TorCAD operon transcriptional regulatory protein TorR

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P38684 (TORR_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 118. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
TorCAD operon transcriptional regulatory protein TorR

Gene names

Name:	torR
Ordered Locus Names:	b0995, JW0980

Organism

Escherichia coli (strain K12) [Reference proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia ›

Protein attributes

Sequence length	230 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Member of the two-component regulatory system TorS/TorR involved in the anaerobic utilization of trimethylamine-N-oxide (TMAO). Phosphorylated TorR activates the transcription of the torCAD operon by binding to four decameric boxes located in the torCAD promoter. Box1, 2 and 4 contain the DNA sequence 5'-CTGTTCATAT-3' and box3 contains the DNA sequence 5'-CCGTTCATCC-3'. Phosphorylated as well as unphosphorylated TorR negatively regulates its own expression by binding to box1 and 2.
Subunit structure	Interacts with TorI. TorI binds to the effector domain of TorR. This interaction, which does not interfere with TorR DNA binding activity, probably prevents the recruitment of RNA polymerase to the torCAD promoter. Ref.9
Subcellular location	Cytoplasm Probable.
Post-translational modification	Phosphorylated and dephosphorylated by TorS.
Sequence similarities	Contains 1 response regulatory domain.

Ontologies

Keywords
Biological process	Transcription Transcription regulation Two-component regulatory system
Cellular component	Cytoplasm
Ligand	DNA-binding
Molecular function	Activator
PTM	Phosphoprotein
Technical term	3D-structure Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	regulation of transcription, DNA-dependent Inferred from direct assay Ref.1. Source: EcoCyc transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	DNA binding Inferred from electronic annotation. Source: UniProtKB-KW phosphorelay response regulator activity Inferred from direct assay Ref.1. Source: EcoCyc
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 230

230

TorCAD operon transcriptional regulatory protein TorR

PRO_0000081255

Regions

Domain

1 – 117

117

Response regulatory

Amino acid modifications

Modified residue

4-aspartylphosphate Probable

Experimental info

Mutagenesis

D → A: Loss of phosphorylation. Ref.7

Sequence conflict

134

C → L in CAA63922. Ref.1

Secondary structure

230

Helix Strand Turn

Details...

Sequences

Sequence

Length

Mass (Da)

Tools

P38684 [UniParc].

Last modified November 1, 1997. Version 2.
Checksum: D1936404503FBA09
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FASTA

230

26,233

        10         20         30         40         50         60 
MPHHIVIVED EPVTQARLQS YFTQEGYTVS VTASGAGLRE IMQNQSVDLI LLDINLPDEN 

        70         80         90        100        110        120 
GLMLTRALRE RSTVGIILVT GRSDRIDRIV GLEMGADDYV TKPLELRELV VRVKNLLWRI 

       130        140        150        160        170        180 
DLARQAQPHT QDNCYRFAGY CLNVSRHTLE RDGEPIKLTR AEYEMLVAFV TNPGEILSRE 

       190        200        210        220        230 
RLLRMLSARR VENPDLRTVD VLIRRLRHKL SADLLVTQHG EGYFLAADVC

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The torR gene of Escherichia coli encodes a response regulator protein involved in the expression of the trimethylamine N-oxide reductase genes." Simon G., Mejean V., Jourlin C., Chippaux M., Pascal M.-C. J. Bacteriol. 176:5601-5606(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-7. Strain: K12 / MC4100 / ATCC 35695 / DSM 6574.
[2]	Erratum Simon G., Mejean V., Jourlin C., Chippaux M., Pascal M.-C. J. Bacteriol. 177:275-275(1995) [PubMed] [Europe PMC] [Abstract]
[3]	"A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 12.7-28.0 min region on the linkage map." Oshima T., Aiba H., Baba T., Fujita K., Hayashi K., Honjo A., Ikemoto K., Inada T., Itoh T., Kajihara M., Kanai K., Kashimoto K., Kimura S., Kitagawa M., Makino K., Masuda S., Miki T., Mizobuchi K. Horiuchi T. DNA Res. 3:137-155(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[4]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[5]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[6]	"Binding of the TorR regulator to cis-acting direct repeats activates tor operon expression." Simon G., Jourlin C., Ansaldi M., Pascal M.-C., Chippaux M., Mejean V. Mol. Microbiol. 17:971-980(1995) [PubMed] [Europe PMC] [Abstract] Cited for: CHARACTERIZATION. Strain: K12 / MC4100 / ATCC 35695 / DSM 6574.
[7]	"Transphosphorylation of the TorR response regulator requires the three phosphorylation sites of the TorS unorthodox sensor in Escherichia coli." Jourlin C., Ansaldi M., Mejean V. J. Mol. Biol. 267:770-777(1997) [PubMed] [Europe PMC] [Abstract] Cited for: MUTAGENESIS OF ASP-53.
[8]	"The TorR high-affinity binding site plays a key role in both torR autoregulation and torCAD operon expression in Escherichia coli." Ansaldi M., Simon G., Lepelletier M., Mejean V. J. Bacteriol. 182:961-966(2000) [PubMed] [Europe PMC] [Abstract] Cited for: CHARACTERIZATION. Strain: K12 / MC4100 / ATCC 35695 / DSM 6574.
[9]	"TorI, a response regulator inhibitor of phage origin in Escherichia coli." Ansaldi M., Theraulaz L., Mejean V. Proc. Natl. Acad. Sci. U.S.A. 101:9423-9428(2004) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH TORI.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

X94231 Genomic DNA. Translation: CAA63922.1.
U00096 Genomic DNA. Translation: AAC74080.1.
AP009048 Genomic DNA. Translation: BAA36137.1.

PIR

A64841.

RefSeq

NP_415515.1. NC_000913.2.
YP_489268.1. NC_007779.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1ZGZ	X-ray	1.80	A/B/C/D	1-121	[»]

ProteinModelPortal

P38684.

SMR

P38684. Positions 2-225.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-11016N.

IntAct

P38684. 3 interactions.

MINT

MINT-1283676.

STRING

511145.b0995.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblBacteria

AAC74080; AAC74080; b0995.
BAA36137; BAA36137; BAA36137.

GeneID

12932494.
946182.

KEGG

ecj:Y75_p0968.
eco:b0995.

PATRIC

32117215. VBIEscCol129921_1031.

Organism-specific databases

EchoBASE

EB2499.

EcoGene

EG12615. torR.

Phylogenomic databases

eggNOG

COG0745.

HOGENOM

HOG000034819.

K07772.

OMA

YCYRFAG.

ProtClustDB

PRK10766.

Enzyme and pathway databases

BioCyc

EcoCyc:TORR-MONOMER.
ECOL316407:JW0980-MONOMER.

Gene expression databases

Genevestigator

P38684.

Family and domain databases

Gene3D

1.10.10.10. 1 hit.

InterPro

IPR011006. CheY-like_superfamily.
IPR001867. Sig_transdc_resp-reg_C.
IPR001789. Sig_transdc_resp-reg_receiver.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]

Pfam

PF00072. Response_reg. 1 hit.
PF00486. Trans_reg_C. 1 hit.
[Graphical view]

SMART

SM00448. REC. 1 hit.
SM00862. Trans_reg_C. 1 hit.
[Graphical view]

SUPFAM

SSF52172. CheY_like. 1 hit.

PROSITE

PS50110. RESPONSE_REGULATORY. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

P38684.

Entry information

Entry name

TORR_ECOLI

Accession

Primary (citable) accession number: P38684
Secondary accession number(s): P77344

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	November 1, 1997
Last modified:	May 1, 2013
This is version 118 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program

Relevant documents

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Experimental info

Secondary structure

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents