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Protein

Carboxy-cis,cis-muconate cyclase

Gene

NCU04071

Organism
Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes a syn cycloisomerization. Also possesses mle activity.

Catalytic activityi

3-carboxy-2,5-dihydro-5-oxofuran-2-acetate = 3-carboxy-cis,cis-muconate.

Pathwayi: beta-ketoadipate pathway

This protein is involved in step 1 of the subpathway that synthesizes 3-carboxy-cis,cis-muconate from 3-carboxy-2,5-dihydro-5-oxofuran-2-acetate.
Proteins known to be involved in this subpathway in this organism are:
  1. Carboxy-cis,cis-muconate cyclase (NCU04071)
This subpathway is part of the pathway beta-ketoadipate pathway, which is itself part of Aromatic compound metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 3-carboxy-cis,cis-muconate from 3-carboxy-2,5-dihydro-5-oxofuran-2-acetate, the pathway beta-ketoadipate pathway and in Aromatic compound metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei1491
Active sitei1971
Active sitei2131
Active sitei2751

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Isomerase

Enzyme and pathway databases

UniPathwayiUPA00157; UER00308.

Names & Taxonomyi

Protein namesi
Recommended name:
Carboxy-cis,cis-muconate cyclase (EC:5.5.1.5)
Alternative name(s):
3-carboxy-cis,cis-muconate lactonizing enzyme
Short name:
CMLE
Gene namesi
ORF Names:NCU04071
OrganismiNeurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987)
Taxonomic identifieri367110 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaSordariomycetesSordariomycetidaeSordarialesSordariaceaeNeurospora
Proteomesi
  • UP000001805 Componenti: Chromosome 5, Linkage Group VI

Organism-specific databases

EuPathDBiFungiDB:NCU04071.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00001711272 – 366Carboxy-cis,cis-muconate cyclaseAdd BLAST365

Interactioni

Subunit structurei

Homotetramer.

Structurei

Secondary structure

1366
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi3 – 14Combined sources12
Beta strandi16 – 23Combined sources8
Turni24 – 27Combined sources4
Beta strandi28 – 36Combined sources9
Beta strandi44 – 48Combined sources5
Beta strandi52 – 60Combined sources9
Beta strandi62 – 70Combined sources9
Beta strandi73 – 81Combined sources9
Helixi86 – 89Combined sources4
Beta strandi96 – 102Combined sources7
Beta strandi110 – 118Combined sources9
Beta strandi121 – 127Combined sources7
Beta strandi133 – 141Combined sources9
Beta strandi148 – 153Combined sources6
Beta strandi157 – 164Combined sources8
Turni165 – 168Combined sources4
Beta strandi169 – 175Combined sources7
Beta strandi181 – 188Combined sources8
Beta strandi196 – 201Combined sources6
Beta strandi205 – 212Combined sources8
Turni213 – 216Combined sources4
Beta strandi217 – 223Combined sources7
Turni225 – 227Combined sources3
Beta strandi230 – 240Combined sources11
Turni249 – 251Combined sources3
Beta strandi252 – 263Combined sources12
Beta strandi267 – 278Combined sources12
Beta strandi284 – 290Combined sources7
Beta strandi296 – 304Combined sources9
Beta strandi315 – 317Combined sources3
Beta strandi324 – 328Combined sources5
Beta strandi330 – 332Combined sources3
Beta strandi334 – 341Combined sources8
Beta strandi344 – 352Combined sources9
Beta strandi358 – 365Combined sources8

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1JOFX-ray2.50A/B/C/D/E/F/G/H2-366[»]
ProteinModelPortaliP38677.
SMRiP38677.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP38677.

Family & Domainsi

Sequence similaritiesi

Belongs to the cycloisomerase 2 family.Curated

Phylogenomic databases

HOGENOMiHOG000216586.
InParanoidiP38677.
KOiK14334.
OMAiTPPGAIF.
OrthoDBiEOG092C335T.

Family and domain databases

Gene3Di2.130.10.10. 1 hit.
InterProiIPR019405. Lactonase_7-beta_prop.
IPR015943. WD40/YVTN_repeat-like_dom.
[Graphical view]
PfamiPF10282. Lactonase. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P38677-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPLHHLMIGT WTPPGAIFTV QFDDEKLTCK LIKRTEIPQD EPISWMTFDH
60 70 80 90 100
ERKNIYGAAM KKWSSFAVKS PTEIVHEASH PIGGHPRAND ADTNTRAIFL
110 120 130 140 150
LAAKQPPYAV YANPFYKFAG YGNVFSVSET GKLEKNVQNY EYQENTGIHG
160 170 180 190 200
MVFDPTETYL YSADLTANKL WTHRKLASGE VELVGSVDAP DPGDHPRWVA
210 220 230 240 250
MHPTGNYLYA LMEAGNRICE YVIDPATHMP VYTHHSFPLI PPGIPDRDPE
260 270 280 290 300
TGKGLYRADV CALTFSGKYM FASSRANKFE LQGYIAGFKL RDCGSIEKQL
310 320 330 340 350
FLSPTPTSGG HSNAVSPCPW SDEWMAITDD QEGWLEIYRW KDEFLHRVAR
360
VRIPEPGFGM NAIWYD
Length:366
Mass (Da):41,329
Last modified:January 23, 2007 - v3
Checksum:iE71BA50509E72873
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L27538 mRNA. Translation: AAA21020.1.
CM002241 Genomic DNA. Translation: EAA28450.1.
PIRiA55525.
RefSeqiXP_957686.1. XM_952593.2.

Genome annotation databases

EnsemblFungiiEAA28450; EAA28450; NCU04071.
GeneIDi3873776.
KEGGincr:NCU04071.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L27538 mRNA. Translation: AAA21020.1.
CM002241 Genomic DNA. Translation: EAA28450.1.
PIRiA55525.
RefSeqiXP_957686.1. XM_952593.2.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1JOFX-ray2.50A/B/C/D/E/F/G/H2-366[»]
ProteinModelPortaliP38677.
SMRiP38677.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiEAA28450; EAA28450; NCU04071.
GeneIDi3873776.
KEGGincr:NCU04071.

Organism-specific databases

EuPathDBiFungiDB:NCU04071.

Phylogenomic databases

HOGENOMiHOG000216586.
InParanoidiP38677.
KOiK14334.
OMAiTPPGAIF.
OrthoDBiEOG092C335T.

Enzyme and pathway databases

UniPathwayiUPA00157; UER00308.

Miscellaneous databases

EvolutionaryTraceiP38677.

Family and domain databases

Gene3Di2.130.10.10. 1 hit.
InterProiIPR019405. Lactonase_7-beta_prop.
IPR015943. WD40/YVTN_repeat-like_dom.
[Graphical view]
PfamiPF10282. Lactonase. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCMLE_NEUCR
AccessioniPrimary (citable) accession number: P38677
Secondary accession number(s): Q7RV80
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: January 23, 2007
Last modified: November 2, 2016
This is version 106 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.