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P38701 (RS20_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 127. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (5) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
40S ribosomal protein S20
Gene names
Name:RPS20
Synonyms:URP2
Ordered Locus Names:YHL015W
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length121 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Subunit structure

Component of the small ribosomal subunit. Mature ribosomes consist of a small (40S) and a large (60S) subunit. The 40S subunit contains 32 different proteins (encoded by 56 genes) and 1 molecule of RNA (18S). The 60S subunit contains 46 different proteins (encoded by 81 genes) and 3 molecules of RNA (25S, 5.8S and 5S). Ref.5

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00508.

Post-translational modification

N-terminally acetylated by acetyltransferase NatA. Also partially acetylated by NatC. Ref.4 Ref.6

Sequence similarities

Belongs to the ribosomal protein S10P family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed Ref.4 Ref.6
Chain2 – 12112040S ribosomal protein S20 HAMAP-Rule MF_00508
PRO_0000146692

Amino acid modifications

Modified residue21N-acetylserine Ref.4 Ref.6 Ref.9
Cross-link8Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) Ref.7 Ref.8

Secondary structure

............. 121
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P38701 [UniParc].

Last modified January 23, 2007. Version 3.
Checksum: 8B1607EDB63017F1

FASTA12113,907
        10         20         30         40         50         60 
MSDFQKEKVE EQEQQQQQII KIRITLTSTK VKQLENVSSN IVKNAEQHNL VKKGPVRLPT 

        70         80         90        100        110        120 
KVLKISTRKT PNGEGSKTWE TYEMRIHKRY IDLEAPVQIV KRITQITIEP GVDVEVVVAS 


N 

« Hide

References

« Hide 'large scale' references
[1]"Suppression of yeast RNA polymerase III mutations by the URP2 gene encoding a protein homologous to the mammalian ribosomal protein S20."
Hermann-Le Denmat S., Sipickzki M., Thuriaux P.
J. Mol. Biol. 240:1-7(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 28383 / FL100 / VTT C-80102.
[2]"Complete nucleotide sequence of Saccharomyces cerevisiae chromosome VIII."
Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Dover J., Du Z., Favello A., Fulton L., Gattung S., Geisel C., Kirsten J., Kucaba T., Hillier L.W., Jier M., Johnston L., Langston Y., Latreille P. expand/collapse author list , Louis E.J., Macri C., Mardis E., Menezes S., Mouser L., Nhan M., Rifkin L., Riles L., St Peter H., Trevaskis E., Vaughan K., Vignati D., Wilcox L., Wohldman P., Waterston R., Wilson R., Vaudin M.
Science 265:2077-2082(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[4]Bienvenut W.V., Peters C.
Submitted (MAY-2005) to UniProtKB
Cited for: PROTEIN SEQUENCE OF 2-21; 33-43; 78-85 AND 90-102, CLEAVAGE OF INITIATOR METHIONINE, ACETYLATION AT SER-2, IDENTIFICATION BY MASS SPECTROMETRY.
[5]"The list of cytoplasmic ribosomal proteins of Saccharomyces cerevisiae."
Planta R.J., Mager W.H.
Yeast 14:471-477(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NOMENCLATURE, SUBUNIT.
[6]"The action of N-terminal acetyltransferases on yeast ribosomal proteins."
Arnold R.J., Polevoda B., Reilly J.P., Sherman F.
J. Biol. Chem. 274:37035-37040(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: CLEAVAGE OF INITIATOR METHIONINE, ACETYLATION AT SER-2 BY NATA.
[7]"A proteomics approach to understanding protein ubiquitination."
Peng J., Schwartz D., Elias J.E., Thoreen C.C., Cheng D., Marsischky G., Roelofs J., Finley D., Gygi S.P.
Nat. Biotechnol. 21:921-926(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: UBIQUITINATION [LARGE SCALE ANALYSIS] AT LYS-8.
Strain: SUB592.
[8]"A subset of membrane-associated proteins is ubiquitinated in response to mutations in the endoplasmic reticulum degradation machinery."
Hitchcock A.L., Auld K., Gygi S.P., Silver P.A.
Proc. Natl. Acad. Sci. U.S.A. 100:12735-12740(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: UBIQUITINATION [LARGE SCALE ANALYSIS] AT LYS-8.
[9]"N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB."
Van Damme P., Lasa M., Polevoda B., Gazquez C., Elosegui-Artola A., Kim D.S., De Juan-Pardo E., Demeyer K., Hole K., Larrea E., Timmerman E., Prieto J., Arnesen T., Sherman F., Gevaert K., Aldabe R.
Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT SER-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[10]"Sites of ubiquitin attachment in Saccharomyces cerevisiae."
Starita L.M., Lo R.S., Eng J.K., von Haller P.D., Fields S.
Proteomics 12:236-240(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[11]"Structure of the 80S ribosome from Saccharomyces cerevisiae -- tRNA-ribosome and subunit-subunit interactions."
Spahn C.M.T., Beckmann R., Eswar N., Penczek P.A., Sali A., Blobel G., Frank J.
Cell 107:373-386(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: 3D-STRUCTURE MODELING OF 21-117, ELECTRON MICROSCOPY.
[12]"Domain movements of elongation factor eEF2 and the eukaryotic 80S ribosome facilitate tRNA translocation."
Spahn C.M.T., Gomez-Lorenzo M.G., Grassucci R.A., Joergensen R., Andersen G.R., Beckmann R., Penczek P.A., Ballesta J.P.G., Frank J.
EMBO J. 23:1008-1019(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: 3D-STRUCTURE MODELING OF 21-121, ELECTRON MICROSCOPY.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Z29089 Genomic DNA. Translation: CAA82331.1.
U11582 Genomic DNA. Translation: AAB65068.1.
BK006934 Genomic DNA. Translation: DAA06671.1.
PIRS46829.
RefSeqNP_011848.1. NM_001179095.1.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1K5Xmodel-J21-117[»]
1S1Helectron microscopy11.70J21-117[»]
3IZBelectron microscopy-J1-121[»]
3O2ZX-ray4.00N1-121[»]
3O30X-ray4.00N1-121[»]
3U5CX-ray3.00U1-121[»]
3U5GX-ray3.00U1-121[»]
4BYLelectron microscopy4.30U1-121[»]
4BYTelectron microscopy6.60U1-121[»]
ProteinModelPortalP38701.
SMRP38701. Positions 25-121.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid36408. 61 interactions.
DIPDIP-4060N.
IntActP38701. 10 interactions.
MINTMINT-539826.
STRING4932.YHL015W.

Proteomic databases

PaxDbP38701.
PeptideAtlasP38701.
PRIDEP38701.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYHL015W; YHL015W; YHL015W.
GeneID856371.
KEGGsce:YHL015W.

Organism-specific databases

SGDS000001007. RPS20.

Phylogenomic databases

eggNOGCOG0051.
GeneTreeENSGT00390000003248.
HOGENOMHOG000270245.
KOK02969.
OMAYELKIHK.
OrthoDBEOG751NTH.

Enzyme and pathway databases

BioCycYEAST:G3O-31035-MONOMER.

Gene expression databases

GenevestigatorP38701.

Family and domain databases

Gene3D3.30.70.600. 1 hit.
HAMAPMF_00508. Ribosomal_S10.
InterProIPR001848. Ribosomal_S10.
IPR018268. Ribosomal_S10_CS.
IPR027486. Ribosomal_S10_dom.
IPR005729. Ribosomal_S10_euk/arc.
[Graphical view]
PANTHERPTHR11700. PTHR11700. 1 hit.
PfamPF00338. Ribosomal_S10. 1 hit.
[Graphical view]
PRINTSPR00971. RIBOSOMALS10.
SUPFAMSSF54999. SSF54999. 1 hit.
TIGRFAMsTIGR01046. S10_Arc_S20_Euk. 1 hit.
PROSITEPS00361. RIBOSOMAL_S10. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceP38701.
NextBio981849.
PROP38701.

Entry information

Entry nameRS20_YEAST
AccessionPrimary (citable) accession number: P38701
Secondary accession number(s): D3DKP7
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: January 23, 2007
Last modified: April 16, 2014
This is version 127 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome VIII

Yeast (Saccharomyces cerevisiae) chromosome VIII: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families

Ribosomal proteins

Ribosomal proteins families and list of entries

PDB cross-references

Index of Protein Data Bank (PDB) cross-references