Reviewed, UniProtKB/Swiss-Prot P38675 (MET7_NEUCR)

Names and origin

Protein names

Recommended name:
    Cystathionine gamma-synthase
    EC=2.5.1.48
Alternative name(s):
    O-succinylhomoserine (thiol)-lyase

Gene names

Name:	met-7
ORF Names:	NCU02430

Organism

Neurospora crassa [Complete proteome]

Taxonomic identifier

5141 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Pezizomycotina › Sordariomycetes › Sordariomycetidae › Sordariales › Sordariaceae › Neurospora

Protein attributes

Sequence length	621 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	O(4)-succinyl-L-homoserine + L-cysteine = L-cystathionine + succinate.
Cofactor	Pyridoxal phosphate.
Pathway	Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway.
Subunit structure	Both met-3 and met-7 are required to form a functional cystathionine gamma-synthase.
Sequence similarities	Belongs to the trans-sulfuration enzymes family. MET7 subfamily.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Methionine biosynthesis
Ligand	Pyridoxal phosphate
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	methionine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	cystathionine gamma-synthase activity Inferred from electronic annotation. Source: EC pyridoxal phosphate binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 621	621	Cystathionine gamma-synthase		PRO_0000114779
Amino acid modifications
	Modified residue	429	1	N6-(pyridoxal phosphate)lysine Potential
Experimental info
	Sequence conflict	194	1	R → A Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P38675-1 [UniParc]. Last modified December 4, 2007. Version 3. Checksum: 7A3B6166218B162A	FASTA	621	68,938
	10 20 30 40 50 60 MPPFELGDPI PSETAHAVSV SLPTWSANVG YEEGQDWVVK RMATGYPRFF IHRTIQAFAA 70 80 90 100 110 120 DIVATHVSTK AKRTSDITAM LFPTPTIASR CVDFIRSRAP ADVCSNIEVV NLVLDMSDPE 130 140 150 160 170 180 ARALEPLCPS ISAVIMPQDG FPFAKQYWQH SGDGVSSRRA EFCHGLFKDG LLRPDTELRN 190 200 210 220 230 240 AAVSAAKPCR GPKRYQRQAS LDAGGNQQTI MHGHIHRATG ETAMIQETSR FLEERFGRNL 250 260 270 280 290 300 DLSFVHPAKS AIKRRIAGAL RSADHDLGGS PSLSEKQMSS NTRGIANLRE EDIYLFPCGM 310 320 330 340 350 360 NAIFHAHRAL YSIRTPPGST PLKSVNFGFP YVDTLKILEK FNPSGALFYG HGSKSDLDDL 370 380 390 400 410 420 ETRLESGERY LALFCEFPGN PLLTCPDLVR IRELADKYEF AVVVDETIGT FANVNVLQFA 430 440 450 460 470 480 DIVVSSLTKI FSGDCNVMGG GAIFNPNSRY YSALKSFVQQ QLEDTYWPED VIFMERNSRD 490 500 510 520 530 540 FVARIDRVNA NAEAICRVLQ DHPLVKTLYY PKYNDSRANY EAVKLPQGGY GGLISVVLKG 550 560 570 580 590 600 KKQAVAFYDA IETAKGPSLG TNFTLTSPYV LLAHYQELDW AEQYGVDRNL IRISVGLEGT 610 620 DELINVFTRA LKVAEEQSQY P

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The genome sequence of the filamentous fungus Neurospora crassa." Galagan J.E., Calvo S.E., Borkovich K.A., Selker E.U., Read N.D., Jaffe D.B., FitzHugh W., Ma L.-J., Smirnov S., Purcell S., Rehman B., Elkins T., Engels R., Wang S., Nielsen C.B., Butler J., Endrizzi M., Qui D. , Ianakiev P., Bell-Pedersen D., Nelson M.A., Werner-Washburne M., Selitrennikoff C.P., Kinsey J.A., Braun E.L., Zelter A., Schulte U., Kothe G.O., Jedd G., Mewes H.-W., Staben C., Marcotte E., Greenberg D., Roy A., Foley K., Naylor J., Stange-Thomann N., Barrett R., Gnerre S., Kamal M., Kamvysselis M., Mauceli E.W., Bielke C., Rudd S., Frishman D., Krystofova S., Rasmussen C., Metzenberg R.L., Perkins D.D., Kroken S., Cogoni C., Macino G., Catcheside D.E.A., Li W., Pratt R.J., Osmani S.A., DeSouza C.P.C., Glass N.L., Orbach M.J., Berglund J.A., Voelker R., Yarden O., Plamann M., Seiler S., Dunlap J.C., Radford A., Aramayo R., Natvig D.O., Alex L.A., Mannhaupt G., Ebbole D.J., Freitag M., Paulsen I., Sachs M.S., Lander E.S., Nusbaum C., Birren B.W. Nature 422:859-868(2003) [PubMed: 12712197] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987.
[2]	"Sequence and characterization of the met-7 gene of Neurospora crassa." Crawford J.M., Geever R.F., Asch D.K., Case M.E. Gene 111:265-266(1992) [PubMed: 1531800] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 49-621.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AABX02000010 Genomic DNA. Translation: EAA30199.2. M64066 Genomic DNA. No translation available.
PIR	JQ1524.
RefSeq	XP_959435.2.
3D structure databases
HSSP	HSSP built from PDB template 1GC0 based on UniProtKB P13254.
ModBase	Search...
Protein-protein interaction databases
STRING	P38675.
Genome annotation databases
GeneID	3875582.
KEGG	ncr:NCU02430.
NMPDR	fig|5141.1.peg.9139.
Enzyme and pathway databases
BioCyc	NCRA-XX3-01:NCRA-XX3-01-006178-MON.
BRENDA	2.5.1.48. 266.
Family and domain databases
InterPro	IPR000277. Cys/Met-Metab_PyrdxlP-dep_enz. IPR015421. PyrdxlP-dep_Trfase_major_sub1. IPR015422. PyrdxlP-dep_Trfase_major_sub2. [Graphical view]
Gene3D	G3DSA:3.40.640.10. PyrdxlP-dep_Trfase_major_sub1. 2 hits. G3DSA:3.90.1150.10. PyrdxlP-dep_Trfase_major_sub2. 2 hits.
PANTHER	PTHR11808. Cys_Met_Meta_PP. 2 hits.
Pfam	PF01053. Cys_Met_Meta_PP. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MET7_NEUCR

Accession

Primary (citable) accession number: P38675
Secondary accession number(s): Q7RV94

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	December 4, 2007
Last modified:	September 1, 2009
This is version 63 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)