Reviewed, UniProtKB/Swiss-Prot P38674 (ILV5_NEUCR)

Names and origin

Protein names

Recommended name:
    Ketol-acid reductoisomerase, mitochondrial
    EC=1.1.1.86
Alternative name(s):
    Acetohydroxy-acid reductoisomerase
    Alpha-keto-beta-hydroxylacil reductoisomerase

Gene names

Name:	ilv-2
ORF Names:	B11H24.150, NCU03608

Organism

Neurospora crassa [Complete proteome]

Taxonomic identifier

5141 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Pezizomycotina › Sordariomycetes › Sordariomycetidae › Sordariales › Sordariaceae › Neurospora

Protein attributes

Sequence length	402 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	(R)-2,3-dihydroxy-3-methylbutanoate + NADP+ = (S)-2-hydroxy-2-methyl-3-oxobutanoate + NADPH. (2R,3R)-2,3-dihydroxy-3-methylpentanoate + NADP+ = (S)-2-hydroxy-2-ethyl-3-oxobutanoate + NADPH.
Cofactor	Magnesium.
Pathway	Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 2/4. Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 2/4.
Subcellular location	Mitochondrion.
Sequence similarities	Belongs to the ketol-acid reductoisomerase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Branched-chain amino acid biosynthesis
Cellular component	Mitochondrion
Domain	Transit peptide
Ligand	Magnesium NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	branched chain family amino acid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	mitochondrion Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	coenzyme binding Inferred from electronic annotation. Source: InterPro ketol-acid reductoisomerase activity Inferred from electronic annotation. Source: EC magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 26	26	Mitochondrion Potential
	Chain	27 – 402	376	Ketol-acid reductoisomerase, mitochondrial		PRO_0000015632
Regions
	Nucleotide binding	90 – 99	10	NADP Potential
Sites
	Active site	177	1	Potential
Experimental info
	Sequence conflict	358	1	T → R in AAB00797. Ref.1
	Sequence conflict	392 – 393	2	Missing in AAB00797. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P38674-1 [UniParc]. Last modified April 30, 2003. Version 2. Checksum: 246F795898D2A174	FASTA	402	44,623
	10 20 30 40 50 60 MAARNCTKAL RPLARQLATP AVQRRTFVAA ASAVRASVAV KAVAAPARQQ VRGVKTMDFA 70 80 90 100 110 120 GHKEEVHERA DWPAEKLLDY FKNDTLALIG YGSQGHGQGL NLRDNGLNVI VGVRKNGKSW 130 140 150 160 170 180 EDAIQDGWVP GKNLFDVDEA ISRGTIVMNL LSDAAQSETW PHIKPQITKG KTLYFSHGFS 190 200 210 220 230 240 PVFKDLTKVE VPTDVDVILV APKGSGRTVR SLFREGRGIN SSFAVYQDVT GKAKEKAVAL 250 260 270 280 290 300 GVAVGSGYLY ETTFEKEVYS DLYGERGCLM GGIHGMFLAQ YEVLRERGHS PSEAFNETVE 310 320 330 340 350 360 EATQSLYPLI GAHGMDWMFD ACSTTARRGA IDWTPKFKDA LKPVFNNLYD SVKNGDETKR 370 380 390 400 SLEYNSQPDY RERYEAELDE IRNLEIWRAG KAVRSLRPEN QK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Characterization of the ilv-2 gene from Neurospora crassa encoding alpha-keto-beta-hydroxylacyl reductoisomerase." Sista H., Bowman B. Gene 120:115-118(1992) [PubMed: 1398116] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"What's in the genome of a filamentous fungus? Analysis of the Neurospora genome sequence." Mannhaupt G., Montrone C., Haase D., Mewes H.-W., Aign V., Hoheisel J.D., Fartmann B., Nyakatura G., Kempken F., Maier J., Schulte U. Nucleic Acids Res. 31:1944-1954(2003) [PubMed: 12655011] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987.
[3]	"The genome sequence of the filamentous fungus Neurospora crassa." Galagan J.E., Calvo S.E., Borkovich K.A., Selker E.U., Read N.D., Jaffe D.B., FitzHugh W., Ma L.-J., Smirnov S., Purcell S., Rehman B., Elkins T., Engels R., Wang S., Nielsen C.B., Butler J., Endrizzi M., Qui D. , Ianakiev P., Bell-Pedersen D., Nelson M.A., Werner-Washburne M., Selitrennikoff C.P., Kinsey J.A., Braun E.L., Zelter A., Schulte U., Kothe G.O., Jedd G., Mewes H.-W., Staben C., Marcotte E., Greenberg D., Roy A., Foley K., Naylor J., Stange-Thomann N., Barrett R., Gnerre S., Kamal M., Kamvysselis M., Mauceli E.W., Bielke C., Rudd S., Frishman D., Krystofova S., Rasmussen C., Metzenberg R.L., Perkins D.D., Kroken S., Cogoni C., Macino G., Catcheside D.E.A., Li W., Pratt R.J., Osmani S.A., DeSouza C.P.C., Glass N.L., Orbach M.J., Berglund J.A., Voelker R., Yarden O., Plamann M., Seiler S., Dunlap J.C., Radford A., Aramayo R., Natvig D.O., Alex L.A., Mannhaupt G., Ebbole D.J., Freitag M., Paulsen I., Sachs M.S., Lander E.S., Nusbaum C., Birren B.W. Nature 422:859-868(2003) [PubMed: 12712197] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987.

Cross-references

Sequence databases
EMBLGenBankDDBJ	M84189 Genomic DNA. Translation: AAB00797.1. AL670005 Genomic DNA. Translation: CAD21284.1. AABX02000011 Genomic DNA. Translation: EAA32099.1.
PIR	JC1428.
RefSeq	XP_961335.1.
3D structure databases
HSSP	HSSP built from PDB template 1NP3 based on UniProtKB Q9HVA2.
ModBase	Search...
Genome annotation databases
GeneID	3877517.
KEGG	ncr:NCU03608.
NMPDR	fig|5141.1.peg.843.
Enzyme and pathway databases
BioCyc	NCRA-XX3-01:NCRA-XX3-01-007356-MON.
BRENDA	1.1.1.86. 266.
Family and domain databases
InterPro	IPR013023. AcH_isomrdctse. IPR000506. AcH_isomrdctse_C. IPR013328. DH_multihelical. IPR013116. IlvN. IPR016207. KetolA_reductoisomerase_fun. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit. G3DSA:1.10.1040.10. Opine_DH. 1 hit.
PANTHER	PTHR21371. AcH_isomrdctse. 1 hit.
Pfam	PF01450. IlvC. 1 hit. PF07991. IlvN. 1 hit. [Graphical view]
PIRSF	PIRSF000119. Ilv5_fungal. 1 hit.
TIGRFAMs	TIGR00465. ilvC. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ILV5_NEUCR

Accession

Primary (citable) accession number: P38674
Secondary accession number(s): Q7RVD5, Q8X0I9

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	April 30, 2003
Last modified:	June 16, 2009
This is version 75 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)