P38681 (NIR_NEUCR) Reviewed, UniProtKB/Swiss-Prot
Last modified
April 3, 2013.
Version 108.
History...
Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize orderNames and origin
| Protein names | Recommended name: Nitrite reductase [NAD(P)H] EC=1.7.1.4 | ||||
| Gene names |
| ||||
| Organism | Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987) [Reference proteome] | ||||
| Taxonomic identifier | 367110 [NCBI] | ||||
| Taxonomic lineage | Eukaryota › Fungi › Dikarya › Ascomycota › Pezizomycotina › Sordariomycetes › Sordariomycetidae › Sordariales › Sordariaceae › Neurospora › ![]() |
Protein attributes
| Sequence length | 1176 AA. |
| Sequence status | Complete. |
| Protein existence | Evidence at transcript level |
General annotation (Comments)
| Catalytic activity | Ammonium hydroxide + 3 NAD(P)+ + H2O = nitrite + 3 NAD(P)H. |
| Cofactor | Binds 1 siroheme per subunit. Binds 1 4Fe-4S cluster per subunit. FAD. Binds 1 2Fe-2S cluster per subunit By similarity. |
| Pathway | |
| Subunit structure | Homodimer By similarity. |
| Induction | By nitrate. |
| Sequence similarities | Belongs to the nitrite and sulfite reductase 4Fe-4S domain family. Contains 1 Rieske domain. |
| Sequence caution | The sequence EAA31119.2 differs from that shown. Reason: Erroneous initiation. |
Ontologies
| Keywords | |
|---|---|
| Biological process | Nitrate assimilation |
| Ligand | 2Fe-2S 4Fe-4S FAD Flavoprotein Heme Iron Iron-sulfur Metal-binding NADP |
| Molecular function | Oxidoreductase |
| Technical term | Complete proteome Reference proteome |
| Gene Ontology (GO) | |
| Biological_process | nitrate assimilation Inferred from electronic annotation. Source: UniProtKB-UniPathway |
| Molecular_function | 2 iron, 2 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW 4 iron, 4 sulfur cluster bindingInferred from electronic annotation. Source: UniProtKB-KW NADP bindingInferred from electronic annotation. Source: InterPro flavin adenine dinucleotide bindingInferred from electronic annotation. Source: InterPro heme bindingInferred from electronic annotation. Source: InterPro metal ion bindingInferred from electronic annotation. Source: UniProtKB-KW nitrite reductase [NAD(P)H] activityInferred from electronic annotation. Source: EC |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 1176 | 1176 | Nitrite reductase [NAD(P)H] | PRO_0000199960 | |||||
Regions | |||||||||
| Domain | 942 – 1094 | 153 | Rieske; atypical | ||||||
| Nucleotide binding | 26 – 60 | 35 | FAD Potential | ||||||
| Nucleotide binding | 183 – 215 | 33 | NAD or NADP Potential | ||||||
| Compositional bias | 998 – 1054 | 57 | Pro/Ser-rich | ||||||
Sites | |||||||||
| Metal binding | 717 | 1 | Iron-sulfur (4Fe-4S) By similarity | ||||||
| Metal binding | 723 | 1 | Iron-sulfur (4Fe-4S) By similarity | ||||||
| Metal binding | 757 | 1 | Iron-sulfur (4Fe-4S) By similarity | ||||||
| Metal binding | 761 | 1 | Iron (siroheme axial ligand) By similarity | ||||||
| Metal binding | 761 | 1 | Iron-sulfur (4Fe-4S) By similarity | ||||||
| Metal binding | 981 | 1 | Iron-sulfur (2Fe-2S) By similarity | ||||||
| Metal binding | 983 | 1 | Iron-sulfur (2Fe-2S); via pros nitrogen By similarity | ||||||
| Metal binding | 1058 | 1 | Iron-sulfur (2Fe-2S) By similarity | ||||||
| Metal binding | 1061 | 1 | Iron-sulfur (2Fe-2S); via pros nitrogen By similarity | ||||||
Experimental info | |||||||||
| Sequence conflict | 357 | 1 | T → S in L07391. Ref.1 | ||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "Molecular cloning, characterization, and nucleotide sequence of nit-6, the structural gene for nitrite reductase in Neurospora crassa." Exley G.E., Colandene J.D., Garrett R.H. J. Bacteriol. 175:2379-2392(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987. |
| [2] | "The genome sequence of the filamentous fungus Neurospora crassa." Galagan J.E., Calvo S.E., Borkovich K.A., Selker E.U., Read N.D., Jaffe D.B., FitzHugh W., Ma L.-J., Smirnov S., Purcell S., Rehman B., Elkins T., Engels R., Wang S., Nielsen C.B., Butler J., Endrizzi M., Qui D. Birren B.W.Nature 422:859-868(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987. |
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | L07391 Genomic DNA. No translation available. AABX02000016 Genomic DNA. Translation: EAA31119.2. Different initiation. |
| PIR | A49848. |
| RefSeq | XP_960355.2. XM_955262.2. |
| UniGene | Ncr.8742. |
3D structure databases | |
| ProteinModelPortal | P38681. |
| ModBase | Search... |
Protein-protein interaction databases | |
| STRING | 5141.NCU04720.1. |
Protocols and materials databases | |
| StructuralBiologyKnowledgebase | Search... |
Genome annotation databases | |
| EnsemblFungi | EFNCRT00000004474; EFNCRP00000004474; EFNCRG00000004468. |
| GeneID | 3876518. |
| KEGG | ncr:NCU04720. |
Phylogenomic databases | |
| eggNOG | COG1251. |
| HOGENOM | HOG000196164. |
| KO | K00362. |
| OrthoDB | EOG4M68RN. |
Enzyme and pathway databases | |
| BioCyc | MetaCyc:MONOMER-12533. |
| UniPathway | UPA00653. |
Family and domain databases | |
| Gene3D | 2.102.10.10. 2 hits. 3.90.480.10. 1 hit. |
| InterPro | IPR007419. BFD-like_2Fe2S-bd_dom. IPR013027. FAD_pyr_nucl-diS_OxRdtase. IPR005117. NiRdtase/SiRdtase_haem-b_fer. IPR012744. Nitri_red_NirB. IPR006067. NO2/SO3_Rdtase_4Fe4S_dom. IPR006066. NO2/SO3_Rdtase_FeS/sirohaem_BS. IPR023753. Pyr_nucl-diS_OxRdtase_FAD/NAD. IPR001327. Pyr_OxRdtase_NAD-bd_dom. IPR017941. Rieske_2Fe-2S. [Graphical view] |
| Pfam | PF04324. Fer2_BFD. 1 hit. PF01077. NIR_SIR. 1 hit. PF03460. NIR_SIR_ferr. 1 hit. PF00070. Pyr_redox. 1 hit. PF07992. Pyr_redox_2. 1 hit. PF00355. Rieske. 1 hit. [Graphical view] |
| PRINTS | PR00368. FADPNR. PR00397. SIROHAEM. |
| SUPFAM | SSF55124. NiR_SiRalpha_1/3. 1 hit. SSF50022. Rieske_dom. 1 hit. |
| TIGRFAMs | TIGR02374. nitri_red_nirB. 1 hit. |
| PROSITE | PS00365. NIR_SIR. 1 hit. PS51296. RIESKE. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Entry information
| Entry name | NIR_NEUCR | ||||||||
| Accession | Primary (citable) accession number: P38681 Secondary accession number(s): Q7RVB5 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation program | Fungal Protein Annotation Program | ||||||||
Relevant documents
| PATHWAY comments Index of metabolic and biosynthesis pathways |
| SIMILARITY comments Index of protein domains and families |

Clusters with
