40S ribosomal protein S20 - Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

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P38701 (RS20_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified December 14, 2011. Version 110. History...

Clusters with 100%, 90%, 50% identity |

Documents (5) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
40S ribosomal protein S20

Gene names

Name:	RPS20
Synonyms:	URP2
Ordered Locus Names:	YHL015W

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	121 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Subunit structure	Component of the small ribosomal subunit. Mature ribosomes consist of a small (40S) and a large (60S) subunit. The 40S subunit contains 32 different proteins (encoded by 56 genes) and 1 molecule of RNA (18S). The 60S subunit contains 46 different proteins (encoded by 81 genes) and 3 molecules of RNA (25S, 5.8S and 5S). Ref.6
Subcellular location	Cytoplasm By similarity.
Post-translational modification	N-terminally acetylated by acetyltransferase NatA. Also partially acetylated by NatC.
Sequence similarities	Belongs to the ribosomal protein S10P family.

Ontologies

Keywords
Cellular component	Cytoplasm
Molecular function	Ribonucleoprotein Ribosomal protein
PTM	Acetylation Isopeptide bond Phosphoprotein Ubl conjugation
Technical term	3D-structure Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological process	cytoplasmic translation Inferred by curator. Source: SGD maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) Inferred from mutant phenotype. Source: SGD
Cellular component	cytosolic small ribosomal subunit Inferred from direct assay. Source: SGD
Molecular function	RNA binding Inferred from electronic annotation. Source: InterPro structural constituent of ribosome Inferred by curator. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Initiator methionine

Removed Ref.4 Ref.7

Chain

2 – 121

120

40S ribosomal protein S20

PRO_0000146692

Amino acid modifications

Modified residue

N-acetylserine Ref.4 Ref.7 Ref.9

Modified residue

Phosphoserine Ref.9

Cross-link

Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) Ref.5 Ref.8

Sequences

Sequence

Length

Mass (Da)

Tools

P38701 [UniParc].

Last modified January 23, 2007. Version 3.
Checksum: 8B1607EDB63017F1
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FASTA

121

13,907

        10         20         30         40         50         60 
MSDFQKEKVE EQEQQQQQII KIRITLTSTK VKQLENVSSN IVKNAEQHNL VKKGPVRLPT 

        70         80         90        100        110        120 
KVLKISTRKT PNGEGSKTWE TYEMRIHKRY IDLEAPVQIV KRITQITIEP GVDVEVVVAS 


N

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References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Suppression of yeast RNA polymerase III mutations by the URP2 gene encoding a protein homologous to the mammalian ribosomal protein S20." Hermann-Le Denmat S., Sipickzki M., Thuriaux P. J. Mol. Biol. 240:1-7(1994) [PubMed: 8021936] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 28383 / FL100 / VTT C-80102.
[2]	"Complete nucleotide sequence of Saccharomyces cerevisiae chromosome VIII." Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Dover J., Du Z., Favello A., Fulton L., Gattung S., Geisel C., Kirsten J., Kucaba T., Hillier L.W., Jier M., Johnston L., Langston Y., Latreille P. Vaudin M. Science 265:2077-2082(1994) [PubMed: 8091229] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	Bienvenut W.V., Peters C. Submitted (MAY-2005) to UniProtKB Cited for: PROTEIN SEQUENCE OF 2-21; 33-43; 78-85 AND 90-102, CLEAVAGE OF INITIATOR METHIONINE, ACETYLATION AT SER-2, MASS SPECTROMETRY.
[5]	"A proteomics approach to understanding protein ubiquitination." Peng J., Schwartz D., Elias J.E., Thoreen C.C., Cheng D., Marsischky G., Roelofs J., Finley D., Gygi S.P. Nat. Biotechnol. 21:921-926(2003) [PubMed: 12872131] [Abstract] Cited for: PROTEIN SEQUENCE OF 7-21, MASS SPECTROMETRY, UBIQUITINATION [LARGE SCALE ANALYSIS] AT LYS-8. Strain: SUB592.
[6]	"The list of cytoplasmic ribosomal proteins of Saccharomyces cerevisiae." Planta R.J., Mager W.H. Yeast 14:471-477(1998) [PubMed: 9559554] [Abstract] Cited for: NOMENCLATURE, SUBUNIT.
[7]	"The action of N-terminal acetyltransferases on yeast ribosomal proteins." Arnold R.J., Polevoda B., Reilly J.P., Sherman F. J. Biol. Chem. 274:37035-37040(1999) [PubMed: 10601260] [Abstract] Cited for: CLEAVAGE OF INITIATOR METHIONINE, ACETYLATION AT SER-2 BY NATA.
[8]	"A subset of membrane-associated proteins is ubiquitinated in response to mutations in the endoplasmic reticulum degradation machinery." Hitchcock A.L., Auld K., Gygi S.P., Silver P.A. Proc. Natl. Acad. Sci. U.S.A. 100:12735-12740(2003) [PubMed: 14557538] [Abstract] Cited for: UBIQUITINATION [LARGE SCALE ANALYSIS] AT LYS-8, MASS SPECTROMETRY.
[9]	"Quantitative phosphoproteomics applied to the yeast pheromone signaling pathway." Gruhler A., Olsen J.V., Mohammed S., Mortensen P., Faergeman N.J., Mann M., Jensen O.N. Mol. Cell. Proteomics 4:310-327(2005) [PubMed: 15665377] [Abstract] Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT SER-2, PHOSPHORYLATION AT SER-2, MASS SPECTROMETRY. Strain: YAL6B.
[10]	"Structure of the 80S ribosome from Saccharomyces cerevisiae -- tRNA-ribosome and subunit-subunit interactions." Spahn C.M.T., Beckmann R., Eswar N., Penczek P.A., Sali A., Blobel G., Frank J. Cell 107:373-386(2001) [PubMed: 11701127] [Abstract] Cited for: 3D-STRUCTURE MODELING OF 21-117, ELECTRON MICROSCOPY.
[11]	"Domain movements of elongation factor eEF2 and the eukaryotic 80S ribosome facilitate tRNA translocation." Spahn C.M.T., Gomez-Lorenzo M.G., Grassucci R.A., Joergensen R., Andersen G.R., Beckmann R., Penczek P.A., Ballesta J.P.G., Frank J. EMBO J. 23:1008-1019(2004) [PubMed: 14976550] [Abstract] Cited for: 3D-STRUCTURE MODELING OF 21-121, ELECTRON MICROSCOPY.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

Z29089 Genomic DNA. Translation: CAA82331.1.
U11582 Genomic DNA. Translation: AAB65068.1.
BK006934 Genomic DNA. Translation: DAA06671.1.

PIR

S46829.

RefSeq

NP_011848.1. NM_001179095.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1K5X	model	-	J	21-117	[»]
1S1H	electron microscopy	11.70	J	21-117	[»]
3IZB	electron microscopy	-	J	1-121	[»]
3O2Z	X-ray	4.00	N	1-121	[»]
3O30	X-ray	4.00	N	1-121	[»]

ProteinModelPortal

P38701.

SMR

P38701. Positions 21-116.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-4060N.

IntAct

P38701. 6 interactions.

MINT

MINT-539826.

STRING

P38701.

Proteomic databases

PeptideAtlas

P38701.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblFungi

YHL015W; YHL015W; YHL015W.

GeneID

856371.

KEGG

sce:YHL015W.

NMPDR

fig|4932.3.peg.2988.

Organism-specific databases

SGD

S000001007. RPS20.

Phylogenomic databases

eggNOG

fuNOG10065.

GeneTree

EFGT00050000003241.

HOGENOM

HBG317444.

OMA

YEMRIHK.

OrthoDB

EOG4SFDGK.

Gene expression databases

ArrayExpress

P38701.

Genevestigator

P38701.

GermOnline

YHL015W. Saccharomyces cerevisiae.

Family and domain databases

InterPro

IPR001848. Ribosomal_S10.
IPR018268. Ribosomal_S10_CS.
IPR005729. Ribosomal_S10_euk/arc.
[Graphical view]

Gene3D

G3DSA:3.30.70.600. Ribosomal_S10. 1 hit.

K02969.

PANTHER

PTHR11700. Ribosomal_S10. 1 hit.

Pfam

PF00338. Ribosomal_S10. 1 hit.
[Graphical view]

PRINTS

PR00971. RIBOSOMALS10.

SUPFAM

SSF54999. Ribosomal_S10. 1 hit.

TIGRFAMs

TIGR01046. S10_Arc_S20_Euk. 1 hit.

PROSITE

PS00361. RIBOSOMAL_S10. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

NextBio

981849.

Entry information

Entry name

RS20_YEAST

Accession

Primary (citable) accession number: P38701
Secondary accession number(s): D3DKP7

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	January 23, 2007
Last modified:	December 14, 2011
This is version 110 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program