Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

P38687 (SRP68_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 105. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Signal recognition particle subunit SRP68
Alternative name(s):
Signal recognition particle 68 kDa protein homolog
Gene names
Name:SRP68
Ordered Locus Names:YPL243W
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length599 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Signal-recognition-particle (SRP) assembly has a crucial role in targeting secretory proteins to the rough endoplasmic reticulum (ER) membrane. SRP is required for the cotranslational protein translocation for ER import and preferentially recognizes strongly hydrophobic signal sequences. It is involved in targeting the nascent chain-ribosome (RNC) complex to the ER and is proposed to participate in the arrest of nascent chain elongation during membrane targeting. SRP68 binds the 7S RNA, SRP72 binds to this complex subsequently.

Subunit structure

Fungal signal recognition particle (SRP) complex consists of a 7S RNA molecule (scR1) and at least six protein subunits: SRP72, SRP68, SRP54, SEC65, SRP21 and SRP14. Ref.1

Subcellular location

Cytoplasm By similarity. Nucleus Ref.4.

Miscellaneous

Present with 6280 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the SRP68 family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 599599Signal recognition particle subunit SRP68
PRO_0000135232

Sequences

Sequence LengthMass (Da)Tools
P38687 [UniParc].

Last modified February 1, 1995. Version 1.
Checksum: 241749187CE95499

FASTA59969,006
        10         20         30         40         50         60 
MVAYSPIIAT YGNRAEQFLE TDSDFAKYHA KLNKKLQHLR SRCHLVTKDT KKYSSKNKYG 

        70         80         90        100        110        120 
EINSEDYDNK TKLIGVLILL HAERDLALAE TLKLRARQRG KLKKSEEKVL STRLKKACKT 

       130        140        150        160        170        180 
ADKLVNVTQN EQQWITRAQY LAFAKLVHSE YLINGKRFKR KDNAKISNNL ALVFAALEHL 

       190        200        210        220        230        240 
KNLSLLAEEV VDNIVNKYQY SLKQYAGNLI TTPEINNFIV ERVQSDENKD DELVKLLLDN 

       250        260        270        280        290        300 
GFNMKKITTS TEDQKVTTNI NWRSFNAKII DAEVAQFLEQ GLSIHPTQIT QYTQRLSKLE 

       310        320        330        340        350        360 
KALDRHEFFI ANHDDQDDID EMVENSSENN QIILAYIKYN ILLTSISRER DLFTHLWNQW 

       370        380        390        400        410        420 
LKLNTSLPSK LTKYKEMERI VKNLTKYLSD IMELPGVYSD DELLSQLDLC KLYFQLFLNT 

       430        440        450        460        470        480 
GCLSVLYQSK GRYMEALALY VDAYRRLENK LSEIESLDEI LLPANLLSLN SVRSLQKRIE 

       490        500        510        520        530        540 
NGGNSVITLA EYEKRNHGGS LGKYDLTVIE KLDSKKILPT DIQLKNLFPL KPKMLPIPSK 

       550        560        570        580        590 
PTLFDLAFNY ITYDKQEPSA SQVKDSVTET ESISQTPISN EQTEGEPKKK RGFLGLFGR 

« Hide

References

« Hide 'large scale' references
[1]"Subunits of the Saccharomyces cerevisiae signal recognition particle required for its functional expression."
Brown J.D., Hann B.C., Medzihradszky K.F., Niwa M., Burlingame A.L., Walter P.
EMBO J. 13:4390-4400(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PARTIAL PROTEIN SEQUENCE, IDENTIFICATION IN THE SRP COMPLEX.
Strain: ATCC 204508 / S288c.
[2]"The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI."
Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W., Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D., Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E., Churcher C.M. expand/collapse author list , Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H., DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N., Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K., Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M., Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A., Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S., Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D., Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S., Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B., Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A., Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R., Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A., Vo D.H., Hani J.
Nature 387:103-105(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]"The reference genome sequence of Saccharomyces cerevisiae: Then and now."
Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.
G3 (Bethesda) 4:389-398(2014) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[4]"Biogenesis of the signal recognition particle (SRP) involves import of SRP proteins into the nucleolus, assembly with the SRP-RNA, and Xpo1p-mediated export."
Grosshans H., Deinert K., Hurt E.C., Simos G.
J. Cell Biol. 153:745-762(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: ASSEMBLY OF THE SRP COMPLEX, SUBCELLULAR LOCATION.
[5]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[6]"Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[7]"N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB."
Van Damme P., Lasa M., Polevoda B., Gazquez C., Elosegui-Artola A., Kim D.S., De Juan-Pardo E., Demeyer K., Hole K., Larrea E., Timmerman E., Prieto J., Arnesen T., Sherman F., Gevaert K., Aldabe R.
Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
L35177 Genomic DNA. Translation: AAA53401.1.
Z67751 Genomic DNA. Translation: CAA91601.1.
Z73599 Genomic DNA. Translation: CAA97964.1.
BK006949 Genomic DNA. Translation: DAA11194.1.
PIRS47928.
RefSeqNP_015081.1. NM_001184057.1.

3D structure databases

ProteinModelPortalP38687.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid35920. 23 interactions.
DIPDIP-6742N.
IntActP38687. 18 interactions.
MINTMINT-626446.
STRING4932.YPL243W.

Proteomic databases

MaxQBP38687.
PaxDbP38687.
PeptideAtlasP38687.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYPL243W; YPL243W; YPL243W.
GeneID855833.
KEGGsce:YPL243W.

Organism-specific databases

CYGDYPL243w.
SGDS000006164. SRP68.

Phylogenomic databases

eggNOGNOG321930.
HOGENOMHOG000141896.
KOK03107.
OMALPGVYND.
OrthoDBEOG79PJZ9.

Enzyme and pathway databases

BioCycYEAST:G3O-34129-MONOMER.

Gene expression databases

GenevestigatorP38687.

Family and domain databases

ProtoNetSearch...

Other

NextBio980398.
PROP38687.

Entry information

Entry nameSRP68_YEAST
AccessionPrimary (citable) accession number: P38687
Secondary accession number(s): D6W3C8
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: May 14, 2014
This is version 105 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome XVI

Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families