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P38670 (ERG24_NEUCR) Reviewed, UniProtKB/Swiss-Prot

Last modified December 14, 2011. Version 86. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Delta(14)-sterol reductase
EC=1.3.1.70
Alternative name(s):
C-14 sterol reductase
Sterol C14-reductase
Gene names
Name:erg-3
Synonyms:erg3
ORF Names:B2G14.010, NCU08762
OrganismNeurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987)
Taxonomic identifier367110 [NCBI]
Taxonomic lineageEukaryota › Fungi › Dikarya › Ascomycota › Pezizomycotina › Sordariomycetes › Sordariomycetidae › Sordariales › Sordariaceae › Neurospora

Protein attributes

Sequence length490 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Reduces the C14=C15 double bond of 4,4-dimethyl-cholesta-8,14,24-trienol to produce 4,4-dimethyl-cholesta-8,24-dienol.

Catalytic activity

4,4-dimethyl-5-alpha-cholesta-8,24-dien-3-beta-ol + NADP+ = 4,4-dimethyl-5-alpha-cholesta-8,14,24-trien-3-beta-ol + NADPH.

Pathway

Steroid biosynthesis; zymosterol biosynthesis; zymosterol from lanosterol: step 2/6.

Subcellular location

Membrane; Multi-pass membrane protein Probable.

Sequence similarities

Belongs to the ERG4/ERG24 family.

Ontologies

Keywords
   Biological processLipid synthesis
Steroid biosynthesis
Sterol biosynthesis
   Cellular componentMembrane
   DomainTransmembrane
Transmembrane helix
   LigandNADP
   Molecular functionOxidoreductase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological processsterol biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular componentintegral to membrane

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular functiondelta14-sterol reductase activity

Inferred from electronic annotation. Source: EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 490490Delta(14)-sterol reductase
PRO_0000207497

Regions

Transmembrane23 – 4321Helical; Potential
Transmembrane80 – 10021Helical; Potential
Transmembrane136 – 15621Helical; Potential
Transmembrane160 – 18021Helical; Potential
Transmembrane230 – 25021Helical; Potential
Transmembrane255 – 27521Helical; Potential
Transmembrane324 – 34421Helical; Potential
Transmembrane436 – 45621Helical; Potential

Sequences

Sequence LengthMass (Da)Tools
P38670 [UniParc].

Last modified February 1, 1995. Version 1.
Checksum: 9CF3104EF3014085

FASTA49054,722
        10         20         30         40         50         60 
MAGKQNQAAP KKAVAPRQQH YEFGGPIGAF GITFGLPILV HVFNLFCNDI SGCPAPSLLH 

        70         80         90        100        110        120 
PKSLDLAQLK REIGWPDNGV FGLFSWSATL WTLGYYALSL VQYRFLPGHH VEGTELSTGG 

       130        140        150        160        170        180 
RLKYKLNAFN SAMCTLAILA AGTIAQGAEF PVWTFISDNF AQIISANILF AFALAIFVYV 

       190        200        210        220        230        240 
RSFDVKPGNK DMRQLAAGGV TGSLIYDFYI GRELNPRITL PLIGQVDIKE FMEMRPGLLG 

       250        260        270        280        290        300 
WIILNCAFIA KQYRLYGYVT DSILFITAIQ AFYVFDGIYM EPAVLTTMDI TTDGFGFMLS 

       310        320        330        340        350        360 
FGDVVWVPFM YSTQTRYLSV HPQQLGAFGL IAVGAVLAAG YSIFRLSNSQ KNNFRTNPED 

       370        380        390        400        410        420 
PSVKHLTYLQ TKTGSRLITS GWWGIARHIN YLGDWLQSWP YSLPTGIAGY QILSAGSNAP 

       430        440        450        460        470        480 
GAITMLDGRE VVQGEARGWG IVFTYFYILY FAILLIHRDL RDDEKCSKKY GDDWEKYKKL 

       490 
VKWRIVPGIY

« Hide

References

« Hide 'large scale' references
[1]"The Neurospora crassa erg3 gene encodes a protein with sequence homology to both yeast sterol C-14 reductase and chicken lamin B receptor."
Papavinasasundaram K.G., Kasbekar D.P.
J. Genet. 73:33-41(1994)
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987.
[2]"What's in the genome of a filamentous fungus? Analysis of the Neurospora genome sequence."
Mannhaupt G., Montrone C., Haase D., Mewes H.-W., Aign V., Hoheisel J.D., Fartmann B., Nyakatura G., Kempken F., Maier J., Schulte U.
Nucleic Acids Res. 31:1944-1954(2003) [PubMed: 12655011] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987.
[3]"The genome sequence of the filamentous fungus Neurospora crassa."
Galagan J.E., Calvo S.E., Borkovich K.A., Selker E.U., Read N.D., Jaffe D.B., FitzHugh W., Ma L.-J., Smirnov S., Purcell S., Rehman B., Elkins T., Engels R., Wang S., Nielsen C.B., Butler J., Endrizzi M., Qui D. expand/collapse author list , Ianakiev P., Bell-Pedersen D., Nelson M.A., Werner-Washburne M., Selitrennikoff C.P., Kinsey J.A., Braun E.L., Zelter A., Schulte U., Kothe G.O., Jedd G., Mewes H.-W., Staben C., Marcotte E., Greenberg D., Roy A., Foley K., Naylor J., Stange-Thomann N., Barrett R., Gnerre S., Kamal M., Kamvysselis M., Mauceli E.W., Bielke C., Rudd S., Frishman D., Krystofova S., Rasmussen C., Metzenberg R.L., Perkins D.D., Kroken S., Cogoni C., Macino G., Catcheside D.E.A., Li W., Pratt R.J., Osmani S.A., DeSouza C.P.C., Glass N.L., Orbach M.J., Berglund J.A., Voelker R., Yarden O., Plamann M., Seiler S., Dunlap J.C., Radford A., Aramayo R., Natvig D.O., Alex L.A., Mannhaupt G., Ebbole D.J., Freitag M., Paulsen I., Sachs M.S., Lander E.S., Nusbaum C., Birren B.W.
Nature 422:859-868(2003) [PubMed: 12712197] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		X77955 Genomic DNA. Translation: CAA54919.1.
BX284753 Genomic DNA. Translation: CAD70446.1.
AABX02000017 Genomic DNA. Translation: EAA26875.1.
PIRS44170.
RefSeqXP_956111.1. XM_951018.1.
UniGeneNcr.16806.

3D structure databases

ModBaseSearch...

Protein-protein interaction databases

STRINGP38670.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiEFNCRT00000004754; EFNCRP00000004754; EFNCRG00000004748.
GeneID3872249.
KEGGncr:NCU08762.
NMPDRfig|5141.1.peg.8812.

Phylogenomic databases

eggNOGfuNOG04486.
GeneTreeEFGT00050000001699.
OMAWRILPYV.
OrthoDBEOG4R273N.

Enzyme and pathway databases

BioCycNCRA-XX3-01:NCRA-XX3-01-003621-MONOMER.

Family and domain databases

InterProIPR001171. Ergosterol_biosynth_ERG4_ERG24.
IPR018083. Sterol_reductase_CS.
[Graphical view]
KOK00222.
PfamPF01222. ERG4_ERG24. 1 hit.
[Graphical view]
PROSITEPS01017. STEROL_REDUCT_1. 1 hit.
PS01018. STEROL_REDUCT_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameERG24_NEUCR
AccessionPrimary (citable) accession number: P38670
Secondary accession number(s): Q7RV50
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: December 14, 2011
This is version 86 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents