P38661 (PDIA6_MEDSA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Probable protein disulfide-isomerase A6 EC=5.3.4.1 Alternative name(s): P5
Organism	Medicago sativa (Alfalfa)
Taxonomic identifier	3879 [NCBI]
Taxonomic lineage	Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › fabids › Fabales › Fabaceae › Papilionoideae › Trifolieae › Medicago

Protein attributes

Sequence length	364 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Catalytic activity	Catalyzes the rearrangement of -S-S- bonds in proteins.
Subcellular location	Endoplasmic reticulum lumen By similarity.
Sequence similarities	Belongs to the protein disulfide isomerase family. Contains 2 thioredoxin domains.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum
Domain	Redox-active center Repeat Signal
Molecular function	Isomerase
PTM	Disulfide bond
Gene Ontology (GO)
Biological process	cell redox homeostasis Inferred from electronic annotation. Source: InterPro glycerol ether metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	endoplasmic reticulum lumen Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	electron carrier activity Inferred from electronic annotation. Source: InterPro protein disulfide isomerase activity Inferred from electronic annotation. Source: EC protein disulfide oxidoreductase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 28	28	Potential
	Chain	29 – 364	336	Probable protein disulfide-isomerase A6		PRO_0000034243
Regions
	Domain	29 – 137	109	Thioredoxin 1
	Domain	139 – 256	118	Thioredoxin 2
Sites
	Active site	58	1	Nucleophile By similarity
	Active site	61	1	Nucleophile By similarity
	Active site	177	1	Nucleophile By similarity
	Active site	180	1	Nucleophile By similarity
	Site	59	1	Contributes to redox potential value By similarity
	Site	60	1	Contributes to redox potential value By similarity
	Site	123	1	Lowers pKa of C-terminal Cys of first active site By similarity
	Site	178	1	Contributes to redox potential value By similarity
	Site	179	1	Contributes to redox potential value By similarity
	Site	242	1	Lowers pKa of C-terminal Cys of second active site By similarity
Amino acid modifications
	Disulfide bond	58 ↔ 61		Redox-active By similarity
	Disulfide bond	177 ↔ 180		Redox-active By similarity

Sequences

	Sequence		Length	Mass (Da)
	P38661 [UniParc]. Last modified February 1, 1995. Version 1. Checksum: 7496F4093C3A01E6	FASTA	364	40,492
	10 20 30 40 50 60 MKMEMHQIWS RIALASFAFA ILFVSVSADD VVVLTEENFE KEVGHDKGAL VEFYAPWCGH 70 80 90 100 110 120 CKKLAPEYEK LPNSFKKAKS VLIAKVDCDE HKSVCSKYGV SGYPTIQWFP KGSLEPKKFE 130 140 150 160 170 180 GPRTAESLAE FVNTEGGTNV KIATAPSHVV VLTPETFNEV VLDGTKDVLV EFYAPWCGHC 190 200 210 220 230 240 KSLAPIYEKV AAVFKSEDDV VIANLDADKY RDLAEKYDVS GFPTLKFFPK GNKAGEDYGG 250 260 270 280 290 300 GRDLDDFVAF INEKSGTSRD AKGQLTSEAG IVEDLDELVK EFVAANDEEK KAVFARIEEE 310 320 330 340 350 360 VKKLEGSASR YGKIYLKVSK KYLEKGSDYA KNEIQRLERL LEKSISPAKA DELTLKKNIL STYA

References

[1]	"Molecular characterization and expression of an alfalfa protein with sequence similarity to mammalian ERp72, a glucose-regulated endoplasmic reticulum protein containing active site sequences of protein disulphide isomerase." Shorrosh B.S., Dixon R.A. Plant J. 2:51-58(1992) [PubMed: 1303795] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M80235 mRNA. Translation: AAB46930.1.
PIR	T09614.
3D structure databases
ProteinModelPortal	P38661.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR005788. Disulphide_isomerase. IPR011679. ER_p29_C. IPR005746. Thioredoxin. IPR012336. Thioredoxin-like_fold. IPR017937. Thioredoxin_CS. IPR013766. Thioredoxin_domain. [Graphical view]
Gene3D	G3DSA:1.20.1150.12. ERp29_C_type. 1 hit. G3DSA:3.40.30.10. Thioredoxin_fold. 2 hits.
Pfam	PF07749. ERp29. 1 hit. PF00085. Thioredoxin. 2 hits. [Graphical view]
PRINTS	PR00421. THIOREDOXIN.
SUPFAM	SSF47933. ERP29_C. 1 hit. SSF52833. Thiordxn-like_fd. 2 hits.
TIGRFAMs	TIGR01126. Pdi_dom. 2 hits.
PROSITE	PS00194. THIOREDOXIN_1. 2 hits. PS51352. THIOREDOXIN_2. 2 hits. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PDIA6_MEDSA

Accession

Primary (citable) accession number: P38661

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	February 1, 1995
Last modified:	September 21, 2011
This is version 75 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program