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P38687

- SRP68_YEAST

UniProt

P38687 - SRP68_YEAST

Protein

Signal recognition particle subunit SRP68

Gene

SRP68

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 106 (01 Oct 2014)
      Sequence version 1 (01 Feb 1995)
      Previous versions | rss
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    Functioni

    Signal-recognition-particle (SRP) assembly has a crucial role in targeting secretory proteins to the rough endoplasmic reticulum (ER) membrane. SRP is required for the cotranslational protein translocation for ER import and preferentially recognizes strongly hydrophobic signal sequences. It is involved in targeting the nascent chain-ribosome (RNC) complex to the ER and is proposed to participate in the arrest of nascent chain elongation during membrane targeting. SRP68 binds the 7S RNA, SRP72 binds to this complex subsequently.

    GO - Molecular functioni

    1. RNA binding Source: UniProtKB-KW
    2. signal sequence binding Source: SGD

    GO - Biological processi

    1. protein targeting to ER Source: SGD
    2. SRP-dependent cotranslational protein targeting to membrane, signal sequence recognition Source: SGD

    Keywords - Molecular functioni

    Ribonucleoprotein

    Keywords - Ligandi

    RNA-binding

    Enzyme and pathway databases

    BioCyciYEAST:G3O-34129-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Signal recognition particle subunit SRP68
    Alternative name(s):
    Signal recognition particle 68 kDa protein homolog
    Gene namesi
    Name:SRP68
    Ordered Locus Names:YPL243W
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XVI

    Organism-specific databases

    CYGDiYPL243w.
    SGDiS000006164. SRP68.

    Subcellular locationi

    Cytoplasm By similarity. Nucleus 1 Publication

    GO - Cellular componenti

    1. nucleus Source: UniProtKB-SubCell
    2. signal recognition particle, endoplasmic reticulum targeting Source: SGD

    Keywords - Cellular componenti

    Cytoplasm, Nucleus, Signal recognition particle

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 599599Signal recognition particle subunit SRP68PRO_0000135232Add
    BLAST

    Proteomic databases

    MaxQBiP38687.
    PaxDbiP38687.
    PeptideAtlasiP38687.

    Expressioni

    Gene expression databases

    GenevestigatoriP38687.

    Interactioni

    Subunit structurei

    Fungal signal recognition particle (SRP) complex consists of a 7S RNA molecule (scR1) and at least six protein subunits: SRP72, SRP68, SRP54, SEC65, SRP21 and SRP14.1 Publication

    Protein-protein interaction databases

    BioGridi35920. 23 interactions.
    DIPiDIP-6742N.
    IntActiP38687. 18 interactions.
    MINTiMINT-626446.
    STRINGi4932.YPL243W.

    Structurei

    3D structure databases

    ProteinModelPortaliP38687.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the SRP68 family.Curated

    Phylogenomic databases

    eggNOGiNOG321930.
    HOGENOMiHOG000141896.
    KOiK03107.
    OMAiLPGVYND.
    OrthoDBiEOG79PJZ9.

    Sequencei

    Sequence statusi: Complete.

    P38687-1 [UniParc]FASTAAdd to Basket

    « Hide

    MVAYSPIIAT YGNRAEQFLE TDSDFAKYHA KLNKKLQHLR SRCHLVTKDT    50
    KKYSSKNKYG EINSEDYDNK TKLIGVLILL HAERDLALAE TLKLRARQRG 100
    KLKKSEEKVL STRLKKACKT ADKLVNVTQN EQQWITRAQY LAFAKLVHSE 150
    YLINGKRFKR KDNAKISNNL ALVFAALEHL KNLSLLAEEV VDNIVNKYQY 200
    SLKQYAGNLI TTPEINNFIV ERVQSDENKD DELVKLLLDN GFNMKKITTS 250
    TEDQKVTTNI NWRSFNAKII DAEVAQFLEQ GLSIHPTQIT QYTQRLSKLE 300
    KALDRHEFFI ANHDDQDDID EMVENSSENN QIILAYIKYN ILLTSISRER 350
    DLFTHLWNQW LKLNTSLPSK LTKYKEMERI VKNLTKYLSD IMELPGVYSD 400
    DELLSQLDLC KLYFQLFLNT GCLSVLYQSK GRYMEALALY VDAYRRLENK 450
    LSEIESLDEI LLPANLLSLN SVRSLQKRIE NGGNSVITLA EYEKRNHGGS 500
    LGKYDLTVIE KLDSKKILPT DIQLKNLFPL KPKMLPIPSK PTLFDLAFNY 550
    ITYDKQEPSA SQVKDSVTET ESISQTPISN EQTEGEPKKK RGFLGLFGR 599
    Length:599
    Mass (Da):69,006
    Last modified:February 1, 1995 - v1
    Checksum:i241749187CE95499
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L35177 Genomic DNA. Translation: AAA53401.1.
    Z67751 Genomic DNA. Translation: CAA91601.1.
    Z73599 Genomic DNA. Translation: CAA97964.1.
    BK006949 Genomic DNA. Translation: DAA11194.1.
    PIRiS47928.
    RefSeqiNP_015081.1. NM_001184057.1.

    Genome annotation databases

    EnsemblFungiiYPL243W; YPL243W; YPL243W.
    GeneIDi855833.
    KEGGisce:YPL243W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L35177 Genomic DNA. Translation: AAA53401.1 .
    Z67751 Genomic DNA. Translation: CAA91601.1 .
    Z73599 Genomic DNA. Translation: CAA97964.1 .
    BK006949 Genomic DNA. Translation: DAA11194.1 .
    PIRi S47928.
    RefSeqi NP_015081.1. NM_001184057.1.

    3D structure databases

    ProteinModelPortali P38687.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 35920. 23 interactions.
    DIPi DIP-6742N.
    IntActi P38687. 18 interactions.
    MINTi MINT-626446.
    STRINGi 4932.YPL243W.

    Proteomic databases

    MaxQBi P38687.
    PaxDbi P38687.
    PeptideAtlasi P38687.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YPL243W ; YPL243W ; YPL243W .
    GeneIDi 855833.
    KEGGi sce:YPL243W.

    Organism-specific databases

    CYGDi YPL243w.
    SGDi S000006164. SRP68.

    Phylogenomic databases

    eggNOGi NOG321930.
    HOGENOMi HOG000141896.
    KOi K03107.
    OMAi LPGVYND.
    OrthoDBi EOG79PJZ9.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-34129-MONOMER.

    Miscellaneous databases

    NextBioi 980398.
    PROi P38687.

    Gene expression databases

    Genevestigatori P38687.

    Family and domain databases

    ProtoNeti Search...

    Publicationsi

    1. "Subunits of the Saccharomyces cerevisiae signal recognition particle required for its functional expression."
      Brown J.D., Hann B.C., Medzihradszky K.F., Niwa M., Burlingame A.L., Walter P.
      EMBO J. 13:4390-4400(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PARTIAL PROTEIN SEQUENCE, IDENTIFICATION IN THE SRP COMPLEX.
      Strain: ATCC 204508 / S288c.
    2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI."
      Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W., Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D., Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E., Churcher C.M.
      , Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H., DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N., Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K., Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M., Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A., Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S., Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D., Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S., Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B., Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A., Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R., Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A., Vo D.H., Hani J.
      Nature 387:103-105(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    4. "Biogenesis of the signal recognition particle (SRP) involves import of SRP proteins into the nucleolus, assembly with the SRP-RNA, and Xpo1p-mediated export."
      Grosshans H., Deinert K., Hurt E.C., Simos G.
      J. Cell Biol. 153:745-762(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: ASSEMBLY OF THE SRP COMPLEX, SUBCELLULAR LOCATION.
    5. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    6. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
      Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
      Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    7. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiSRP68_YEAST
    AccessioniPrimary (citable) accession number: P38687
    Secondary accession number(s): D6W3C8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1995
    Last sequence update: February 1, 1995
    Last modified: October 1, 2014
    This is version 106 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 6280 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome XVI
      Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names

    External Data

    Dasty 3