ID   YHJ3_YEAST              Reviewed;         423 AA.
AC   P38690; D3DKY0; Q66R86;
DT   01-FEB-1995, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1995, sequence version 1.
DT   27-MAR-2024, entry version 164.
DE   RecName: Full=Uncharacterized protein YHR033W;
GN   OrderedLocusNames=YHR033W;
OS   Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX   NCBI_TaxID=559292;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=8091229; DOI=10.1126/science.8091229;
RA   Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Dover J., Du Z.,
RA   Favello A., Fulton L., Gattung S., Geisel C., Kirsten J., Kucaba T.,
RA   Hillier L.W., Jier M., Johnston L., Langston Y., Latreille P., Louis E.J.,
RA   Macri C., Mardis E., Menezes S., Mouser L., Nhan M., Rifkin L., Riles L.,
RA   St Peter H., Trevaskis E., Vaughan K., Vignati D., Wilcox L., Wohldman P.,
RA   Waterston R., Wilson R., Vaudin M.;
RT   "Complete nucleotide sequence of Saccharomyces cerevisiae chromosome
RT   VIII.";
RL   Science 265:2077-2082(1994).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=24374639; DOI=10.1534/g3.113.008995;
RA   Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA   Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA   Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT   "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL   G3 (Bethesda) 4:389-398(2014).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=17322287; DOI=10.1101/gr.6037607;
RA   Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F.,
RA   Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J.,
RA   Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J.,
RA   Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D.,
RA   LaBaer J.;
RT   "Approaching a complete repository of sequence-verified protein-encoding
RT   clones for Saccharomyces cerevisiae.";
RL   Genome Res. 17:536-543(2007).
RN   [4]
RP   SUBCELLULAR LOCATION.
RX   PubMed=11914276; DOI=10.1101/gad.970902;
RA   Kumar A., Agarwal S., Heyman J.A., Matson S., Heidtman M., Piccirillo S.,
RA   Umansky L., Drawid A., Jansen R., Liu Y., Cheung K.-H., Miller P.,
RA   Gerstein M., Roeder G.S., Snyder M.;
RT   "Subcellular localization of the yeast proteome.";
RL   Genes Dev. 16:707-719(2002).
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:11914276}.
CC   -!- SIMILARITY: Belongs to the glutamate 5-kinase family. {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; U00062; AAB68910.1; -; Genomic_DNA.
DR   EMBL; AY723823; AAU09740.1; -; Genomic_DNA.
DR   EMBL; BK006934; DAA06724.1; -; Genomic_DNA.
DR   PIR; S46741; S46741.
DR   RefSeq; NP_011898.1; NM_001179163.1.
DR   AlphaFoldDB; P38690; -.
DR   SMR; P38690; -.
DR   BioGRID; 36464; 66.
DR   DIP; DIP-5036N; -.
DR   IntAct; P38690; 21.
DR   MINT; P38690; -.
DR   STRING; 4932.YHR033W; -.
DR   MaxQB; P38690; -.
DR   PaxDb; 4932-YHR033W; -.
DR   PeptideAtlas; P38690; -.
DR   EnsemblFungi; YHR033W_mRNA; YHR033W; YHR033W.
DR   GeneID; 856428; -.
DR   KEGG; sce:YHR033W; -.
DR   AGR; SGD:S000001075; -.
DR   SGD; S000001075; YHR033W.
DR   VEuPathDB; FungiDB:YHR033W; -.
DR   eggNOG; KOG1154; Eukaryota.
DR   GeneTree; ENSGT00500000044903; -.
DR   HOGENOM; CLU_025400_1_1_1; -.
DR   InParanoid; P38690; -.
DR   OMA; TNPRKDP; -.
DR   OrthoDB; 2021096at2759; -.
DR   BioCyc; YEAST:G3O-31093-MONOMER; -.
DR   BioGRID-ORCS; 856428; 3 hits in 10 CRISPR screens.
DR   PRO; PR:P38690; -.
DR   Proteomes; UP000002311; Chromosome VIII.
DR   RNAct; P38690; Protein.
DR   GO; GO:0005737; C:cytoplasm; HDA:SGD.
DR   GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0004349; F:glutamate 5-kinase activity; IBA:GO_Central.
DR   GO; GO:0003723; F:RNA binding; IEA:InterPro.
DR   GO; GO:0016310; P:phosphorylation; IEA:UniProtKB-KW.
DR   GO; GO:0006561; P:proline biosynthetic process; IBA:GO_Central.
DR   CDD; cd04242; AAK_G5K_ProB; 1.
DR   CDD; cd21157; PUA_G5K; 1.
DR   Gene3D; 3.40.1160.10; Acetylglutamate kinase-like; 2.
DR   Gene3D; 2.30.130.10; PUA domain; 1.
DR   HAMAP; MF_00456; ProB; 1.
DR   InterPro; IPR036393; AceGlu_kinase-like_sf.
DR   InterPro; IPR001048; Asp/Glu/Uridylate_kinase.
DR   InterPro; IPR041739; G5K_ProB.
DR   InterPro; IPR001057; Glu/AcGlu_kinase.
DR   InterPro; IPR011529; Glu_5kinase.
DR   InterPro; IPR005715; Glu_5kinase/COase_Synthase.
DR   InterPro; IPR019797; Glutamate_5-kinase_CS.
DR   InterPro; IPR002478; PUA.
DR   InterPro; IPR015947; PUA-like_sf.
DR   InterPro; IPR036974; PUA_sf.
DR   NCBIfam; TIGR01027; proB; 1.
DR   PANTHER; PTHR43654; GLUTAMATE 5-KINASE; 1.
DR   PANTHER; PTHR43654:SF3; GLUTAMATE 5-KINASE; 1.
DR   Pfam; PF00696; AA_kinase; 1.
DR   Pfam; PF01472; PUA; 1.
DR   PIRSF; PIRSF000729; GK; 1.
DR   PRINTS; PR00474; GLU5KINASE.
DR   SMART; SM00359; PUA; 1.
DR   SUPFAM; SSF53633; Carbamate kinase-like; 1.
DR   SUPFAM; SSF88697; PUA domain-like; 1.
DR   PROSITE; PS00902; GLUTAMATE_5_KINASE; 1.
DR   PROSITE; PS50890; PUA; 1.
PE   3: Inferred from homology;
KW   Amino-acid biosynthesis; ATP-binding; Cytoplasm; Kinase;
KW   Nucleotide-binding; Proline biosynthesis; Reference proteome; Transferase.
FT   CHAIN           1..423
FT                   /note="Uncharacterized protein YHR033W"
FT                   /id="PRO_0000109779"
FT   DOMAIN          315..406
FT                   /note="PUA"
FT   BINDING         51
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250"
FT   BINDING         138
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250"
FT   BINDING         150
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250"
FT   BINDING         170..171
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000250"
FT   BINDING         214..220
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000250"
FT   CONFLICT        117
FT                   /note="N -> D (in Ref. 3; AAU09740)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   423 AA;  46864 MW;  C7CBF7B1773F1981 CRC64;
     MTKAYTIVIK LGSSSLVDES TKEPKLSIMT LIVETVTNLK RMGHKVIIVS SGGIAVGLDA
     LNIPHKPKQL SEVQAIAAVG QGRLIARWNM LFSQYGEQTA QILLTRNDIL RWNQYNNARN
     TINELLAMGV IPIVNENDTL SISEIEFGDN DTLSAITAAL VGADFLFLLT DVDCLYTDNP
     RTNPDARPIV LVPELSEGLP GVNTSSGSGS EVGTGGMRTK LIAADLASNA GIETIVMKSD
     RPEYVPKIVD YIQHHFRPPR HIGNGTQQQF LDLQDTELEQ LRRYDVPMHT KFLANDNKHK
     LKNREFWILH GLITKGAIII DENSYDKLLS KDMASLTPNA VIEVRDNFHE LECVDLKIGQ
     RLPNGELDIS KPIQSVGCVR SNYTSLELAK IKGLPSEKIH DVLGYSVSEY VAHRENIAFP
     PQF
//