P38690 (YHJ3_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Uncharacterized protein YHR033W

Gene names

Ordered Locus Names:

YHR033W

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	423 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Subcellular location	Cytoplasm Ref.4.
Sequence similarities	Belongs to the glutamate 5-kinase family. Contains 1 PUA domain.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Proline biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	proline biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	cytoplasm Inferred from direct assay Ref.4. Source: SGD
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW RNA binding Inferred from electronic annotation. Source: InterPro glutamate 5-kinase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 423	423	Uncharacterized protein YHR033W		PRO_0000109779
Regions
	Domain	315 – 406	92	PUA
	Nucleotide binding	170 – 171	2	ATP By similarity
	Nucleotide binding	214 – 220	7	ATP By similarity
Sites
	Binding site	51	1	Substrate By similarity
	Binding site	138	1	Substrate By similarity
	Binding site	150	1	Substrate; via amide nitrogen By similarity
Experimental info
	Sequence conflict	117	1	N → D in AAU09740. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	P38690 [UniParc]. Last modified February 1, 1995. Version 1. Checksum: C7CBF7B1773F1981	FASTA	423	46,864
	10 20 30 40 50 60 MTKAYTIVIK LGSSSLVDES TKEPKLSIMT LIVETVTNLK RMGHKVIIVS SGGIAVGLDA 70 80 90 100 110 120 LNIPHKPKQL SEVQAIAAVG QGRLIARWNM LFSQYGEQTA QILLTRNDIL RWNQYNNARN 130 140 150 160 170 180 TINELLAMGV IPIVNENDTL SISEIEFGDN DTLSAITAAL VGADFLFLLT DVDCLYTDNP 190 200 210 220 230 240 RTNPDARPIV LVPELSEGLP GVNTSSGSGS EVGTGGMRTK LIAADLASNA GIETIVMKSD 250 260 270 280 290 300 RPEYVPKIVD YIQHHFRPPR HIGNGTQQQF LDLQDTELEQ LRRYDVPMHT KFLANDNKHK 310 320 330 340 350 360 LKNREFWILH GLITKGAIII DENSYDKLLS KDMASLTPNA VIEVRDNFHE LECVDLKIGQ 370 380 390 400 410 420 RLPNGELDIS KPIQSVGCVR SNYTSLELAK IKGLPSEKIH DVLGYSVSEY VAHRENIAFP PQF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete nucleotide sequence of Saccharomyces cerevisiae chromosome VIII." Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Dover J., Du Z., Favello A., Fulton L., Gattung S., Geisel C., Kirsten J., Kucaba T., Hillier L.W., Jier M., Johnston L., Langston Y., Latreille P. , Louis E.J., Macri C., Mardis E., Menezes S., Mouser L., Nhan M., Rifkin L., Riles L., St Peter H., Trevaskis E., Vaughan K., Vignati D., Wilcox L., Wohldman P., Waterston R., Wilson R., Vaudin M. Science 265:2077-2082(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[3]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[4]	"Subcellular localization of the yeast proteome." Kumar A., Agarwal S., Heyman J.A., Matson S., Heidtman M., Piccirillo S., Umansky L., Drawid A., Jansen R., Liu Y., Cheung K.-H., Miller P., Gerstein M., Roeder G.S., Snyder M. Genes Dev. 16:707-719(2002) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U00062 Genomic DNA. Translation: AAB68910.1. AY723823 Genomic DNA. Translation: AAU09740.1. BK006934 Genomic DNA. Translation: DAA06724.1.
PIR	S46741.
RefSeq	NP_011898.1. NM_001179163.1.
3D structure databases
ProteinModelPortal	P38690.
SMR	P38690. Positions 6-417.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-5036N.
IntAct	P38690. 20 interactions.
MINT	MINT-515715.
STRING	4932.YHR033W.
Proteomic databases
PaxDb	P38690.
PeptideAtlas	P38690.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YHR033W; YHR033W; YHR033W.
GeneID	856428.
KEGG	sce:YHR033W.
Organism-specific databases
CYGD	YHR033w.
SGD	S000001075. YHR033W.
Phylogenomic databases
eggNOG	COG0263.
GeneTree	ENSGT00550000075661.
HOGENOM	HOG000246369.
OMA	VSEYVAH.
OrthoDB	EOG4QVGMG.
Gene expression databases
Genevestigator	P38690.
GermOnline	YHR033W. Saccharomyces cerevisiae.
Family and domain databases
Gene3D	3.40.1160.10. 1 hit.
InterPro	IPR001048. Asp/Glu/Uridylate_kinase. IPR001057. Glu/AcGlu_kinase. IPR011529. Glu_5kinase. IPR005715. Glu_5kinase/COase_Synthase. IPR019797. Glutamate_5-kinase_CS. IPR002478. PUA. IPR015947. PUA-like_domain. [Graphical view]
Pfam	PF00696. AA_kinase. 1 hit. PF01472. PUA. 1 hit. [Graphical view]
PIRSF	PIRSF000729. GK. 1 hit.
PRINTS	PR00474. GLU5KINASE.
SMART	SM00359. PUA. 1 hit. [Graphical view]
SUPFAM	SSF53633. Aa_kinase. 1 hit. SSF88697. PUA-like. 1 hit.
TIGRFAMs	TIGR01027. proB. 1 hit.
PROSITE	PS00902. GLUTAMATE_5_KINASE. 1 hit. PS50890. PUA. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	982012.

Entry information

Entry name

YHJ3_YEAST

Accession

Primary (citable) accession number: P38690
Secondary accession number(s): D3DKY0, Q66R86

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	February 1, 1995
Last modified:	May 1, 2013
This is version 99 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program