P38704 (STP2_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Transcription factor STP2

Gene names

Name:	STP2
Ordered Locus Names:	YHR006W

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	541 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Transcription factor involved in the regulation of gene expression in response to extracellular amino acid levels. Synthesized as latent cytoplasmic precursor, which, upon a signal initiated by the plasma membrane SPS (SSY1-PTR3-SSY5) amino acid sensor system, becomes proteolytically activated and relocates to the nucleus, where it induces the expression of SPS-sensor-regulated genes, including the amino-acid permeases BAP2 and BAP3. Binding to promoters is facilitated by DAL81 By similarity. Involved in the repression of genes subject to nitrogen catabolite repression and genes involved in stress response. Negatively regulated by inner nuclear membrane proteins ASI1, ASI2 and ASI3, which prevent unprocessed precursor forms that escape cytoplasmic anchoring from inducing SPS-sensor-regulated genes. Ref.5 Ref.11 Ref.12 Ref.13
Subunit structure	Interacts (via Region II) with SSY5; protease component of the SPS-sensor.
Subcellular location	Cell membrane; Peripheral membrane protein; Cytoplasmic side By similarity. Nucleus. Note: Localizes to the cytoplasm in its unprocessed form and is targeted to the nucleus after proteolytic processing upon induction by amino acids. Ref.7 Ref.8 Ref.11 Ref.12
Domain	The N-terminal inhibitory domain contains conserved sequence elements important for cytoplasmic retention (Region I) and proteolytic processing (Region II) of the protein By similarity. Region I is also required for ASI1/2/3-mediated negative regulation of transcription. Ref.4 Ref.10
Post-translational modification	Activated by the amino acid-induced proteolytic removal of an N-terminal inhibitory domain by serine protease SSY5, an intrinsic component of the SPS-sensor. Processing requires at least 2 components of the SCF(GRR1) ubiquitin ligase complex, namely the F-box protein GRR1 and the E2 enzyme CDC34, but does not depend on the proteasome. Processing is negatively regulated by the protein phosphatase 2A regulatory subunit RTS1 By similarity.
Miscellaneous	Present with 6490 molecules/cell in log phase SD medium. Ref.9
Sequence similarities	Contains 3 C2H2-type zinc fingers.

Ontologies

Keywords
Cellular component	Cell membrane Membrane Nucleus
Domain	Repeat Zinc-finger
Ligand	DNA-binding Metal-binding Zinc
PTM	Phosphoprotein Zymogen
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular component	cytoplasm Inferred from direct assay. Source: SGD nucleus Inferred from direct assay. Source: SGD plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	RNA polymerase II core promoter proximal region sequence-specific DNA binding Inferred from direct assay Ref.6. Source: SGD RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription Inferred from direct assay. Source: SGD zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Propeptide	1 – ?				PRO_0000377649
	Chain	? – 541		Transcription factor STP2		PRO_0000046854
Regions
	Zinc finger	204 – 226	23	C2H2-type 1
	Zinc finger	232 – 267	36	C2H2-type 2; atypical
	Zinc finger	284 – 309	26	C2H2-type 3; atypical
	Region	13 – 32	20	I
	Region	73 – 105	33	II
Amino acid modifications
	Modified residue	143	1	Phosphoserine Ref.14
	Modified residue	459	1	Phosphoserine Ref.14
Experimental info
	Mutagenesis	2 – 74	73	Missing: Dominant active transcription factor. Ref.7
	Sequence conflict	101	1	E → G in AAU09738. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	P38704 [UniParc]. Last modified February 1, 1995. Version 1. Checksum: BAF84576CE07180C	FASTA	541	60,792
	10 20 30 40 50 60 MPILSLSSTR NSVLTRIYDY LKALVQQVIV PNVEDDKSSK STPFEKLEPA KQNHPQKDCC 70 80 90 100 110 120 ATEKDDLVDV SELFPKQNNK QLSLTSKSSV VPCALNLDNL ETPFSIKIDN NGAVTTQLNL 130 140 150 160 170 180 DEPILRGPSR GEPAKLQNDL ISSPPLEESY INNDQYKALF PSNFLPITPV SSVITPASKK 190 200 210 220 230 240 SIDESPLSDE VQGIADESSE TLPYICHYCD ARFRIRGYLT RHIKKHAKRK AYHCPFFDNS 250 260 270 280 290 300 ISQELRCHTS GGFSRRDTYK THLKSRHFTY PEGVKPQDRN KSPGVCTQCG EHFSTSESWV 310 320 330 340 350 360 ENHIEAGSCK GLPEGYSEGI REKKKTSKMK MIKTSDGQTR FISSDESVSE PALQNKNCIE 370 380 390 400 410 420 ATVMQSKERP NDKIIPTKTE KNDFGIGTQW FERKQISRPT QTTQSRGPTE VQNLKEWSII 430 440 450 460 470 480 SPPILSPQNA SSVPQEYQSS RYTLHMDSPA LSSASSALSP LSGDPITTTE TNKSYPLDSE 490 500 510 520 530 540 QSLLEPDKTE EDAINQSKES NMISINEMLQ KQMDFELLGE NHLKETQDYL ALYKKAYGIE F

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete nucleotide sequence of Saccharomyces cerevisiae chromosome VIII." Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Dover J., Du Z., Favello A., Fulton L., Gattung S., Geisel C., Kirsten J., Kucaba T., Hillier L.W., Jier M., Johnston L., Langston Y., Latreille P. , Louis E.J., Macri C., Mardis E., Menezes S., Mouser L., Nhan M., Rifkin L., Riles L., St Peter H., Trevaskis E., Vaughan K., Vignati D., Wilcox L., Wohldman P., Waterston R., Wilson R., Vaudin M. Science 265:2077-2082(1994) [PubMed: 8091229] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[3]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed: 17322287] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[4]	"Variations of the C2H2 zinc finger motif in the yeast genome and classification of yeast zinc finger proteins." Boehm S., Frishman D., Mewes H.-W. Nucleic Acids Res. 25:2464-2469(1997) [PubMed: 9171100] [Abstract] Cited for: DOMAIN ATYPICAL ZINC FINGER.
[5]	"Stp1p, Stp2p and Abf1p are involved in regulation of expression of the amino acid transporter gene BAP3 of Saccharomyces cerevisiae." de Boer M., Nielsen P.S., Bebelman J.-P., Heerikhuizen H., Andersen H.A., Planta R.J. Nucleic Acids Res. 28:974-981(2000) [PubMed: 10648791] [Abstract] Cited for: FUNCTION IN TRANSCRIPTION REGULATION.
[6]	"Transcriptional regulation of the Saccharomyces cerevisiae amino acid permease gene BAP2." Nielsen P.S., van den Hazel B., Didion T., de Boer M., Joergensen M.U., Planta R.J., Kielland-Brandt M.C., Andersen H.A. Mol. Gen. Genet. 264:613-622(2001) [PubMed: 11212916] [Abstract] Cited for: DNA BINDING.
[7]	"Receptor-mediated endoproteolytic activation of two transcription factors in yeast." Andreasson C., Ljungdahl P.O. Genes Dev. 16:3158-3172(2002) [PubMed: 12502738] [Abstract] Cited for: PROTEOLYTIC PROCESSING, MUTAGENESIS OF 2-PRO--PHE-74, SUBCELLULAR LOCATION.
[8]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed: 14562095] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[9]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[10]	"The N-terminal regulatory domain of Stp1p is modular and, fused to an artificial transcription factor, confers full Ssy1p-Ptr3p-Ssy5p sensor control." Andreasson C., Ljungdahl P.O. Mol. Cell. Biol. 24:7503-7513(2004) [PubMed: 15314160] [Abstract] Cited for: DOMAIN.
[11]	"Transcriptional profiling of extracellular amino acid sensing in Saccharomyces cerevisiae and the role of Stp1p and Stp2p." Eckert-Boulet N., Nielsen P.S., Friis C., dos Santos M.M., Nielsen J., Kielland-Brandt M.C., Regenberg B. Yeast 21:635-648(2004) [PubMed: 15197729] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION.
[12]	"Asi1 is an inner nuclear membrane protein that restricts promoter access of two latent transcription factors." Boban M., Zargari A., Andreasson C., Heessen S., Thyberg J., Ljungdahl P.O. J. Cell Biol. 173:695-707(2006) [PubMed: 16735580] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION.
[13]	"Inner nuclear membrane proteins Asi1, Asi2, and Asi3 function in concert to maintain the latent properties of transcription factors Stp1 and Stp2." Zargari A., Boban M., Heessen S., Andreasson C., Thyberg J., Ljungdahl P.O. J. Biol. Chem. 282:594-605(2007) [PubMed: 17085444] [Abstract] Cited for: FUNCTION.
[14]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-143 AND SER-459, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U10555 Genomic DNA. Translation: AAB68423.1. AY723821 Genomic DNA. Translation: AAU09738.1. BK006934 Genomic DNA. Translation: DAA06694.1.
PIR	S46794.
RefSeq	NP_011870.1. NM_001179136.1.
3D structure databases
ProteinModelPortal	P38704.
SMR	P38704. Positions 190-334.
ModBase	Search...
Protein-protein interaction databases
IntAct	P38704. 5 interactions.
MINT	MINT-1324111.
STRING	P38704.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YHR006W; YHR006W; YHR006W.
GeneID	856397.
KEGG	sce:YHR006W.
NMPDR	fig|4932.3.peg.3014.
Organism-specific databases
CYGD	YHR006w.
SGD	S000001048. STP2.
Phylogenomic databases
eggNOG	fuNOG08493.
HOGENOM	HBG203844.
OrthoDB	EOG4K3Q5F.
Gene expression databases
ArrayExpress	P38704.
Genevestigator	P38704.
GermOnline	YHR006W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR007087. Znf_C2H2. IPR015880. Znf_C2H2-like. IPR013087. Znf_C2H2/integrase_DNA-bd. [Graphical view]
Gene3D	G3DSA:3.30.160.60. Znf_C2H2/integrase_DNA-bd. 1 hit.
Pfam	PF00096. zf-C2H2. 1 hit. [Graphical view]
SMART	SM00355. ZnF_C2H2. 2 hits. [Graphical view]
PROSITE	PS00028. ZINC_FINGER_C2H2_1. 1 hit. PS50157. ZINC_FINGER_C2H2_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	981922.

Entry information

Entry name

STP2_YEAST

Accession

Primary (citable) accession number: P38704
Secondary accession number(s): D3DKV0, E9P956

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	February 1, 1995
Last modified:	January 25, 2012
This is version 100 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program