Reviewed, UniProtKB/Swiss-Prot P38698 (PPX1_YEAST)

Names and origin

Protein names

Recommended name:
    Exopolyphosphatase
      Short name=ExopolyPase
    EC=3.6.1.11
Alternative name(s):
    Metaphosphatase

Gene names

Name:	PPX1
Ordered Locus Names:	YHR201C

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	397 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Degradation of inorganic polyphosphates.
Catalytic activity	(Polyphosphate)(n) + H2O = (polyphosphate)(n-1) + phosphate.
Cofactor	Binds 2 manganese ions per subunit Potential.
Miscellaneous	Present with 3170 molecules/cell in log phase SD medium. Ref.3
Sequence similarities	Belongs to the PPase class C family.

Ontologies

Keywords
Ligand	Manganese Metal-binding
Molecular function	Hydrolase
Technical term	3D-structure Complete proteome
Gene Ontology (GO)
Biological process	polyphosphate catabolic process Ref.1 Inferred from direct assay. Source: SGD
Cellular component	cytosol Traceable author statement. Source: SGD mitochondrial matrix Traceable author statement. Source: SGD plasma membrane Traceable author statement. Source: SGD
Molecular function	exopolyphosphatase activity Ref.1 Inferred from direct assay. Source: SGD manganese ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 397	397	Exopolyphosphatase		PRO_0000158601
Sites
	Metal binding	39	1	Manganese 1 By similarity
	Metal binding	41	1	Manganese 2 By similarity
	Metal binding	127	1	Manganese 1 By similarity
	Metal binding	127	1	Manganese 2 By similarity
	Metal binding	148	1	Manganese 2 By similarity
	Metal binding	202	1	Manganese 2 By similarity
Experimental info
	Sequence conflict	193	1	P → L in AAA65933. Ref.1
	Sequence conflict	284	1	L → F in AAA65933. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P38698-1 [UniParc]. Last modified February 1, 1995. Version 1. Checksum: 9494CB47790E07B8	FASTA	397	45,051
	10 20 30 40 50 60 MSPLRKTVPE FLAHLKSLPI SKIASNDVLT ICVGNESADM DSIASAITYS YCQYIYNEGT 70 80 90 100 110 120 YSEEKKKGSF IVPIIDIPRE DLSLRRDVMY VLEKLKIKEE ELFFIEDLKS LKQNVSQGTE 130 140 150 160 170 180 LNSYLVDNND TPKNLKNYID NVVGIIDHHF DLQKHLDAEP RIVKVSGSCS SLVFNYWYEK 190 200 210 220 230 240 LQGDREVVMN IAPLLMGAIL IDTSNMRRKV EESDKLAIER CQAVLSGAVN EVSAQGLEDS 250 260 270 280 290 300 SEFYKEIKSR KNDIKGFSVS DILKKDYKQF NFQGKGHKGL EIGLSSIVKR MSWLFNEHGG 310 320 330 340 350 360 EADFVNQCRR FQAERGLDVL VLLTSWRKAG DSHRELVILG DSNVVRELIE RVSDKLQLQL 370 380 390 FGGNLDGGVA MFKQLNVEAT RKQVVPYLEE AYSNLEE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The gene for a major exopolyphosphatase of Saccharomyces cerevisiae." Wurst H., Shiba T., Kornberg A. J. Bacteriol. 177:898-906(1995) [PubMed: 7860598] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: S288c / SNY243.
[2]	"Complete nucleotide sequence of Saccharomyces cerevisiae chromosome VIII." Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Dover J., Du Z., Favello A., Fulton L., Gattung S., Geisel C., Kirsten J., Kucaba T., Hillier L.W., Jier M., Johnston L., Langston Y., Latreille P. , Louis E.J., Macri C., Mardis E., Menezes S., Mouser L., Nhan M., Rifkin L., Riles L., St Peter H., Trevaskis E., Vaughan K., Vignati D., Wilcox L., Wohldman P., Waterston R., Wilson R., Vaudin M. Science 265:2077-2082(1994) [PubMed: 8091229] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[3]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBLGenBankDDBJ

L28711 Genomic DNA. Translation: AAA65933.1.
U00030 Genomic DNA. Translation: AAB68368.1.

PIR

S46691.

RefSeq

NP_012071.1.

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2QB6	X-ray	1.80	A/B	1-397	[»]
2QB7	X-ray	1.60	A/B	1-397	[»]
2QB8	X-ray	1.90	A/B	1-397	[»]

ModBase

Search...

Protein-protein interaction databases

DIP

DIP:5633N.

IntAct

P38698. 4 interactions.

Proteomic databases

PeptideAtlas

P38698.

Genome annotation databases

Ensembl

YHR201C. Saccharomyces cerevisiae. [Contig view]

GeneID

856608.

GenomeReviews

Gene locus YHR201C in contig U00093_GR.

KEGG

sce:YHR201C.

NMPDR

fig|4932.3.peg.3242.

Organism-specific databases

CYGD

YHR201c.

SGD

S000001244. PPX1.

Yeast-GFP

Search...

Phylogenomic databases

HOGENOM

P38698.

OMA

P38698. DTVNFEP.

Enzyme and pathway databases

BRENDA

3.6.1.11. 250.

Gene expression databases

ArrayExpress

P38698.

GermOnline

YHR201C. Saccharomyces cerevisiae.

Family and domain databases

InterPro

IPR004097. DHHA2.
IPR001667. Pesterase_RecJ.
[Graphical view]

Pfam

PF01368. DHH. 1 hit.
PF02833. DHHA2. 1 hit.
[Graphical view]

ProtoNet

Search...

Other Resources

NextBio

982520.

Entry information

Entry name

PPX1_YEAST

Accession

Primary (citable) accession number: P38698

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	February 1, 1995
Last modified:	June 16, 2009
This is version 64 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)