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Protein

Acid phosphatase PHO12

Gene

PHO12

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

A phosphate monoester + H2O = an alcohol + phosphate.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei75 – 751NucleophileBy similarity
Active sitei338 – 3381Proton donorBy similarity

GO - Molecular functioni

  1. acid phosphatase activity Source: SGD

GO - Biological processi

  1. dephosphorylation Source: GOC
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Enzyme and pathway databases

BioCyciYEAST:YHR215W-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Acid phosphatase PHO12 (EC:3.1.3.2)
Gene namesi
Name:PHO12
Ordered Locus Names:YHR215W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome VIII

Organism-specific databases

CYGDiYHR215w.
SGDiS000001258. PHO12.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1717By similarityAdd
BLAST
Chaini18 – 467450Acid phosphatase PHO12PRO_0000023957Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi97 – 971N-linked (GlcNAc...)Sequence Analysis
Glycosylationi162 – 1621N-linked (GlcNAc...)Sequence Analysis
Glycosylationi192 – 1921N-linked (GlcNAc...)Sequence Analysis
Glycosylationi250 – 2501N-linked (GlcNAc...)Sequence Analysis
Glycosylationi315 – 3151N-linked (GlcNAc...)Sequence Analysis
Glycosylationi356 – 3561N-linked (GlcNAc...)Sequence Analysis
Glycosylationi390 – 3901N-linked (GlcNAc...)Sequence Analysis
Glycosylationi439 – 4391N-linked (GlcNAc...)Sequence Analysis
Glycosylationi445 – 4451N-linked (GlcNAc...)Sequence Analysis
Glycosylationi461 – 4611N-linked (GlcNAc...)Sequence Analysis

Post-translational modificationi

Glycosylated during secretion across the membrane.

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiP38693.
PeptideAtlasiP38693.

Expressioni

Inductioni

S.cerevisiae has 2 types of acid phosphatase: one is constitutive and the other is repressible by inorganic phosphate.

Gene expression databases

GenevestigatoriP38693.

Interactioni

Protein-protein interaction databases

BioGridi36650. 7 interactions.
DIPiDIP-1325N.
IntActiP38693. 3 interactions.
MINTiMINT-411702.
STRINGi4932.YHR215W.

Structurei

3D structure databases

ProteinModelPortaliP38693.
SMRiP38693. Positions 37-457.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the histidine acid phosphatase family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiNOG260296.
GeneTreeiENSGT00510000050349.
HOGENOMiHOG000189191.
InParanoidiP38693.
KOiK01078.
OrthoDBiEOG7TQV9N.

Family and domain databases

Gene3Di3.40.50.1240. 1 hit.
InterProiIPR000560. His_Pase_superF_clade-2.
IPR029033. His_PPase_superfam.
IPR016274. Histidine_acid_Pase_euk.
[Graphical view]
PfamiPF00328. His_Phos_2. 1 hit.
[Graphical view]
PIRSFiPIRSF000894. Acid_phosphatase. 1 hit.
SUPFAMiSSF53254. SSF53254. 1 hit.
PROSITEiPS00616. HIS_ACID_PHOSPHAT_1. 1 hit.
PS00778. HIS_ACID_PHOSPHAT_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P38693-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLKSAVYSIL AASLVNAGTI PLGKLSDIDK IGTQTEIFPF LGGSGPYYSF
60 70 80 90 100
PGDYGISRDL PESCEMKQVQ MVGRHGERYP TVSKAKSIMT TWYKLSNYTG
110 120 130 140 150
QFSGALSFLN DDYEFFIRDT KNLEMETTLA NSVNVLNPYT GEMNAKRHAR
160 170 180 190 200
DFLAQYGYMV ENQTSFAVFT SNSNRCHDTA QYFIDGLGDK FNISLQTISE
210 220 230 240 250
AESAGANTLS AHHSCPAWDD DVNDDILKKY DTKYLSGIAK RLNKENKGLN
260 270 280 290 300
LTSSDANTFF AWCAYEINAR GYSDICNIFT KDELVRFSYG QDLETYYQTG
310 320 330 340 350
PGYDVVRSVG ANLFNASVKL LKESEVQDQK VWLSFTHDTD ILNYLTTIGI
360 370 380 390 400
IDDQNNLTAE HVPFMENTFH RSWYVPQGAR VYTEKFQCSN DTYVRYVIND
410 420 430 440 450
AVVPIETCST GPGFSCEIND FYGYAEKRVA GTDFLKVCNV SSVSNSTELT
460
FFWDWNTKHY NDTLLKQ
Length:467
Mass (Da):52,700
Last modified:February 1, 1995 - v1
Checksum:iBEC606CDF39B845B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00029 Genomic DNA. Translation: AAB69729.1.
BK006934 Genomic DNA. Translation: DAA06914.1.
PIRiS48996.
RefSeqiNP_012087.1. NM_001179346.1.

Genome annotation databases

EnsemblFungiiYHR215W; YHR215W; YHR215W.
GeneIDi856625.
KEGGisce:YHR215W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00029 Genomic DNA. Translation: AAB69729.1.
BK006934 Genomic DNA. Translation: DAA06914.1.
PIRiS48996.
RefSeqiNP_012087.1. NM_001179346.1.

3D structure databases

ProteinModelPortaliP38693.
SMRiP38693. Positions 37-457.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi36650. 7 interactions.
DIPiDIP-1325N.
IntActiP38693. 3 interactions.
MINTiMINT-411702.
STRINGi4932.YHR215W.

Proteomic databases

PaxDbiP38693.
PeptideAtlasiP38693.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYHR215W; YHR215W; YHR215W.
GeneIDi856625.
KEGGisce:YHR215W.

Organism-specific databases

CYGDiYHR215w.
SGDiS000001258. PHO12.

Phylogenomic databases

eggNOGiNOG260296.
GeneTreeiENSGT00510000050349.
HOGENOMiHOG000189191.
InParanoidiP38693.
KOiK01078.
OrthoDBiEOG7TQV9N.

Enzyme and pathway databases

BioCyciYEAST:YHR215W-MONOMER.

Miscellaneous databases

NextBioi982565.

Gene expression databases

GenevestigatoriP38693.

Family and domain databases

Gene3Di3.40.50.1240. 1 hit.
InterProiIPR000560. His_Pase_superF_clade-2.
IPR029033. His_PPase_superfam.
IPR016274. Histidine_acid_Pase_euk.
[Graphical view]
PfamiPF00328. His_Phos_2. 1 hit.
[Graphical view]
PIRSFiPIRSF000894. Acid_phosphatase. 1 hit.
SUPFAMiSSF53254. SSF53254. 1 hit.
PROSITEiPS00616. HIS_ACID_PHOSPHAT_1. 1 hit.
PS00778. HIS_ACID_PHOSPHAT_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiPPAC_YEAST
AccessioniPrimary (citable) accession number: P38693
Secondary accession number(s): D3DLH0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: January 7, 2015
This is version 113 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 3290 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome VIII
    Yeast (Saccharomyces cerevisiae) chromosome VIII: entries and gene names

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.