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P38684

- TORR_ECOLI

UniProt

P38684 - TORR_ECOLI

Protein

TorCAD operon transcriptional regulatory protein TorR

Gene

torR

Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 129 (01 Oct 2014)
      Sequence version 2 (01 Nov 1997)
      Previous versions | rss
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    Functioni

    Member of the two-component regulatory system TorS/TorR involved in the anaerobic utilization of trimethylamine-N-oxide (TMAO). Phosphorylated TorR activates the transcription of the torCAD operon by binding to four decameric boxes located in the torCAD promoter. Box1, 2 and 4 contain the DNA sequence 5'-CTGTTCATAT-3' and box3 contains the DNA sequence 5'-CCGTTCATCC-3'. Phosphorylated as well as unphosphorylated TorR negatively regulates its own expression by binding to box1 and 2.

    GO - Molecular functioni

    1. DNA binding Source: UniProtKB-KW
    2. phosphorelay response regulator activity Source: EcoCyc

    GO - Biological processi

    1. intracellular signal transduction Source: GOC
    2. regulation of transcription, DNA-templated Source: EcoCyc
    3. transcription, DNA-templated Source: UniProtKB-KW

    Keywords - Molecular functioni

    Activator

    Keywords - Biological processi

    Transcription, Transcription regulation, Two-component regulatory system

    Keywords - Ligandi

    DNA-binding

    Enzyme and pathway databases

    BioCyciEcoCyc:TORR-MONOMER.
    ECOL316407:JW0980-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    TorCAD operon transcriptional regulatory protein TorR
    Gene namesi
    Name:torR
    Ordered Locus Names:b0995, JW0980
    OrganismiEscherichia coli (strain K12)
    Taxonomic identifieri83333 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
    ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

    Organism-specific databases

    EcoGeneiEG12615. torR.

    Subcellular locationi

    Cytoplasm Curated

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi53 – 531D → A: Loss of phosphorylation. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 230230TorCAD operon transcriptional regulatory protein TorRPRO_0000081255Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei53 – 5314-aspartylphosphateCurated

    Post-translational modificationi

    Phosphorylated and dephosphorylated by TorS.

    Keywords - PTMi

    Phosphoprotein

    Expressioni

    Gene expression databases

    GenevestigatoriP38684.

    Interactioni

    Subunit structurei

    Interacts with TorI. TorI binds to the effector domain of TorR. This interaction, which does not interfere with TorR DNA binding activity, probably prevents the recruitment of RNA polymerase to the torCAD promoter.1 Publication

    Protein-protein interaction databases

    DIPiDIP-11016N.
    IntActiP38684. 4 interactions.
    MINTiMINT-1283676.
    STRINGi511145.b0995.

    Structurei

    Secondary structure

    1
    230
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi4 – 85
    Helixi12 – 2413
    Beta strandi28 – 347
    Helixi35 – 4410
    Beta strandi48 – 547
    Beta strandi57 – 593
    Helixi61 – 699
    Beta strandi75 – 828
    Helixi85 – 9410
    Beta strandi97 – 1037
    Helixi106 – 12015

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1ZGZX-ray1.80A/B/C/D1-122[»]
    ProteinModelPortaliP38684.
    SMRiP38684. Positions 2-225.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP38684.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini1 – 117117Response regulatoryPROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 1 response regulatory domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG0745.
    HOGENOMiHOG000034819.
    KOiK07772.
    OMAiFERQHID.
    OrthoDBiEOG6G4VQG.
    PhylomeDBiP38684.

    Family and domain databases

    Gene3Di1.10.10.10. 1 hit.
    InterProiIPR011006. CheY-like_superfamily.
    IPR001867. Sig_transdc_resp-reg_C.
    IPR001789. Sig_transdc_resp-reg_receiver.
    IPR011991. WHTH_DNA-bd_dom.
    [Graphical view]
    PfamiPF00072. Response_reg. 1 hit.
    PF00486. Trans_reg_C. 1 hit.
    [Graphical view]
    SMARTiSM00448. REC. 1 hit.
    SM00862. Trans_reg_C. 1 hit.
    [Graphical view]
    SUPFAMiSSF52172. SSF52172. 1 hit.
    PROSITEiPS50110. RESPONSE_REGULATORY. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P38684-1 [UniParc]FASTAAdd to Basket

    « Hide

    MPHHIVIVED EPVTQARLQS YFTQEGYTVS VTASGAGLRE IMQNQSVDLI    50
    LLDINLPDEN GLMLTRALRE RSTVGIILVT GRSDRIDRIV GLEMGADDYV 100
    TKPLELRELV VRVKNLLWRI DLARQAQPHT QDNCYRFAGY CLNVSRHTLE 150
    RDGEPIKLTR AEYEMLVAFV TNPGEILSRE RLLRMLSARR VENPDLRTVD 200
    VLIRRLRHKL SADLLVTQHG EGYFLAADVC 230
    Length:230
    Mass (Da):26,233
    Last modified:November 1, 1997 - v2
    Checksum:iD1936404503FBA09
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti134 – 1341C → L in CAA63922. (PubMed:8083154)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X94231 Genomic DNA. Translation: CAA63922.1.
    U00096 Genomic DNA. Translation: AAC74080.1.
    AP009048 Genomic DNA. Translation: BAA36137.1.
    PIRiA64841.
    RefSeqiNP_415515.1. NC_000913.3.
    YP_489268.1. NC_007779.1.

    Genome annotation databases

    EnsemblBacteriaiAAC74080; AAC74080; b0995.
    BAA36137; BAA36137; BAA36137.
    GeneIDi12932494.
    946182.
    KEGGiecj:Y75_p0968.
    eco:b0995.
    PATRICi32117215. VBIEscCol129921_1031.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X94231 Genomic DNA. Translation: CAA63922.1 .
    U00096 Genomic DNA. Translation: AAC74080.1 .
    AP009048 Genomic DNA. Translation: BAA36137.1 .
    PIRi A64841.
    RefSeqi NP_415515.1. NC_000913.3.
    YP_489268.1. NC_007779.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1ZGZ X-ray 1.80 A/B/C/D 1-122 [» ]
    ProteinModelPortali P38684.
    SMRi P38684. Positions 2-225.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    DIPi DIP-11016N.
    IntActi P38684. 4 interactions.
    MINTi MINT-1283676.
    STRINGi 511145.b0995.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAC74080 ; AAC74080 ; b0995 .
    BAA36137 ; BAA36137 ; BAA36137 .
    GeneIDi 12932494.
    946182.
    KEGGi ecj:Y75_p0968.
    eco:b0995.
    PATRICi 32117215. VBIEscCol129921_1031.

    Organism-specific databases

    EchoBASEi EB2499.
    EcoGenei EG12615. torR.

    Phylogenomic databases

    eggNOGi COG0745.
    HOGENOMi HOG000034819.
    KOi K07772.
    OMAi FERQHID.
    OrthoDBi EOG6G4VQG.
    PhylomeDBi P38684.

    Enzyme and pathway databases

    BioCyci EcoCyc:TORR-MONOMER.
    ECOL316407:JW0980-MONOMER.

    Miscellaneous databases

    EvolutionaryTracei P38684.
    PROi P38684.

    Gene expression databases

    Genevestigatori P38684.

    Family and domain databases

    Gene3Di 1.10.10.10. 1 hit.
    InterProi IPR011006. CheY-like_superfamily.
    IPR001867. Sig_transdc_resp-reg_C.
    IPR001789. Sig_transdc_resp-reg_receiver.
    IPR011991. WHTH_DNA-bd_dom.
    [Graphical view ]
    Pfami PF00072. Response_reg. 1 hit.
    PF00486. Trans_reg_C. 1 hit.
    [Graphical view ]
    SMARTi SM00448. REC. 1 hit.
    SM00862. Trans_reg_C. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52172. SSF52172. 1 hit.
    PROSITEi PS50110. RESPONSE_REGULATORY. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The torR gene of Escherichia coli encodes a response regulator protein involved in the expression of the trimethylamine N-oxide reductase genes."
      Simon G., Mejean V., Jourlin C., Chippaux M., Pascal M.-C.
      J. Bacteriol. 176:5601-5606(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-7.
      Strain: K12 / MC4100 / ATCC 35695 / DSM 6574.
    2. Erratum
      Simon G., Mejean V., Jourlin C., Chippaux M., Pascal M.-C.
      J. Bacteriol. 177:275-275(1995) [PubMed] [Europe PMC] [Abstract]
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
    4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / MG1655 / ATCC 47076.
    5. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
      Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
      Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
    6. "Binding of the TorR regulator to cis-acting direct repeats activates tor operon expression."
      Simon G., Jourlin C., Ansaldi M., Pascal M.-C., Chippaux M., Mejean V.
      Mol. Microbiol. 17:971-980(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: CHARACTERIZATION.
      Strain: K12 / MC4100 / ATCC 35695 / DSM 6574.
    7. "Transphosphorylation of the TorR response regulator requires the three phosphorylation sites of the TorS unorthodox sensor in Escherichia coli."
      Jourlin C., Ansaldi M., Mejean V.
      J. Mol. Biol. 267:770-777(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: MUTAGENESIS OF ASP-53.
    8. "The TorR high-affinity binding site plays a key role in both torR autoregulation and torCAD operon expression in Escherichia coli."
      Ansaldi M., Simon G., Lepelletier M., Mejean V.
      J. Bacteriol. 182:961-966(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: CHARACTERIZATION.
      Strain: K12 / MC4100 / ATCC 35695 / DSM 6574.
    9. "TorI, a response regulator inhibitor of phage origin in Escherichia coli."
      Ansaldi M., Theraulaz L., Mejean V.
      Proc. Natl. Acad. Sci. U.S.A. 101:9423-9428(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH TORI.

    Entry informationi

    Entry nameiTORR_ECOLI
    AccessioniPrimary (citable) accession number: P38684
    Secondary accession number(s): P77344
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1995
    Last sequence update: November 1, 1997
    Last modified: October 1, 2014
    This is version 129 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Escherichia coli
      Escherichia coli (strain K12): entries and cross-references to EcoGene
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3