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P38697

- IMDH2_YEAST

UniProt

P38697 - IMDH2_YEAST

Protein

Inosine-5'-monophosphate dehydrogenase 2

Gene

IMD2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 136 (01 Oct 2014)
      Sequence version 1 (01 Feb 1995)
      Previous versions | rss
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    Functioni

    Catalyzes the conversion of inosine 5'-phosphate (IMP) to xanthosine 5'-phosphate (XMP), the first committed and rate-limiting step in the de novo synthesis of guanine nucleotides, and therefore plays an important role in the regulation of cell growth. In contrast to the other IMPDH alleles IMD3 and IMD4, the enzymatic activity of IMD2 seems to be intrinsically drug resistant.3 PublicationsUniRule annotation

    Catalytic activityi

    Inosine 5'-phosphate + NAD+ + H2O = xanthosine 5'-phosphate + NADH.UniRule annotation

    Cofactori

    Potassium.UniRule annotation

    Enzyme regulationi

    Mycophenolic acid (MPA) is a non-competitive inhibitor that prevents formation of the closed enzyme conformation by binding to the same site as the amobile flap. In contrast, mizoribine monophosphate (MZP) is a competitive inhibitor that induces the closed conformation. MPA is a potent inhibitor of mammalian IMPDHs but a poor inhibitor of the bacterial enzymes. MZP is a more potent inhibitor of bacterial IMPDH.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi330 – 3301Potassium; via carbonyl oxygenUniRule annotation
    Metal bindingi332 – 3321Potassium; via carbonyl oxygenUniRule annotation
    Binding sitei333 – 3331IMPUniRule annotation
    Active sitei335 – 3351Thioimidate intermediateUniRule annotation
    Metal bindingi335 – 3351Potassium; via carbonyl oxygenUniRule annotation
    Binding sitei449 – 4491IMPUniRule annotation
    Metal bindingi508 – 5081Potassium; via carbonyl oxygen; shared with tetrameric partnerUniRule annotation
    Metal bindingi509 – 5091Potassium; via carbonyl oxygen; shared with tetrameric partnerUniRule annotation
    Metal bindingi510 – 5101Potassium; via carbonyl oxygen; shared with tetrameric partnerUniRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi278 – 2803NADUniRule annotation
    Nucleotide bindingi328 – 3303NADUniRule annotation

    GO - Molecular functioni

    1. adenyl nucleotide binding Source: InterPro
    2. chromatin binding Source: SGD
    3. IMP dehydrogenase activity Source: SGD
    4. metal ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. GMP biosynthetic process Source: UniProtKB-HAMAP
    2. GTP biosynthetic process Source: SGD

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    GMP biosynthesis, Purine biosynthesis

    Keywords - Ligandi

    Metal-binding, NAD, Potassium

    Enzyme and pathway databases

    BioCyciYEAST:YHR216W-MONOMER.
    SABIO-RKP38697.
    UniPathwayiUPA00601; UER00295.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Inosine-5'-monophosphate dehydrogenase 2UniRule annotation (EC:1.1.1.205UniRule annotation)
    Short name:
    IMP dehydrogenase 2UniRule annotation
    Short name:
    IMPD 2UniRule annotation
    Short name:
    IMPDH 2UniRule annotation
    Gene namesi
    Name:IMD2UniRule annotation
    Synonyms:PUR5
    Ordered Locus Names:YHR216W
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome VIII

    Organism-specific databases

    CYGDiYHR216w.
    SGDiS000001259. IMD2.

    Subcellular locationi

    Cytoplasm 1 PublicationUniRule annotation

    GO - Cellular componenti

    1. chromatin Source: SGD
    2. cytoplasm Source: SGD

    Keywords - Cellular componenti

    Cytoplasm

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi253 – 2531S → A: Reduces drug-resistance to MPA. 1 Publication
    Mutagenesisi335 – 3351C → A: Inactivates the proteins ability to provide drug-resistance in vivo. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 523523Inosine-5'-monophosphate dehydrogenase 2PRO_0000093682Add
    BLAST

    Proteomic databases

    MaxQBiP38697.

    Expressioni

    Inductioni

    Induced by MPA resulting in resistance to the drug. Repressed by nutrient limitation.1 Publication

    Gene expression databases

    GenevestigatoriP38697.

    Interactioni

    Subunit structurei

    Homotetramer. Seems to be able to form heterotetramers composed from more than 1 of the 3 IMPDH gene products (IMD2-4).1 PublicationUniRule annotation

    Protein-protein interaction databases

    BioGridi36651. 61 interactions.
    DIPiDIP-2840N.
    IntActiP38697. 37 interactions.
    MINTiMINT-521280.
    STRINGi4932.YHR216W.

    Structurei

    3D structure databases

    ProteinModelPortaliP38697.
    SMRiP38697. Positions 2-521.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini121 – 18363CBS 1UniRule annotationAdd
    BLAST
    Domaini184 – 24057CBS 2UniRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni368 – 3703IMP bindingUniRule annotation
    Regioni391 – 3922IMP bindingUniRule annotation
    Regioni415 – 4195IMP bindingUniRule annotation

    Sequence similaritiesi

    Belongs to the IMPDH/GMPR family.UniRule annotation
    Contains 2 CBS domains.UniRule annotation

    Keywords - Domaini

    CBS domain, Repeat

    Phylogenomic databases

    GeneTreeiENSGT00530000062923.
    HOGENOMiHOG000165752.
    KOiK00088.
    OMAiMREYRIS.
    OrthoDBiEOG793BHK.

    Family and domain databases

    Gene3Di3.20.20.70. 1 hit.
    HAMAPiMF_01964. IMPDH.
    InterProiIPR013785. Aldolase_TIM.
    IPR000644. CBS_dom.
    IPR005990. IMP_DH.
    IPR015875. IMP_DH/GMP_Rdtase_CS.
    IPR001093. IMP_DH_GMPRt.
    [Graphical view]
    PANTHERiPTHR11911:SF6. PTHR11911:SF6. 1 hit.
    PfamiPF00571. CBS. 2 hits.
    PF00478. IMPDH. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000130. IMPDH. 1 hit.
    SMARTiSM00116. CBS. 2 hits.
    [Graphical view]
    TIGRFAMsiTIGR01302. IMP_dehydrog. 1 hit.
    PROSITEiPS51371. CBS. 2 hits.
    PS00487. IMP_DH_GMP_RED. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P38697-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAAIRDYKTA LDFTKSLPRP DGLSVQELMD SKIRGGLTYN DFLILPGLVD    50
    FASSEVSLQT KLTRNITLNI PLVSSPMDTV TESEMATFMA LLGGIGFIHH 100
    NCTPEDQADM VRRVKNYENG FINNPIVISP TTTVGEAKSM KEKYGFAGFP 150
    VTTDGKRNAK LVGVITSRDI QFVEDNSLLV QDVMTKNPVT GAQGITLSEG 200
    NEILKKIKKG RLLVVDEKGN LVSMLSRTDL MKNQNYPLAS KSANTKQLLC 250
    GASIGTMDAD KERLRLLVKA GLDVVILDSS QGNSIFELNM LKWVKESFPG 300
    LEVIAGNVVT REQAANLIAA GADGLRIGMG TGSICITQEV MACGRPQGTA 350
    VYNVCEFANQ FGVPCMADGG VQNIGHITKA LALGSSTVMM GGMLAGTTES 400
    PGEYFYQDGK RLKAYRGMGS IDAMQKTGTK GNASTSRYFS ESDSVLVAQG 450
    VSGAVVDKGS IKKFIPYLYN GLQHSCQDIG CRSLTLLKNN VQRGKVRFEF 500
    RTASAQLEGG VHNLHSYEKR LHN 523
    Length:523
    Mass (Da):56,530
    Last modified:February 1, 1995 - v1
    Checksum:i7CA3EC11238906B9
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U00029 Genomic DNA. Translation: AAB69728.1.
    BK006934 Genomic DNA. Translation: DAA06915.1.
    PIRiS48997.
    RefSeqiNP_012088.3. NM_001179347.3.

    Genome annotation databases

    EnsemblFungiiYHR216W; YHR216W; YHR216W.
    GeneIDi856626.
    KEGGisce:YHR216W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U00029 Genomic DNA. Translation: AAB69728.1 .
    BK006934 Genomic DNA. Translation: DAA06915.1 .
    PIRi S48997.
    RefSeqi NP_012088.3. NM_001179347.3.

    3D structure databases

    ProteinModelPortali P38697.
    SMRi P38697. Positions 2-521.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 36651. 61 interactions.
    DIPi DIP-2840N.
    IntActi P38697. 37 interactions.
    MINTi MINT-521280.
    STRINGi 4932.YHR216W.

    Proteomic databases

    MaxQBi P38697.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YHR216W ; YHR216W ; YHR216W .
    GeneIDi 856626.
    KEGGi sce:YHR216W.

    Organism-specific databases

    CYGDi YHR216w.
    SGDi S000001259. IMD2.

    Phylogenomic databases

    GeneTreei ENSGT00530000062923.
    HOGENOMi HOG000165752.
    KOi K00088.
    OMAi MREYRIS.
    OrthoDBi EOG793BHK.

    Enzyme and pathway databases

    UniPathwayi UPA00601 ; UER00295 .
    BioCyci YEAST:YHR216W-MONOMER.
    SABIO-RK P38697.

    Miscellaneous databases

    NextBioi 982568.

    Gene expression databases

    Genevestigatori P38697.

    Family and domain databases

    Gene3Di 3.20.20.70. 1 hit.
    HAMAPi MF_01964. IMPDH.
    InterProi IPR013785. Aldolase_TIM.
    IPR000644. CBS_dom.
    IPR005990. IMP_DH.
    IPR015875. IMP_DH/GMP_Rdtase_CS.
    IPR001093. IMP_DH_GMPRt.
    [Graphical view ]
    PANTHERi PTHR11911:SF6. PTHR11911:SF6. 1 hit.
    Pfami PF00571. CBS. 2 hits.
    PF00478. IMPDH. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000130. IMPDH. 1 hit.
    SMARTi SM00116. CBS. 2 hits.
    [Graphical view ]
    TIGRFAMsi TIGR01302. IMP_dehydrog. 1 hit.
    PROSITEi PS51371. CBS. 2 hits.
    PS00487. IMP_DH_GMP_RED. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    2. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    3. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    4. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    5. "Functional distinctions between IMP dehydrogenase genes in providing mycophenolate resistance and guanine prototrophy to yeast."
      Hyle J.W., Shaw R.J., Reines D.
      J. Biol. Chem. 278:28470-28478(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INDUCTION.
    6. "Detection of the mycophenolate-inhibited form of IMP dehydrogenase in vivo."
      McPhillips C.C., Hyle J.W., Reines D.
      Proc. Natl. Acad. Sci. U.S.A. 101:12171-12176(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, MUTAGENESIS OF CYS-335, SUBUNIT.
    7. "Dissection of the molecular basis of mycophenolate resistance in Saccharomyces cerevisiae."
      Jenks M.H., Reines D.
      Yeast 22:1181-1190(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, MUTAGENESIS OF SER-253.

    Entry informationi

    Entry nameiIMDH2_YEAST
    AccessioniPrimary (citable) accession number: P38697
    Secondary accession number(s): D3DLH1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1995
    Last sequence update: February 1, 1995
    Last modified: October 1, 2014
    This is version 136 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 7870 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families
    3. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    4. Yeast chromosome VIII
      Yeast (Saccharomyces cerevisiae) chromosome VIII: entries and gene names

    External Data

    Dasty 3