. AA_TRNA_LIGASE_II. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-149 AND THR-170, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  4. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-655, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiYHI0_YEAST
AccessioniPrimary (citable) accession number: P38708
Secondary accession number(s): D3DKW5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: May 11, 2016
This is version 136 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome VIII
    Yeast (Saccharomyces cerevisiae) chromosome VIII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.