ID   ERG24_NEUCR             Reviewed;         490 AA.
AC   P38670; Q7RV50;
DT   01-FEB-1995, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1995, sequence version 1.
DT   03-MAR-2009, entry version 67.
DE   RecName: Full=Delta(14)-sterol reductase;
DE            EC=1.3.1.70;
DE   AltName: Full=C-14 sterol reductase;
DE   AltName: Full=Sterol C14-reductase;
GN   Name=erg-3; Synonyms=erg3; ORFNames=B2G14.010, NCU08762;
OS   Neurospora crassa.
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina;
OC   Sordariomycetes; Sordariomycetidae; Sordariales; Sordariaceae;
OC   Neurospora.
OX   NCBI_TaxID=5141;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987;
RA   Papavinasasundaram K.G., Kasbekar D.P.;
RT   "The Neurospora crassa erg3 gene encodes a protein with sequence
RT   homology to both yeast sterol C-14 reductase and chicken lamin B
RT   receptor.";
RL   J. Genet. 73:33-41(1994).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987;
RX   MEDLINE=22542210; PubMed=12655011; DOI=10.1093/nar/gkg293;
RA   Mannhaupt G., Montrone C., Haase D., Mewes H.-W., Aign V.,
RA   Hoheisel J.D., Fartmann B., Nyakatura G., Kempken F., Maier J.,
RA   Schulte U.;
RT   "What's in the genome of a filamentous fungus? Analysis of the
RT   Neurospora genome sequence.";
RL   Nucleic Acids Res. 31:1944-1954(2003).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987;
RX   MEDLINE=22598136; PubMed=12712197; DOI=10.1038/nature01554;
RA   Galagan J.E., Calvo S.E., Borkovich K.A., Selker E.U., Read N.D.,
RA   Jaffe D.B., FitzHugh W., Ma L.-J., Smirnov S., Purcell S., Rehman B.,
RA   Elkins T., Engels R., Wang S., Nielsen C.B., Butler J., Endrizzi M.,
RA   Qui D., Ianakiev P., Bell-Pedersen D., Nelson M.A.,
RA   Werner-Washburne M., Selitrennikoff C.P., Kinsey J.A., Braun E.L.,
RA   Zelter A., Schulte U., Kothe G.O., Jedd G., Mewes H.-W., Staben C.,
RA   Marcotte E., Greenberg D., Roy A., Foley K., Naylor J.,
RA   Stange-Thomann N., Barrett R., Gnerre S., Kamal M., Kamvysselis M.,
RA   Mauceli E.W., Bielke C., Rudd S., Frishman D., Krystofova S.,
RA   Rasmussen C., Metzenberg R.L., Perkins D.D., Kroken S., Cogoni C.,
RA   Macino G., Catcheside D.E.A., Li W., Pratt R.J., Osmani S.A.,
RA   DeSouza C.P.C., Glass N.L., Orbach M.J., Berglund J.A., Voelker R.,
RA   Yarden O., Plamann M., Seiler S., Dunlap J.C., Radford A., Aramayo R.,
RA   Natvig D.O., Alex L.A., Mannhaupt G., Ebbole D.J., Freitag M.,
RA   Paulsen I., Sachs M.S., Lander E.S., Nusbaum C., Birren B.W.;
RT   "The genome sequence of the filamentous fungus Neurospora crassa.";
RL   Nature 422:859-868(2003).
CC   -!- FUNCTION: Reduces the C14=C15 double bond of 4,4-dimethyl-
CC       cholesta-8,14,24-trienol to produce 4,4-dimethyl-cholesta-8,24-
CC       dienol.
CC   -!- CATALYTIC ACTIVITY: 4,4-dimethyl-5-alpha-cholesta-8,24-dien-3-
CC       beta-ol + NADP(+) = 4,4-dimethyl-5-alpha-cholesta-8,14,24-trien-3-
CC       beta-ol + NADPH.
CC   -!- PATHWAY: Steroid biosynthesis; zymosterol biosynthesis; zymosterol
CC       from lanosterol: step 2/6.
CC   -!- SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein
CC       (Probable).
CC   -!- SIMILARITY: Belongs to the ERG4/ERG24 family.
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DR   EMBL; X77955; CAA54919.1; -; Genomic_DNA.
DR   EMBL; BX284753; CAD70446.1; -; Genomic_DNA.
DR   EMBL; AABX02000017; EAA26875.1; -; Genomic_DNA.
DR   PIR; S44170; S44170.
DR   RefSeq; XP_956111.1; -.
DR   GeneID; 3872249; -.
DR   KEGG; ncr:NCU08762; -.
DR   NMPDR; fig|5141.1.peg.8812; -.
DR   BioCyc; NCRA-XX3-01:NCRA-XX3-01-003621-MON; -.
DR   BRENDA; 1.3.1.70; 266.
DR   GO; GO:0016021; C:integral to membrane; IEA:UniProtKB-KW.
DR   GO; GO:0050613; F:delta14-sterol reductase activity; IEA:EC.
DR   GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW.
DR   GO; GO:0016126; P:sterol biosynthetic process; IEA:UniProtKB-KW.
DR   InterPro; IPR001171; Ergosterol_biosynth_ERG4_ERG24.
DR   InterPro; IPR018083; Sterol_reductase_CS.
DR   Pfam; PF01222; ERG4_ERG24; 1.
DR   PROSITE; PS01017; STEROL_REDUCT_1; 1.
DR   PROSITE; PS01018; STEROL_REDUCT_2; 1.
PE   3: Inferred from homology;
KW   Complete proteome; Lipid synthesis; Membrane; NADP; Oxidoreductase;
KW   Steroid biosynthesis; Sterol biosynthesis; Transmembrane.
FT   CHAIN         1    490       Delta(14)-sterol reductase.
FT                                /FTId=PRO_0000207497.
FT   TRANSMEM     23     43       Potential.
FT   TRANSMEM     80    100       Potential.
FT   TRANSMEM    136    156       Potential.
FT   TRANSMEM    160    180       Potential.
FT   TRANSMEM    230    250       Potential.
FT   TRANSMEM    255    275       Potential.
FT   TRANSMEM    324    344       Potential.
FT   TRANSMEM    436    456       Potential.
SQ   SEQUENCE   490 AA;  54722 MW;  9CF3104EF3014085 CRC64;
     MAGKQNQAAP KKAVAPRQQH YEFGGPIGAF GITFGLPILV HVFNLFCNDI SGCPAPSLLH
     PKSLDLAQLK REIGWPDNGV FGLFSWSATL WTLGYYALSL VQYRFLPGHH VEGTELSTGG
     RLKYKLNAFN SAMCTLAILA AGTIAQGAEF PVWTFISDNF AQIISANILF AFALAIFVYV
     RSFDVKPGNK DMRQLAAGGV TGSLIYDFYI GRELNPRITL PLIGQVDIKE FMEMRPGLLG
     WIILNCAFIA KQYRLYGYVT DSILFITAIQ AFYVFDGIYM EPAVLTTMDI TTDGFGFMLS
     FGDVVWVPFM YSTQTRYLSV HPQQLGAFGL IAVGAVLAAG YSIFRLSNSQ KNNFRTNPED
     PSVKHLTYLQ TKTGSRLITS GWWGIARHIN YLGDWLQSWP YSLPTGIAGY QILSAGSNAP
     GAITMLDGRE VVQGEARGWG IVFTYFYILY FAILLIHRDL RDDEKCSKKY GDDWEKYKKL
     VKWRIVPGIY
//