P38681 (NIR_NEUCR) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Nitrite reductase [NAD(P)H]
EC=1.7.1.4

Gene names

Name:	nit-6
ORF Names:	NCU04720

Organism

Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987) [Reference proteome]

Taxonomic identifier

367110 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Pezizomycotina › leotiomyceta › sordariomyceta › Sordariomycetes › Sordariomycetidae › Sordariales › Sordariaceae › Neurospora › Neurospora crassa

Protein attributes

Sequence length	1176 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Catalytic activity	Ammonium hydroxide + 3 NAD(P)+ + H2O = nitrite + 3 NAD(P)H.
Cofactor	Binds 1 siroheme per subunit. Binds 1 4Fe-4S cluster per subunit. FAD. Binds 1 2Fe-2S cluster per subunit By similarity.
Pathway	Nitrogen metabolism; nitrate reduction (assimilation).
Subunit structure	Homodimer By similarity.
Induction	By nitrate.
Sequence similarities	Belongs to the nitrite and sulfite reductase 4Fe-4S domain family. Contains 1 Rieske domain.
Sequence caution	The sequence EAA31119.2 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Nitrate assimilation
Ligand	2Fe-2S 4Fe-4S FAD Flavoprotein Heme Iron Iron-sulfur Metal-binding NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	nitrate assimilation Inferred from electronic annotation. Source: UniProtKB-UniPathway
Molecular_function	2 iron, 2 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW 4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW NADP binding Inferred from electronic annotation. Source: InterPro flavin adenine dinucleotide binding Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW nitrite reductase [NAD(P)H] activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1176	1176	Nitrite reductase [NAD(P)H]		PRO_0000199960
Regions
	Domain	942 – 1094	153	Rieske; atypical
	Nucleotide binding	26 – 60	35	FAD Potential
	Nucleotide binding	183 – 215	33	NAD or NADP Potential
	Compositional bias	998 – 1054	57	Pro/Ser-rich
Sites
	Metal binding	717	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	723	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	757	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	761	1	Iron (siroheme axial ligand) By similarity
	Metal binding	761	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	981	1	Iron-sulfur (2Fe-2S) By similarity
	Metal binding	983	1	Iron-sulfur (2Fe-2S); via pros nitrogen By similarity
	Metal binding	1058	1	Iron-sulfur (2Fe-2S) By similarity
	Metal binding	1061	1	Iron-sulfur (2Fe-2S); via pros nitrogen By similarity
Experimental info
	Sequence conflict	357	1	T → S in L07391. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P38681 [UniParc]. Last modified December 4, 2007. Version 2. Checksum: 00CAA27C94D6A564	FASTA	1,176	127,382
	10 20 30 40 50 60 MANTSLDMAS STSPSPSPES TTTPRKRIVV VGLGMVGIAF IEKLIKLDTQ RQYEIVVIGE 70 80 90 100 110 120 EPHVAYNRVG LTSFFSHREV EQLYLNPLEW YKQHLQTSSL THHLSTAALS LSPATKSLTI 130 140 150 160 170 180 SPPPSTPSLT TLPYDHLILA TGSSALLPTS TPGHDASGVF VYRNIADLQS LITWSSDTQI 190 200 210 220 230 240 KGSTGVVVGG GLLGLEAAKA LMDLQVFGRV VVIERNGWVL SRQVDGEAGA LVLEGVRGLG 250 260 270 280 290 300 VEVLTRKRVK EVECDESKDE GEKEKKRVKG IRFEDGEYLA CSTICFAIGI KARDELAREA 310 320 330 340 350 360 GITCAERGGG GIVVDDSLQT SAPDVYAIGE CASWKGQTFG LIGPGVEMAD VLAFNFTQAH 370 380 390 400 410 420 LHTPRVFKRP DLSTKLKLLG VEVASFGDFF ADRDGPKELP PKLRRELKKS GGKAEVKALT 430 440 450 460 470 480 YKDPFLSVYK KYIFTSDGKY LLGGMMIGDT TDYVRLVPLV KTHKELDVPP SQLILGAKKS 490 500 510 520 530 540 GDDNGDDDLP DDTQICSCHN VTKADLVAPL KSGECTSLGD LKSCTKAGTG CGGCMPLVTS 550 560 570 580 590 600 IFNRTMASLG TEVKNNLCPH FPEYSRADLY NIISVKRLRT LPDVMREAGA DADSLGCEAC 610 620 630 640 650 660 KPAIASIFAS LWNDHVMSPA HHGLQDTNDR FMGNIQRNGT FSVVPRVAAG EITPEKLIVI 670 680 690 700 710 720 GEVAKEYNLY TKITGGQRID MFGAKKQDLL KIWKKLVDAG MESGHAYAKS LRTVKSCVGT 730 740 750 760 770 780 TWCRYGVGDS VGMAVRLEER YKGLRGPHKI KGGVSGCTRE CAEAGNKDFG LIATEKGFNI 790 800 810 820 830 840 LICGNGGTTP KHSVLLAKDV PPTNVIPIID RFLMFYIRTA DKLQRTARWL EALPGGIDYL 850 860 870 880 890 900 KEVILEDRLG ICASLEAQMQ ELVDSYFDEW AEALNNPAMQ ERFKQFANTD EGQPPMEVEI 910 920 930 940 950 960 DRGQERPVMW PREDEGGSAK ADFKGLRDKW SSTTWQPVLE ASYFQGADDL PNGISASIKR 970 980 990 1000 1010 1020 GDTQLAVWRI KGKYYASQQM CPHKRTFALS DGFVGTDPSP SSCSSSALPP SPPSTPPRSS 1030 1040 1050 1060 1070 1080 SPVTSPPQSP TSSATPATTA SSSCTTNPSG PASPWISCPF HKRNFSLTSG SCKNDNELSI 1090 1100 1110 1120 1130 1140 ATFDVEERDD GMVYIKLPPV DELDRELGTK KWMVKKGEAG EGQLRELDEL NKSKGVEGKK 1150 1160 1170 GRRGRKPGAS EAGKEVGKKL VEAVGGGGCG GPGLEW

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular cloning, characterization, and nucleotide sequence of nit-6, the structural gene for nitrite reductase in Neurospora crassa." Exley G.E., Colandene J.D., Garrett R.H. J. Bacteriol. 175:2379-2392(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987.
[2]	"The genome sequence of the filamentous fungus Neurospora crassa." Galagan J.E., Calvo S.E., Borkovich K.A., Selker E.U., Read N.D., Jaffe D.B., FitzHugh W., Ma L.-J., Smirnov S., Purcell S., Rehman B., Elkins T., Engels R., Wang S., Nielsen C.B., Butler J., Endrizzi M., Qui D. , Ianakiev P., Bell-Pedersen D., Nelson M.A., Werner-Washburne M., Selitrennikoff C.P., Kinsey J.A., Braun E.L., Zelter A., Schulte U., Kothe G.O., Jedd G., Mewes H.-W., Staben C., Marcotte E., Greenberg D., Roy A., Foley K., Naylor J., Stange-Thomann N., Barrett R., Gnerre S., Kamal M., Kamvysselis M., Mauceli E.W., Bielke C., Rudd S., Frishman D., Krystofova S., Rasmussen C., Metzenberg R.L., Perkins D.D., Kroken S., Cogoni C., Macino G., Catcheside D.E.A., Li W., Pratt R.J., Osmani S.A., DeSouza C.P.C., Glass N.L., Orbach M.J., Berglund J.A., Voelker R., Yarden O., Plamann M., Seiler S., Dunlap J.C., Radford A., Aramayo R., Natvig D.O., Alex L.A., Mannhaupt G., Ebbole D.J., Freitag M., Paulsen I., Sachs M.S., Lander E.S., Nusbaum C., Birren B.W. Nature 422:859-868(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L07391 Genomic DNA. No translation available. AABX02000016 Genomic DNA. Translation: EAA31119.2. Different initiation.
PIR	A49848.
RefSeq	XP_960355.2. XM_955262.2.
UniGene	Ncr.8742.
3D structure databases
ProteinModelPortal	P38681.
ModBase	Search...
Protein-protein interaction databases
STRING	5141.NCU04720.1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	EFNCRT00000004474; EFNCRP00000004474; EFNCRG00000004468.
GeneID	3876518.
KEGG	ncr:NCU04720.
Phylogenomic databases
eggNOG	COG1251.
HOGENOM	HOG000196164.
KO	K00362.
OrthoDB	EOG4M68RN.
Enzyme and pathway databases
BioCyc	MetaCyc:MONOMER-12533.
UniPathway	UPA00653.
Family and domain databases
Gene3D	2.102.10.10. 2 hits. 3.90.480.10. 1 hit.
InterPro	IPR007419. BFD-like_2Fe2S-bd_dom. IPR013027. FAD_pyr_nucl-diS_OxRdtase. IPR005117. NiRdtase/SiRdtase_haem-b_fer. IPR012744. Nitri_red_NirB. IPR006067. NO2/SO3_Rdtase_4Fe4S_dom. IPR006066. NO2/SO3_Rdtase_FeS/sirohaem_BS. IPR023753. Pyr_nucl-diS_OxRdtase_FAD/NAD. IPR001327. Pyr_OxRdtase_NAD-bd_dom. IPR017941. Rieske_2Fe-2S. [Graphical view]
Pfam	PF04324. Fer2_BFD. 1 hit. PF01077. NIR_SIR. 1 hit. PF03460. NIR_SIR_ferr. 1 hit. PF00070. Pyr_redox. 1 hit. PF07992. Pyr_redox_2. 1 hit. PF00355. Rieske. 1 hit. [Graphical view]
PRINTS	PR00368. FADPNR. PR00397. SIROHAEM.
SUPFAM	SSF55124. NiR_SiRalpha_1/3. 1 hit. SSF50022. Rieske_dom. 1 hit.
TIGRFAMs	TIGR02374. nitri_red_nirB. 1 hit.
PROSITE	PS00365. NIR_SIR. 1 hit. PS51296. RIESKE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NIR_NEUCR

Accession

Primary (citable) accession number: P38681
Secondary accession number(s): Q7RVB5

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	December 4, 2007
Last modified:	April 3, 2013
This is version 108 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program