Reviewed, UniProtKB/Swiss-Prot P38670 (ERG24_NEUCR)

Names and origin

Protein names

Recommended name:
    Delta(14)-sterol reductase
    EC=1.3.1.70
Alternative name(s):
    C-14 sterol reductase
    Sterol C14-reductase

Gene names

Name:	erg-3
Synonyms:	erg3
ORF Names:	B2G14.010, NCU08762

Organism

Neurospora crassa [Complete proteome]

Taxonomic identifier

5141 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Pezizomycotina › Sordariomycetes › Sordariomycetidae › Sordariales › Sordariaceae › Neurospora

Protein attributes

Sequence length	490 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Reduces the C14=C15 double bond of 4,4-dimethyl-cholesta-8,14,24-trienol to produce 4,4-dimethyl-cholesta-8,24-dienol.
Catalytic activity	4,4-dimethyl-5-alpha-cholesta-8,24-dien-3-beta-ol + NADP+ = 4,4-dimethyl-5-alpha-cholesta-8,14,24-trien-3-beta-ol + NADPH.
Pathway	Steroid biosynthesis; zymosterol biosynthesis; zymosterol from lanosterol: step 2/6.
Subcellular location	Membrane; Multi-pass membrane protein Probable.
Sequence similarities	Belongs to the ERG4/ERG24 family.

Ontologies

Keywords
Biological process	Lipid synthesis Steroid biosynthesis Sterol biosynthesis
Cellular component	Membrane
Domain	Transmembrane
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW sterol biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	delta14-sterol reductase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 490	490	Delta(14)-sterol reductase		PRO_0000207497
Regions
	Transmembrane	23 – 43	21	Potential
	Transmembrane	80 – 100	21	Potential
	Transmembrane	136 – 156	21	Potential
	Transmembrane	160 – 180	21	Potential
	Transmembrane	230 – 250	21	Potential
	Transmembrane	255 – 275	21	Potential
	Transmembrane	324 – 344	21	Potential
	Transmembrane	436 – 456	21	Potential

Sequences

	Sequence		Length	Mass (Da)
	P38670-1 [UniParc]. Last modified February 1, 1995. Version 1. Checksum: 9CF3104EF3014085	FASTA	490	54,722
	10 20 30 40 50 60 MAGKQNQAAP KKAVAPRQQH YEFGGPIGAF GITFGLPILV HVFNLFCNDI SGCPAPSLLH 70 80 90 100 110 120 PKSLDLAQLK REIGWPDNGV FGLFSWSATL WTLGYYALSL VQYRFLPGHH VEGTELSTGG 130 140 150 160 170 180 RLKYKLNAFN SAMCTLAILA AGTIAQGAEF PVWTFISDNF AQIISANILF AFALAIFVYV 190 200 210 220 230 240 RSFDVKPGNK DMRQLAAGGV TGSLIYDFYI GRELNPRITL PLIGQVDIKE FMEMRPGLLG 250 260 270 280 290 300 WIILNCAFIA KQYRLYGYVT DSILFITAIQ AFYVFDGIYM EPAVLTTMDI TTDGFGFMLS 310 320 330 340 350 360 FGDVVWVPFM YSTQTRYLSV HPQQLGAFGL IAVGAVLAAG YSIFRLSNSQ KNNFRTNPED 370 380 390 400 410 420 PSVKHLTYLQ TKTGSRLITS GWWGIARHIN YLGDWLQSWP YSLPTGIAGY QILSAGSNAP 430 440 450 460 470 480 GAITMLDGRE VVQGEARGWG IVFTYFYILY FAILLIHRDL RDDEKCSKKY GDDWEKYKKL 490 VKWRIVPGIY

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The Neurospora crassa erg3 gene encodes a protein with sequence homology to both yeast sterol C-14 reductase and chicken lamin B receptor." Papavinasasundaram K.G., Kasbekar D.P. J. Genet. 73:33-41(1994) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987.
[2]	"What's in the genome of a filamentous fungus? Analysis of the Neurospora genome sequence." Mannhaupt G., Montrone C., Haase D., Mewes H.-W., Aign V., Hoheisel J.D., Fartmann B., Nyakatura G., Kempken F., Maier J., Schulte U. Nucleic Acids Res. 31:1944-1954(2003) [PubMed: 12655011] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987.
[3]	"The genome sequence of the filamentous fungus Neurospora crassa." Galagan J.E., Calvo S.E., Borkovich K.A., Selker E.U., Read N.D., Jaffe D.B., FitzHugh W., Ma L.-J., Smirnov S., Purcell S., Rehman B., Elkins T., Engels R., Wang S., Nielsen C.B., Butler J., Endrizzi M., Qui D. , Ianakiev P., Bell-Pedersen D., Nelson M.A., Werner-Washburne M., Selitrennikoff C.P., Kinsey J.A., Braun E.L., Zelter A., Schulte U., Kothe G.O., Jedd G., Mewes H.-W., Staben C., Marcotte E., Greenberg D., Roy A., Foley K., Naylor J., Stange-Thomann N., Barrett R., Gnerre S., Kamal M., Kamvysselis M., Mauceli E.W., Bielke C., Rudd S., Frishman D., Krystofova S., Rasmussen C., Metzenberg R.L., Perkins D.D., Kroken S., Cogoni C., Macino G., Catcheside D.E.A., Li W., Pratt R.J., Osmani S.A., DeSouza C.P.C., Glass N.L., Orbach M.J., Berglund J.A., Voelker R., Yarden O., Plamann M., Seiler S., Dunlap J.C., Radford A., Aramayo R., Natvig D.O., Alex L.A., Mannhaupt G., Ebbole D.J., Freitag M., Paulsen I., Sachs M.S., Lander E.S., Nusbaum C., Birren B.W. Nature 422:859-868(2003) [PubMed: 12712197] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X77955 Genomic DNA. Translation: CAA54919.1. BX284753 Genomic DNA. Translation: CAD70446.1. AABX02000017 Genomic DNA. Translation: EAA26875.1.
PIR	S44170.
RefSeq	XP_956111.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3872249.
KEGG	ncr:NCU08762.
NMPDR	fig|5141.1.peg.8812.
Enzyme and pathway databases
BioCyc	NCRA-XX3-01:NCRA-XX3-01-003621-MON.
BRENDA	1.3.1.70. 266.
Family and domain databases
InterPro	IPR001171. Ergosterol_biosynth_ERG4_ERG24. IPR018083. Sterol_reductase_CS. [Graphical view]
Pfam	PF01222. ERG4_ERG24. 1 hit. [Graphical view]
PROSITE	PS01017. STEROL_REDUCT_1. 1 hit. PS01018. STEROL_REDUCT_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ERG24_NEUCR

Accession

Primary (citable) accession number: P38670
Secondary accession number(s): Q7RV50

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	February 1, 1995
Last modified:	March 3, 2009
This is version 67 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)