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P38701

- RS20_YEAST

UniProt

P38701 - RS20_YEAST

Protein

40S ribosomal protein S20

Gene

RPS20

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
  1. Functioni

    GO - Molecular functioni

    1. RNA binding Source: InterPro
    2. structural constituent of ribosome Source: SGD

    GO - Biological processi

    1. cytoplasmic translation Source: SGD
    2. maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) Source: SGD

    Keywords - Molecular functioni

    Ribonucleoprotein, Ribosomal protein

    Enzyme and pathway databases

    BioCyciYEAST:G3O-31035-MONOMER.
    ReactomeiREACT_188965. SRP-dependent cotranslational protein targeting to membrane.
    REACT_189050. Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC).
    REACT_189183. Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC).
    REACT_217188. Formation of a pool of free 40S subunits.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    40S ribosomal protein S20
    Gene namesi
    Name:RPS20
    Synonyms:URP2
    Ordered Locus Names:YHL015W
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome VIII

    Organism-specific databases

    SGDiS000001007. RPS20.

    Subcellular locationi

    Cytoplasm By similarity

    GO - Cellular componenti

    1. cytosolic small ribosomal subunit Source: SGD

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11Removed3 Publications
    Chaini2 – 12112040S ribosomal protein S20PRO_0000146692Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21N-acetylserine3 Publications
    Cross-linki8 – 8Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)2 Publications

    Post-translational modificationi

    N-terminally acetylated by acetyltransferase NatA. Also partially acetylated by NatC.3 Publications

    Keywords - PTMi

    Acetylation, Isopeptide bond, Ubl conjugation

    Proteomic databases

    MaxQBiP38701.
    PaxDbiP38701.
    PeptideAtlasiP38701.
    PRIDEiP38701.

    Expressioni

    Gene expression databases

    GenevestigatoriP38701.

    Interactioni

    Subunit structurei

    Component of the small ribosomal subunit. Mature ribosomes consist of a small (40S) and a large (60S) subunit. The 40S subunit contains 32 different proteins (encoded by 56 genes) and 1 molecule of RNA (18S). The 60S subunit contains 46 different proteins (encoded by 81 genes) and 3 molecules of RNA (25S, 5.8S and 5S).1 Publication

    Protein-protein interaction databases

    BioGridi36408. 61 interactions.
    DIPiDIP-4060N.
    IntActiP38701. 10 interactions.
    MINTiMINT-539826.
    STRINGi4932.YHL015W.

    Structurei

    Secondary structure

    1
    121
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi22 – 298
    Helixi31 – 4717
    Beta strandi52 – 7322
    Beta strandi79 – 9315
    Helixi97 – 1059
    Beta strandi113 – 1164

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1K5Xmodel-J21-117[»]
    1S1Helectron microscopy11.70J21-117[»]
    1VW9electron microscopy6.10V1-121[»]
    1VWVelectron microscopy6.10V1-121[»]
    3IZBelectron microscopy-J1-121[»]
    3O2ZX-ray4.00N1-121[»]
    3O30X-ray4.00N1-121[»]
    3U5CX-ray3.00U1-121[»]
    3U5GX-ray3.00U1-121[»]
    4BYLelectron microscopy4.30U1-121[»]
    4BYTelectron microscopy6.60U1-121[»]
    4CUYelectron microscopy3.70U17-120[»]
    ProteinModelPortaliP38701.
    SMRiP38701. Positions 25-121.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP38701.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the ribosomal protein S10P family.Curated

    Phylogenomic databases

    eggNOGiCOG0051.
    GeneTreeiENSGT00390000003248.
    HOGENOMiHOG000270245.
    KOiK02969.
    OMAiWDRYELK.
    OrthoDBiEOG751NTH.

    Family and domain databases

    Gene3Di3.30.70.600. 1 hit.
    HAMAPiMF_00508. Ribosomal_S10.
    InterProiIPR001848. Ribosomal_S10.
    IPR018268. Ribosomal_S10_CS.
    IPR027486. Ribosomal_S10_dom.
    IPR005729. Ribosomal_S10_euk/arc.
    [Graphical view]
    PANTHERiPTHR11700. PTHR11700. 1 hit.
    PfamiPF00338. Ribosomal_S10. 1 hit.
    [Graphical view]
    PRINTSiPR00971. RIBOSOMALS10.
    SUPFAMiSSF54999. SSF54999. 1 hit.
    TIGRFAMsiTIGR01046. S10_Arc_S20_Euk. 1 hit.
    PROSITEiPS00361. RIBOSOMAL_S10. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P38701-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSDFQKEKVE EQEQQQQQII KIRITLTSTK VKQLENVSSN IVKNAEQHNL    50
    VKKGPVRLPT KVLKISTRKT PNGEGSKTWE TYEMRIHKRY IDLEAPVQIV 100
    KRITQITIEP GVDVEVVVAS N 121
    Length:121
    Mass (Da):13,907
    Last modified:January 23, 2007 - v3
    Checksum:i8B1607EDB63017F1
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z29089 Genomic DNA. Translation: CAA82331.1.
    U11582 Genomic DNA. Translation: AAB65068.1.
    BK006934 Genomic DNA. Translation: DAA06671.1.
    PIRiS46829.
    RefSeqiNP_011848.1. NM_001179095.1.

    Genome annotation databases

    EnsemblFungiiYHL015W; YHL015W; YHL015W.
    GeneIDi856371.
    KEGGisce:YHL015W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z29089 Genomic DNA. Translation: CAA82331.1 .
    U11582 Genomic DNA. Translation: AAB65068.1 .
    BK006934 Genomic DNA. Translation: DAA06671.1 .
    PIRi S46829.
    RefSeqi NP_011848.1. NM_001179095.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1K5X model - J 21-117 [» ]
    1S1H electron microscopy 11.70 J 21-117 [» ]
    1VW9 electron microscopy 6.10 V 1-121 [» ]
    1VWV electron microscopy 6.10 V 1-121 [» ]
    3IZB electron microscopy - J 1-121 [» ]
    3O2Z X-ray 4.00 N 1-121 [» ]
    3O30 X-ray 4.00 N 1-121 [» ]
    3U5C X-ray 3.00 U 1-121 [» ]
    3U5G X-ray 3.00 U 1-121 [» ]
    4BYL electron microscopy 4.30 U 1-121 [» ]
    4BYT electron microscopy 6.60 U 1-121 [» ]
    4CUY electron microscopy 3.70 U 17-120 [» ]
    ProteinModelPortali P38701.
    SMRi P38701. Positions 25-121.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 36408. 61 interactions.
    DIPi DIP-4060N.
    IntActi P38701. 10 interactions.
    MINTi MINT-539826.
    STRINGi 4932.YHL015W.

    Proteomic databases

    MaxQBi P38701.
    PaxDbi P38701.
    PeptideAtlasi P38701.
    PRIDEi P38701.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YHL015W ; YHL015W ; YHL015W .
    GeneIDi 856371.
    KEGGi sce:YHL015W.

    Organism-specific databases

    SGDi S000001007. RPS20.

    Phylogenomic databases

    eggNOGi COG0051.
    GeneTreei ENSGT00390000003248.
    HOGENOMi HOG000270245.
    KOi K02969.
    OMAi WDRYELK.
    OrthoDBi EOG751NTH.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-31035-MONOMER.
    Reactomei REACT_188965. SRP-dependent cotranslational protein targeting to membrane.
    REACT_189050. Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC).
    REACT_189183. Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC).
    REACT_217188. Formation of a pool of free 40S subunits.

    Miscellaneous databases

    EvolutionaryTracei P38701.
    NextBioi 981849.
    PROi P38701.

    Gene expression databases

    Genevestigatori P38701.

    Family and domain databases

    Gene3Di 3.30.70.600. 1 hit.
    HAMAPi MF_00508. Ribosomal_S10.
    InterProi IPR001848. Ribosomal_S10.
    IPR018268. Ribosomal_S10_CS.
    IPR027486. Ribosomal_S10_dom.
    IPR005729. Ribosomal_S10_euk/arc.
    [Graphical view ]
    PANTHERi PTHR11700. PTHR11700. 1 hit.
    Pfami PF00338. Ribosomal_S10. 1 hit.
    [Graphical view ]
    PRINTSi PR00971. RIBOSOMALS10.
    SUPFAMi SSF54999. SSF54999. 1 hit.
    TIGRFAMsi TIGR01046. S10_Arc_S20_Euk. 1 hit.
    PROSITEi PS00361. RIBOSOMAL_S10. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Suppression of yeast RNA polymerase III mutations by the URP2 gene encoding a protein homologous to the mammalian ribosomal protein S20."
      Hermann-Le Denmat S., Sipickzki M., Thuriaux P.
      J. Mol. Biol. 240:1-7(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 28383 / FL100 / VTT C-80102.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    4. Bienvenut W.V., Peters C.
      Submitted (MAY-2005) to UniProtKB
      Cited for: PROTEIN SEQUENCE OF 2-21; 33-43; 78-85 AND 90-102, CLEAVAGE OF INITIATOR METHIONINE, ACETYLATION AT SER-2, IDENTIFICATION BY MASS SPECTROMETRY.
    5. "The list of cytoplasmic ribosomal proteins of Saccharomyces cerevisiae."
      Planta R.J., Mager W.H.
      Yeast 14:471-477(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NOMENCLATURE, SUBUNIT.
    6. "The action of N-terminal acetyltransferases on yeast ribosomal proteins."
      Arnold R.J., Polevoda B., Reilly J.P., Sherman F.
      J. Biol. Chem. 274:37035-37040(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: CLEAVAGE OF INITIATOR METHIONINE, ACETYLATION AT SER-2 BY NATA.
    7. Cited for: UBIQUITINATION [LARGE SCALE ANALYSIS] AT LYS-8.
      Strain: SUB592.
    8. "A subset of membrane-associated proteins is ubiquitinated in response to mutations in the endoplasmic reticulum degradation machinery."
      Hitchcock A.L., Auld K., Gygi S.P., Silver P.A.
      Proc. Natl. Acad. Sci. U.S.A. 100:12735-12740(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: UBIQUITINATION [LARGE SCALE ANALYSIS] AT LYS-8.
    9. Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT SER-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    10. "Sites of ubiquitin attachment in Saccharomyces cerevisiae."
      Starita L.M., Lo R.S., Eng J.K., von Haller P.D., Fields S.
      Proteomics 12:236-240(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    11. "Structure of the 80S ribosome from Saccharomyces cerevisiae -- tRNA-ribosome and subunit-subunit interactions."
      Spahn C.M.T., Beckmann R., Eswar N., Penczek P.A., Sali A., Blobel G., Frank J.
      Cell 107:373-386(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: 3D-STRUCTURE MODELING OF 21-117, ELECTRON MICROSCOPY.
    12. "Domain movements of elongation factor eEF2 and the eukaryotic 80S ribosome facilitate tRNA translocation."
      Spahn C.M.T., Gomez-Lorenzo M.G., Grassucci R.A., Joergensen R., Andersen G.R., Beckmann R., Penczek P.A., Ballesta J.P.G., Frank J.
      EMBO J. 23:1008-1019(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: 3D-STRUCTURE MODELING OF 21-121, ELECTRON MICROSCOPY.

    Entry informationi

    Entry nameiRS20_YEAST
    AccessioniPrimary (citable) accession number: P38701
    Secondary accession number(s): D3DKP7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1995
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 130 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. Ribosomal proteins
      Ribosomal proteins families and list of entries
    3. SIMILARITY comments
      Index of protein domains and families
    4. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    5. Yeast chromosome VIII
      Yeast (Saccharomyces cerevisiae) chromosome VIII: entries and gene names

    External Data

    Dasty 3