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Protein

TorCAD operon transcriptional regulatory protein TorR

Gene

torR

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Member of the two-component regulatory system TorS/TorR involved in the anaerobic utilization of trimethylamine-N-oxide (TMAO). Phosphorylated TorR activates the transcription of the torCAD operon by binding to four decameric boxes located in the torCAD promoter. Box1, 2 and 4 contain the DNA sequence 5'-CTGTTCATAT-3' and box3 contains the DNA sequence 5'-CCGTTCATCC-3'. Phosphorylated as well as unphosphorylated TorR negatively regulates its own expression by binding to box1 and 2.

GO - Molecular functioni

  1. DNA binding Source: UniProtKB-KW
  2. phosphorelay response regulator activity Source: EcoCyc

GO - Biological processi

  1. regulation of transcription, DNA-templated Source: EcoCyc
  2. transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Transcription, Transcription regulation, Two-component regulatory system

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

BioCyciEcoCyc:TORR-MONOMER.
ECOL316407:JW0980-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
TorCAD operon transcriptional regulatory protein TorR
Gene namesi
Name:torR
Ordered Locus Names:b0995, JW0980
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

Organism-specific databases

EcoGeneiEG12615. torR.

Subcellular locationi

Cytoplasm Curated

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi53 – 531D → A: Loss of phosphorylation. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 230230TorCAD operon transcriptional regulatory protein TorRPRO_0000081255Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei53 – 5314-aspartylphosphateCurated

Post-translational modificationi

Phosphorylated and dephosphorylated by TorS.

Keywords - PTMi

Phosphoprotein

Expressioni

Gene expression databases

GenevestigatoriP38684.

Interactioni

Subunit structurei

Interacts with TorI. TorI binds to the effector domain of TorR. This interaction, which does not interfere with TorR DNA binding activity, probably prevents the recruitment of RNA polymerase to the torCAD promoter.1 Publication

Protein-protein interaction databases

DIPiDIP-11016N.
IntActiP38684. 4 interactions.
MINTiMINT-1283676.
STRINGi511145.b0995.

Structurei

Secondary structure

1
230
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi4 – 85Combined sources
Helixi12 – 2413Combined sources
Beta strandi28 – 347Combined sources
Helixi35 – 4410Combined sources
Beta strandi48 – 547Combined sources
Beta strandi57 – 593Combined sources
Helixi61 – 699Combined sources
Beta strandi75 – 828Combined sources
Helixi85 – 9410Combined sources
Beta strandi97 – 1037Combined sources
Helixi106 – 12015Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1ZGZX-ray1.80A/B/C/D1-122[»]
ProteinModelPortaliP38684.
SMRiP38684. Positions 2-225.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP38684.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini1 – 117117Response regulatoryPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 response regulatory domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG0745.
HOGENOMiHOG000034819.
InParanoidiP38684.
KOiK07772.
OMAiVWKERYY.
OrthoDBiEOG6G4VQG.
PhylomeDBiP38684.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
InterProiIPR011006. CheY-like_superfamily.
IPR001867. Sig_transdc_resp-reg_C.
IPR001789. Sig_transdc_resp-reg_receiver.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF00072. Response_reg. 1 hit.
PF00486. Trans_reg_C. 1 hit.
[Graphical view]
SMARTiSM00448. REC. 1 hit.
SM00862. Trans_reg_C. 1 hit.
[Graphical view]
SUPFAMiSSF52172. SSF52172. 1 hit.
PROSITEiPS50110. RESPONSE_REGULATORY. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P38684-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPHHIVIVED EPVTQARLQS YFTQEGYTVS VTASGAGLRE IMQNQSVDLI
60 70 80 90 100
LLDINLPDEN GLMLTRALRE RSTVGIILVT GRSDRIDRIV GLEMGADDYV
110 120 130 140 150
TKPLELRELV VRVKNLLWRI DLARQAQPHT QDNCYRFAGY CLNVSRHTLE
160 170 180 190 200
RDGEPIKLTR AEYEMLVAFV TNPGEILSRE RLLRMLSARR VENPDLRTVD
210 220 230
VLIRRLRHKL SADLLVTQHG EGYFLAADVC
Length:230
Mass (Da):26,233
Last modified:November 1, 1997 - v2
Checksum:iD1936404503FBA09
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti134 – 1341C → L in CAA63922 (PubMed:8083154).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X94231 Genomic DNA. Translation: CAA63922.1.
U00096 Genomic DNA. Translation: AAC74080.1.
AP009048 Genomic DNA. Translation: BAA36137.1.
PIRiA64841.
RefSeqiNP_415515.1. NC_000913.3.
YP_489268.1. NC_007779.1.

Genome annotation databases

EnsemblBacteriaiAAC74080; AAC74080; b0995.
BAA36137; BAA36137; BAA36137.
GeneIDi12932494.
946182.
KEGGiecj:Y75_p0968.
eco:b0995.
PATRICi32117215. VBIEscCol129921_1031.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X94231 Genomic DNA. Translation: CAA63922.1.
U00096 Genomic DNA. Translation: AAC74080.1.
AP009048 Genomic DNA. Translation: BAA36137.1.
PIRiA64841.
RefSeqiNP_415515.1. NC_000913.3.
YP_489268.1. NC_007779.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1ZGZX-ray1.80A/B/C/D1-122[»]
ProteinModelPortaliP38684.
SMRiP38684. Positions 2-225.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-11016N.
IntActiP38684. 4 interactions.
MINTiMINT-1283676.
STRINGi511145.b0995.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC74080; AAC74080; b0995.
BAA36137; BAA36137; BAA36137.
GeneIDi12932494.
946182.
KEGGiecj:Y75_p0968.
eco:b0995.
PATRICi32117215. VBIEscCol129921_1031.

Organism-specific databases

EchoBASEiEB2499.
EcoGeneiEG12615. torR.

Phylogenomic databases

eggNOGiCOG0745.
HOGENOMiHOG000034819.
InParanoidiP38684.
KOiK07772.
OMAiVWKERYY.
OrthoDBiEOG6G4VQG.
PhylomeDBiP38684.

Enzyme and pathway databases

BioCyciEcoCyc:TORR-MONOMER.
ECOL316407:JW0980-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP38684.
PROiP38684.

Gene expression databases

GenevestigatoriP38684.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
InterProiIPR011006. CheY-like_superfamily.
IPR001867. Sig_transdc_resp-reg_C.
IPR001789. Sig_transdc_resp-reg_receiver.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF00072. Response_reg. 1 hit.
PF00486. Trans_reg_C. 1 hit.
[Graphical view]
SMARTiSM00448. REC. 1 hit.
SM00862. Trans_reg_C. 1 hit.
[Graphical view]
SUPFAMiSSF52172. SSF52172. 1 hit.
PROSITEiPS50110. RESPONSE_REGULATORY. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The torR gene of Escherichia coli encodes a response regulator protein involved in the expression of the trimethylamine N-oxide reductase genes."
    Simon G., Mejean V., Jourlin C., Chippaux M., Pascal M.-C.
    J. Bacteriol. 176:5601-5606(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-7.
    Strain: K12 / MC4100 / ATCC 35695 / DSM 6574.
  2. Erratum
    Simon G., Mejean V., Jourlin C., Chippaux M., Pascal M.-C.
    J. Bacteriol. 177:275-275(1995) [PubMed] [Europe PMC] [Abstract]
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  5. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  6. "Binding of the TorR regulator to cis-acting direct repeats activates tor operon expression."
    Simon G., Jourlin C., Ansaldi M., Pascal M.-C., Chippaux M., Mejean V.
    Mol. Microbiol. 17:971-980(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION.
    Strain: K12 / MC4100 / ATCC 35695 / DSM 6574.
  7. "Transphosphorylation of the TorR response regulator requires the three phosphorylation sites of the TorS unorthodox sensor in Escherichia coli."
    Jourlin C., Ansaldi M., Mejean V.
    J. Mol. Biol. 267:770-777(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: MUTAGENESIS OF ASP-53.
  8. "The TorR high-affinity binding site plays a key role in both torR autoregulation and torCAD operon expression in Escherichia coli."
    Ansaldi M., Simon G., Lepelletier M., Mejean V.
    J. Bacteriol. 182:961-966(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION.
    Strain: K12 / MC4100 / ATCC 35695 / DSM 6574.
  9. "TorI, a response regulator inhibitor of phage origin in Escherichia coli."
    Ansaldi M., Theraulaz L., Mejean V.
    Proc. Natl. Acad. Sci. U.S.A. 101:9423-9428(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH TORI.

Entry informationi

Entry nameiTORR_ECOLI
AccessioniPrimary (citable) accession number: P38684
Secondary accession number(s): P77344
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: November 1, 1997
Last modified: January 7, 2015
This is version 132 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.