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Protein

Nitrite reductase [NAD(P)H]

Gene

nit-6

Organism
Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Catalytic activityi

Ammonia + 3 NAD(P)+ + 2 H2O = nitrite + 3 NAD(P)H.

Cofactori

Protein has several cofactor binding sites:
  • sirohemeNote: Binds 1 siroheme per subunit.
  • [4Fe-4S] clusterNote: Binds 1 [4Fe-4S] cluster per subunit.
  • FAD
  • [2Fe-2S] clusterPROSITE-ProRule annotationNote: Binds 1 [2Fe-2S] cluster per subunit.PROSITE-ProRule annotation

Pathwayi: nitrate reduction (assimilation)

This protein is involved in the pathway nitrate reduction (assimilation), which is part of Nitrogen metabolism.
View all proteins of this organism that are known to be involved in the pathway nitrate reduction (assimilation) and in Nitrogen metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi717Iron-sulfur (4Fe-4S)By similarity1
Metal bindingi723Iron-sulfur (4Fe-4S)By similarity1
Metal bindingi757Iron-sulfur (4Fe-4S)By similarity1
Metal bindingi761Iron (siroheme axial ligand)By similarity1
Metal bindingi761Iron-sulfur (4Fe-4S)By similarity1
Metal bindingi981Iron-sulfur (2Fe-2S)PROSITE-ProRule annotation1
Metal bindingi983Iron-sulfur (2Fe-2S); via pros nitrogenPROSITE-ProRule annotation1
Metal bindingi1058Iron-sulfur (2Fe-2S)PROSITE-ProRule annotation1
Metal bindingi1061Iron-sulfur (2Fe-2S); via pros nitrogenPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi26 – 60FADSequence analysisAdd BLAST35
Nucleotide bindingi183 – 215NAD or NADPSequence analysisAdd BLAST33

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
Biological processNitrate assimilation
Ligand2Fe-2S, 4Fe-4S, FAD, Flavoprotein, Heme, Iron, Iron-sulfur, Metal-binding, NADP

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-12533
UniPathwayiUPA00653

Names & Taxonomyi

Protein namesi
Recommended name:
Nitrite reductase [NAD(P)H] (EC:1.7.1.4)
Gene namesi
Name:nit-6
ORF Names:NCU04720
OrganismiNeurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987)
Taxonomic identifieri367110 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaSordariomycetesSordariomycetidaeSordarialesSordariaceaeNeurospora
Proteomesi
  • UP000001805 Componenti: Chromosome 5, Linkage Group VI

Organism-specific databases

EuPathDBiFungiDB:NCU04720

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001999601 – 1176Nitrite reductase [NAD(P)H]Add BLAST1176

Proteomic databases

PRIDEiP38681

Expressioni

Inductioni

By nitrate.

Interactioni

Subunit structurei

Homodimer.By similarity

Structurei

3D structure databases

ProteinModelPortaliP38681
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini942 – 1094Rieske; atypicalPROSITE-ProRule annotationAdd BLAST153

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi998 – 1054Pro/Ser-richAdd BLAST57

Sequence similaritiesi

Phylogenomic databases

HOGENOMiHOG000196164
InParanoidiP38681
KOiK17877
OrthoDBiEOG092C0BFS

Family and domain databases

CDDicd03529 Rieske_NirD, 1 hit
Gene3Di3.50.50.60, 3 hits
InterProiView protein in InterPro
IPR007419 BFD-like_2Fe2S-bd_dom
IPR036188 FAD/NAD-bd_sf
IPR023753 FAD/NAD-binding_dom
IPR005117 NiRdtase/SiRdtase_haem-b_fer
IPR036136 Nit/Sulf_reduc_fer_like_dom_sf
IPR012744 Nitri_red_NirB
IPR017881 Nitrite_Rdtase_lsu_NirD_bac
IPR006067 NO2/SO3_Rdtase_4Fe4S_dom
IPR006066 NO2/SO3_Rdtase_FeS/sirohaem_BS
IPR017941 Rieske_2Fe-2S
IPR036922 Rieske_2Fe-2S_sf
PfamiView protein in Pfam
PF04324 Fer2_BFD, 1 hit
PF01077 NIR_SIR, 1 hit
PF03460 NIR_SIR_ferr, 1 hit
PF07992 Pyr_redox_2, 1 hit
PF00355 Rieske, 1 hit
PRINTSiPR00397 SIROHAEM
SUPFAMiSSF50022 SSF50022, 2 hits
SSF51905 SSF51905, 1 hit
SSF55124 SSF55124, 1 hit
TIGRFAMsiTIGR02374 nitri_red_nirB, 1 hit
PROSITEiView protein in PROSITE
PS00365 NIR_SIR, 1 hit
PS51296 RIESKE, 1 hit

Sequencei

Sequence statusi: Complete.

P38681-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MANTSLDMAS STSPSPSPES TTTPRKRIVV VGLGMVGIAF IEKLIKLDTQ
60 70 80 90 100
RQYEIVVIGE EPHVAYNRVG LTSFFSHREV EQLYLNPLEW YKQHLQTSSL
110 120 130 140 150
THHLSTAALS LSPATKSLTI SPPPSTPSLT TLPYDHLILA TGSSALLPTS
160 170 180 190 200
TPGHDASGVF VYRNIADLQS LITWSSDTQI KGSTGVVVGG GLLGLEAAKA
210 220 230 240 250
LMDLQVFGRV VVIERNGWVL SRQVDGEAGA LVLEGVRGLG VEVLTRKRVK
260 270 280 290 300
EVECDESKDE GEKEKKRVKG IRFEDGEYLA CSTICFAIGI KARDELAREA
310 320 330 340 350
GITCAERGGG GIVVDDSLQT SAPDVYAIGE CASWKGQTFG LIGPGVEMAD
360 370 380 390 400
VLAFNFTQAH LHTPRVFKRP DLSTKLKLLG VEVASFGDFF ADRDGPKELP
410 420 430 440 450
PKLRRELKKS GGKAEVKALT YKDPFLSVYK KYIFTSDGKY LLGGMMIGDT
460 470 480 490 500
TDYVRLVPLV KTHKELDVPP SQLILGAKKS GDDNGDDDLP DDTQICSCHN
510 520 530 540 550
VTKADLVAPL KSGECTSLGD LKSCTKAGTG CGGCMPLVTS IFNRTMASLG
560 570 580 590 600
TEVKNNLCPH FPEYSRADLY NIISVKRLRT LPDVMREAGA DADSLGCEAC
610 620 630 640 650
KPAIASIFAS LWNDHVMSPA HHGLQDTNDR FMGNIQRNGT FSVVPRVAAG
660 670 680 690 700
EITPEKLIVI GEVAKEYNLY TKITGGQRID MFGAKKQDLL KIWKKLVDAG
710 720 730 740 750
MESGHAYAKS LRTVKSCVGT TWCRYGVGDS VGMAVRLEER YKGLRGPHKI
760 770 780 790 800
KGGVSGCTRE CAEAGNKDFG LIATEKGFNI LICGNGGTTP KHSVLLAKDV
810 820 830 840 850
PPTNVIPIID RFLMFYIRTA DKLQRTARWL EALPGGIDYL KEVILEDRLG
860 870 880 890 900
ICASLEAQMQ ELVDSYFDEW AEALNNPAMQ ERFKQFANTD EGQPPMEVEI
910 920 930 940 950
DRGQERPVMW PREDEGGSAK ADFKGLRDKW SSTTWQPVLE ASYFQGADDL
960 970 980 990 1000
PNGISASIKR GDTQLAVWRI KGKYYASQQM CPHKRTFALS DGFVGTDPSP
1010 1020 1030 1040 1050
SSCSSSALPP SPPSTPPRSS SPVTSPPQSP TSSATPATTA SSSCTTNPSG
1060 1070 1080 1090 1100
PASPWISCPF HKRNFSLTSG SCKNDNELSI ATFDVEERDD GMVYIKLPPV
1110 1120 1130 1140 1150
DELDRELGTK KWMVKKGEAG EGQLRELDEL NKSKGVEGKK GRRGRKPGAS
1160 1170
EAGKEVGKKL VEAVGGGGCG GPGLEW
Length:1,176
Mass (Da):127,382
Last modified:December 4, 2007 - v2
Checksum:i00CAA27C94D6A564
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti357T → S in L07391 (PubMed:8096840).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L07391 Genomic DNA No translation available.
CM002241 Genomic DNA Translation: EAA31119.3
PIRiA49848
RefSeqiXP_960355.3, XM_955262.3

Genome annotation databases

EnsemblFungiiEAA31119; EAA31119; NCU04720
GeneIDi3876518
KEGGincr:NCU04720

Similar proteinsi

Entry informationi

Entry nameiNIR_NEUCR
AccessioniPrimary (citable) accession number: P38681
Secondary accession number(s): Q7RVB5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: December 4, 2007
Last modified: May 23, 2018
This is version 137 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

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