Reviewed, UniProtKB/Swiss-Prot P38705 (SYSM_YEAST)

Names and origin

Protein names

Recommended name:
    Seryl-tRNA synthetase, mitochondrial
    EC=6.1.1.11
Alternative name(s):
    Seryl-tRNA(Ser/Sec) synthetase
    Serine--tRNA ligase
      Short name=SerRS
    Digs into agar protein 4

Gene names

Name:	DIA4
Ordered Locus Names:	YHR011W

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	446 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Catalyzes the attachment of serine to tRNA(Ser). Is also able to aminoacylate tRNA(Sec) with serine, to form the misacylated tRNA L-seryl-tRNA(Sec), which will be further converted into selenocysteinyl-tRNA(Sec) By similarity.
Catalytic activity	ATP + L-serine + tRNA(Ser) = AMP + diphosphate + L-seryl-tRNA(Ser). ATP + L-serine + tRNA(Sec) = AMP + diphosphate + L-seryl-tRNA(Sec).
Pathway	Aminoacyl-tRNA biosynthesis; selenocysteinyl-tRNA(Sec) biosynthesis; L-seryl-tRNA(Sec) from L-serine and tRNA(Sec): step 1/1.
Subunit structure	Homodimer. The tRNA molecule binds across the dimer By similarity.
Subcellular location	Mitochondrion matrix. Ref.3
Domain	Consists of two distinct domains, a catalytic core and a N-terminal extension that is involved in tRNA binding By similarity.
Miscellaneous	Present with 405 molecules/cell in log phase SD medium. Ref.4
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family. Type-1 seryl-tRNA synthetase subfamily.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Mitochondrion
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	aerobic respiration Inferred from expression pattern. Source: SGD invasive growth in response to glucose limitation Inferred from genetic interaction. Source: SGD mitochondrial seryl-tRNA aminoacylation Inferred from genetic interaction. Source: SGD pseudohyphal growth Inferred from mutant phenotype. Source: SGD
Cellular component	mitochondrial matrix Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW serine-tRNA ligase activity Inferred from genetic interaction. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 446	446	Seryl-tRNA synthetase, mitochondrial		PRO_0000122200
Regions
	Nucleotide binding	284 – 286	3	ATP By similarity
	Nucleotide binding	371 – 374	4	ATP By similarity
	Region	251 – 253	3	Serine binding By similarity
Sites
	Binding site	300	1	ATP; via carbonyl oxygen and amide nitrogen By similarity
	Binding site	307	1	Serine By similarity
	Binding site	407	1	Serine By similarity

Sequences

	Sequence		Length	Mass (Da)
	P38705-1 [UniParc]. Last modified February 1, 1995. Version 1. Checksum: 2C0AE2CB644E7C54	FASTA	446	50,390
	10 20 30 40 50 60 MIIRRLFSIS NRSFFLKKPQ FDVKKIIEMI PQYQTSIQNR ELIEADSIIR SLQLLGERYQ 70 80 90 100 110 120 NIKEIDKVIA DIQIQRKSIE AQIKKDKTKI TEYSAALKAL KEQYNDQNSK SSELKKKILE 130 140 150 160 170 180 TCKSLPNTLD PTVPLDAPQI EQWINPLKTH KTSEAQAHVD IMLKKNMLDL QTASNVTGMS 190 200 210 220 230 240 WYYLLNDGAR LEQALVAYAL KKANENGFSS CVPPSITKKE LIDACGFNPR DMNNERQIYA 250 260 270 280 290 300 LQDTNLGLVA TAEIPLAGLG ANKVLELNSG ECSKKLVGVS RCYRAEAGAR GKDTKGLYRV 310 320 330 340 350 360 HEFTKVELFC WSKPETSAKV LEEIKQFQIS VVEELGIPAK VLNMPSNDLG NPAFKKYDIE 370 380 390 400 410 420 AWMPGRGKFG EISSASNCTD FQSRRLNTKY RDDNTGKLEY VHTLNGTAMA IPRVIVALVE 430 440 NFYDPSTGKI SVPECLREFM NGQRYI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete nucleotide sequence of Saccharomyces cerevisiae chromosome VIII." Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Dover J., Du Z., Favello A., Fulton L., Gattung S., Geisel C., Kirsten J., Kucaba T., Hillier L.W., Jier M., Johnston L., Langston Y., Latreille P. , Louis E.J., Macri C., Mardis E., Menezes S., Mouser L., Nhan M., Rifkin L., Riles L., St Peter H., Trevaskis E., Vaughan K., Vignati D., Wilcox L., Wohldman P., Waterston R., Wilson R., Vaudin M. Science 265:2077-2082(1994) [PubMed: 8091229] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed: 17322287] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[3]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed: 14562095] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[4]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U10400 Genomic DNA. Translation: AAB68940.1. AY558105 Genomic DNA. Translation: AAS56431.1.
PIR	S46786.
RefSeq	NP_011875.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:5573N.
IntAct	P38705. 24 interactions.
STRING	P38705.
Proteomic databases
PRIDE	P38705.
Genome annotation databases
Ensembl	YHR011W; YHR011W; YHR011W; Saccharomyces cerevisiae. [Genome view]
GeneID	856402.
GenomeReviews	Gene locus YHR011W in contig U00093_GR.
KEGG	sce:YHR011W.
NMPDR	fig|4932.3.peg.3020.
Organism-specific databases
CYGD	YHR011w.
SGD	S000001053. DIA4.
Phylogenomic databases
HOGENOM	P38705.
OMA	GSHSDER.
Enzyme and pathway databases
BRENDA	6.1.1.11. 250.
Gene expression databases
ArrayExpress	P38705.
Genevestigator	P38705.
GermOnline	YHR011W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR002314. aa-tRNA-synt_IIb_cons-reg. IPR006195. aa-tRNA-synth_II_cons-reg. IPR002317. Ser-tRNA-synth_IIa. IPR018156. Ser-tRNA-synth_IIa_C. [Graphical view]
PANTHER	PTHR11778. tRNA-synt_ser. 1 hit.
Pfam	PF00587. tRNA-synt_2b. 1 hit. [Graphical view]
PIRSF	PIRSF001529. Ser-tRNA-synth_IIa. 1 hit.
PRINTS	PR00981. TRNASYNTHSER.
TIGRFAMs	TIGR00414. serS. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	981937.

Entry information

Entry name

SYSM_YEAST

Accession

Primary (citable) accession number: P38705

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	February 1, 1995
Last modified:	November 3, 2009
This is version 80 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)