P38700 (APM2_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Adaptin medium chain homolog APM2

Alternative name(s):
Adaptin-mu1-II

Gene names

Name:	APM2
Ordered Locus Names:	YHL019C

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	605 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Subunit structure	Interacts with APL2, probably forming an alternative AP-1-like complex. Ref.5
Subcellular location	Membrane › coated pit.
Miscellaneous	Present with 2890 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the adaptor complexes medium subunit family. Contains 1 MHD (mu homology) domain.

Ontologies

Keywords
Cellular component	Coated pit Membrane
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	Golgi to vacuole transport Inferred from mutant phenotype PubMed 17003107. Source: SGD intracellular protein transport Inferred from electronic annotation. Source: InterPro
Cellular_component	AP-1 adaptor complex Inferred from physical interaction Ref.5. Source: SGD coated pit Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	clathrin binding Inferred from sequence or structural similarity Ref.5. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
APL2	P36000	7	EBI-2705,EBI-2206

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 605	605	Adaptin medium chain homolog APM2		PRO_0000193793
Regions
	Domain	266 – 605	340	MHD

Sequences

	Sequence		Length	Mass (Da)
	P38700 [UniParc]. Last modified February 1, 1995. Version 1. Checksum: 7E216B11325EEE3C	FASTA	605	69,990
	10 20 30 40 50 60 MSSSLFILDE NLEPLVSKNI RALPNLSSVL SSFKQCYHDG SPPILSQNDW FFIHLKRDFL 70 80 90 100 110 120 HFVSVIHTTD KPNIDLMTIL AFLEQFYHLL QKYFEIEVLT KNVILDNILL VLELIDECID 130 140 150 160 170 180 FGIVQVTDPS IIKDYIRVKV NVPRVTVDNE EWSPGEESSS SSGSDSDSEY SNTNKRKDKK 190 200 210 220 230 240 KKRKKKKGTK GKSVGKSKLK SIMVNNKENR GINVVETVKE TLRNKNDTGK EAANDELPND 250 260 270 280 290 300 GNDLYINGDI AKTIIMPISW RTKGIHYAKN EFFLDVIERV QYLMDFEKGV IRKNLIHGEI 310 320 330 340 350 360 VCRCYLSGMP KLKISINKIL NRDPQFMSNS SFHQCVSLDS INTIEKDEEK NSDDDAGLQA 370 380 390 400 410 420 ATDAREIEFI PPDGEFVLCQ YELKRHVKDA PMVRLKDFEI KPKLKKFKIQ IVTKIQTNFK 430 440 450 460 470 480 PTNSTSKLNV RIPLTKVFQE YKIDLSKQIR FKANIGKVVF NLSDDFLLWE IQTMKGHREH 490 500 510 520 530 540 STNKSSQYNS DEDDPNTCAS MVAEFPLFNQ EEYDRLQEEM KTSMNPPPLR TGPRLEELYR 550 560 570 580 590 600 QVHDQQTSHV TPRDKLVNID FEIPYCTCSG LKVEYLKVEE PQLQYQSFPW VRYKTVSDEE YAYIV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A late Golgi sorting function for Saccharomyces cerevisiae Apm1p, but not for Apm2p, a second yeast clathrin AP medium chain-related protein." Stepp J.D., Pellicena-Palle A., Hamilton S., Kirchhausen T., Lemmon S.K. Mol. Biol. Cell 6:41-58(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: S288c / REE526.
[2]	"Complete nucleotide sequence of Saccharomyces cerevisiae chromosome VIII." Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Dover J., Du Z., Favello A., Fulton L., Gattung S., Geisel C., Kirsten J., Kucaba T., Hillier L.W., Jier M., Johnston L., Langston Y., Latreille P. , Louis E.J., Macri C., Mardis E., Menezes S., Mouser L., Nhan M., Rifkin L., Riles L., St Peter H., Trevaskis E., Vaughan K., Vignati D., Wilcox L., Wohldman P., Waterston R., Wilson R., Vaudin M. Science 265:2077-2082(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[5]	"Adaptor complex-independent clathrin function in yeast." Yeung B.G., Phan H.L., Payne G.S. Mol. Biol. Cell 10:3643-3659(1999) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH APL2.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U09841 Genomic DNA. Translation: AAA83415.1. U11582 Genomic DNA. Translation: AAB65072.1. BK006934 Genomic DNA. Translation: DAA06666.1.
PIR	S46833.
RefSeq	NP_011844.1. NM_001179099.1.
3D structure databases
ProteinModelPortal	P38700.
SMR	P38700. Positions 258-593.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-1200N.
IntAct	P38700. 9 interactions.
MINT	MINT-404347.
STRING	4932.YHL019C.
Proteomic databases
PaxDb	P38700.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YHL019C; YHL019C; YHL019C.
GeneID	856367.
KEGG	sce:YHL019C.
Organism-specific databases
CYGD	YHL019c.
SGD	S000001011. APM2.
Phylogenomic databases
eggNOG	NOG292341.
HOGENOM	HOG000033997.
OMA	KNEFFLD.
OrthoDB	EOG4NCQNH.
Gene expression databases
Genevestigator	P38700.
GermOnline	YHL019C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR027200. Apm2. IPR008968. Clathrin_mu_C. IPR018240. Clathrin_mu_CS. IPR011012. Longin-like_dom. [Graphical view]
PANTHER	PTHR11998:SF5. PTHR11998:SF5. 1 hit.
Pfam	PF00928. Adap_comp_sub. 1 hit. [Graphical view]
SUPFAM	SSF49447. AP50. 1 hit. SSF64356. Longin_like. 1 hit.
PROSITE	PS00990. CLAT_ADAPTOR_M_1. 1 hit. PS00991. CLAT_ADAPTOR_M_2. 1 hit. PS51072. MHD. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	981837.

Entry information

Entry name

APM2_YEAST

Accession

Primary (citable) accession number: P38700
Secondary accession number(s): D3DKR4

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	February 1, 1995
Last modified:	May 1, 2013
This is version 101 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program