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Protein

Transcription factor STP2

Gene

STP2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Transcription factor involved in the regulation of gene expression in response to extracellular amino acid levels. Synthesized as latent cytoplasmic precursor, which, upon a signal initiated by the plasma membrane SPS (SSY1-PTR3-SSY5) amino acid sensor system, becomes proteolytically activated and relocates to the nucleus, where it induces the expression of SPS-sensor-regulated genes, including the amino-acid permeases BAP2 and BAP3. Binding to promoters is facilitated by DAL81 (By similarity). Involved in the repression of genes subject to nitrogen catabolite repression and genes involved in stress response. Negatively regulated by inner nuclear membrane proteins ASI1, ASI2 and ASI3, which prevent unprocessed precursor forms that escape cytoplasmic anchoring from inducing SPS-sensor-regulated genes.By similarity4 Publications

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri204 – 226C2H2-type 1PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri232 – 267C2H2-type 2; atypicalPROSITE-ProRule annotationAdd BLAST36
Zinc fingeri284 – 309C2H2-type 3; atypicalPROSITE-ProRule annotationAdd BLAST26

GO - Molecular functioni

GO - Biological processi

  • positive regulation of transcription from RNA polymerase II promoter Source: SGD
Complete GO annotation...

Keywords - Ligandi

DNA-binding, Metal-binding, Zinc

Enzyme and pathway databases

BioCyciYEAST:G3O-31071-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Transcription factor STP2
Gene namesi
Name:STP2
Ordered Locus Names:YHR006W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VIII

Organism-specific databases

EuPathDBiFungiDB:YHR006W.
SGDiS000001048. STP2.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: SGD
  • nucleus Source: SGD
  • plasma membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi2 – 74Missing : Dominant active transcription factor. 1 PublicationAdd BLAST73

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_0000046854? – 541Transcription factor STP2
PropeptideiPRO_00003776491 – ?

Post-translational modificationi

Activated by the amino acid-induced proteolytic removal of an N-terminal inhibitory domain by serine protease SSY5, an intrinsic component of the SPS-sensor. Processing requires at least 2 components of the SCF(GRR1) ubiquitin ligase complex, namely the F-box protein GRR1 and the E2 enzyme CDC34, but does not depend on the proteasome. Processing is negatively regulated by the protein phosphatase 2A regulatory subunit RTS1 (By similarity).By similarity

Keywords - PTMi

Zymogen

Proteomic databases

MaxQBiP38704.
PRIDEiP38704.

PTM databases

iPTMnetiP38704.

Interactioni

Subunit structurei

Interacts (via Region II) with SSY5; protease component of the SPS-sensor.

Protein-protein interaction databases

BioGridi36433. 65 interactors.
IntActiP38704. 5 interactors.
MINTiMINT-1324111.

Structurei

3D structure databases

ProteinModelPortaliP38704.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni13 – 32IAdd BLAST20
Regioni73 – 105IIAdd BLAST33

Domaini

The N-terminal inhibitory domain contains conserved sequence elements important for cytoplasmic retention (Region I) and proteolytic processing (Region II) of the protein (By similarity). Region I is also required for ASI1/2/3-mediated negative regulation of transcription.By similarity2 Publications

Sequence similaritiesi

Contains 3 C2H2-type zinc fingers.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri204 – 226C2H2-type 1PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri232 – 267C2H2-type 2; atypicalPROSITE-ProRule annotationAdd BLAST36
Zinc fingeri284 – 309C2H2-type 3; atypicalPROSITE-ProRule annotationAdd BLAST26

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

GeneTreeiENSGT00390000007162.
HOGENOMiHOG000141772.
InParanoidiP38704.
OrthoDBiEOG092C3MIG.

Family and domain databases

Gene3Di3.30.160.60. 1 hit.
InterProiIPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
IPR013087. Znf_C2H2/integrase_DNA-bd.
[Graphical view]
SMARTiSM00355. ZnF_C2H2. 2 hits.
[Graphical view]
PROSITEiPS00028. ZINC_FINGER_C2H2_1. 1 hit.
PS50157. ZINC_FINGER_C2H2_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P38704-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPILSLSSTR NSVLTRIYDY LKALVQQVIV PNVEDDKSSK STPFEKLEPA
60 70 80 90 100
KQNHPQKDCC ATEKDDLVDV SELFPKQNNK QLSLTSKSSV VPCALNLDNL
110 120 130 140 150
ETPFSIKIDN NGAVTTQLNL DEPILRGPSR GEPAKLQNDL ISSPPLEESY
160 170 180 190 200
INNDQYKALF PSNFLPITPV SSVITPASKK SIDESPLSDE VQGIADESSE
210 220 230 240 250
TLPYICHYCD ARFRIRGYLT RHIKKHAKRK AYHCPFFDNS ISQELRCHTS
260 270 280 290 300
GGFSRRDTYK THLKSRHFTY PEGVKPQDRN KSPGVCTQCG EHFSTSESWV
310 320 330 340 350
ENHIEAGSCK GLPEGYSEGI REKKKTSKMK MIKTSDGQTR FISSDESVSE
360 370 380 390 400
PALQNKNCIE ATVMQSKERP NDKIIPTKTE KNDFGIGTQW FERKQISRPT
410 420 430 440 450
QTTQSRGPTE VQNLKEWSII SPPILSPQNA SSVPQEYQSS RYTLHMDSPA
460 470 480 490 500
LSSASSALSP LSGDPITTTE TNKSYPLDSE QSLLEPDKTE EDAINQSKES
510 520 530 540
NMISINEMLQ KQMDFELLGE NHLKETQDYL ALYKKAYGIE F
Length:541
Mass (Da):60,792
Last modified:February 1, 1995 - v1
Checksum:iBAF84576CE07180C
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti101E → G in AAU09738 (PubMed:17322287).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U10555 Genomic DNA. Translation: AAB68423.1.
AY723821 Genomic DNA. Translation: AAU09738.1.
BK006934 Genomic DNA. Translation: DAA06694.1.
PIRiS46794.
RefSeqiNP_011870.1. NM_001179136.1.

Genome annotation databases

EnsemblFungiiYHR006W; YHR006W; YHR006W.
GeneIDi856397.
KEGGisce:YHR006W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U10555 Genomic DNA. Translation: AAB68423.1.
AY723821 Genomic DNA. Translation: AAU09738.1.
BK006934 Genomic DNA. Translation: DAA06694.1.
PIRiS46794.
RefSeqiNP_011870.1. NM_001179136.1.

3D structure databases

ProteinModelPortaliP38704.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi36433. 65 interactors.
IntActiP38704. 5 interactors.
MINTiMINT-1324111.

PTM databases

iPTMnetiP38704.

Proteomic databases

MaxQBiP38704.
PRIDEiP38704.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYHR006W; YHR006W; YHR006W.
GeneIDi856397.
KEGGisce:YHR006W.

Organism-specific databases

EuPathDBiFungiDB:YHR006W.
SGDiS000001048. STP2.

Phylogenomic databases

GeneTreeiENSGT00390000007162.
HOGENOMiHOG000141772.
InParanoidiP38704.
OrthoDBiEOG092C3MIG.

Enzyme and pathway databases

BioCyciYEAST:G3O-31071-MONOMER.

Miscellaneous databases

PROiP38704.

Family and domain databases

Gene3Di3.30.160.60. 1 hit.
InterProiIPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
IPR013087. Znf_C2H2/integrase_DNA-bd.
[Graphical view]
SMARTiSM00355. ZnF_C2H2. 2 hits.
[Graphical view]
PROSITEiPS00028. ZINC_FINGER_C2H2_1. 1 hit.
PS50157. ZINC_FINGER_C2H2_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSTP2_YEAST
AccessioniPrimary (citable) accession number: P38704
Secondary accession number(s): D3DKV0, E9P956
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: November 2, 2016
This is version 141 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 6490 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome VIII
    Yeast (Saccharomyces cerevisiae) chromosome VIII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.