Reviewed, UniProtKB/Swiss-Prot P38697 (IMDH2_YEAST)

Names and origin

Protein names

Recommended name:
    Inosine-5'-monophosphate dehydrogenase IMD2
      Short name=IMP dehydrogenase
      Short name=IMPDH
      Short name=IMPD
    EC=1.1.1.205

Gene names

Name:	IMD2
Synonyms:	PUR5
Ordered Locus Names:	YHR216W

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	523 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	Inosine 5'-phosphate + NAD+ + H2O = xanthosine 5'-phosphate + NADH.
Pathway	Purine metabolism; XMP biosynthesis via de novo pathway; XMP from IMP: step 1/1.
Miscellaneous	Present with 7870 molecules/cell in log phase SD medium. Ref.2
Sequence similarities	Belongs to the IMPDH/GMPR family. Contains 2 CBS domains.

Ontologies

Keywords
Biological process	GMP biosynthesis Purine biosynthesis
Domain	CBS domain Repeat
Ligand	Metal-binding NAD Potassium
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	GMP biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW GTP biosynthetic process Traceable author statement. Source: SGD oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from direct assay. Source: SGD
Molecular function	IMP dehydrogenase activity Traceable author statement. Source: SGD potassium ion binding Inferred from electronic annotation. Source: UniProtKB-KW protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
	P43582	1	EBI-9186,EBI-22766
SRP1	Q02821	1	EBI-9186,EBI-1797

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 523	523	Inosine-5'-monophosphate dehydrogenase IMD2		PRO_0000093682
Regions
	Domain	121 – 183	63	CBS 1
	Domain	184 – 240	57	CBS 2
	Nucleotide binding	257 – 280	24	NAD By similarity
Sites
	Active site	335	1	Thioimidate intermediate By similarity
	Metal binding	330	1	Potassium; via carbonyl oxygen By similarity
	Metal binding	332	1	Potassium; via carbonyl oxygen By similarity
	Binding site	280	1	Inhibitor By similarity
	Binding site	337	1	Inhibitor By similarity
	Binding site	368	1	IMP By similarity
	Binding site	415	1	IMP By similarity

Sequences

	Sequence		Length	Mass (Da)
	P38697-1 [UniParc]. Last modified February 1, 1995. Version 1. Checksum: 7CA3EC11238906B9	FASTA	523	56,530
	10 20 30 40 50 60 MAAIRDYKTA LDFTKSLPRP DGLSVQELMD SKIRGGLTYN DFLILPGLVD FASSEVSLQT 70 80 90 100 110 120 KLTRNITLNI PLVSSPMDTV TESEMATFMA LLGGIGFIHH NCTPEDQADM VRRVKNYENG 130 140 150 160 170 180 FINNPIVISP TTTVGEAKSM KEKYGFAGFP VTTDGKRNAK LVGVITSRDI QFVEDNSLLV 190 200 210 220 230 240 QDVMTKNPVT GAQGITLSEG NEILKKIKKG RLLVVDEKGN LVSMLSRTDL MKNQNYPLAS 250 260 270 280 290 300 KSANTKQLLC GASIGTMDAD KERLRLLVKA GLDVVILDSS QGNSIFELNM LKWVKESFPG 310 320 330 340 350 360 LEVIAGNVVT REQAANLIAA GADGLRIGMG TGSICITQEV MACGRPQGTA VYNVCEFANQ 370 380 390 400 410 420 FGVPCMADGG VQNIGHITKA LALGSSTVMM GGMLAGTTES PGEYFYQDGK RLKAYRGMGS 430 440 450 460 470 480 IDAMQKTGTK GNASTSRYFS ESDSVLVAQG VSGAVVDKGS IKKFIPYLYN GLQHSCQDIG 490 500 510 520 CRSLTLLKNN VQRGKVRFEF RTASAQLEGG VHNLHSYEKR LHN

References

[1]	"Complete nucleotide sequence of Saccharomyces cerevisiae chromosome VIII." Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Dover J., Du Z., Favello A., Fulton L., Gattung S., Geisel C., Kirsten J., Kucaba T., Hillier L.W., Jier M., Johnston L., Langston Y., Latreille P. , Louis E.J., Macri C., Mardis E., Menezes S., Mouser L., Nhan M., Rifkin L., Riles L., St Peter H., Trevaskis E., Vaughan K., Vignati D., Wilcox L., Wohldman P., Waterston R., Wilson R., Vaudin M. Science 265:2077-2082(1994) [PubMed: 8091229] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U00029 Genomic DNA. Translation: AAB69728.1.
PIR	S48997.
RefSeq	NP_012088.1.
3D structure databases
SMR	P38697. Positions 16-506, 232-521.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-2840N.
IntAct	P38697. 50 interactions.
STRING	P38697.
Proteomic databases
PRIDE	P38697.
Genome annotation databases
Ensembl	YHR216W; YHR216W; YHR216W; Saccharomyces cerevisiae. [Genome view]
GeneID	856626.
KEGG	sce:YHR216W.
NMPDR	fig|4932.3.peg.3265.
Organism-specific databases
CYGD	YHR216w.
SGD	S000001259. IMD2.
Phylogenomic databases
eggNOG	fuNOG05159.
HOGENOM	HBG298985.
OMA	VSESAMA.
OrthoDB	EOG92Z66Q.
PhylomeDB	P38697.
Enzyme and pathway databases
BRENDA	1.1.1.205. 250.
Gene expression databases
ArrayExpress	P38697.
Genevestigator	P38697.
GermOnline	YHR216W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR013785. Aldolase_TIM. IPR000644. Cysta_beta_synth_core. IPR005990. IMP_DH. IPR015875. IMP_DH/GMP_Rdtase_CS. IPR001093. IMP_DH_GMPRt. IPR018529. IMP_DH_rel. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
Pfam	PF00571. CBS. 2 hits. PF00478. IMPDH. 1 hit. [Graphical view]
PIRSF	PIRSF000130. IMPDH. 1 hit.
SMART	SM00116. CBS. 2 hits. [Graphical view]
TIGRFAMs	TIGR01302. IMP_dehydrog. 1 hit.
PROSITE	PS51371. CBS. 2 hits. PS00487. IMP_DH_GMP_RED. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	982568.

Entry information

Entry name

IMDH2_YEAST

Accession

Primary (citable) accession number: P38697

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	February 1, 1995
Last modified:	February 9, 2010
This is version 91 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)