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Protein

Asparagine--tRNA ligase, cytoplasmic

Gene

DED81

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + L-asparagine + tRNA(Asn) = AMP + diphosphate + L-asparaginyl-tRNA(Asn).

GO - Molecular functioni

  • asparagine-tRNA ligase activity Source: SGD
  • ATP binding Source: UniProtKB-KW
  • nucleic acid binding Source: InterPro

GO - Biological processi

  • asparaginyl-tRNA aminoacylation Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-31080-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Asparagine--tRNA ligase, cytoplasmic (EC:6.1.1.22)
Alternative name(s):
Asparaginyl-tRNA synthetase
Short name:
AsnRS
Gene namesi
Name:DED81
Ordered Locus Names:YHR019C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VIII

Organism-specific databases

EuPathDBiFungiDB:YHR019C.
SGDiS000001061. DED81.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 554554Asparagine--tRNA ligase, cytoplasmicPRO_0000176501Add
BLAST

Proteomic databases

MaxQBiP38707.
PRIDEiP38707.

PTM databases

iPTMnetiP38707.

Interactioni

Protein-protein interaction databases

BioGridi36448. 184 interactions.
DIPiDIP-5575N.
IntActiP38707. 58 interactions.
MINTiMINT-527122.

Structurei

3D structure databases

ProteinModelPortaliP38707.
SMRiP38707. Positions 93-554.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

GeneTreeiENSGT00550000074893.
HOGENOMiHOG000226035.
InParanoidiP38707.
KOiK01893.
OMAiWGDDIGA.
OrthoDBiEOG092C1DI9.

Family and domain databases

Gene3Di2.40.50.140. 1 hit.
InterProiIPR004364. aa-tRNA-synt_II.
IPR018150. aa-tRNA-synt_II-like.
IPR006195. aa-tRNA-synth_II.
IPR004522. Asn-tRNA-ligase.
IPR002312. Asp/Asn-tRNA-synth_IIb.
IPR012340. NA-bd_OB-fold.
IPR004365. NA-bd_OB_tRNA.
[Graphical view]
PANTHERiPTHR22594. PTHR22594. 1 hit.
PTHR22594:SF16. PTHR22594:SF16. 1 hit.
PfamiPF00152. tRNA-synt_2. 1 hit.
PF01336. tRNA_anti-codon. 1 hit.
[Graphical view]
PRINTSiPR01042. TRNASYNTHASP.
SUPFAMiSSF50249. SSF50249. 1 hit.
TIGRFAMsiTIGR00457. asnS. 1 hit.
PROSITEiPS50862. AA_TRNA_LIGASE_II. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P38707-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSLYIKEAT GVDELTTAGS QDHPFKTPAY ALFASQQKSD ATEPKLFVFK
60 70 80 90 100
TEDNEYQEIS ASALKKARKG CDGLKKKAVK QKEQELKKQQ KEAENAAKQL
110 120 130 140 150
SALNITIKED ESLPAAIKTR IYDSYSKVGQ RVKVSGWIHR LRSNKKVIFV
160 170 180 190 200
VLRDGSGFIQ CVLSGDLALA QQTLDLTLES TVTLYGTIVK LPEGKTAPGG
210 220 230 240 250
VELNVDYYEV VGLAPGGEDS FTNKIAEGSD PSLLLDQRHL ALRGDALSAV
260 270 280 290 300
MKVRAALLKS VRRVYDEEHL TEVTPPCMVQ TQVEGGSTLF KMNYYGEEAY
310 320 330 340 350
LTQSSQLYLE TCLASLGDVY TIQESFRAEK SHTRRHLSEY THIEAELAFL
360 370 380 390 400
TFDDLLQHIE TLIVKSVQYV LEDPIAGPLV KQLNPNFKAP KAPFMRLQYK
410 420 430 440 450
DAITWLNEHD IKNEEGEDFK FGDDIAEAAE RKMTDTIGVP IFLTRFPVEI
460 470 480 490 500
KSFYMKRCSD DPRVTESVDV LMPNVGEITG GSMRIDDMDE LMAGFKREGI
510 520 530 540 550
DTDAYYWFID QRKYGTCPHG GYGIGTERIL AWLCDRFTVR DCSLYPRFSG

RCKP
Length:554
Mass (Da):62,207
Last modified:February 1, 1995 - v1
Checksum:i169B6859F694D29C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U10399 Genomic DNA. Translation: AAB68874.1.
BK006934 Genomic DNA. Translation: DAA06708.1.
PIRiS46775.
RefSeqiNP_011883.1. NM_001179149.1.

Genome annotation databases

EnsemblFungiiYHR019C; YHR019C; YHR019C.
GeneIDi856412.
KEGGisce:YHR019C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U10399 Genomic DNA. Translation: AAB68874.1.
BK006934 Genomic DNA. Translation: DAA06708.1.
PIRiS46775.
RefSeqiNP_011883.1. NM_001179149.1.

3D structure databases

ProteinModelPortaliP38707.
SMRiP38707. Positions 93-554.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi36448. 184 interactions.
DIPiDIP-5575N.
IntActiP38707. 58 interactions.
MINTiMINT-527122.

PTM databases

iPTMnetiP38707.

Proteomic databases

MaxQBiP38707.
PRIDEiP38707.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYHR019C; YHR019C; YHR019C.
GeneIDi856412.
KEGGisce:YHR019C.

Organism-specific databases

EuPathDBiFungiDB:YHR019C.
SGDiS000001061. DED81.

Phylogenomic databases

GeneTreeiENSGT00550000074893.
HOGENOMiHOG000226035.
InParanoidiP38707.
KOiK01893.
OMAiWGDDIGA.
OrthoDBiEOG092C1DI9.

Enzyme and pathway databases

BioCyciYEAST:G3O-31080-MONOMER.

Miscellaneous databases

PROiP38707.

Family and domain databases

Gene3Di2.40.50.140. 1 hit.
InterProiIPR004364. aa-tRNA-synt_II.
IPR018150. aa-tRNA-synt_II-like.
IPR006195. aa-tRNA-synth_II.
IPR004522. Asn-tRNA-ligase.
IPR002312. Asp/Asn-tRNA-synth_IIb.
IPR012340. NA-bd_OB-fold.
IPR004365. NA-bd_OB_tRNA.
[Graphical view]
PANTHERiPTHR22594. PTHR22594. 1 hit.
PTHR22594:SF16. PTHR22594:SF16. 1 hit.
PfamiPF00152. tRNA-synt_2. 1 hit.
PF01336. tRNA_anti-codon. 1 hit.
[Graphical view]
PRINTSiPR01042. TRNASYNTHASP.
SUPFAMiSSF50249. SSF50249. 1 hit.
TIGRFAMsiTIGR00457. asnS. 1 hit.
PROSITEiPS50862. AA_TRNA_LIGASE_II. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSYNC_YEAST
AccessioniPrimary (citable) accession number: P38707
Secondary accession number(s): D3DKW4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: September 7, 2016
This is version 140 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome VIII
    Yeast (Saccharomyces cerevisiae) chromosome VIII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.