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Protein

TorCAD operon transcriptional regulatory protein TorR

Gene

torR

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Member of the two-component regulatory system TorS/TorR involved in the anaerobic utilization of trimethylamine-N-oxide (TMAO). Phosphorylated TorR activates the transcription of the torCAD operon by binding to four decameric boxes located in the torCAD promoter. Box1, 2 and 4 contain the DNA sequence 5'-CTGTTCATAT-3' and box3 contains the DNA sequence 5'-CCGTTCATCC-3'. Phosphorylated as well as unphosphorylated TorR negatively regulates its own expression by binding to box1 and 2.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi132 – 227OmpR/PhoB-typePROSITE-ProRule annotationAdd BLAST96

GO - Molecular functioni

  • DNA binding Source: UniProtKB-KW
  • phosphorelay response regulator activity Source: EcoCyc

GO - Biological processi

  • regulation of transcription, DNA-templated Source: EcoCyc
  • transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Transcription, Transcription regulation, Two-component regulatory system

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

BioCyciEcoCyc:TORR-MONOMER.
ECOL316407:JW0980-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
TorCAD operon transcriptional regulatory protein TorR
Gene namesi
Name:torR
Ordered Locus Names:b0995, JW0980
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG12615. torR.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi53D → A: Loss of phosphorylation. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000812551 – 230TorCAD operon transcriptional regulatory protein TorRAdd BLAST230

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei534-aspartylphosphateCurated1

Post-translational modificationi

Phosphorylated and dephosphorylated by TorS.

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP38684.
PRIDEiP38684.

Interactioni

Subunit structurei

Interacts with TorI. TorI binds to the effector domain of TorR. This interaction, which does not interfere with TorR DNA binding activity, probably prevents the recruitment of RNA polymerase to the torCAD promoter.1 Publication

Protein-protein interaction databases

BioGridi4261488. 9 interactors.
DIPiDIP-11016N.
IntActiP38684. 4 interactors.
MINTiMINT-1283676.
STRINGi511145.b0995.

Structurei

Secondary structure

1230
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi4 – 8Combined sources5
Helixi12 – 24Combined sources13
Beta strandi28 – 34Combined sources7
Helixi35 – 44Combined sources10
Beta strandi48 – 54Combined sources7
Beta strandi57 – 59Combined sources3
Helixi61 – 69Combined sources9
Beta strandi75 – 82Combined sources8
Helixi85 – 94Combined sources10
Beta strandi97 – 103Combined sources7
Helixi106 – 120Combined sources15

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1ZGZX-ray1.80A/B/C/D1-122[»]
ProteinModelPortaliP38684.
SMRiP38684.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP38684.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini4 – 117Response regulatoryPROSITE-ProRule annotationAdd BLAST114

Sequence similaritiesi

Contains 1 OmpR/PhoB-type DNA-binding domain.PROSITE-ProRule annotation
Contains 1 response regulatory domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG4106WF9. Bacteria.
COG0745. LUCA.
HOGENOMiHOG000034819.
InParanoidiP38684.
KOiK07772.
OMAiREVWGWD.
PhylomeDBiP38684.

Family and domain databases

CDDicd00383. trans_reg_C. 1 hit.
Gene3Di1.10.10.10. 1 hit.
InterProiIPR011006. CheY-like_superfamily.
IPR001867. OmpR/PhoB-type_DNA-bd.
IPR001789. Sig_transdc_resp-reg_receiver.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF00072. Response_reg. 1 hit.
PF00486. Trans_reg_C. 1 hit.
[Graphical view]
SMARTiSM00448. REC. 1 hit.
SM00862. Trans_reg_C. 1 hit.
[Graphical view]
SUPFAMiSSF52172. SSF52172. 1 hit.
PROSITEiPS51755. OMPR_PHOB. 1 hit.
PS50110. RESPONSE_REGULATORY. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P38684-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPHHIVIVED EPVTQARLQS YFTQEGYTVS VTASGAGLRE IMQNQSVDLI
60 70 80 90 100
LLDINLPDEN GLMLTRALRE RSTVGIILVT GRSDRIDRIV GLEMGADDYV
110 120 130 140 150
TKPLELRELV VRVKNLLWRI DLARQAQPHT QDNCYRFAGY CLNVSRHTLE
160 170 180 190 200
RDGEPIKLTR AEYEMLVAFV TNPGEILSRE RLLRMLSARR VENPDLRTVD
210 220 230
VLIRRLRHKL SADLLVTQHG EGYFLAADVC
Length:230
Mass (Da):26,233
Last modified:November 1, 1997 - v2
Checksum:iD1936404503FBA09
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti134C → L in CAA63922 (PubMed:8083154).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X94231 Genomic DNA. Translation: CAA63922.1.
U00096 Genomic DNA. Translation: AAC74080.1.
AP009048 Genomic DNA. Translation: BAA36137.1.
PIRiA64841.
RefSeqiNP_415515.1. NC_000913.3.
WP_001120125.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC74080; AAC74080; b0995.
BAA36137; BAA36137; BAA36137.
GeneIDi946182.
KEGGiecj:JW0980.
eco:b0995.
PATRICi32117215. VBIEscCol129921_1031.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X94231 Genomic DNA. Translation: CAA63922.1.
U00096 Genomic DNA. Translation: AAC74080.1.
AP009048 Genomic DNA. Translation: BAA36137.1.
PIRiA64841.
RefSeqiNP_415515.1. NC_000913.3.
WP_001120125.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1ZGZX-ray1.80A/B/C/D1-122[»]
ProteinModelPortaliP38684.
SMRiP38684.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4261488. 9 interactors.
DIPiDIP-11016N.
IntActiP38684. 4 interactors.
MINTiMINT-1283676.
STRINGi511145.b0995.

Proteomic databases

PaxDbiP38684.
PRIDEiP38684.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC74080; AAC74080; b0995.
BAA36137; BAA36137; BAA36137.
GeneIDi946182.
KEGGiecj:JW0980.
eco:b0995.
PATRICi32117215. VBIEscCol129921_1031.

Organism-specific databases

EchoBASEiEB2499.
EcoGeneiEG12615. torR.

Phylogenomic databases

eggNOGiENOG4106WF9. Bacteria.
COG0745. LUCA.
HOGENOMiHOG000034819.
InParanoidiP38684.
KOiK07772.
OMAiREVWGWD.
PhylomeDBiP38684.

Enzyme and pathway databases

BioCyciEcoCyc:TORR-MONOMER.
ECOL316407:JW0980-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP38684.
PROiP38684.

Family and domain databases

CDDicd00383. trans_reg_C. 1 hit.
Gene3Di1.10.10.10. 1 hit.
InterProiIPR011006. CheY-like_superfamily.
IPR001867. OmpR/PhoB-type_DNA-bd.
IPR001789. Sig_transdc_resp-reg_receiver.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF00072. Response_reg. 1 hit.
PF00486. Trans_reg_C. 1 hit.
[Graphical view]
SMARTiSM00448. REC. 1 hit.
SM00862. Trans_reg_C. 1 hit.
[Graphical view]
SUPFAMiSSF52172. SSF52172. 1 hit.
PROSITEiPS51755. OMPR_PHOB. 1 hit.
PS50110. RESPONSE_REGULATORY. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTORR_ECOLI
AccessioniPrimary (citable) accession number: P38684
Secondary accession number(s): P77344
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: November 1, 1997
Last modified: November 2, 2016
This is version 142 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.