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Protein

Carboxy-cis,cis-muconate cyclase

Gene

NCU04071

Organism
Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes a syn cycloisomerization. Also possesses mle activity.

Catalytic activityi

3-carboxy-2,5-dihydro-5-oxofuran-2-acetate = 3-carboxy-cis,cis-muconate.

Pathway: beta-ketoadipate pathway

This protein is involved in step 1 of the subpathway that synthesizes 3-carboxy-cis,cis-muconate from 3-carboxy-2,5-dihydro-5-oxofuran-2-acetate.
Proteins known to be involved in this subpathway in this organism are:
  1. Carboxy-cis,cis-muconate cyclase (NCU04071)
This subpathway is part of the pathway beta-ketoadipate pathway, which is itself part of Aromatic compound metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 3-carboxy-cis,cis-muconate from 3-carboxy-2,5-dihydro-5-oxofuran-2-acetate, the pathway beta-ketoadipate pathway and in Aromatic compound metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei149 – 1491
Active sitei197 – 1971
Active sitei213 – 2131
Active sitei275 – 2751

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Isomerase

Enzyme and pathway databases

UniPathwayiUPA00157; UER00308.

Names & Taxonomyi

Protein namesi
Recommended name:
Carboxy-cis,cis-muconate cyclase (EC:5.5.1.5)
Alternative name(s):
3-carboxy-cis,cis-muconate lactonizing enzyme
Short name:
CMLE
Gene namesi
ORF Names:NCU04071
OrganismiNeurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987)
Taxonomic identifieri367110 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaSordariomycetesSordariomycetidaeSordarialesSordariaceaeNeurospora
ProteomesiUP000001805 Componenti: Chromosome 5, Linkage Group VI

Organism-specific databases

EuPathDBiFungiDB:NCU04071.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed1 Publication
Chaini2 – 366365Carboxy-cis,cis-muconate cyclasePRO_0000171127Add
BLAST

Interactioni

Subunit structurei

Homotetramer.

Structurei

Secondary structure

1
366
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi3 – 1412Combined sources
Beta strandi16 – 238Combined sources
Turni24 – 274Combined sources
Beta strandi28 – 369Combined sources
Beta strandi44 – 485Combined sources
Beta strandi52 – 609Combined sources
Beta strandi62 – 709Combined sources
Beta strandi73 – 819Combined sources
Helixi86 – 894Combined sources
Beta strandi96 – 1027Combined sources
Beta strandi110 – 1189Combined sources
Beta strandi121 – 1277Combined sources
Beta strandi133 – 1419Combined sources
Beta strandi148 – 1536Combined sources
Beta strandi157 – 1648Combined sources
Turni165 – 1684Combined sources
Beta strandi169 – 1757Combined sources
Beta strandi181 – 1888Combined sources
Beta strandi196 – 2016Combined sources
Beta strandi205 – 2128Combined sources
Turni213 – 2164Combined sources
Beta strandi217 – 2237Combined sources
Turni225 – 2273Combined sources
Beta strandi230 – 24011Combined sources
Turni249 – 2513Combined sources
Beta strandi252 – 26312Combined sources
Beta strandi267 – 27812Combined sources
Beta strandi284 – 2907Combined sources
Beta strandi296 – 3049Combined sources
Beta strandi315 – 3173Combined sources
Beta strandi324 – 3285Combined sources
Beta strandi330 – 3323Combined sources
Beta strandi334 – 3418Combined sources
Beta strandi344 – 3529Combined sources
Beta strandi358 – 3658Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1JOFX-ray2.50A/B/C/D/E/F/G/H2-366[»]
ProteinModelPortaliP38677.
SMRiP38677. Positions 2-366.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP38677.

Family & Domainsi

Sequence similaritiesi

Belongs to the cycloisomerase 2 family.Curated

Phylogenomic databases

eggNOGiNOG133463.
HOGENOMiHOG000216586.
InParanoidiP38677.
KOiK14334.
OMAiTPPGAIF.
OrthoDBiEOG7VB2QX.

Family and domain databases

Gene3Di2.130.10.10. 1 hit.
InterProiIPR019405. Lactonase_7-beta_prop.
IPR015943. WD40/YVTN_repeat-like_dom.
[Graphical view]
PfamiPF10282. Lactonase. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P38677-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPLHHLMIGT WTPPGAIFTV QFDDEKLTCK LIKRTEIPQD EPISWMTFDH
60 70 80 90 100
ERKNIYGAAM KKWSSFAVKS PTEIVHEASH PIGGHPRAND ADTNTRAIFL
110 120 130 140 150
LAAKQPPYAV YANPFYKFAG YGNVFSVSET GKLEKNVQNY EYQENTGIHG
160 170 180 190 200
MVFDPTETYL YSADLTANKL WTHRKLASGE VELVGSVDAP DPGDHPRWVA
210 220 230 240 250
MHPTGNYLYA LMEAGNRICE YVIDPATHMP VYTHHSFPLI PPGIPDRDPE
260 270 280 290 300
TGKGLYRADV CALTFSGKYM FASSRANKFE LQGYIAGFKL RDCGSIEKQL
310 320 330 340 350
FLSPTPTSGG HSNAVSPCPW SDEWMAITDD QEGWLEIYRW KDEFLHRVAR
360
VRIPEPGFGM NAIWYD
Length:366
Mass (Da):41,329
Last modified:January 23, 2007 - v3
Checksum:iE71BA50509E72873
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L27538 mRNA. Translation: AAA21020.1.
CM002241 Genomic DNA. Translation: EAA28450.1.
PIRiA55525.
RefSeqiXP_957686.1. XM_952593.2.

Genome annotation databases

EnsemblFungiiEFNCRT00000003530; EFNCRP00000003530; EFNCRG00000003526.
GeneIDi3873776.
KEGGincr:NCU04071.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L27538 mRNA. Translation: AAA21020.1.
CM002241 Genomic DNA. Translation: EAA28450.1.
PIRiA55525.
RefSeqiXP_957686.1. XM_952593.2.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1JOFX-ray2.50A/B/C/D/E/F/G/H2-366[»]
ProteinModelPortaliP38677.
SMRiP38677. Positions 2-366.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiEFNCRT00000003530; EFNCRP00000003530; EFNCRG00000003526.
GeneIDi3873776.
KEGGincr:NCU04071.

Organism-specific databases

EuPathDBiFungiDB:NCU04071.

Phylogenomic databases

eggNOGiNOG133463.
HOGENOMiHOG000216586.
InParanoidiP38677.
KOiK14334.
OMAiTPPGAIF.
OrthoDBiEOG7VB2QX.

Enzyme and pathway databases

UniPathwayiUPA00157; UER00308.

Miscellaneous databases

EvolutionaryTraceiP38677.

Family and domain databases

Gene3Di2.130.10.10. 1 hit.
InterProiIPR019405. Lactonase_7-beta_prop.
IPR015943. WD40/YVTN_repeat-like_dom.
[Graphical view]
PfamiPF10282. Lactonase. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "3-carboxy-cis,cis-muconate lactonizing enzyme from Neurospora crassa: an alternate cycloisomerase motif."
    Mazur P., Henzel W.J., Mattoo S., Kozarich J.W.
    J. Bacteriol. 176:1718-1728(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 2-41, CHARACTERIZATION.
    Strain: ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987.
  2. "The genome sequence of the filamentous fungus Neurospora crassa."
    Galagan J.E., Calvo S.E., Borkovich K.A., Selker E.U., Read N.D., Jaffe D.B., FitzHugh W., Ma L.-J., Smirnov S., Purcell S., Rehman B., Elkins T., Engels R., Wang S., Nielsen C.B., Butler J., Endrizzi M., Qui D.
    , Ianakiev P., Bell-Pedersen D., Nelson M.A., Werner-Washburne M., Selitrennikoff C.P., Kinsey J.A., Braun E.L., Zelter A., Schulte U., Kothe G.O., Jedd G., Mewes H.-W., Staben C., Marcotte E., Greenberg D., Roy A., Foley K., Naylor J., Stange-Thomann N., Barrett R., Gnerre S., Kamal M., Kamvysselis M., Mauceli E.W., Bielke C., Rudd S., Frishman D., Krystofova S., Rasmussen C., Metzenberg R.L., Perkins D.D., Kroken S., Cogoni C., Macino G., Catcheside D.E.A., Li W., Pratt R.J., Osmani S.A., DeSouza C.P.C., Glass N.L., Orbach M.J., Berglund J.A., Voelker R., Yarden O., Plamann M., Seiler S., Dunlap J.C., Radford A., Aramayo R., Natvig D.O., Alex L.A., Mannhaupt G., Ebbole D.J., Freitag M., Paulsen I., Sachs M.S., Lander E.S., Nusbaum C., Birren B.W.
    Nature 422:859-868(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987.
  3. "The structure of Neurospora crassa 3-carboxy-cis,cis-muconate lactonizing enzyme, a beta propeller cycloisomerase."
    Kajander T., Merckel M.C., Thompson A., Deacon A.M., Mazur P., Kozarich J.W., Goldman A.
    Structure 10:483-492(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS).

Entry informationi

Entry nameiCMLE_NEUCR
AccessioniPrimary (citable) accession number: P38677
Secondary accession number(s): Q7RV80
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: January 23, 2007
Last modified: June 24, 2015
This is version 99 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.