Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

P38698

- PPX1_YEAST

UniProt

P38698 - PPX1_YEAST

Protein

Exopolyphosphatase

Gene

PPX1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 106 (01 Oct 2014)
      Sequence version 1 (01 Feb 1995)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Degradation of inorganic polyphosphates.

    Catalytic activityi

    (Polyphosphate)(n) + H2O = (polyphosphate)(n-1) + phosphate.

    Cofactori

    Binds 2 manganese ions per subunit.Curated

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi39 – 391Manganese 1By similarity
    Metal bindingi41 – 411Manganese 2By similarity
    Metal bindingi127 – 1271Manganese 1By similarity
    Metal bindingi127 – 1271Manganese 2By similarity
    Metal bindingi148 – 1481Manganese 2By similarity
    Metal bindingi202 – 2021Manganese 2By similarity

    GO - Molecular functioni

    1. exopolyphosphatase activity Source: SGD
    2. manganese ion binding Source: InterPro

    GO - Biological processi

    1. polyphosphate catabolic process Source: SGD

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Ligandi

    Manganese, Metal-binding

    Enzyme and pathway databases

    BioCyciYEAST:YHR201C-MONOMER.
    BRENDAi3.6.1.11. 984.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Exopolyphosphatase (EC:3.6.1.11)
    Short name:
    ExopolyPase
    Alternative name(s):
    Metaphosphatase
    Gene namesi
    Name:PPX1
    Ordered Locus Names:YHR201C
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome VIII

    Organism-specific databases

    CYGDiYHR201c.
    SGDiS000001244. PPX1.

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: SGD
    2. cytosol Source: SGD
    3. mitochondrial matrix Source: SGD
    4. plasma membrane Source: SGD

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 397397ExopolyphosphatasePRO_0000158601Add
    BLAST

    Proteomic databases

    MaxQBiP38698.
    PaxDbiP38698.
    PeptideAtlasiP38698.
    PRIDEiP38698.

    Expressioni

    Gene expression databases

    GenevestigatoriP38698.

    Interactioni

    Protein-protein interaction databases

    BioGridi36635. 13 interactions.
    DIPiDIP-5633N.
    IntActiP38698. 2 interactions.
    MINTiMINT-540566.
    STRINGi4932.YHR201C.

    Structurei

    Secondary structure

    1
    397
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi8 – 1710
    Helixi20 – 234
    Beta strandi28 – 336
    Helixi40 – 5819
    Helixi60 – 623
    Beta strandi63 – 653
    Beta strandi72 – 743
    Helixi79 – 846
    Helixi86 – 949
    Helixi99 – 1013
    Helixi105 – 11410
    Beta strandi120 – 1278
    Helixi133 – 1353
    Turni136 – 1383
    Beta strandi141 – 1477
    Beta strandi159 – 1635
    Helixi169 – 18012
    Turni181 – 1833
    Helixi185 – 20218
    Turni203 – 2075
    Helixi212 – 22615
    Helixi235 – 25117
    Helixi259 – 2646
    Beta strandi267 – 2726
    Beta strandi275 – 2784
    Beta strandi280 – 28910
    Helixi291 – 2977
    Helixi301 – 31414
    Beta strandi318 – 32811
    Beta strandi331 – 34010
    Helixi342 – 35615
    Beta strandi358 – 3647
    Helixi365 – 3673
    Beta strandi369 – 3746
    Helixi381 – 39313

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2QB6X-ray1.80A/B1-397[»]
    2QB7X-ray1.60A/B1-397[»]
    2QB8X-ray1.90A/B1-397[»]
    ProteinModelPortaliP38698.
    SMRiP38698. Positions 5-397.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP38698.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the PPase class C family.Curated

    Phylogenomic databases

    eggNOGiCOG1227.
    GeneTreeiENSGT00450000040262.
    HOGENOMiHOG000248436.
    KOiK01514.
    OMAiDTVNFEP.
    OrthoDBiEOG7FNCH9.

    Family and domain databases

    InterProiIPR001667. DDH_dom.
    IPR004097. DHHA2.
    [Graphical view]
    PfamiPF01368. DHH. 1 hit.
    PF02833. DHHA2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P38698-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSPLRKTVPE FLAHLKSLPI SKIASNDVLT ICVGNESADM DSIASAITYS    50
    YCQYIYNEGT YSEEKKKGSF IVPIIDIPRE DLSLRRDVMY VLEKLKIKEE 100
    ELFFIEDLKS LKQNVSQGTE LNSYLVDNND TPKNLKNYID NVVGIIDHHF 150
    DLQKHLDAEP RIVKVSGSCS SLVFNYWYEK LQGDREVVMN IAPLLMGAIL 200
    IDTSNMRRKV EESDKLAIER CQAVLSGAVN EVSAQGLEDS SEFYKEIKSR 250
    KNDIKGFSVS DILKKDYKQF NFQGKGHKGL EIGLSSIVKR MSWLFNEHGG 300
    EADFVNQCRR FQAERGLDVL VLLTSWRKAG DSHRELVILG DSNVVRELIE 350
    RVSDKLQLQL FGGNLDGGVA MFKQLNVEAT RKQVVPYLEE AYSNLEE 397
    Length:397
    Mass (Da):45,051
    Last modified:February 1, 1995 - v1
    Checksum:i9494CB47790E07B8
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti193 – 1931P → L in AAA65933. (PubMed:7860598)Curated
    Sequence conflicti284 – 2841L → F in AAA65933. (PubMed:7860598)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L28711 Genomic DNA. Translation: AAA65933.1.
    U00030 Genomic DNA. Translation: AAB68368.1.
    BK006934 Genomic DNA. Translation: DAA06894.1.
    PIRiS46691.
    RefSeqiNP_012071.1. NM_001179332.1.

    Genome annotation databases

    EnsemblFungiiYHR201C; YHR201C; YHR201C.
    GeneIDi856608.
    KEGGisce:YHR201C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L28711 Genomic DNA. Translation: AAA65933.1 .
    U00030 Genomic DNA. Translation: AAB68368.1 .
    BK006934 Genomic DNA. Translation: DAA06894.1 .
    PIRi S46691.
    RefSeqi NP_012071.1. NM_001179332.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2QB6 X-ray 1.80 A/B 1-397 [» ]
    2QB7 X-ray 1.60 A/B 1-397 [» ]
    2QB8 X-ray 1.90 A/B 1-397 [» ]
    ProteinModelPortali P38698.
    SMRi P38698. Positions 5-397.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 36635. 13 interactions.
    DIPi DIP-5633N.
    IntActi P38698. 2 interactions.
    MINTi MINT-540566.
    STRINGi 4932.YHR201C.

    Proteomic databases

    MaxQBi P38698.
    PaxDbi P38698.
    PeptideAtlasi P38698.
    PRIDEi P38698.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YHR201C ; YHR201C ; YHR201C .
    GeneIDi 856608.
    KEGGi sce:YHR201C.

    Organism-specific databases

    CYGDi YHR201c.
    SGDi S000001244. PPX1.

    Phylogenomic databases

    eggNOGi COG1227.
    GeneTreei ENSGT00450000040262.
    HOGENOMi HOG000248436.
    KOi K01514.
    OMAi DTVNFEP.
    OrthoDBi EOG7FNCH9.

    Enzyme and pathway databases

    BioCyci YEAST:YHR201C-MONOMER.
    BRENDAi 3.6.1.11. 984.

    Miscellaneous databases

    EvolutionaryTracei P38698.
    NextBioi 982520.
    PROi P38698.

    Gene expression databases

    Genevestigatori P38698.

    Family and domain databases

    InterProi IPR001667. DDH_dom.
    IPR004097. DHHA2.
    [Graphical view ]
    Pfami PF01368. DHH. 1 hit.
    PF02833. DHHA2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The gene for a major exopolyphosphatase of Saccharomyces cerevisiae."
      Wurst H., Shiba T., Kornberg A.
      J. Bacteriol. 177:898-906(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: S288c / SNY243.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    4. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiPPX1_YEAST
    AccessioniPrimary (citable) accession number: P38698
    Secondary accession number(s): D3DLF0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1995
    Last sequence update: February 1, 1995
    Last modified: October 1, 2014
    This is version 106 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 3170 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families
    3. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    4. Yeast chromosome VIII
      Yeast (Saccharomyces cerevisiae) chromosome VIII: entries and gene names

    External Data

    Dasty 3