Reviewed, UniProtKB/Swiss-Prot P38707 (SYNC_YEAST)

Names and origin

Protein names

Recommended name:
    Asparaginyl-tRNA synthetase, cytoplasmic
    EC=6.1.1.22
Alternative name(s):
    Asparagine--tRNA ligase
      Short name=AsnRS

Gene names

Name:	DED81
Ordered Locus Names:	YHR019C

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	554 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	ATP + L-asparagine + tRNA(Asn) = AMP + diphosphate + L-asparaginyl-tRNA(Asn).
Subcellular location	Cytoplasm.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	asparaginyl-tRNA aminoacylation Ref.2 Inferred from direct assay. Source: SGD aspartyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Ref.2 Traceable author statement. Source: SGD
Molecular function	ATP binding Ref.2 Traceable author statement. Source: SGD asparagine-tRNA ligase activity Ref.2 Inferred from direct assay. Source: SGD aspartate-tRNA ligase activity Inferred from electronic annotation. Source: InterPro nucleic acid binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 554	554	Asparaginyl-tRNA synthetase, cytoplasmic		PRO_0000176501
Amino acid modifications
	Modified residue	101	1	Phosphoserine Ref.3
	Modified residue	112	1	Phosphoserine Ref.3
	Modified residue	229	1	Phosphoserine Ref.3

Sequences

	Sequence		Length	Mass (Da)
	P38707-1 [UniParc]. Last modified February 1, 1995. Version 1. Checksum: 169B6859F694D29C	FASTA	554	62,207
	10 20 30 40 50 60 MSSLYIKEAT GVDELTTAGS QDHPFKTPAY ALFASQQKSD ATEPKLFVFK TEDNEYQEIS 70 80 90 100 110 120 ASALKKARKG CDGLKKKAVK QKEQELKKQQ KEAENAAKQL SALNITIKED ESLPAAIKTR 130 140 150 160 170 180 IYDSYSKVGQ RVKVSGWIHR LRSNKKVIFV VLRDGSGFIQ CVLSGDLALA QQTLDLTLES 190 200 210 220 230 240 TVTLYGTIVK LPEGKTAPGG VELNVDYYEV VGLAPGGEDS FTNKIAEGSD PSLLLDQRHL 250 260 270 280 290 300 ALRGDALSAV MKVRAALLKS VRRVYDEEHL TEVTPPCMVQ TQVEGGSTLF KMNYYGEEAY 310 320 330 340 350 360 LTQSSQLYLE TCLASLGDVY TIQESFRAEK SHTRRHLSEY THIEAELAFL TFDDLLQHIE 370 380 390 400 410 420 TLIVKSVQYV LEDPIAGPLV KQLNPNFKAP KAPFMRLQYK DAITWLNEHD IKNEEGEDFK 430 440 450 460 470 480 FGDDIAEAAE RKMTDTIGVP IFLTRFPVEI KSFYMKRCSD DPRVTESVDV LMPNVGEITG 490 500 510 520 530 540 GSMRIDDMDE LMAGFKREGI DTDAYYWFID QRKYGTCPHG GYGIGTERIL AWLCDRFTVR 550 DCSLYPRFSG RCKP

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete nucleotide sequence of Saccharomyces cerevisiae chromosome VIII." Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Dover J., Du Z., Favello A., Fulton L., Gattung S., Geisel C., Kirsten J., Kucaba T., Hillier L.W., Jier M., Johnston L., Langston Y., Latreille P. , Louis E.J., Macri C., Mardis E., Menezes S., Mouser L., Nhan M., Rifkin L., Riles L., St Peter H., Trevaskis E., Vaughan K., Vignati D., Wilcox L., Wohldman P., Waterston R., Wilson R., Vaudin M. Science 265:2077-2082(1994) [PubMed: 8091229] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	"Identification of YHR019 in Saccharomyces cerevisiae chromosome VIII as the gene for the cytosolic asparaginyl-tRNA synthetase." Landrieu I., Vandenbol M., Leberman R., Portetelle D., Hartlein M. Yeast 14:527-533(1998) [PubMed: 9605503] [Abstract] Cited for: CHARACTERIZATION.
[3]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-101; SER-112 AND SER-229, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U10399 Genomic DNA. Translation: AAB68874.1.
PIR	S46775.
RefSeq	NP_011883.1.
3D structure databases
HSSP	HSSP built from PDB template 1N9W based on UniProtKB Q9LCY8.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:5575N.
IntAct	P38707. 62 interactions.
STRING	P38707.
Proteomic databases
PeptideAtlas	P38707.
PRIDE	P38707.
Genome annotation databases
Ensembl	YHR019C; YHR019C; YHR019C; Saccharomyces cerevisiae. [Genome view]
GeneID	856412.
GenomeReviews	Gene locus YHR019C in contig U00093_GR.
KEGG	sce:YHR019C.
NMPDR	fig|4932.3.peg.3028.
Organism-specific databases
CYGD	YHR019c.
SGD	S000001061. DED81.
Phylogenomic databases
HOGENOM	P38707.
OMA	SEYTHIE.
Enzyme and pathway databases
BRENDA	6.1.1.22. 250.
Gene expression databases
ArrayExpress	P38707.
Genevestigator	P38707.
GermOnline	YHR019C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR004364. aa-tRNA-synt_II. IPR018150. aa-tRNA-synt_II-like. IPR006195. aa-tRNA-synth_II_cons-reg. IPR004522. Asn-tRNA-synth_IIb. IPR002312. Asp-tRNA-synth_IIb. IPR012340. NA-bd_OB-fold. IPR004365. NA_bd_OB_tRNA-helicase. [Graphical view]
Gene3D	G3DSA:2.40.50.140. OB_NA_bd_sub. 1 hit.
PANTHER	PTHR22594. aa-tRNA-synt_II. 1 hit.
Pfam	PF00152. tRNA-synt_2. 1 hit. PF01336. tRNA_anti. 1 hit. [Graphical view]
PRINTS	PR01042. TRNASYNTHASP.
TIGRFAMs	TIGR00457. asnS. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	981965.

Entry information

Entry name

SYNC_YEAST

Accession

Primary (citable) accession number: P38707

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	February 1, 1995
Last modified:	November 3, 2009
This is version 80 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)