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P38677

- CMLE_NEUCR

UniProt

P38677 - CMLE_NEUCR

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Protein

Carboxy-cis,cis-muconate cyclase

Gene

NCU04071

Organism
Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Catalyzes a syn cycloisomerization. Also possesses mle activity.

Catalytic activityi

3-carboxy-2,5-dihydro-5-oxofuran-2-acetate = 3-carboxy-cis,cis-muconate.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei149 – 1491
Active sitei197 – 1971
Active sitei213 – 2131
Active sitei275 – 2751

GO - Molecular functioni

  1. carboxy-cis,cis-muconate cyclase activity Source: UniProtKB-EC

GO - Biological processi

  1. beta-ketoadipate pathway Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Isomerase

Enzyme and pathway databases

UniPathwayiUPA00157; UER00308.

Names & Taxonomyi

Protein namesi
Recommended name:
Carboxy-cis,cis-muconate cyclase (EC:5.5.1.5)
Alternative name(s):
3-carboxy-cis,cis-muconate lactonizing enzyme
Short name:
CMLE
Gene namesi
ORF Names:NCU04071
OrganismiNeurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987)
Taxonomic identifieri367110 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaSordariomycetesSordariomycetidaeSordarialesSordariaceaeNeurospora
ProteomesiUP000001805: Chromosome 5, Linkage Group VI

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed1 Publication
Chaini2 – 366365Carboxy-cis,cis-muconate cyclasePRO_0000171127Add
BLAST

Interactioni

Subunit structurei

Homotetramer.

Protein-protein interaction databases

STRINGi5141.NCU04071.1.

Structurei

Secondary structure

1
366
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi3 – 1412
Beta strandi16 – 238
Turni24 – 274
Beta strandi28 – 369
Beta strandi44 – 485
Beta strandi52 – 609
Beta strandi62 – 709
Beta strandi73 – 819
Helixi86 – 894
Beta strandi96 – 1027
Beta strandi110 – 1189
Beta strandi121 – 1277
Beta strandi133 – 1419
Beta strandi148 – 1536
Beta strandi157 – 1648
Turni165 – 1684
Beta strandi169 – 1757
Beta strandi181 – 1888
Beta strandi196 – 2016
Beta strandi205 – 2128
Turni213 – 2164
Beta strandi217 – 2237
Turni225 – 2273
Beta strandi230 – 24011
Turni249 – 2513
Beta strandi252 – 26312
Beta strandi267 – 27812
Beta strandi284 – 2907
Beta strandi296 – 3049
Beta strandi315 – 3173
Beta strandi324 – 3285
Beta strandi330 – 3323
Beta strandi334 – 3418
Beta strandi344 – 3529
Beta strandi358 – 3658

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1JOFX-ray2.50A/B/C/D/E/F/G/H2-366[»]
ProteinModelPortaliP38677.
SMRiP38677. Positions 2-366.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP38677.

Family & Domainsi

Sequence similaritiesi

Belongs to the cycloisomerase 2 family.Curated

Phylogenomic databases

eggNOGiNOG133463.
HOGENOMiHOG000216586.
InParanoidiP38677.
KOiK14334.
OMAiTPPGAIF.
OrthoDBiEOG7VB2QX.

Family and domain databases

Gene3Di2.130.10.10. 1 hit.
InterProiIPR019405. Lactonase_7-beta_prop.
IPR015943. WD40/YVTN_repeat-like_dom.
[Graphical view]
PfamiPF10282. Lactonase. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P38677-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MPLHHLMIGT WTPPGAIFTV QFDDEKLTCK LIKRTEIPQD EPISWMTFDH
60 70 80 90 100
ERKNIYGAAM KKWSSFAVKS PTEIVHEASH PIGGHPRAND ADTNTRAIFL
110 120 130 140 150
LAAKQPPYAV YANPFYKFAG YGNVFSVSET GKLEKNVQNY EYQENTGIHG
160 170 180 190 200
MVFDPTETYL YSADLTANKL WTHRKLASGE VELVGSVDAP DPGDHPRWVA
210 220 230 240 250
MHPTGNYLYA LMEAGNRICE YVIDPATHMP VYTHHSFPLI PPGIPDRDPE
260 270 280 290 300
TGKGLYRADV CALTFSGKYM FASSRANKFE LQGYIAGFKL RDCGSIEKQL
310 320 330 340 350
FLSPTPTSGG HSNAVSPCPW SDEWMAITDD QEGWLEIYRW KDEFLHRVAR
360
VRIPEPGFGM NAIWYD
Length:366
Mass (Da):41,329
Last modified:January 23, 2007 - v3
Checksum:iE71BA50509E72873
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L27538 mRNA. Translation: AAA21020.1.
CM002241 Genomic DNA. Translation: EAA28450.1.
PIRiA55525.
RefSeqiXP_957686.1. XM_952593.1.

Genome annotation databases

EnsemblFungiiEFNCRT00000003530; EFNCRP00000003530; EFNCRG00000003526.
GeneIDi3873776.
KEGGincr:NCU04071.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L27538 mRNA. Translation: AAA21020.1 .
CM002241 Genomic DNA. Translation: EAA28450.1 .
PIRi A55525.
RefSeqi XP_957686.1. XM_952593.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1JOF X-ray 2.50 A/B/C/D/E/F/G/H 2-366 [» ]
ProteinModelPortali P38677.
SMRi P38677. Positions 2-366.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 5141.NCU04071.1.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii EFNCRT00000003530 ; EFNCRP00000003530 ; EFNCRG00000003526 .
GeneIDi 3873776.
KEGGi ncr:NCU04071.

Phylogenomic databases

eggNOGi NOG133463.
HOGENOMi HOG000216586.
InParanoidi P38677.
KOi K14334.
OMAi TPPGAIF.
OrthoDBi EOG7VB2QX.

Enzyme and pathway databases

UniPathwayi UPA00157 ; UER00308 .

Miscellaneous databases

EvolutionaryTracei P38677.

Family and domain databases

Gene3Di 2.130.10.10. 1 hit.
InterProi IPR019405. Lactonase_7-beta_prop.
IPR015943. WD40/YVTN_repeat-like_dom.
[Graphical view ]
Pfami PF10282. Lactonase. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "3-carboxy-cis,cis-muconate lactonizing enzyme from Neurospora crassa: an alternate cycloisomerase motif."
    Mazur P., Henzel W.J., Mattoo S., Kozarich J.W.
    J. Bacteriol. 176:1718-1728(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 2-41, CHARACTERIZATION.
    Strain: ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987.
  2. "The genome sequence of the filamentous fungus Neurospora crassa."
    Galagan J.E., Calvo S.E., Borkovich K.A., Selker E.U., Read N.D., Jaffe D.B., FitzHugh W., Ma L.-J., Smirnov S., Purcell S., Rehman B., Elkins T., Engels R., Wang S., Nielsen C.B., Butler J., Endrizzi M., Qui D.
    , Ianakiev P., Bell-Pedersen D., Nelson M.A., Werner-Washburne M., Selitrennikoff C.P., Kinsey J.A., Braun E.L., Zelter A., Schulte U., Kothe G.O., Jedd G., Mewes H.-W., Staben C., Marcotte E., Greenberg D., Roy A., Foley K., Naylor J., Stange-Thomann N., Barrett R., Gnerre S., Kamal M., Kamvysselis M., Mauceli E.W., Bielke C., Rudd S., Frishman D., Krystofova S., Rasmussen C., Metzenberg R.L., Perkins D.D., Kroken S., Cogoni C., Macino G., Catcheside D.E.A., Li W., Pratt R.J., Osmani S.A., DeSouza C.P.C., Glass N.L., Orbach M.J., Berglund J.A., Voelker R., Yarden O., Plamann M., Seiler S., Dunlap J.C., Radford A., Aramayo R., Natvig D.O., Alex L.A., Mannhaupt G., Ebbole D.J., Freitag M., Paulsen I., Sachs M.S., Lander E.S., Nusbaum C., Birren B.W.
    Nature 422:859-868(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987.
  3. "The structure of Neurospora crassa 3-carboxy-cis,cis-muconate lactonizing enzyme, a beta propeller cycloisomerase."
    Kajander T., Merckel M.C., Thompson A., Deacon A.M., Mazur P., Kozarich J.W., Goldman A.
    Structure 10:483-492(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS).

Entry informationi

Entry nameiCMLE_NEUCR
AccessioniPrimary (citable) accession number: P38677
Secondary accession number(s): Q7RV80
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: January 23, 2007
Last modified: October 29, 2014
This is version 95 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3