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Protein

Serine/threonine-protein kinase KSP1

Gene

KSP1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

May act on PRP20.

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei47 – 471ATPPROSITE-ProRule annotation
Active sitei207 – 2071Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi27 – 359ATPPROSITE-ProRule annotation

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • protein serine/threonine/tyrosine kinase activity Source: SGD
  • protein serine/threonine kinase activity Source: SGD

GO - Biological processi

  • cellular protein localization Source: SGD
  • negative regulation of macroautophagy Source: SGD
  • protein phosphorylation Source: SGD
  • regulation of filamentous growth Source: SGD
  • regulation of pseudohyphal growth Source: SGD
  • TOR signaling Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-31129-MONOMER.
BRENDAi2.7.11.1. 984.

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein kinase KSP1 (EC:2.7.11.1)
Gene namesi
Name:KSP1
Ordered Locus Names:YHR082C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VIII

Organism-specific databases

EuPathDBiFungiDB:YHR082C.
SGDiS000001124. KSP1.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: SGD
  • cytoplasmic stress granule Source: SGD
  • nucleus Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10291029Serine/threonine-protein kinase KSP1PRO_0000086228Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei416 – 4161PhosphoserineCombined sources
Modified residuei419 – 4191PhosphoserineCombined sources
Modified residuei501 – 5011PhosphothreonineCombined sources
Modified residuei504 – 5041PhosphothreonineCombined sources
Modified residuei526 – 5261PhosphothreonineCombined sources
Modified residuei529 – 5291PhosphoserineCombined sources
Modified residuei646 – 6461PhosphoserineCombined sources
Modified residuei845 – 8451PhosphoserineCombined sources
Modified residuei884 – 8841PhosphoserineCombined sources
Modified residuei1005 – 10051PhosphothreonineCombined sources
Modified residuei1014 – 10141PhosphoserineCombined sources

Post-translational modificationi

Phosphorylated by PKA in a TORC1-dependent manner. Phosphorylation at PKA consensus sites RRxS/T decreases upon rapamycin treatment.1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP38691.
PeptideAtlasiP38691.

PTM databases

iPTMnetiP38691.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
TOR1P351692EBI-9937,EBI-19374

Protein-protein interaction databases

BioGridi36517. 339 interactions.
DIPiDIP-2972N.
IntActiP38691. 18 interactions.
MINTiMINT-674742.

Structurei

3D structure databases

ProteinModelPortaliP38691.
SMRiP38691. Positions 77-420.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini18 – 351334Protein kinasePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CK2 subfamily.PROSITE-ProRule annotation
Contains 1 protein kinase domain.PROSITE-ProRule annotation

Phylogenomic databases

GeneTreeiENSGT00390000011998.
HOGENOMiHOG000000880.
InParanoidiP38691.
KOiK08286.
OMAiDSYIIME.
OrthoDBiEOG76X69X.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 2 hits.
PROSITEiPS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P38691-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTLDYEIYKE GGILNNRYQK IEDISEGSYG YVSLAKDVRE KRLVAVKYIF
60 70 80 90 100
KLEDDGQYDG PQDDENDCDS SDCDDDEDTK VDTDRHENEN GNASSNNGSS
110 120 130 140 150
REKKHNLYKH KKSLISSKVK SRLSNNICLE AMYEVDIQTK IGRHQNIAAL
160 170 180 190 200
LDFFDSYIIM EYCSGGDLYE AIKADAVPKK TKSITHIITQ IMDAIEYVHN
210 220 230 240 250
KGIYHRDIKP ENILISGIDW TIKLTDWGLA TTDKTSMDRN VGSERYMSPE
260 270 280 290 300
LFDSNLDIKE RKEPYDCAKV DLWAMGIVFL NIVFHKNPFS IANQSDKSFC
310 320 330 340 350
YFAANREALF DVFSTMAYDF FQVLRYSLTI DPANRDLKMM RTELQNLSEY
360 370 380 390 400
TLDDEYYNNL DEGYEETMID GLPPQPVPPS SAPVSLPTPI SSSNKQHMPE
410 420 430 440 450
FKKDFNFNNV NERKRSDVSQ NQNVASGFFK KPSTQQQKFF NQGYNTTLST
460 470 480 490 500
HERAKSAPKF KFKKRNKYGR TDNQFSKPVN IEDRKKSKIL KKSRKPLGIP
510 520 530 540 550
TPNTHMNNFF HDYKARDEFN TRDFFTPPSV QHRYMEGFSN NNNKQYRQNR
560 570 580 590 600
NYNNNNNNSN NNHGSNYNNF NNGNSYIKGW NKNFNKYRRP SSSSYTGKSP
610 620 630 640 650
LSRYNMSYNH NNNSSINGYA RRGSTTTVQH SPGAYIPPNA RNHHVSPTNQ
660 670 680 690 700
FLRVPQSTAP DISTVLGGKP SYQEHYTQDS MDSEGDHDSD DVLFTLEEGD
710 720 730 740 750
HDFVNGMDNL SINDHLPHTT VGSHNEVFVH ASTNHNNNGN NNHIDTNSTT
760 770 780 790 800
NQYHRQYIPP PLTTSLHINN NNNESNELPD LLKSPASSEA HLNLSSGPID
810 820 830 840 850
PILTGNIGNR YSHSSDSKEL EQERRLSMEQ KFKNGVYVPP HHRKSFNLGT
860 870 880 890 900
QVPPMNMKTS NEATLSVSHN SVNFGGSYNS RRSSANESNP LHMNKALEKL
910 920 930 940 950
SSSPGAKSSF VGFPKPLLPR NHSSTTIALQ NEDVFADSNN DAIIFEDEEY
960 970 980 990 1000
EGESDKMAHG KMEGGDNESS STSPDERQIF GPYEIYAQTF AGSTHDKKLG
1010 1020
AGRKTSIQDE MVGSLEQYKN NWLILQQQD
Length:1,029
Mass (Da):117,082
Last modified:February 1, 1995 - v1
Checksum:i99E2A1FC7CEB3D5A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X80329 Genomic DNA. Translation: CAA56578.1.
U10556 Genomic DNA. Translation: AAB68896.1.
BK006934 Genomic DNA. Translation: DAA06777.1.
PIRiS64731.
RefSeqiNP_011950.1. NM_001179212.1.

Genome annotation databases

EnsemblFungiiYHR082C; YHR082C; YHR082C.
GeneIDi856482.
KEGGisce:YHR082C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X80329 Genomic DNA. Translation: CAA56578.1.
U10556 Genomic DNA. Translation: AAB68896.1.
BK006934 Genomic DNA. Translation: DAA06777.1.
PIRiS64731.
RefSeqiNP_011950.1. NM_001179212.1.

3D structure databases

ProteinModelPortaliP38691.
SMRiP38691. Positions 77-420.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi36517. 339 interactions.
DIPiDIP-2972N.
IntActiP38691. 18 interactions.
MINTiMINT-674742.

PTM databases

iPTMnetiP38691.

Proteomic databases

MaxQBiP38691.
PeptideAtlasiP38691.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYHR082C; YHR082C; YHR082C.
GeneIDi856482.
KEGGisce:YHR082C.

Organism-specific databases

EuPathDBiFungiDB:YHR082C.
SGDiS000001124. KSP1.

Phylogenomic databases

GeneTreeiENSGT00390000011998.
HOGENOMiHOG000000880.
InParanoidiP38691.
KOiK08286.
OMAiDSYIIME.
OrthoDBiEOG76X69X.

Enzyme and pathway databases

BioCyciYEAST:G3O-31129-MONOMER.
BRENDAi2.7.11.1. 984.

Miscellaneous databases

NextBioi982170.
PROiP38691.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 2 hits.
PROSITEiPS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Allele-specific suppression of a Saccharomyces cerevisiae prp20 mutation by overexpression of a nuclear serine/threonine protein kinase."
    Fleischmann M., Stagljar I., Aebi M.
    Mol. Gen. Genet. 250:614-625(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: M335 /2A.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  5. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
    Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
    J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-416; THR-526; SER-646 AND SER-845, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: ADR376.
  6. "Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry."
    Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F.
    Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  7. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-526; SER-529; SER-845 AND SER-1014, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  8. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-416; SER-419; THR-501; THR-504; SER-646; SER-845; SER-884; THR-1005 AND SER-1014, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  9. "The rapamycin-sensitive phosphoproteome reveals that TOR controls protein kinase A toward some but not all substrates."
    Soulard A., Cremonesi A., Moes S., Schutz F., Jeno P., Hall M.N.
    Mol. Biol. Cell 21:3475-3486(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION BY PKA.

Entry informationi

Entry nameiKSP1_YEAST
AccessioniPrimary (citable) accession number: P38691
Secondary accession number(s): D3DL33
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: May 11, 2016
This is version 152 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 1270 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome VIII
    Yeast (Saccharomyces cerevisiae) chromosome VIII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.