Sphingosine N-acyltransferase LAG1 - Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

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P38703 (LAG1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified December 14, 2011. Version 103. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Sphingosine N-acyltransferase LAG1
EC=2.3.1.24

Alternative name(s):
Longevity assurance factor 1
Longevity assurance gene 1 protein
Longevity assurance protein 1

Gene names

Name:	LAG1
Ordered Locus Names:	YHL003C

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	411 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Component of the ceramide synthase complex required for C26-CoA-dependent ceramide synthesis. Redundant with LAC1. Facilitates ER-to-Golgi transport of GPI-anchored proteins. Involved in the aging process. Deletion of LAG1 results in a pronounced increase (approximately 50%) in mean and in maximum life span. Ref.7 Ref.8 Ref.9 Ref.13 Ref.14
Catalytic activity	Acyl-CoA + sphingosine = CoA + N-acylsphingosine.
Subcellular location	Endoplasmic reticulum membrane; Multi-pass membrane protein By similarity Ref.11.
Induction	Expression is controlled by CBF1. Ref.12
Sequence similarities	Belongs to the sphingosine N-acyltransferase family. Contains 1 TLC (TRAM/LAG1/CLN8) domain.

Ontologies

Keywords
Biological process	Lipid metabolism Lipid synthesis
Cellular component	Endoplasmic reticulum Membrane
Domain	Transmembrane Transmembrane helix
Molecular function	Transferase
PTM	Glycoprotein Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	ceramide biosynthetic process Inferred from direct assay Ref.14. Source: SGD replicative cell aging Inferred from mutant phenotype Ref.1. Source: SGD
Cellular component	endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	protein binding Inferred from physical interaction Ref.14. Source: IntAct sphingosine N-acyltransferase activity Inferred from direct assay Ref.14. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
LAC1	P28496	6	EBI-10035,EBI-26585
LIP1	Q03579	4	EBI-10035,EBI-27640

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 411

411

Sphingosine N-acyltransferase LAG1

PRO_0000185527

Regions

Topological domain

1 – 81

Cytoplasmic Potential

Transmembrane

82 – 102

Helical; Potential

Topological domain

103 – 134

Lumenal Potential

Transmembrane

135 – 155

Helical; Potential

Topological domain

156 – 176

Cytoplasmic Potential

Transmembrane

177 – 197

Helical; Potential

Topological domain

198 – 211

Lumenal Potential

Transmembrane

212 – 232

Helical; Potential

Topological domain

233 – 251

Cytoplasmic Potential

Transmembrane

252 – 272

Helical; Potential

Topological domain

273 – 296

Lumenal Potential

Transmembrane

297 – 317

Helical; Potential

Topological domain

318 – 355

Cytoplasmic Potential

Transmembrane

356 – 376

Helical; Potential

Topological domain

377 – 411

Lumenal Potential

Domain

168 – 384

217

TLC

Amino acid modifications

Modified residue

Phosphoserine Ref.18

Modified residue

Phosphoserine Ref.17

Modified residue

Phosphoserine Ref.17 Ref.18

Glycosylation

103

N-linked (GlcNAc...) Potential

Sequences

Sequence

Length

Mass (Da)

Tools

P38703 [UniParc].

Last modified February 1, 1995. Version 1.
Checksum: 91676D56AC053F3C
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FASTA

411

48,455

        10         20         30         40         50         60 
MTSATDKSID RLVVNAKTRR RNSSVGKIDL GDTVPGFAAM PESAASKNEA KKRMKALTGD 

        70         80         90        100        110        120 
SKKDSDLLWK VWFSYREMNY RHSWLTPFFI LVCVYSAYFL SGNRTESNPL HMFVAISYQV 

       130        140        150        160        170        180 
DGTDSYAKGI KDLSFVFFYM IFFTFLREFL MDVVIRPFTV YLNVTSEHRQ KRMLEQMYAI 

       190        200        210        220        230        240 
FYCGVSGPFG LYIMYHSDLW LFKTKPMYRT YPVITNPFLF KIFYLGQAAF WAQQACVLVL 

       250        260        270        280        290        300 
QLEKPRKDYK ELVFHHIVTL LLIWSSYVFH FTKMGLAIYI TMDVSDFFLS LSKTLNYLNS 

       310        320        330        340        350        360 
VFTPFVFGLF VFFWIYLRHV VNIRILWSVL TEFRHEGNYV LNFATQQYKC WISLPIVFVL 

       370        380        390        400        410 
IAALQLVNLY WLFLILRILY RLIWQGIQKD ERSDSDSDES AENEESKEKC E

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and characterization of LAG1, a longevity-assurance gene in yeast." D'Mello N.P., Childress A.M., Franklin D.S., Kale S.P., Pinswasdi C., Jazwinski S.M. J. Biol. Chem. 269:15451-15459(1994) [PubMed: 8195187] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 26786 / X2180-1A.
[2]	Jazwinski S.M. Submitted (MAR-1994) to the EMBL/GenBank/DDBJ databases Cited for: SEQUENCE REVISION TO 173-174; 220 AND 301-411.
[3]	Manske M., Sommer T., Prehn S., Rapoport T.A., Hartmann E. Submitted (JAN-1994) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[4]	"Complete nucleotide sequence of Saccharomyces cerevisiae chromosome VIII." Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Dover J., Du Z., Favello A., Fulton L., Gattung S., Geisel C., Kirsten J., Kucaba T., Hillier L.W., Jier M., Johnston L., Langston Y., Latreille P. Vaudin M. Science 265:2077-2082(1994) [PubMed: 8091229] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[5]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[6]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. LaBaer J. Genome Res. 17:536-543(2007) [PubMed: 17322287] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[7]	"Two endoplasmic reticulum (ER) membrane proteins that facilitate ER-to-Golgi transport of glycosylphosphatidylinositol-anchored proteins." Barz W.P., Walter P. Mol. Biol. Cell 10:1043-1059(1999) [PubMed: 10198056] [Abstract] Cited for: FUNCTION.
[8]	"C26-CoA-dependent ceramide synthesis of Saccharomyces cerevisiae is operated by Lag1p and Lac1p." Guillas I., Kirchman P.A., Chuard R., Pfefferli M., Jiang J.C., Jazwinski S.M., Conzelmann A. EMBO J. 20:2655-2665(2001) [PubMed: 11387200] [Abstract] Cited for: FUNCTION.
[9]	"Lag1p and Lac1p are essential for the acyl-CoA-dependent ceramide synthase reaction in Saccharomyces cerevisae." Schorling S., Vallee B., Barz W.P., Riezman H., Oesterhelt D. Mol. Biol. Cell 12:3417-3427(2001) [PubMed: 11694577] [Abstract] Cited for: FUNCTION.
[10]	"TRAM, LAG1 and CLN8: members of a novel family of lipid-sensing domains?" Winter E., Ponting C.P. Trends Biochem. Sci. 27:381-383(2002) [PubMed: 12151215] [Abstract] Cited for: DOMAIN, TOPOLOGY.
[11]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed: 14562095] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[12]	"Differential regulation of ceramide synthase components LAC1 and LAG1 in Saccharomyces cerevisiae." Kolaczkowski M., Kolaczkowska A., Gaigg B., Schneiter R., Moye-Rowley W.S. Eukaryot. Cell 3:880-892(2004) [PubMed: 15302821] [Abstract] Cited for: INDUCTION.
[13]	"Suppressor analysis points to the subtle role of the LAG1 ceramide synthase gene in determining yeast longevity." Jiang J.C., Kirchman P.A., Allen M., Jazwinski S.M. Exp. Gerontol. 39:999-1009(2004) [PubMed: 15236759] [Abstract] Cited for: FUNCTION.
[14]	"Lip1p: a novel subunit of acyl-CoA ceramide synthase." Vallee B., Riezman H. EMBO J. 24:730-741(2005) [PubMed: 15692566] [Abstract] Cited for: FUNCTION, MASS SPECTROMETRY, INTERACTION WITH LAC1 AND LIP1.
[15]	"Transmembrane topology of ceramide synthase in yeast." Kageyama-Yahara N., Riezman H. Biochem. J. 398:585-593(2006) [PubMed: 16756512] [Abstract] Cited for: TOPOLOGY.
[16]	"A global topology map of the Saccharomyces cerevisiae membrane proteome." Kim H., Melen K., Oesterberg M., von Heijne G. Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed: 16847258] [Abstract] Cited for: TOPOLOGY [LARGE SCALE ANALYSIS]. Strain: ATCC 208353 / W303-1A.
[17]	"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H. Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed: 17563356] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-23 AND SER-24, MASS SPECTROMETRY.
[18]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-8 AND SER-24, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U08133 Genomic DNA. Translation: AAA21579.1. U05335 Genomic DNA. Translation: AAF21442.1. U10555 Genomic DNA. Translation: AAB68429.1. AY558514 Genomic DNA. Translation: AAS56840.1. BK006934 Genomic DNA. Translation: DAA06684.1.
PIR	S46800.
RefSeq	NP_011860.1. NM_001179083.1.
3D structure databases
ProteinModelPortal	P38703.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-4393N.
IntAct	P38703. 15 interactions.
MINT	MINT-515520.
STRING	P38703.
Proteomic databases
PeptideAtlas	P38703.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YHL003C; YHL003C; YHL003C.
GeneID	856386.
KEGG	sce:YHL003C.
NMPDR	fig\|4932.3.peg.3003.
Organism-specific databases
CYGD	YHL003c.
SGD	S000000995. LAG1.
Phylogenomic databases
eggNOG	fuNOG05004.
GeneTree	EFGT00050000005520.
HOGENOM	HBG738741.
OMA	YKCWISQ.
OrthoDB	EOG447K3B.
Gene expression databases
ArrayExpress	P38703.
Genevestigator	P38703.
GermOnline	YHL003C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR016439. Longevity_assurance_LAG1_LAC1. IPR006634. TLC-dom. IPR013599. TRAM1. [Graphical view]
KO	K04709.
Pfam	PF08390. TRAM1. 1 hit. PF03798. TRAM_LAG1_CLN8. 1 hit. [Graphical view]
PIRSF	PIRSF005225. LAG1_LAC1. 1 hit.
SMART	SM00724. TLC. 1 hit. [Graphical view]
PROSITE	PS50922. TLC. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	981890.

Entry information

Entry name

LAG1_YEAST

Accession

Primary (citable) accession number: P38703
Secondary accession number(s): D3DKR1, Q6LD26

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	February 1, 1995
Last modified:	December 14, 2011
This is version 103 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome VIII

Yeast (Saccharomyces cerevisiae) chromosome VIII: entries and gene names

SIMILARITY comments

Index of protein domains and families

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents