Centractin - Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

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P38696 (ARP1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified December 14, 2011. Version 88. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Centractin

Alternative name(s):
Actin-like protein
Actin-related protein 1

Gene names

Name:	ARP1
Synonyms:	ACT3, ACT5
Ordered Locus Names:	YHR129C

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	384 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Core component of the dynactin complex which assists cytoplasmic dynein by increasing its processivity and by regulation of its cargo binding By similarity. The dynactin complex is required for the spindle translocation late in anaphase and is involved in a cell wall synthesis checkpoint. ARP1 forms the backbone filament of the dynactin rod structure and serves as the scaffold for the remaining subunits. Required for proper orientation of the mitotic spindle. Ref.6 Ref.8
Subunit structure	Self-associates to form an actin-like filament of 8-10 monomers. Component of the dynactin complex composed of at least ARP1, JNM1, NIP100 and ARP10. Dynactin comprises a short rod of the ARP1 filament attached to ARP10 at its pointed-end and probably associated with the capping protein at its barbed-end. The rod is implicated in dynein cargo binding. A sidearm formed by NIP100 projects from the ARP1 filament and is implicated in motor binding By similarity.
Subcellular location	Cytoplasm › cytoskeleton Probable. Membrane. Note: Membrane-associated. Ref.11
Miscellaneous	Present with 1580 molecules/cell in log phase SD medium. Ref.7
Sequence similarities	Belongs to the actin family. ARP1 subfamily.

Ontologies

Keywords
Cellular component	Cytoplasm Cytoskeleton Dynein Membrane Microtubule
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	establishment of mitotic spindle orientation Inferred from mutant phenotype Ref.2. Source: SGD nuclear migration Inferred from mutant phenotype Ref.2. Source: SGD
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-KW dynactin complex Inferred from direct assay Ref.6. Source: SGD dynein complex Inferred from electronic annotation. Source: UniProtKB-KW membrane Inferred from electronic annotation. Source: UniProtKB-SubCell microtubule Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	motor activity Inferred from electronic annotation. Source: UniProtKB-KW protein binding Inferred from physical interaction. Source: IntAct structural constituent of cytoskeleton Inferred from physical interaction Ref.6. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct
ARP10	Q04549	5	EBI-2920,EBI-2977
JNM1	P36224	9	EBI-2920,EBI-9415
NIP100	P33420	4	EBI-2920,EBI-12049

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 384

384

Centractin

PRO_0000089066

Experimental info

Mutagenesis

D → A: Temperature-sensitive; when associated with A-6. Ref.10

Mutagenesis

D → A: Temperature-sensitive; when associated with A-2. Ref.10

Mutagenesis

K → A: Strongly reduces interaction with JNM1. Reduces nuclear migration in mitosis; when associated with 48-A-A-49. Ref.9 Ref.10

Mutagenesis

48 – 49

DK → AA: Strongly reduces interaction with JNM1. Reduces nuclear migration in mitosis; when associated with A-46.

Mutagenesis

K → A: Reduces spindle formation; when associated with A-75. Ref.9

Mutagenesis

R → A: Reduces spindle formation; when associated with A-73. Ref.9

Mutagenesis

79 – 80

KH → AA: Reduces nuclear migration in mitosis.

Mutagenesis

84 – 85

ED → AA: Reduces spindle formation. Strongly reduces interaction with JNM1. Reduces nuclear migration in mitosis; when associated with A-87.

Mutagenesis

D → A: Reduces spindle formation. Reduces nuclear migration in mitosis; when associated with 84-A-A-85. Ref.9

Mutagenesis

108 – 109

EH → AA: Reduces nuclear migration in mitosis.

Mutagenesis

133

E → A: Lethal. Strongly reduces interaction with JNM1; when associated with A-136. Ref.10

Mutagenesis

136

D → A: Lethal. Strongly reduces interaction with JNM1; when associated with A-133. Ref.10

Mutagenesis

162

D → A: Reduces spindle formation. Reduces nuclear migration in mitosis; when associated with A-165. Ref.9

Mutagenesis

165

E → A: Reduces spindle formation. Reduces nuclear migration in mitosis; when associated with A-162. Ref.9

Mutagenesis

185

R → A: Temperature-sensitive. Strongly reduces interaction with JNM1; when associated with A-187. Ref.10

Mutagenesis

187

D → A: Temperature-sensitive. Strongly reduces interaction with JNM1; when associated with A-185. Ref.10

Mutagenesis

214 – 216

ERE → AAA: Lethal. Reduces spindle formation. Reduces nuclear migration in mitosis. Alters complex assembly; when associated with A-219. Ref.9 Ref.10

Mutagenesis

219

R → A: Lethal; when associated with 214-A--A-216. Ref.10

Mutagenesis

222 – 224

KEK → AAA: Lethal. Reduces spindle formation. Reduces nuclear migration in mitosis. Ref.9 Ref.10

Mutagenesis

233 – 238

KKEEEK → AAAAAA: Temperature-sensitive. Strongly reduces interaction with JNM1. Ref.10

Mutagenesis

233 – 235

KKE → AAA: Reduces spindle formation. Ref.9

Mutagenesis

236 – 238

EEK → AAA: Reduces spindle formation. Ref.9

Mutagenesis

251

K → A: Lethal and dominant cold-sensitive. Reduces nuclear migration in mitosis; when associated with A-254. Ref.9 Ref.10

Mutagenesis

254

D → A: Lethal and dominant cold-sensitive. Reduces nuclear migration in mitosis; when associated with A-251. Ref.9 Ref.10

Mutagenesis

263 – 264

DR → AA: Reduces spindle formation.

Mutagenesis

266

R → A: Lethal. Reduces spindle formation. Reduces nuclear migration in mitosis; when associated with A-269. Ref.9 Ref.10

Mutagenesis

269

E → A: Lethal. Reduces spindle formation. Reduces nuclear migration in mitosis; when associated with A-266. Ref.9 Ref.10

Mutagenesis

294

K → A: Reduces nuclear migration in mitosis; when associated with A-296 and A-298. Ref.9

Mutagenesis

296

D → A: Reduces nuclear migration in mitosis; when associated with A-294 and A-298. Ref.9

Mutagenesis

298

D → A: Reduces nuclear migration in mitosis; when associated with A-294 and A-296. Ref.9

Mutagenesis

321 – 322

DR → AA: Lethal. Strongly reduces interaction with JNM1.

Mutagenesis

326

D → A: Temperature-sensitive. Reduces nuclear migration in mitosis. Strongly reduces interaction with JNM1; when associated with A-328. Ref.9 Ref.10

Mutagenesis

328

E → A: Temperature-sensitive. Reduces nuclear migration in mitosis. Strongly reduces interaction with JNM1; when associated with A-326. Ref.9 Ref.10

Mutagenesis

336

K → A: Strongly reduces interaction with JNM1; when associated with A-338. Ref.10

Mutagenesis

338

K → A: Strongly reduces interaction with JNM1; when associated with A-336. Ref.10

Mutagenesis

344 – 346

ERK → AAA: Temperature-sensitive. Reduces nuclear migration in mitosis. Strongly reduces interaction with JNM1. Ref.9 Ref.10

Mutagenesis

368 – 369

KK → AA: Reduces nuclear migration in mitosis; when associated with A-371. Ref.10

Mutagenesis

369

K → A: Lethal. Strongly reduces interaction with JNM1; when associated with A-371. Ref.10

Mutagenesis

371

D → A: Lethal. Strongly reduces interaction with JNM1; when associated with A-369. Ref.9 Ref.10

Mutagenesis

371

D → AA: Reduces nuclear migration in mitosis; when associated with 368-A-A-369. Ref.9 Ref.10

Mutagenesis

374 – 375

ED → AA: Temperature-sensitive. Reduces nuclear migration in mitosis. Strongly reduces interaction with JNM1; when associated with A-378.

Mutagenesis

378

R → A: Temperature-sensitive. Reduces nuclear migration in mitosis. Strongly reduces interaction with JNM1; when associated with 374-A-A-375. Ref.10

Sequence conflict

Q → P in AAT93103. Ref.5

Sequences

Sequence

Length

Mass (Da)

Tools

P38696 [UniParc].

Last modified February 1, 1995. Version 1.
Checksum: 98EEA90DC9497B81
Show »

FASTA

384

42,995

        10         20         30         40         50         60 
MDQLSDSYAL YNQPVVIDNG SGIIKAGFSG EERPKALEYC LVGNTKYDKV MLEGLQGDTF 

        70         80         90        100        110        120 
IGNNAQKLRG LLKLRYPIKH GVVEDWDSME LIWSYVLNEV LQLQNIGEHP LLITEAPMNP 

       130        140        150        160        170        180 
LKNREQMAQV LFETFDVSAL YVSNPAVLSL YASGRTTGCV VDCGEGYCST VPIYDGFALP 

       190        200        210        220        230        240 
ASMMRMDIGG ADITEQLQFQ LRKSAGVSLF SSSEREIVRT MKEKVCYLAK NIKKEEEKYL 

       250        260        270        280        290        300 
QGTQDLISTF KLPDGRCIEV GNDRYRAPEI LFSPQIIGLG YDGLSDMCMQ SIWKVDLDLR 

       310        320        330        340        350        360 
KPLLSSIILS GGTTTLKGFG DRMLWDLEAL TKGTSKIKII APSERKYTTW IGGSILTGLS 

       370        380 
TFQRLWTKKS DWLEDSTRVY SNLM

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"ACT3: a putative centractin homologue in S. cerevisiae is required for proper orientation of the mitotic spindle." Clark S.W., Meyer D.I. J. Cell Biol. 127:129-138(1994) [PubMed: 7929558] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 26109 / X2180.
[2]	"A yeast actin-related protein homologous to that in vertebrate dynactin complex is important for spindle orientation and nuclear migration." Muhua L., Karpova T.S., Cooper J.A. Cell 78:669-679(1994) [PubMed: 8069915] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"Complete nucleotide sequence of Saccharomyces cerevisiae chromosome VIII." Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Dover J., Du Z., Favello A., Fulton L., Gattung S., Geisel C., Kirsten J., Kucaba T., Hillier L.W., Jier M., Johnston L., Langston Y., Latreille P. Vaudin M. Science 265:2077-2082(1994) [PubMed: 8091229] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[4]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[5]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. LaBaer J. Genome Res. 17:536-543(2007) [PubMed: 17322287] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]	"The yeast dynactin complex is involved in partitioning the mitotic spindle between mother and daughter cells during anaphase B." Kahana J.A., Schlenstedt G., Evanchuk D.M., Geiser J.R., Hoyt M.A., Silver P.A. Mol. Biol. Cell 9:1741-1756(1998) [PubMed: 9658168] [Abstract] Cited for: IDENTIFICATION IN THE DYNACTIN COMPLEX, FUNCTION OF THE DYNACTIN COMPLEX.
[7]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[8]	"Dynactin is involved in a checkpoint to monitor cell wall synthesis in Saccharomyces cerevisiae." Suzuki M., Igarashi R., Sekiya M., Utsugi T., Morishita S., Yukawa M., Ohya Y. Nat. Cell Biol. 6:861-871(2004) [PubMed: 15311283] [Abstract] Cited for: FUNCTION OF THE DYNACTIN COMPLEX.
[9]	"Molecular dissection of ARP1 regions required for nuclear migration and cell wall integrity checkpoint functions in Saccharomyces cerevisiae." Igarashi R., Suzuki M., Nogami S., Ohya Y. Cell Struct. Funct. 30:57-67(2005) [PubMed: 16415535] [Abstract] Cited for: MUTAGENESIS OF LYS-46; 48-ASP-LYS-49; LYS-73; ARG-75; 79-LYS-HIS-80; 84-GLU-ASP-85; ASP-87; 108-GLU-HIS-109; ASP-162; GLU-165; 214-GLU--GLU-216; 222-LYS--LYS-224; 233-LYS--GLU-235; 236-GLU--LYS-238; LYS-251; ASP-254; 263-ASP-ARG-264; ARG-266; GLU-269; LYS-294; ASP-296; ASP-298; ASP-326; GLU-328; 344-GLU--LYS-346; 368-LYS-LYS-369 AND ASP-371.
[10]	"Alanine scanning of Arp1 delineates a putative binding site for Jnm1/dynamitin and Nip100/p150Glued." Clark S.W., Rose M.D. Mol. Biol. Cell 16:3999-4012(2005) [PubMed: 15975903] [Abstract] Cited for: INTERACTION WITH JNM1, MUTAGENESIS OF ASP-2; ASP-6; LYS-46; 48-ASP-LYS-49; 84-GLU-ASP-85; GLU-133; ASP-136; ARG-185; ASP-187; 214-GLU--GLU-216; ARG-219; 222-LYS--LYS-224; 233-LYS--LYS-238; LYS-251; ASP-254; ARG-266; GLU-269; 321-ASP-ARG-322; ASP-326; GLU-328; LYS-336; LYS-338; 344-GLU--LYS-346; LYS-369; ASP-371; 374-GLU-ASP-375 AND ARG-378.
[11]	"Arp10p is a pointed-end-associated component of yeast dynactin." Clark S.W., Rose M.D. Mol. Biol. Cell 17:738-748(2006) [PubMed: 16291862] [Abstract] Cited for: SUBCELLULAR LOCATION, SELF-ASSOCIATION, INTERACTION WITH ARP10 AND JNM1.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X79811 Genomic DNA. Translation: CAA56206.1. U10398 Genomic DNA. Translation: AAB68412.1. AY693084 Genomic DNA. Translation: AAT93103.1. BK006934 Genomic DNA. Translation: DAA06822.1.
PIR	S48973.
RefSeq	NP_011997.1. NM_001179259.1.
3D structure databases
ProteinModelPortal	P38696.
SMR	P38696. Positions 12-383.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-1205N.
IntAct	P38696. 15 interactions.
MINT	MINT-389717.
STRING	P38696.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YHR129C; YHR129C; YHR129C.
GeneID	856530.
KEGG	sce:YHR129C.
NMPDR	fig\|4932.3.peg.3158.
Organism-specific databases
CYGD	YHR129c.
SGD	S000001171. ARP1.
Phylogenomic databases
eggNOG	fuNOG07557.
GeneTree	EFGT00050000001097.
HOGENOM	HBG559892.
OMA	MCELLFE.
OrthoDB	EOG4X3M8S.
Gene expression databases
ArrayExpress	P38696.
Genevestigator	P38696.
GermOnline	YHR129C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR004000. Actin-like. IPR020902. Actin/actin-like_CS. [Graphical view]
PANTHER	PTHR11937. Actin_like. 1 hit.
Pfam	PF00022. Actin. 1 hit. [Graphical view]
PRINTS	PR00190. ACTIN.
SMART	SM00268. ACTIN. 1 hit. [Graphical view]
PROSITE	PS01132. ACTINS_ACT_LIKE. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	982304.

Entry information

Entry name

ARP1_YEAST

Accession

Primary (citable) accession number: P38696
Secondary accession number(s): D3DL78, Q6B1J6

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	February 1, 1995
Last modified:	December 14, 2011
This is version 88 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome VIII

Yeast (Saccharomyces cerevisiae) chromosome VIII: entries and gene names

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents