Reviewed,
UniProtKB/Swiss-Prot P38704 (STP2_YEAST)
Last modified
November 24, 2009.
Version 77.
History...
Clusters with 100%,
90%,
50% identity |
Documents (3) |
Third-party data |
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Names and origin
| Protein names | Recommended name: Transcription factor STP2 | ||||
| Gene names |
| ||||
| Organism | Saccharomyces cerevisiae (Baker's yeast) [Complete proteome] | ||||
| Taxonomic identifier | 4932 [NCBI] | ||||
| Taxonomic lineage | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces |
Protein attributes
| Sequence length | 541 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is further processed into a mature form. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Function | Transcription factor involved in the regulation of gene expression in response to extracellular amino acid levels. Synthesized as latent cytoplasmic precursor, which, upon a signal initiated by the plasma membrane SPS (SSY1-PTR3-SSY5) amino acid sensor system, becomes proteolytically activated and relocates to the nucleus, where it induces the expression of SPS-sensor-regulated genes, including the amino-acid permeases BAP2 and BAP3. Binding to promoters is facilitated by DAL81 By similarity. Involved in the repression of genes subject to nitrogen catabolite repression and genes involved in stress response. Negatively regulated by inner nuclear membrane proteins ASI1, ASI2 and ASI3, which prevent unprocessed precursor forms that escape cytoplasmic anchoring from inducing SPS-sensor-regulated genes. |
| Subunit structure | Interacts (via Region II) with SSY5; protease component of the SPS-sensor. |
| Subcellular location | Cell membrane; Peripheral membrane protein; Cytoplasmic side By similarity. Nucleus. Note: Localizes to the cytoplasm in its unprocessed form and is targeted to the nucleus after proteolytic processing upon induction by amino acids. |
| Domain | The N-terminal inhibitory domain contains conserved sequence elements important for cytoplasmic retention (Region I) and proteolytic processing (Region II) of the protein By similarity. Region I is also required for ASI1/2/3-mediated negative regulation of transcription. |
| Post-translational modification | Activated by the amino acid-induced proteolytic removal of an N-terminal inhibitory domain by serine protease SSY5, an intrinsic component of the SPS-sensor. Processing requires at least 2 components of the SCF(GRR1) ubiquitin ligase complex, namely the F-box protein GRR1 and the E2 enzyme CDC34, but does not depend on the proteasome. Processing is negatively regulated by the protein phosphatase 2A regulatory subunit RTS1 By similarity. |
| Miscellaneous | Present with 6490 molecules/cell in log phase SD medium. Ref.7 |
| Sequence similarities | Contains 3 C2H2-type zinc fingers. |
Ontologies
| Keywords | |
|---|---|
| Cellular component | Cell membrane Membrane Nucleus |
| Domain | Repeat Zinc-finger |
| Ligand | DNA-binding Metal-binding Zinc |
| PTM | Phosphoprotein Zymogen |
| Technical term | Complete proteome |
| Gene Ontology (GO) | |
| Biological process | positive regulation of transcription from RNA polymerase II promoter Ref.3 Inferred from genetic interaction. Source: SGD |
| Cellular component | nucleus Ref.3 Inferred from physical interaction. Source: SGD plasma membraneInferred from electronic annotation. Source: UniProtKB-KW |
| Molecular function | protein binding Inferred from physical interaction. Source: IntAct sequence-specific DNA bindingInferred from direct assay. Source: SGD specific RNA polymerase II transcription factor activity Ref.3Inferred from genetic interaction. Source: SGD zinc ion bindingInferred from electronic annotation. Source: UniProtKB-KW |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Propeptide | 1 – ? | PRO_0000377649 | |||||||
| Chain | ? – 541 | Transcription factor STP2 | PRO_0000046854 | ||||||
Regions | |||||||||
| Zinc finger | 204 – 226 | 23 | C2H2-type 1 | ||||||
| Zinc finger | 232 – 267 | 36 | C2H2-type 2; atypical | ||||||
| Zinc finger | 284 – 309 | 26 | C2H2-type 3; atypical | ||||||
| Region | 13 – 32 | 20 | I | ||||||
| Region | 73 – 105 | 33 | II | ||||||
Amino acid modifications | |||||||||
| Modified residue | 143 | 1 | Phosphoserine Ref.12 | ||||||
| Modified residue | 459 | 1 | Phosphoserine Ref.12 | ||||||
Experimental info | |||||||||
| Mutagenesis | 2 – 74 | 73 | Missing: Dominant active transcription factor. Ref.5 | ||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "Complete nucleotide sequence of Saccharomyces cerevisiae chromosome VIII." Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Dover J., Du Z., Favello A., Fulton L., Gattung S., Geisel C., Kirsten J., Kucaba T., Hillier L.W., Jier M., Johnston L., Langston Y., Latreille P. Vaudin M.Science 265:2077-2082(1994) [PubMed: 8091229] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972. |
| [2] | "Variations of the C2H2 zinc finger motif in the yeast genome and classification of yeast zinc finger proteins." Boehm S., Frishman D., Mewes H.-W. Nucleic Acids Res. 25:2464-2469(1997) [PubMed: 9171100] [Abstract] Cited for: DOMAIN ATYPICAL ZINC FINGER. |
| [3] | "Stp1p, Stp2p and Abf1p are involved in regulation of expression of the amino acid transporter gene BAP3 of Saccharomyces cerevisiae." de Boer M., Nielsen P.S., Bebelman J.-P., Heerikhuizen H., Andersen H.A., Planta R.J. Nucleic Acids Res. 28:974-981(2000) [PubMed: 10648791] [Abstract] Cited for: FUNCTION IN TRANSCRIPTION REGULATION. |
| [4] | "Transcriptional regulation of the Saccharomyces cerevisiae amino acid permease gene BAP2." Nielsen P.S., van den Hazel B., Didion T., de Boer M., Joergensen M.U., Planta R.J., Kielland-Brandt M.C., Andersen H.A. Mol. Gen. Genet. 264:613-622(2001) [PubMed: 11212916] [Abstract] Cited for: DNA BINDING. |
| [5] | "Receptor-mediated endoproteolytic activation of two transcription factors in yeast." Andreasson C., Ljungdahl P.O. Genes Dev. 16:3158-3172(2002) [PubMed: 12502738] [Abstract] Cited for: PROTEOLYTIC PROCESSING, MUTAGENESIS OF 2-PRO--PHE-74, SUBCELLULAR LOCATION. |
| [6] | "Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed: 14562095] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS]. |
| [7] | "Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS]. |
| [8] | "The N-terminal regulatory domain of Stp1p is modular and, fused to an artificial transcription factor, confers full Ssy1p-Ptr3p-Ssy5p sensor control." Andreasson C., Ljungdahl P.O. Mol. Cell. Biol. 24:7503-7513(2004) [PubMed: 15314160] [Abstract] Cited for: DOMAIN. |
| [9] | "Transcriptional profiling of extracellular amino acid sensing in Saccharomyces cerevisiae and the role of Stp1p and Stp2p." Eckert-Boulet N., Nielsen P.S., Friis C., dos Santos M.M., Nielsen J., Kielland-Brandt M.C., Regenberg B. Yeast 21:635-648(2004) [PubMed: 15197729] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION. |
| [10] | "Asi1 is an inner nuclear membrane protein that restricts promoter access of two latent transcription factors." Boban M., Zargari A., Andreasson C., Heessen S., Thyberg J., Ljungdahl P.O. J. Cell Biol. 173:695-707(2006) [PubMed: 16735580] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION. |
| [11] | "Inner nuclear membrane proteins Asi1, Asi2, and Asi3 function in concert to maintain the latent properties of transcription factors Stp1 and Stp2." Zargari A., Boban M., Heessen S., Andreasson C., Thyberg J., Ljungdahl P.O. J. Biol. Chem. 282:594-605(2007) [PubMed: 17085444] [Abstract] Cited for: FUNCTION. |
| [12] | "A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-143 AND SER-459, MASS SPECTROMETRY. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |
|---|---|
| U10555 Genomic DNA. Translation: AAB68423.1. | |
| PIR | S46794. |
| RefSeq | NP_011870.1. |
3D structure databases | |
| ModBase | Search... |
Protein-protein interaction databases | |
| IntAct | P38704. 7 interactions. |
| STRING | P38704. |
Genome annotation databases | |
| Ensembl | YHR006W; YHR006W; YHR006W; Saccharomyces cerevisiae. [Genome view] |
| GeneID | 856397. |
| KEGG | sce:YHR006W. |
| NMPDR | fig|4932.3.peg.3014. |
Organism-specific databases | |
| CYGD | YHR006w. |
| SGD | S000001048. STP2. |
Phylogenomic databases | |
| HOGENOM | P38704. |
| OrthoDB | EOG9H1CBT |
Gene expression databases | |
| ArrayExpress | P38704. |
| Genevestigator | P38704. |
| GermOnline | YHR006W. Saccharomyces cerevisiae. |
Family and domain databases | |
| InterPro | IPR007087. Znf_C2H2. IPR015880. Znf_C2H2-like. [Graphical view] |
| Pfam | PF00096. zf-C2H2. 1 hit. [Graphical view] |
| SMART | SM00355. ZnF_C2H2. 2 hits. [Graphical view] |
| PROSITE | PS00028. ZINC_FINGER_C2H2_1. 1 hit. PS50157. ZINC_FINGER_C2H2_2. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Other Resources | |
| NextBio | 981922. |
Entry information
| Entry name | STP2_YEAST | ||||||||
| Accession | Primary (citable) accession number: P38704 | ||||||||
| Entry history |
| ||||||||
| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | FPAP (Fungal Proteome Annotation Project) | ||||||||
Relevant documents
| SIMILARITY comments Index of protein domains and families |
| Yeast Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD |
| Yeast chromosome VIII Yeast (Saccharomyces cerevisiae) chromosome VIII: entries and gene names |

Clusters with


