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P38677 (CMLE_NEUCR) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 93. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Carboxy-cis,cis-muconate cyclase

EC=5.5.1.5
Alternative name(s):
3-carboxy-cis,cis-muconate lactonizing enzyme
Short name=CMLE
Gene names
ORF Names:NCU04071
OrganismNeurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987) [Reference proteome]
Taxonomic identifier367110 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaPezizomycotinaSordariomycetesSordariomycetidaeSordarialesSordariaceaeNeurospora

Protein attributes

Sequence length366 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Catalyzes a syn cycloisomerization. Also possesses mle activity.

Catalytic activity

3-carboxy-2,5-dihydro-5-oxofuran-2-acetate = 3-carboxy-cis,cis-muconate.

Pathway

Aromatic compound metabolism; beta-ketoadipate pathway; 3-carboxy-cis,cis-muconate from 3-carboxy-2,5-dihydro-5-oxofuran-2-acetate: step 1/1.

Subunit structure

Homotetramer.

Sequence similarities

Belongs to the cycloisomerase 2 family.

Ontologies

Keywords
   Molecular functionIsomerase
   Technical term3D-structure
Complete proteome
Direct protein sequencing
Reference proteome
Gene Ontology (GO)
   Biological_processbeta-ketoadipate pathway

Inferred from electronic annotation. Source: UniProtKB-UniPathway

   Molecular_functioncarboxy-cis,cis-muconate cyclase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed Ref.1
Chain2 – 366365Carboxy-cis,cis-muconate cyclase
PRO_0000171127

Sites

Active site1491
Active site1971
Active site2131
Active site2751

Secondary structure

................................................................ 366
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P38677 [UniParc].

Last modified January 23, 2007. Version 3.
Checksum: E71BA50509E72873

FASTA36641,329
        10         20         30         40         50         60 
MPLHHLMIGT WTPPGAIFTV QFDDEKLTCK LIKRTEIPQD EPISWMTFDH ERKNIYGAAM 

        70         80         90        100        110        120 
KKWSSFAVKS PTEIVHEASH PIGGHPRAND ADTNTRAIFL LAAKQPPYAV YANPFYKFAG 

       130        140        150        160        170        180 
YGNVFSVSET GKLEKNVQNY EYQENTGIHG MVFDPTETYL YSADLTANKL WTHRKLASGE 

       190        200        210        220        230        240 
VELVGSVDAP DPGDHPRWVA MHPTGNYLYA LMEAGNRICE YVIDPATHMP VYTHHSFPLI 

       250        260        270        280        290        300 
PPGIPDRDPE TGKGLYRADV CALTFSGKYM FASSRANKFE LQGYIAGFKL RDCGSIEKQL 

       310        320        330        340        350        360 
FLSPTPTSGG HSNAVSPCPW SDEWMAITDD QEGWLEIYRW KDEFLHRVAR VRIPEPGFGM 


NAIWYD 

« Hide

References

« Hide 'large scale' references
[1]"3-carboxy-cis,cis-muconate lactonizing enzyme from Neurospora crassa: an alternate cycloisomerase motif."
Mazur P., Henzel W.J., Mattoo S., Kozarich J.W.
J. Bacteriol. 176:1718-1728(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 2-41, CHARACTERIZATION.
Strain: ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987.
[2]"The genome sequence of the filamentous fungus Neurospora crassa."
Galagan J.E., Calvo S.E., Borkovich K.A., Selker E.U., Read N.D., Jaffe D.B., FitzHugh W., Ma L.-J., Smirnov S., Purcell S., Rehman B., Elkins T., Engels R., Wang S., Nielsen C.B., Butler J., Endrizzi M., Qui D. expand/collapse author list , Ianakiev P., Bell-Pedersen D., Nelson M.A., Werner-Washburne M., Selitrennikoff C.P., Kinsey J.A., Braun E.L., Zelter A., Schulte U., Kothe G.O., Jedd G., Mewes H.-W., Staben C., Marcotte E., Greenberg D., Roy A., Foley K., Naylor J., Stange-Thomann N., Barrett R., Gnerre S., Kamal M., Kamvysselis M., Mauceli E.W., Bielke C., Rudd S., Frishman D., Krystofova S., Rasmussen C., Metzenberg R.L., Perkins D.D., Kroken S., Cogoni C., Macino G., Catcheside D.E.A., Li W., Pratt R.J., Osmani S.A., DeSouza C.P.C., Glass N.L., Orbach M.J., Berglund J.A., Voelker R., Yarden O., Plamann M., Seiler S., Dunlap J.C., Radford A., Aramayo R., Natvig D.O., Alex L.A., Mannhaupt G., Ebbole D.J., Freitag M., Paulsen I., Sachs M.S., Lander E.S., Nusbaum C., Birren B.W.
Nature 422:859-868(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987.
[3]"The structure of Neurospora crassa 3-carboxy-cis,cis-muconate lactonizing enzyme, a beta propeller cycloisomerase."
Kajander T., Merckel M.C., Thompson A., Deacon A.M., Mazur P., Kozarich J.W., Goldman A.
Structure 10:483-492(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS).

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
L27538 mRNA. Translation: AAA21020.1.
CM002241 Genomic DNA. Translation: EAA28450.1.
PIRA55525.
RefSeqXP_957686.1. XM_952593.1.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1JOFX-ray2.50A/B/C/D/E/F/G/H2-366[»]
ProteinModelPortalP38677.
SMRP38677. Positions 2-366.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING5141.NCU04071.1.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiEFNCRT00000003530; EFNCRP00000003530; EFNCRG00000003526.
GeneID3873776.
KEGGncr:NCU04071.

Phylogenomic databases

eggNOGNOG133463.
HOGENOMHOG000216586.
KOK14334.
OMATPPGAIF.
OrthoDBEOG7VB2QX.

Enzyme and pathway databases

UniPathwayUPA00157; UER00308.

Family and domain databases

Gene3D2.130.10.10. 1 hit.
InterProIPR019405. Lactonase_7-beta_prop.
IPR015943. WD40/YVTN_repeat-like_dom.
[Graphical view]
PfamPF10282. Lactonase. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceP38677.

Entry information

Entry nameCMLE_NEUCR
AccessionPrimary (citable) accession number: P38677
Secondary accession number(s): Q7RV80
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: January 23, 2007
Last modified: February 19, 2014
This is version 93 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

PATHWAY comments

Index of metabolic and biosynthesis pathways