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Protein

Transcription factor STP2

Gene

STP2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Transcription factor involved in the regulation of gene expression in response to extracellular amino acid levels. Synthesized as latent cytoplasmic precursor, which, upon a signal initiated by the plasma membrane SPS (SSY1-PTR3-SSY5) amino acid sensor system, becomes proteolytically activated and relocates to the nucleus, where it induces the expression of SPS-sensor-regulated genes, including the amino-acid permeases BAP2 and BAP3. Binding to promoters is facilitated by DAL81 (By similarity). Involved in the repression of genes subject to nitrogen catabolite repression and genes involved in stress response. Negatively regulated by inner nuclear membrane proteins ASI1, ASI2 and ASI3, which prevent unprocessed precursor forms that escape cytoplasmic anchoring from inducing SPS-sensor-regulated genes.By similarity4 Publications

Miscellaneous

Present with 6490 molecules/cell in log phase SD medium.1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri204 – 226C2H2-type 1PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri232 – 267C2H2-type 2; atypicalPROSITE-ProRule annotationAdd BLAST36
Zinc fingeri284 – 309C2H2-type 3; atypicalPROSITE-ProRule annotationAdd BLAST26

GO - Molecular functioni

GO - Biological processi

  • positive regulation of transcription by RNA polymerase II Source: SGD

Keywordsi

Molecular functionDNA-binding
LigandMetal-binding, Zinc

Enzyme and pathway databases

BioCyciYEAST:G3O-31071-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Transcription factor STP2
Gene namesi
Name:STP2
Ordered Locus Names:YHR006W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VIII

Organism-specific databases

EuPathDBiFungiDB:YHR006W
SGDiS000001048 STP2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell membrane, Membrane, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi2 – 74Missing : Dominant active transcription factor. 1 PublicationAdd BLAST73

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_0000046854? – 541Transcription factor STP2
PropeptideiPRO_00003776491 – ?

Post-translational modificationi

Activated by the amino acid-induced proteolytic removal of an N-terminal inhibitory domain by serine protease SSY5, an intrinsic component of the SPS-sensor. Processing requires at least 2 components of the SCF(GRR1) ubiquitin ligase complex, namely the F-box protein GRR1 and the E2 enzyme CDC34, but does not depend on the proteasome. Processing is negatively regulated by the protein phosphatase 2A regulatory subunit RTS1 (By similarity).By similarity

Keywords - PTMi

Zymogen

Proteomic databases

MaxQBiP38704
PaxDbiP38704
PRIDEiP38704

PTM databases

iPTMnetiP38704

Interactioni

Subunit structurei

Interacts (via Region II) with SSY5; protease component of the SPS-sensor.

Protein-protein interaction databases

BioGridi36433, 106 interactors
IntActiP38704, 5 interactors
MINTiP38704
STRINGi4932.YHR006W

Structurei

3D structure databases

ProteinModelPortaliP38704
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni13 – 32IAdd BLAST20
Regioni73 – 105IIAdd BLAST33

Domaini

The N-terminal inhibitory domain contains conserved sequence elements important for cytoplasmic retention (Region I) and proteolytic processing (Region II) of the protein (By similarity). Region I is also required for ASI1/2/3-mediated negative regulation of transcription.By similarity2 Publications

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri204 – 226C2H2-type 1PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri232 – 267C2H2-type 2; atypicalPROSITE-ProRule annotationAdd BLAST36
Zinc fingeri284 – 309C2H2-type 3; atypicalPROSITE-ProRule annotationAdd BLAST26

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

GeneTreeiENSGT00390000007162
HOGENOMiHOG000141772
InParanoidiP38704
OrthoDBiEOG092C3MIG

Family and domain databases

InterProiView protein in InterPro
IPR036236 Znf_C2H2_sf
IPR013087 Znf_C2H2_type
SMARTiView protein in SMART
SM00355 ZnF_C2H2, 2 hits
SUPFAMiSSF57667 SSF57667, 1 hit
PROSITEiView protein in PROSITE
PS00028 ZINC_FINGER_C2H2_1, 1 hit
PS50157 ZINC_FINGER_C2H2_2, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P38704-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPILSLSSTR NSVLTRIYDY LKALVQQVIV PNVEDDKSSK STPFEKLEPA
60 70 80 90 100
KQNHPQKDCC ATEKDDLVDV SELFPKQNNK QLSLTSKSSV VPCALNLDNL
110 120 130 140 150
ETPFSIKIDN NGAVTTQLNL DEPILRGPSR GEPAKLQNDL ISSPPLEESY
160 170 180 190 200
INNDQYKALF PSNFLPITPV SSVITPASKK SIDESPLSDE VQGIADESSE
210 220 230 240 250
TLPYICHYCD ARFRIRGYLT RHIKKHAKRK AYHCPFFDNS ISQELRCHTS
260 270 280 290 300
GGFSRRDTYK THLKSRHFTY PEGVKPQDRN KSPGVCTQCG EHFSTSESWV
310 320 330 340 350
ENHIEAGSCK GLPEGYSEGI REKKKTSKMK MIKTSDGQTR FISSDESVSE
360 370 380 390 400
PALQNKNCIE ATVMQSKERP NDKIIPTKTE KNDFGIGTQW FERKQISRPT
410 420 430 440 450
QTTQSRGPTE VQNLKEWSII SPPILSPQNA SSVPQEYQSS RYTLHMDSPA
460 470 480 490 500
LSSASSALSP LSGDPITTTE TNKSYPLDSE QSLLEPDKTE EDAINQSKES
510 520 530 540
NMISINEMLQ KQMDFELLGE NHLKETQDYL ALYKKAYGIE F
Length:541
Mass (Da):60,792
Last modified:February 1, 1995 - v1
Checksum:iBAF84576CE07180C
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti101E → G in AAU09738 (PubMed:17322287).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U10555 Genomic DNA Translation: AAB68423.1
AY723821 Genomic DNA Translation: AAU09738.1
BK006934 Genomic DNA Translation: DAA06694.1
PIRiS46794
RefSeqiNP_011870.1, NM_001179136.1

Genome annotation databases

EnsemblFungiiYHR006W; YHR006W; YHR006W
GeneIDi856397
KEGGisce:YHR006W

Similar proteinsi

Entry informationi

Entry nameiSTP2_YEAST
AccessioniPrimary (citable) accession number: P38704
Secondary accession number(s): D3DKV0, E9P956
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: March 28, 2018
This is version 151 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health