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P38677

- CMLE_NEUCR

UniProt

P38677 - CMLE_NEUCR

Protein

Carboxy-cis,cis-muconate cyclase

Gene

NCU04071

Organism
Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 94 (01 Oct 2014)
      Sequence version 3 (23 Jan 2007)
      Previous versions | rss
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    Functioni

    Catalyzes a syn cycloisomerization. Also possesses mle activity.

    Catalytic activityi

    3-carboxy-2,5-dihydro-5-oxofuran-2-acetate = 3-carboxy-cis,cis-muconate.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei149 – 1491
    Active sitei197 – 1971
    Active sitei213 – 2131
    Active sitei275 – 2751

    GO - Molecular functioni

    1. carboxy-cis,cis-muconate cyclase activity Source: UniProtKB-EC

    GO - Biological processi

    1. beta-ketoadipate pathway Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Isomerase

    Enzyme and pathway databases

    UniPathwayiUPA00157; UER00308.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Carboxy-cis,cis-muconate cyclase (EC:5.5.1.5)
    Alternative name(s):
    3-carboxy-cis,cis-muconate lactonizing enzyme
    Short name:
    CMLE
    Gene namesi
    ORF Names:NCU04071
    OrganismiNeurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987)
    Taxonomic identifieri367110 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaSordariomycetesSordariomycetidaeSordarialesSordariaceaeNeurospora
    ProteomesiUP000001805: Chromosome 5, Linkage Group VI

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11Removed1 Publication
    Chaini2 – 366365Carboxy-cis,cis-muconate cyclasePRO_0000171127Add
    BLAST

    Interactioni

    Subunit structurei

    Homotetramer.

    Protein-protein interaction databases

    STRINGi5141.NCU04071.1.

    Structurei

    Secondary structure

    1
    366
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi3 – 1412
    Beta strandi16 – 238
    Turni24 – 274
    Beta strandi28 – 369
    Beta strandi44 – 485
    Beta strandi52 – 609
    Beta strandi62 – 709
    Beta strandi73 – 819
    Helixi86 – 894
    Beta strandi96 – 1027
    Beta strandi110 – 1189
    Beta strandi121 – 1277
    Beta strandi133 – 1419
    Beta strandi148 – 1536
    Beta strandi157 – 1648
    Turni165 – 1684
    Beta strandi169 – 1757
    Beta strandi181 – 1888
    Beta strandi196 – 2016
    Beta strandi205 – 2128
    Turni213 – 2164
    Beta strandi217 – 2237
    Turni225 – 2273
    Beta strandi230 – 24011
    Turni249 – 2513
    Beta strandi252 – 26312
    Beta strandi267 – 27812
    Beta strandi284 – 2907
    Beta strandi296 – 3049
    Beta strandi315 – 3173
    Beta strandi324 – 3285
    Beta strandi330 – 3323
    Beta strandi334 – 3418
    Beta strandi344 – 3529
    Beta strandi358 – 3658

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1JOFX-ray2.50A/B/C/D/E/F/G/H2-366[»]
    ProteinModelPortaliP38677.
    SMRiP38677. Positions 2-366.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP38677.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the cycloisomerase 2 family.Curated

    Phylogenomic databases

    eggNOGiNOG133463.
    HOGENOMiHOG000216586.
    KOiK14334.
    OMAiTPPGAIF.
    OrthoDBiEOG7VB2QX.

    Family and domain databases

    Gene3Di2.130.10.10. 1 hit.
    InterProiIPR019405. Lactonase_7-beta_prop.
    IPR015943. WD40/YVTN_repeat-like_dom.
    [Graphical view]
    PfamiPF10282. Lactonase. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P38677-1 [UniParc]FASTAAdd to Basket

    « Hide

    MPLHHLMIGT WTPPGAIFTV QFDDEKLTCK LIKRTEIPQD EPISWMTFDH    50
    ERKNIYGAAM KKWSSFAVKS PTEIVHEASH PIGGHPRAND ADTNTRAIFL 100
    LAAKQPPYAV YANPFYKFAG YGNVFSVSET GKLEKNVQNY EYQENTGIHG 150
    MVFDPTETYL YSADLTANKL WTHRKLASGE VELVGSVDAP DPGDHPRWVA 200
    MHPTGNYLYA LMEAGNRICE YVIDPATHMP VYTHHSFPLI PPGIPDRDPE 250
    TGKGLYRADV CALTFSGKYM FASSRANKFE LQGYIAGFKL RDCGSIEKQL 300
    FLSPTPTSGG HSNAVSPCPW SDEWMAITDD QEGWLEIYRW KDEFLHRVAR 350
    VRIPEPGFGM NAIWYD 366
    Length:366
    Mass (Da):41,329
    Last modified:January 23, 2007 - v3
    Checksum:iE71BA50509E72873
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L27538 mRNA. Translation: AAA21020.1.
    CM002241 Genomic DNA. Translation: EAA28450.1.
    PIRiA55525.
    RefSeqiXP_957686.1. XM_952593.1.

    Genome annotation databases

    EnsemblFungiiEFNCRT00000003530; EFNCRP00000003530; EFNCRG00000003526.
    GeneIDi3873776.
    KEGGincr:NCU04071.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L27538 mRNA. Translation: AAA21020.1 .
    CM002241 Genomic DNA. Translation: EAA28450.1 .
    PIRi A55525.
    RefSeqi XP_957686.1. XM_952593.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1JOF X-ray 2.50 A/B/C/D/E/F/G/H 2-366 [» ]
    ProteinModelPortali P38677.
    SMRi P38677. Positions 2-366.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 5141.NCU04071.1.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii EFNCRT00000003530 ; EFNCRP00000003530 ; EFNCRG00000003526 .
    GeneIDi 3873776.
    KEGGi ncr:NCU04071.

    Phylogenomic databases

    eggNOGi NOG133463.
    HOGENOMi HOG000216586.
    KOi K14334.
    OMAi TPPGAIF.
    OrthoDBi EOG7VB2QX.

    Enzyme and pathway databases

    UniPathwayi UPA00157 ; UER00308 .

    Miscellaneous databases

    EvolutionaryTracei P38677.

    Family and domain databases

    Gene3Di 2.130.10.10. 1 hit.
    InterProi IPR019405. Lactonase_7-beta_prop.
    IPR015943. WD40/YVTN_repeat-like_dom.
    [Graphical view ]
    Pfami PF10282. Lactonase. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "3-carboxy-cis,cis-muconate lactonizing enzyme from Neurospora crassa: an alternate cycloisomerase motif."
      Mazur P., Henzel W.J., Mattoo S., Kozarich J.W.
      J. Bacteriol. 176:1718-1728(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 2-41, CHARACTERIZATION.
      Strain: ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987.
    2. "The genome sequence of the filamentous fungus Neurospora crassa."
      Galagan J.E., Calvo S.E., Borkovich K.A., Selker E.U., Read N.D., Jaffe D.B., FitzHugh W., Ma L.-J., Smirnov S., Purcell S., Rehman B., Elkins T., Engels R., Wang S., Nielsen C.B., Butler J., Endrizzi M., Qui D.
      , Ianakiev P., Bell-Pedersen D., Nelson M.A., Werner-Washburne M., Selitrennikoff C.P., Kinsey J.A., Braun E.L., Zelter A., Schulte U., Kothe G.O., Jedd G., Mewes H.-W., Staben C., Marcotte E., Greenberg D., Roy A., Foley K., Naylor J., Stange-Thomann N., Barrett R., Gnerre S., Kamal M., Kamvysselis M., Mauceli E.W., Bielke C., Rudd S., Frishman D., Krystofova S., Rasmussen C., Metzenberg R.L., Perkins D.D., Kroken S., Cogoni C., Macino G., Catcheside D.E.A., Li W., Pratt R.J., Osmani S.A., DeSouza C.P.C., Glass N.L., Orbach M.J., Berglund J.A., Voelker R., Yarden O., Plamann M., Seiler S., Dunlap J.C., Radford A., Aramayo R., Natvig D.O., Alex L.A., Mannhaupt G., Ebbole D.J., Freitag M., Paulsen I., Sachs M.S., Lander E.S., Nusbaum C., Birren B.W.
      Nature 422:859-868(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987.
    3. "The structure of Neurospora crassa 3-carboxy-cis,cis-muconate lactonizing enzyme, a beta propeller cycloisomerase."
      Kajander T., Merckel M.C., Thompson A., Deacon A.M., Mazur P., Kozarich J.W., Goldman A.
      Structure 10:483-492(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS).

    Entry informationi

    Entry nameiCMLE_NEUCR
    AccessioniPrimary (citable) accession number: P38677
    Secondary accession number(s): Q7RV80
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1995
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 94 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3