ID   PPAC_YEAST              Reviewed;         467 AA.
AC   P38693; D3DLH0;
DT   01-FEB-1995, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1995, sequence version 1.
DT   27-MAR-2024, entry version 161.
DE   RecName: Full=Acid phosphatase PHO12;
DE            EC=3.1.3.2;
DE   Flags: Precursor;
GN   Name=PHO12; OrderedLocusNames=YHR215W;
OS   Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX   NCBI_TaxID=559292;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=8091229; DOI=10.1126/science.8091229;
RA   Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Dover J., Du Z.,
RA   Favello A., Fulton L., Gattung S., Geisel C., Kirsten J., Kucaba T.,
RA   Hillier L.W., Jier M., Johnston L., Langston Y., Latreille P., Louis E.J.,
RA   Macri C., Mardis E., Menezes S., Mouser L., Nhan M., Rifkin L., Riles L.,
RA   St Peter H., Trevaskis E., Vaughan K., Vignati D., Wilcox L., Wohldman P.,
RA   Waterston R., Wilson R., Vaudin M.;
RT   "Complete nucleotide sequence of Saccharomyces cerevisiae chromosome
RT   VIII.";
RL   Science 265:2077-2082(1994).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=24374639; DOI=10.1534/g3.113.008995;
RA   Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA   Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA   Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT   "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL   G3 (Bethesda) 4:389-398(2014).
RN   [3]
RP   LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX   PubMed=14562106; DOI=10.1038/nature02046;
RA   Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA   O'Shea E.K., Weissman J.S.;
RT   "Global analysis of protein expression in yeast.";
RL   Nature 425:737-741(2003).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a phosphate monoester + H2O = an alcohol + phosphate;
CC         Xref=Rhea:RHEA:15017, ChEBI:CHEBI:15377, ChEBI:CHEBI:30879,
CC         ChEBI:CHEBI:43474, ChEBI:CHEBI:67140; EC=3.1.3.2;
CC   -!- INDUCTION: S.cerevisiae has 2 types of acid phosphatase: one is
CC       constitutive and the other is repressible by inorganic phosphate.
CC   -!- PTM: Glycosylated during secretion across the membrane.
CC   -!- MISCELLANEOUS: Present with 3290 molecules/cell in log phase SD medium.
CC       {ECO:0000269|PubMed:14562106}.
CC   -!- SIMILARITY: Belongs to the histidine acid phosphatase family.
CC       {ECO:0000305}.
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DR   EMBL; U00029; AAB69729.1; -; Genomic_DNA.
DR   EMBL; BK006934; DAA06914.1; -; Genomic_DNA.
DR   PIR; S48996; S48996.
DR   RefSeq; NP_012087.1; NM_001179346.1.
DR   AlphaFoldDB; P38693; -.
DR   SMR; P38693; -.
DR   BioGRID; 36650; 26.
DR   DIP; DIP-1325N; -.
DR   IntAct; P38693; 2.
DR   MINT; P38693; -.
DR   STRING; 4932.YHR215W; -.
DR   GlyCosmos; P38693; 10 sites, No reported glycans.
DR   GlyGen; P38693; 10 sites.
DR   MaxQB; P38693; -.
DR   PaxDb; 4932-YHR215W; -.
DR   PeptideAtlas; P38693; -.
DR   EnsemblFungi; YHR215W_mRNA; YHR215W; YHR215W.
DR   GeneID; 856625; -.
DR   KEGG; sce:YHR215W; -.
DR   AGR; SGD:S000001258; -.
DR   SGD; S000001258; PHO12.
DR   VEuPathDB; FungiDB:YHR215W; -.
DR   eggNOG; KOG1382; Eukaryota.
DR   GeneTree; ENSGT00390000018409; -.
DR   HOGENOM; CLU_020880_3_1_1; -.
DR   InParanoid; P38693; -.
DR   OMA; LWINDGW; -.
DR   OrthoDB; 2404758at2759; -.
DR   BioCyc; YEAST:YHR215W-MONOMER; -.
DR   BioGRID-ORCS; 856625; 0 hits in 10 CRISPR screens.
DR   PRO; PR:P38693; -.
DR   Proteomes; UP000002311; Chromosome VIII.
DR   RNAct; P38693; Protein.
DR   GO; GO:0009277; C:fungal-type cell wall; IBA:GO_Central.
DR   GO; GO:0000324; C:fungal-type vacuole; HDA:SGD.
DR   GO; GO:0003993; F:acid phosphatase activity; IDA:SGD.
DR   CDD; cd07061; HP_HAP_like; 1.
DR   Gene3D; 3.40.50.1240; Phosphoglycerate mutase-like; 1.
DR   InterPro; IPR033379; Acid_Pase_AS.
DR   InterPro; IPR000560; His_Pase_clade-2.
DR   InterPro; IPR029033; His_PPase_superfam.
DR   InterPro; IPR016274; Histidine_acid_Pase_euk.
DR   PANTHER; PTHR20963:SF18; ACID PHOSPHATASE PHO11-RELATED; 1.
DR   PANTHER; PTHR20963; MULTIPLE INOSITOL POLYPHOSPHATE PHOSPHATASE-RELATED; 1.
DR   Pfam; PF00328; His_Phos_2; 1.
DR   PIRSF; PIRSF000894; Acid_phosphatase; 1.
DR   SUPFAM; SSF53254; Phosphoglycerate mutase-like; 1.
DR   PROSITE; PS00616; HIS_ACID_PHOSPHAT_1; 1.
DR   PROSITE; PS00778; HIS_ACID_PHOSPHAT_2; 1.
PE   1: Evidence at protein level;
KW   Glycoprotein; Hydrolase; Reference proteome; Signal.
FT   SIGNAL          1..17
FT                   /evidence="ECO:0000250"
FT   CHAIN           18..467
FT                   /note="Acid phosphatase PHO12"
FT                   /id="PRO_0000023957"
FT   ACT_SITE        75
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        338
FT                   /note="Proton donor"
FT                   /evidence="ECO:0000250"
FT   CARBOHYD        97
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        162
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        192
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        250
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        315
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        356
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        390
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        439
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        445
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        461
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   467 AA;  52700 MW;  BEC606CDF39B845B CRC64;
     MLKSAVYSIL AASLVNAGTI PLGKLSDIDK IGTQTEIFPF LGGSGPYYSF PGDYGISRDL
     PESCEMKQVQ MVGRHGERYP TVSKAKSIMT TWYKLSNYTG QFSGALSFLN DDYEFFIRDT
     KNLEMETTLA NSVNVLNPYT GEMNAKRHAR DFLAQYGYMV ENQTSFAVFT SNSNRCHDTA
     QYFIDGLGDK FNISLQTISE AESAGANTLS AHHSCPAWDD DVNDDILKKY DTKYLSGIAK
     RLNKENKGLN LTSSDANTFF AWCAYEINAR GYSDICNIFT KDELVRFSYG QDLETYYQTG
     PGYDVVRSVG ANLFNASVKL LKESEVQDQK VWLSFTHDTD ILNYLTTIGI IDDQNNLTAE
     HVPFMENTFH RSWYVPQGAR VYTEKFQCSN DTYVRYVIND AVVPIETCST GPGFSCEIND
     FYGYAEKRVA GTDFLKVCNV SSVSNSTELT FFWDWNTKHY NDTLLKQ
//