Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Nitrite reductase [NAD(P)H]

Gene

nit-6

Organism
Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Catalytic activityi

Ammonia + 3 NAD(P)+ + 2 H2O = nitrite + 3 NAD(P)H.

Cofactori

Protein has several cofactor binding sites:
  • sirohemeNote: Binds 1 siroheme per subunit.
  • [4Fe-4S] clusterNote: Binds 1 [4Fe-4S] cluster per subunit.
  • FAD
  • [2Fe-2S] clusterPROSITE-ProRule annotationNote: Binds 1 [2Fe-2S] cluster per subunit.PROSITE-ProRule annotation

Pathwayi: nitrate reduction (assimilation)

This protein is involved in the pathway nitrate reduction (assimilation), which is part of Nitrogen metabolism.
View all proteins of this organism that are known to be involved in the pathway nitrate reduction (assimilation) and in Nitrogen metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi717 – 7171Iron-sulfur (4Fe-4S)By similarity
Metal bindingi723 – 7231Iron-sulfur (4Fe-4S)By similarity
Metal bindingi757 – 7571Iron-sulfur (4Fe-4S)By similarity
Metal bindingi761 – 7611Iron (siroheme axial ligand)By similarity
Metal bindingi761 – 7611Iron-sulfur (4Fe-4S)By similarity
Metal bindingi981 – 9811Iron-sulfur (2Fe-2S)PROSITE-ProRule annotation
Metal bindingi983 – 9831Iron-sulfur (2Fe-2S); via pros nitrogenPROSITE-ProRule annotation
Metal bindingi1058 – 10581Iron-sulfur (2Fe-2S)PROSITE-ProRule annotation
Metal bindingi1061 – 10611Iron-sulfur (2Fe-2S); via pros nitrogenPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi26 – 6035FADSequence analysisAdd
BLAST
Nucleotide bindingi183 – 21533NAD or NADPSequence analysisAdd
BLAST

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Nitrate assimilation

Keywords - Ligandi

2Fe-2S, 4Fe-4S, FAD, Flavoprotein, Heme, Iron, Iron-sulfur, Metal-binding, NADP

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-12533.
UniPathwayiUPA00653.

Names & Taxonomyi

Protein namesi
Recommended name:
Nitrite reductase [NAD(P)H] (EC:1.7.1.4)
Gene namesi
Name:nit-6
ORF Names:NCU04720
OrganismiNeurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987)
Taxonomic identifieri367110 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaSordariomycetesSordariomycetidaeSordarialesSordariaceaeNeurospora
Proteomesi
  • UP000001805 Componenti: Chromosome 5, Linkage Group VI

Organism-specific databases

EuPathDBiFungiDB:NCU04720.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 11761176Nitrite reductase [NAD(P)H]PRO_0000199960Add
BLAST

Expressioni

Inductioni

By nitrate.

Interactioni

Subunit structurei

Homodimer.By similarity

Structurei

3D structure databases

ProteinModelPortaliP38681.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini942 – 1094153Rieske; atypicalPROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi998 – 105457Pro/Ser-richAdd
BLAST

Sequence similaritiesi

Contains 1 Rieske domain.PROSITE-ProRule annotation

Phylogenomic databases

HOGENOMiHOG000196164.
InParanoidiP38681.
KOiK17877.
OrthoDBiEOG73RBM6.

Family and domain databases

Gene3Di2.102.10.10. 2 hits.
3.50.50.60. 2 hits.
3.90.480.10. 1 hit.
InterProiIPR007419. BFD-like_2Fe2S-bd_dom.
IPR023753. FAD/NAD-binding_dom.
IPR005117. NiRdtase/SiRdtase_haem-b_fer.
IPR012744. Nitri_red_NirB.
IPR006067. NO2/SO3_Rdtase_4Fe4S_dom.
IPR006066. NO2/SO3_Rdtase_FeS/sirohaem_BS.
IPR017941. Rieske_2Fe-2S.
[Graphical view]
PfamiPF04324. Fer2_BFD. 1 hit.
PF01077. NIR_SIR. 1 hit.
PF03460. NIR_SIR_ferr. 1 hit.
PF07992. Pyr_redox_2. 1 hit.
PF00355. Rieske. 1 hit.
[Graphical view]
PRINTSiPR00397. SIROHAEM.
SUPFAMiSSF50022. SSF50022. 2 hits.
SSF51905. SSF51905. 1 hit.
SSF55124. SSF55124. 1 hit.
TIGRFAMsiTIGR02374. nitri_red_nirB. 1 hit.
PROSITEiPS00365. NIR_SIR. 1 hit.
PS51296. RIESKE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P38681-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MANTSLDMAS STSPSPSPES TTTPRKRIVV VGLGMVGIAF IEKLIKLDTQ
60 70 80 90 100
RQYEIVVIGE EPHVAYNRVG LTSFFSHREV EQLYLNPLEW YKQHLQTSSL
110 120 130 140 150
THHLSTAALS LSPATKSLTI SPPPSTPSLT TLPYDHLILA TGSSALLPTS
160 170 180 190 200
TPGHDASGVF VYRNIADLQS LITWSSDTQI KGSTGVVVGG GLLGLEAAKA
210 220 230 240 250
LMDLQVFGRV VVIERNGWVL SRQVDGEAGA LVLEGVRGLG VEVLTRKRVK
260 270 280 290 300
EVECDESKDE GEKEKKRVKG IRFEDGEYLA CSTICFAIGI KARDELAREA
310 320 330 340 350
GITCAERGGG GIVVDDSLQT SAPDVYAIGE CASWKGQTFG LIGPGVEMAD
360 370 380 390 400
VLAFNFTQAH LHTPRVFKRP DLSTKLKLLG VEVASFGDFF ADRDGPKELP
410 420 430 440 450
PKLRRELKKS GGKAEVKALT YKDPFLSVYK KYIFTSDGKY LLGGMMIGDT
460 470 480 490 500
TDYVRLVPLV KTHKELDVPP SQLILGAKKS GDDNGDDDLP DDTQICSCHN
510 520 530 540 550
VTKADLVAPL KSGECTSLGD LKSCTKAGTG CGGCMPLVTS IFNRTMASLG
560 570 580 590 600
TEVKNNLCPH FPEYSRADLY NIISVKRLRT LPDVMREAGA DADSLGCEAC
610 620 630 640 650
KPAIASIFAS LWNDHVMSPA HHGLQDTNDR FMGNIQRNGT FSVVPRVAAG
660 670 680 690 700
EITPEKLIVI GEVAKEYNLY TKITGGQRID MFGAKKQDLL KIWKKLVDAG
710 720 730 740 750
MESGHAYAKS LRTVKSCVGT TWCRYGVGDS VGMAVRLEER YKGLRGPHKI
760 770 780 790 800
KGGVSGCTRE CAEAGNKDFG LIATEKGFNI LICGNGGTTP KHSVLLAKDV
810 820 830 840 850
PPTNVIPIID RFLMFYIRTA DKLQRTARWL EALPGGIDYL KEVILEDRLG
860 870 880 890 900
ICASLEAQMQ ELVDSYFDEW AEALNNPAMQ ERFKQFANTD EGQPPMEVEI
910 920 930 940 950
DRGQERPVMW PREDEGGSAK ADFKGLRDKW SSTTWQPVLE ASYFQGADDL
960 970 980 990 1000
PNGISASIKR GDTQLAVWRI KGKYYASQQM CPHKRTFALS DGFVGTDPSP
1010 1020 1030 1040 1050
SSCSSSALPP SPPSTPPRSS SPVTSPPQSP TSSATPATTA SSSCTTNPSG
1060 1070 1080 1090 1100
PASPWISCPF HKRNFSLTSG SCKNDNELSI ATFDVEERDD GMVYIKLPPV
1110 1120 1130 1140 1150
DELDRELGTK KWMVKKGEAG EGQLRELDEL NKSKGVEGKK GRRGRKPGAS
1160 1170
EAGKEVGKKL VEAVGGGGCG GPGLEW
Length:1,176
Mass (Da):127,382
Last modified:December 4, 2007 - v2
Checksum:i00CAA27C94D6A564
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti357 – 3571T → S in L07391 (PubMed:8096840).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L07391 Genomic DNA. No translation available.
CM002241 Genomic DNA. Translation: EAA31119.3.
PIRiA49848.
RefSeqiXP_960355.3. XM_955262.3.

Genome annotation databases

EnsemblFungiiEFNCRT00000004474; EFNCRP00000004474; EFNCRG00000004468.
GeneIDi3876518.
KEGGincr:NCU04720.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L07391 Genomic DNA. No translation available.
CM002241 Genomic DNA. Translation: EAA31119.3.
PIRiA49848.
RefSeqiXP_960355.3. XM_955262.3.

3D structure databases

ProteinModelPortaliP38681.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiEFNCRT00000004474; EFNCRP00000004474; EFNCRG00000004468.
GeneIDi3876518.
KEGGincr:NCU04720.

Organism-specific databases

EuPathDBiFungiDB:NCU04720.

Phylogenomic databases

HOGENOMiHOG000196164.
InParanoidiP38681.
KOiK17877.
OrthoDBiEOG73RBM6.

Enzyme and pathway databases

UniPathwayiUPA00653.
BioCyciMetaCyc:MONOMER-12533.

Family and domain databases

Gene3Di2.102.10.10. 2 hits.
3.50.50.60. 2 hits.
3.90.480.10. 1 hit.
InterProiIPR007419. BFD-like_2Fe2S-bd_dom.
IPR023753. FAD/NAD-binding_dom.
IPR005117. NiRdtase/SiRdtase_haem-b_fer.
IPR012744. Nitri_red_NirB.
IPR006067. NO2/SO3_Rdtase_4Fe4S_dom.
IPR006066. NO2/SO3_Rdtase_FeS/sirohaem_BS.
IPR017941. Rieske_2Fe-2S.
[Graphical view]
PfamiPF04324. Fer2_BFD. 1 hit.
PF01077. NIR_SIR. 1 hit.
PF03460. NIR_SIR_ferr. 1 hit.
PF07992. Pyr_redox_2. 1 hit.
PF00355. Rieske. 1 hit.
[Graphical view]
PRINTSiPR00397. SIROHAEM.
SUPFAMiSSF50022. SSF50022. 2 hits.
SSF51905. SSF51905. 1 hit.
SSF55124. SSF55124. 1 hit.
TIGRFAMsiTIGR02374. nitri_red_nirB. 1 hit.
PROSITEiPS00365. NIR_SIR. 1 hit.
PS51296. RIESKE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular cloning, characterization, and nucleotide sequence of nit-6, the structural gene for nitrite reductase in Neurospora crassa."
    Exley G.E., Colandene J.D., Garrett R.H.
    J. Bacteriol. 175:2379-2392(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987.
  2. "The genome sequence of the filamentous fungus Neurospora crassa."
    Galagan J.E., Calvo S.E., Borkovich K.A., Selker E.U., Read N.D., Jaffe D.B., FitzHugh W., Ma L.-J., Smirnov S., Purcell S., Rehman B., Elkins T., Engels R., Wang S., Nielsen C.B., Butler J., Endrizzi M., Qui D.
    , Ianakiev P., Bell-Pedersen D., Nelson M.A., Werner-Washburne M., Selitrennikoff C.P., Kinsey J.A., Braun E.L., Zelter A., Schulte U., Kothe G.O., Jedd G., Mewes H.-W., Staben C., Marcotte E., Greenberg D., Roy A., Foley K., Naylor J., Stange-Thomann N., Barrett R., Gnerre S., Kamal M., Kamvysselis M., Mauceli E.W., Bielke C., Rudd S., Frishman D., Krystofova S., Rasmussen C., Metzenberg R.L., Perkins D.D., Kroken S., Cogoni C., Macino G., Catcheside D.E.A., Li W., Pratt R.J., Osmani S.A., DeSouza C.P.C., Glass N.L., Orbach M.J., Berglund J.A., Voelker R., Yarden O., Plamann M., Seiler S., Dunlap J.C., Radford A., Aramayo R., Natvig D.O., Alex L.A., Mannhaupt G., Ebbole D.J., Freitag M., Paulsen I., Sachs M.S., Lander E.S., Nusbaum C., Birren B.W.
    Nature 422:859-868(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987.

Entry informationi

Entry nameiNIR_NEUCR
AccessioniPrimary (citable) accession number: P38681
Secondary accession number(s): Q7RVB5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: December 4, 2007
Last modified: June 8, 2016
This is version 126 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.