Reviewed, UniProtKB/Swiss-Prot P38693 (PPAC_YEAST)

Names and origin

Protein names

Recommended name:
    Acid phosphatase PHO12
    EC=3.1.3.2

Gene names

Name:	PHO12
Ordered Locus Names:	YHR215W

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	467 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	A phosphate monoester + H2O = an alcohol + phosphate.
Induction	S.cerevisiae has 2 types of acid phosphatase: one is constitutive and the other is repressible by inorganic phosphate.
Post-translational modification	Glycosylated during secretion across the membrane.
Miscellaneous	Present with 3290 molecules/cell in log phase SD medium. Ref.2
Sequence similarities	Belongs to the histidine acid phosphatase family.

Ontologies

Keywords
Domain	Signal
Molecular function	Hydrolase
PTM	Glycoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Cellular component	fungal-type vacuole Inferred from direct assay. Source: SGD
Molecular function	acid phosphatase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 17	17	By similarity
	Chain	18 – 467	450	Acid phosphatase PHO12		PRO_0000023957
Sites
	Active site	75	1	Nucleophile By similarity
	Active site	338	1	Proton donor By similarity
Amino acid modifications
	Glycosylation	97	1	N-linked (GlcNAc...) Potential
	Glycosylation	162	1	N-linked (GlcNAc...) Potential
	Glycosylation	192	1	N-linked (GlcNAc...) Potential
	Glycosylation	250	1	N-linked (GlcNAc...) Potential
	Glycosylation	315	1	N-linked (GlcNAc...) Potential
	Glycosylation	356	1	N-linked (GlcNAc...) Potential
	Glycosylation	390	1	N-linked (GlcNAc...) Potential
	Glycosylation	439	1	N-linked (GlcNAc...) Potential
	Glycosylation	445	1	N-linked (GlcNAc...) Potential
	Glycosylation	461	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	P38693-1 [UniParc]. Last modified February 1, 1995. Version 1. Checksum: BEC606CDF39B845B	FASTA	467	52,700
	10 20 30 40 50 60 MLKSAVYSIL AASLVNAGTI PLGKLSDIDK IGTQTEIFPF LGGSGPYYSF PGDYGISRDL 70 80 90 100 110 120 PESCEMKQVQ MVGRHGERYP TVSKAKSIMT TWYKLSNYTG QFSGALSFLN DDYEFFIRDT 130 140 150 160 170 180 KNLEMETTLA NSVNVLNPYT GEMNAKRHAR DFLAQYGYMV ENQTSFAVFT SNSNRCHDTA 190 200 210 220 230 240 QYFIDGLGDK FNISLQTISE AESAGANTLS AHHSCPAWDD DVNDDILKKY DTKYLSGIAK 250 260 270 280 290 300 RLNKENKGLN LTSSDANTFF AWCAYEINAR GYSDICNIFT KDELVRFSYG QDLETYYQTG 310 320 330 340 350 360 PGYDVVRSVG ANLFNASVKL LKESEVQDQK VWLSFTHDTD ILNYLTTIGI IDDQNNLTAE 370 380 390 400 410 420 HVPFMENTFH RSWYVPQGAR VYTEKFQCSN DTYVRYVIND AVVPIETCST GPGFSCEIND 430 440 450 460 FYGYAEKRVA GTDFLKVCNV SSVSNSTELT FFWDWNTKHY NDTLLKQ

References

[1]	"Complete nucleotide sequence of Saccharomyces cerevisiae chromosome VIII." Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Dover J., Du Z., Favello A., Fulton L., Gattung S., Geisel C., Kirsten J., Kucaba T., Hillier L.W., Jier M., Johnston L., Langston Y., Latreille P. , Louis E.J., Macri C., Mardis E., Menezes S., Mouser L., Nhan M., Rifkin L., Riles L., St Peter H., Trevaskis E., Vaughan K., Vignati D., Wilcox L., Wohldman P., Waterston R., Wilson R., Vaudin M. Science 265:2077-2082(1994) [PubMed: 8091229] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U00029 Genomic DNA. Translation: AAB69729.1.
PIR	S48996.
RefSeq	NP_012087.1.
3D structure databases
HSSP	HSSP built from PDB template 1QFX based on UniProtKB P34755.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:1325N.
IntAct	P38693. 5 interactions.
STRING	P38693.
Proteomic databases
PeptideAtlas	P38693.
Genome annotation databases
Ensembl	YHR215W; YHR215W; YHR215W; Saccharomyces cerevisiae. [Genome view]
GeneID	856625.
GenomeReviews	Gene locus YHR215W in contig U00093_GR.
KEGG	sce:YHR215W.
Organism-specific databases
CYGD	YHR215w.
SGD	S000001258. PHO12.
Phylogenomic databases
HOGENOM	P38693.
Enzyme and pathway databases
BRENDA	3.1.3.2. 250.
Gene expression databases
Genevestigator	P38693.
GermOnline	YHR215W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR000560. Histidine_acid_Pase. IPR016274. Histidine_acid_Pase_euk. [Graphical view]
Pfam	PF00328. Acid_phosphat_A. 1 hit. [Graphical view]
PIRSF	PIRSF000894. Acid_phosphatase. 1 hit.
PROSITE	PS00616. HIS_ACID_PHOSPHAT_1. 1 hit. PS00778. HIS_ACID_PHOSPHAT_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	982565.

Entry information

Entry name

PPAC_YEAST

Accession

Primary (citable) accession number: P38693

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	February 1, 1995
Last modified:	October 13, 2009
This is version 70 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)