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Protein

Acid phosphatase PHO12

Gene

PHO12

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

A phosphate monoester + H2O = an alcohol + phosphate.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei75NucleophileBy similarity1
Active sitei338Proton donorBy similarity1

GO - Molecular functioni

  • acid phosphatase activity Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Enzyme and pathway databases

BioCyciYEAST:YHR215W-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Acid phosphatase PHO12 (EC:3.1.3.2)
Gene namesi
Name:PHO12
Ordered Locus Names:YHR215W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VIII

Organism-specific databases

EuPathDBiFungiDB:YHR215W.
SGDiS000001258. PHO12.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 17By similarityAdd BLAST17
ChainiPRO_000002395718 – 467Acid phosphatase PHO12Add BLAST450

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi97N-linked (GlcNAc...)Sequence analysis1
Glycosylationi162N-linked (GlcNAc...)Sequence analysis1
Glycosylationi192N-linked (GlcNAc...)Sequence analysis1
Glycosylationi250N-linked (GlcNAc...)Sequence analysis1
Glycosylationi315N-linked (GlcNAc...)Sequence analysis1
Glycosylationi356N-linked (GlcNAc...)Sequence analysis1
Glycosylationi390N-linked (GlcNAc...)Sequence analysis1
Glycosylationi439N-linked (GlcNAc...)Sequence analysis1
Glycosylationi445N-linked (GlcNAc...)Sequence analysis1
Glycosylationi461N-linked (GlcNAc...)Sequence analysis1

Post-translational modificationi

Glycosylated during secretion across the membrane.

Keywords - PTMi

Glycoprotein

Proteomic databases

MaxQBiP38693.
PRIDEiP38693.

Expressioni

Inductioni

S.cerevisiae has 2 types of acid phosphatase: one is constitutive and the other is repressible by inorganic phosphate.

Interactioni

Protein-protein interaction databases

BioGridi36650. 7 interactors.
DIPiDIP-1325N.
IntActiP38693. 3 interactors.
MINTiMINT-411702.

Structurei

3D structure databases

ProteinModelPortaliP38693.
SMRiP38693.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the histidine acid phosphatase family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

HOGENOMiHOG000189191.
InParanoidiP38693.
KOiK01078.
OrthoDBiEOG092C2L5B.

Family and domain databases

CDDicd07061. HP_HAP_like. 1 hit.
Gene3Di3.40.50.1240. 1 hit.
InterProiIPR033379. Acid_Pase_AS.
IPR000560. His_Pase_clade-2.
IPR029033. His_PPase_superfam.
IPR016274. Histidine_acid_Pase_euk.
[Graphical view]
PfamiPF00328. His_Phos_2. 1 hit.
[Graphical view]
PIRSFiPIRSF000894. Acid_phosphatase. 1 hit.
SUPFAMiSSF53254. SSF53254. 1 hit.
PROSITEiPS00616. HIS_ACID_PHOSPHAT_1. 1 hit.
PS00778. HIS_ACID_PHOSPHAT_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P38693-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLKSAVYSIL AASLVNAGTI PLGKLSDIDK IGTQTEIFPF LGGSGPYYSF
60 70 80 90 100
PGDYGISRDL PESCEMKQVQ MVGRHGERYP TVSKAKSIMT TWYKLSNYTG
110 120 130 140 150
QFSGALSFLN DDYEFFIRDT KNLEMETTLA NSVNVLNPYT GEMNAKRHAR
160 170 180 190 200
DFLAQYGYMV ENQTSFAVFT SNSNRCHDTA QYFIDGLGDK FNISLQTISE
210 220 230 240 250
AESAGANTLS AHHSCPAWDD DVNDDILKKY DTKYLSGIAK RLNKENKGLN
260 270 280 290 300
LTSSDANTFF AWCAYEINAR GYSDICNIFT KDELVRFSYG QDLETYYQTG
310 320 330 340 350
PGYDVVRSVG ANLFNASVKL LKESEVQDQK VWLSFTHDTD ILNYLTTIGI
360 370 380 390 400
IDDQNNLTAE HVPFMENTFH RSWYVPQGAR VYTEKFQCSN DTYVRYVIND
410 420 430 440 450
AVVPIETCST GPGFSCEIND FYGYAEKRVA GTDFLKVCNV SSVSNSTELT
460
FFWDWNTKHY NDTLLKQ
Length:467
Mass (Da):52,700
Last modified:February 1, 1995 - v1
Checksum:iBEC606CDF39B845B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00029 Genomic DNA. Translation: AAB69729.1.
BK006934 Genomic DNA. Translation: DAA06914.1.
PIRiS48996.
RefSeqiNP_012087.1. NM_001179346.1.

Genome annotation databases

EnsemblFungiiYHR215W; YHR215W; YHR215W.
GeneIDi856625.
KEGGisce:YHR215W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00029 Genomic DNA. Translation: AAB69729.1.
BK006934 Genomic DNA. Translation: DAA06914.1.
PIRiS48996.
RefSeqiNP_012087.1. NM_001179346.1.

3D structure databases

ProteinModelPortaliP38693.
SMRiP38693.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi36650. 7 interactors.
DIPiDIP-1325N.
IntActiP38693. 3 interactors.
MINTiMINT-411702.

Proteomic databases

MaxQBiP38693.
PRIDEiP38693.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYHR215W; YHR215W; YHR215W.
GeneIDi856625.
KEGGisce:YHR215W.

Organism-specific databases

EuPathDBiFungiDB:YHR215W.
SGDiS000001258. PHO12.

Phylogenomic databases

HOGENOMiHOG000189191.
InParanoidiP38693.
KOiK01078.
OrthoDBiEOG092C2L5B.

Enzyme and pathway databases

BioCyciYEAST:YHR215W-MONOMER.

Miscellaneous databases

PROiP38693.

Family and domain databases

CDDicd07061. HP_HAP_like. 1 hit.
Gene3Di3.40.50.1240. 1 hit.
InterProiIPR033379. Acid_Pase_AS.
IPR000560. His_Pase_clade-2.
IPR029033. His_PPase_superfam.
IPR016274. Histidine_acid_Pase_euk.
[Graphical view]
PfamiPF00328. His_Phos_2. 1 hit.
[Graphical view]
PIRSFiPIRSF000894. Acid_phosphatase. 1 hit.
SUPFAMiSSF53254. SSF53254. 1 hit.
PROSITEiPS00616. HIS_ACID_PHOSPHAT_1. 1 hit.
PS00778. HIS_ACID_PHOSPHAT_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPPAC_YEAST
AccessioniPrimary (citable) accession number: P38693
Secondary accession number(s): D3DLH0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: November 2, 2016
This is version 125 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 3290 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome VIII
    Yeast (Saccharomyces cerevisiae) chromosome VIII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.