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Protein

Exopolyphosphatase

Gene

PPX1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Degradation of inorganic polyphosphates.

Catalytic activityi

(Polyphosphate)(n) + H2O = (polyphosphate)(n-1) + phosphate.

Cofactori

Mn2+CuratedNote: Binds 2 manganese ions per subunit.Curated

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi39 – 391Manganese 1By similarity
Metal bindingi41 – 411Manganese 2By similarity
Metal bindingi127 – 1271Manganese 1By similarity
Metal bindingi127 – 1271Manganese 2By similarity
Metal bindingi148 – 1481Manganese 2By similarity
Metal bindingi202 – 2021Manganese 2By similarity

GO - Molecular functioni

  • exopolyphosphatase activity Source: SGD
  • metal ion binding Source: UniProtKB-KW

GO - Biological processi

  • polyphosphate catabolic process Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

Manganese, Metal-binding

Enzyme and pathway databases

BioCyciYEAST:YHR201C-MONOMER.
BRENDAi3.6.1.11. 984.

Names & Taxonomyi

Protein namesi
Recommended name:
Exopolyphosphatase (EC:3.6.1.11)
Short name:
ExopolyPase
Alternative name(s):
Metaphosphatase
Gene namesi
Name:PPX1
Ordered Locus Names:YHR201C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311 Componenti: Chromosome VIII

Organism-specific databases

CYGDiYHR201c.
EuPathDBiFungiDB:YHR201C.
SGDiS000001244. PPX1.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: SGD
  • cytosol Source: SGD
  • mitochondrial matrix Source: SGD
  • plasma membrane Source: SGD
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 397397ExopolyphosphatasePRO_0000158601Add
BLAST

Proteomic databases

MaxQBiP38698.
PaxDbiP38698.
PeptideAtlasiP38698.
PRIDEiP38698.

Interactioni

Protein-protein interaction databases

BioGridi36635. 14 interactions.
DIPiDIP-5633N.
IntActiP38698. 2 interactions.
MINTiMINT-540566.

Structurei

Secondary structure

1
397
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi8 – 1710Combined sources
Helixi20 – 234Combined sources
Beta strandi28 – 336Combined sources
Helixi40 – 5819Combined sources
Helixi60 – 623Combined sources
Beta strandi63 – 653Combined sources
Beta strandi72 – 743Combined sources
Helixi79 – 846Combined sources
Helixi86 – 949Combined sources
Helixi99 – 1013Combined sources
Helixi105 – 11410Combined sources
Beta strandi120 – 1278Combined sources
Helixi133 – 1353Combined sources
Turni136 – 1383Combined sources
Beta strandi141 – 1477Combined sources
Beta strandi159 – 1635Combined sources
Helixi169 – 18012Combined sources
Turni181 – 1833Combined sources
Helixi185 – 20218Combined sources
Turni203 – 2075Combined sources
Helixi212 – 22615Combined sources
Helixi235 – 25117Combined sources
Helixi259 – 2646Combined sources
Beta strandi267 – 2726Combined sources
Beta strandi275 – 2784Combined sources
Beta strandi280 – 28910Combined sources
Helixi291 – 2977Combined sources
Helixi301 – 31414Combined sources
Beta strandi318 – 32811Combined sources
Beta strandi331 – 34010Combined sources
Helixi342 – 35615Combined sources
Beta strandi358 – 3647Combined sources
Helixi365 – 3673Combined sources
Beta strandi369 – 3746Combined sources
Helixi381 – 39313Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2QB6X-ray1.80A/B1-397[»]
2QB7X-ray1.60A/B1-397[»]
2QB8X-ray1.90A/B1-397[»]
ProteinModelPortaliP38698.
SMRiP38698. Positions 5-397.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP38698.

Family & Domainsi

Sequence similaritiesi

Belongs to the PPase class C family.Curated

Phylogenomic databases

eggNOGiCOG1227.
GeneTreeiENSGT00450000040262.
HOGENOMiHOG000248436.
InParanoidiP38698.
KOiK01514.
OMAiDTVNFEP.
OrthoDBiEOG7FNCH9.

Family and domain databases

InterProiIPR001667. DDH_dom.
IPR004097. DHHA2.
[Graphical view]
PfamiPF01368. DHH. 1 hit.
PF02833. DHHA2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P38698-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSPLRKTVPE FLAHLKSLPI SKIASNDVLT ICVGNESADM DSIASAITYS
60 70 80 90 100
YCQYIYNEGT YSEEKKKGSF IVPIIDIPRE DLSLRRDVMY VLEKLKIKEE
110 120 130 140 150
ELFFIEDLKS LKQNVSQGTE LNSYLVDNND TPKNLKNYID NVVGIIDHHF
160 170 180 190 200
DLQKHLDAEP RIVKVSGSCS SLVFNYWYEK LQGDREVVMN IAPLLMGAIL
210 220 230 240 250
IDTSNMRRKV EESDKLAIER CQAVLSGAVN EVSAQGLEDS SEFYKEIKSR
260 270 280 290 300
KNDIKGFSVS DILKKDYKQF NFQGKGHKGL EIGLSSIVKR MSWLFNEHGG
310 320 330 340 350
EADFVNQCRR FQAERGLDVL VLLTSWRKAG DSHRELVILG DSNVVRELIE
360 370 380 390
RVSDKLQLQL FGGNLDGGVA MFKQLNVEAT RKQVVPYLEE AYSNLEE
Length:397
Mass (Da):45,051
Last modified:February 1, 1995 - v1
Checksum:i9494CB47790E07B8
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti193 – 1931P → L in AAA65933 (PubMed:7860598).Curated
Sequence conflicti284 – 2841L → F in AAA65933 (PubMed:7860598).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L28711 Genomic DNA. Translation: AAA65933.1.
U00030 Genomic DNA. Translation: AAB68368.1.
BK006934 Genomic DNA. Translation: DAA06894.1.
PIRiS46691.
RefSeqiNP_012071.1. NM_001179332.1.

Genome annotation databases

EnsemblFungiiYHR201C; YHR201C; YHR201C.
GeneIDi856608.
KEGGisce:YHR201C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L28711 Genomic DNA. Translation: AAA65933.1.
U00030 Genomic DNA. Translation: AAB68368.1.
BK006934 Genomic DNA. Translation: DAA06894.1.
PIRiS46691.
RefSeqiNP_012071.1. NM_001179332.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2QB6X-ray1.80A/B1-397[»]
2QB7X-ray1.60A/B1-397[»]
2QB8X-ray1.90A/B1-397[»]
ProteinModelPortaliP38698.
SMRiP38698. Positions 5-397.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi36635. 14 interactions.
DIPiDIP-5633N.
IntActiP38698. 2 interactions.
MINTiMINT-540566.

Proteomic databases

MaxQBiP38698.
PaxDbiP38698.
PeptideAtlasiP38698.
PRIDEiP38698.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYHR201C; YHR201C; YHR201C.
GeneIDi856608.
KEGGisce:YHR201C.

Organism-specific databases

CYGDiYHR201c.
EuPathDBiFungiDB:YHR201C.
SGDiS000001244. PPX1.

Phylogenomic databases

eggNOGiCOG1227.
GeneTreeiENSGT00450000040262.
HOGENOMiHOG000248436.
InParanoidiP38698.
KOiK01514.
OMAiDTVNFEP.
OrthoDBiEOG7FNCH9.

Enzyme and pathway databases

BioCyciYEAST:YHR201C-MONOMER.
BRENDAi3.6.1.11. 984.

Miscellaneous databases

EvolutionaryTraceiP38698.
NextBioi982520.
PROiP38698.

Family and domain databases

InterProiIPR001667. DDH_dom.
IPR004097. DHHA2.
[Graphical view]
PfamiPF01368. DHH. 1 hit.
PF02833. DHHA2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The gene for a major exopolyphosphatase of Saccharomyces cerevisiae."
    Wurst H., Shiba T., Kornberg A.
    J. Bacteriol. 177:898-906(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: S288c / SNY243.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiPPX1_YEAST
AccessioniPrimary (citable) accession number: P38698
Secondary accession number(s): D3DLF0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: July 22, 2015
This is version 112 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 3170 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome VIII
    Yeast (Saccharomyces cerevisiae) chromosome VIII: entries and gene names

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.